• Title/Summary/Keyword: Indirect Method

Search Result 1,753, Processing Time 0.032 seconds

Experimental infection of piglets with a field isolate of Aujeszky's disease virus in Korea: Pathogenecity, excretion, distribution and immunogenicity of virus (국내분리 Aujeszky's disease virus의 실험적 감염 자돈에 대한 바이러스학적 연구)

  • Park, Jeong-woo;Jun, Moo-hyung;An, Soo-hwan
    • Korean Journal of Veterinary Research
    • /
    • v.30 no.2
    • /
    • pp.177-186
    • /
    • 1990
  • To investigate the etiology, pathogenicity and virological properties of NYJ-1-87 strain of Aujeszky's disease virus (ADV) that was isolated from the diseased piglet in Korea, the virus at $10^{6.0}TCID_{50}/0.1ml$ was inoculated intranasally and subcutaneously into 30 to 35 days-old piglets. Results obtained through the experiments were summarized as follows. 1. Ten of the infected piglets were clinically observed for 15 days. On the 2nd day post-inoculation(pi), the signs of pyrexia, anorexia and convulsion were noted. On the 4th to 7th days pi, nervous signs of incoordination and intermittent spasm were shown in the most of piglets, and one out of 5 piglets infected intranasally was died with severe nervous signs at the 7th day pi. The signs became relieved on the 8th day pi and all of remainder were completely recovered on the 13th to 14th days pi. 2. In hematological study, prominent decrease in the number of total leukocyte and lymphocyte was shown in the ADV-infected piglets on the 6th day pi. On the 8th day pi, the cell numbers were slightly increased and returned to normal level on the 10th day pi. 3. Viral excretion of the ADV-inoculated piglets was examined by swabbing of nasal and oral cavities, and rectal feces. During the periods of the 3rd to 11th days pi, the virus was excreted intermittently from nasal and oral cavities, and rectal feces. The nasal excretions were shown the highest virus concentration of $10^{5.2}TCID_{50}/0.1ml$ at the 5th day pi. 4. Recovery of the inoculated virus from various organs of the piglets that were died or experimentally slaughtered was attempted, and the virus was isolated from the tissues of brain and tonsil by the cultured cell-inoculation method. The highest recovery rate was noted in the tonsil. By indirect immunofluorescence antibody assay using ADV-monoclonal antibody, the viral antigens were detected in tissues of spleen and liver as well as brain and tonsil on the 7th to 9th days pi. The virus was not isolated from blood and the tissues of lung and kidney throughout the experiments. 5. Titers of virus neutralizing antibody in the piglets experimentally infected with ADV became increased after the 6th to 9th days pi in both of intranasal and subcutaneous inoculation showing the highest titers of 64 to 128 on the 29th day pi. When the antibody levels were measured by radial immunodiffusion enzyme assay, the reactive diameter was enlarged to be positive after the 4th to 6th days pi in both of intranasal and subcutaneous inoculation showing the largest diameter of 13 to 14mm on the 29th day pi.

  • PDF

Characterization of Secondary Exposure to Chemicals and Indoor Air Quality in Fire Station (소방서 실내공간의 화학적 유해인자 2차노출과 실내공기질 특성)

  • Kim, Soo Jin;Ham, Seunghon;Jeon, Jeong Seok;Kim, Won
    • Fire Science and Engineering
    • /
    • v.33 no.4
    • /
    • pp.140-151
    • /
    • 2019
  • It is to assess the indoor air quality of the chemical hazardous materials exposed to the fire after firefighters returned to the fire scene. The research subject randomly selected four fire stations located in Seoul, Korea. Two fire stations were set up as control groups after the return of the firefighting activities at the actual fire scene, and two other fire stations were set up as control groups to measure the air quality of the room at normal levels regardless of the action. We conducted 24-hour monitoring for all fire accidents that occurred in Seoul Metropolitan using fire safety map computer system. Also, indoor air quality was measured immediately after homecoming if the experiment group was to be dispatched due to an accident of intermediate or larger scale. 11 hazardous substance items such as fine dust, formaldehyde, volatile organic compounds, PAH, VCM, acidity, asbestos, CO2, NO2, O3 were measured according to the process test method. Three of 11 types of harmful substances exceeded domestic and foreign standards, and one of them was found to be close to foreign standards. In particular, total volatile organic compounds, carbon dioxide and sulfuric acids were 2.5 times, 2.2 times and 1.1 times higher than the standard. Also, for formaldehyde and sulfuric acid, it was measured higher in the control group than in the case group. This findings could be used in policies to improve indoor air quality in the fire station of the Seoul Metropolitan Government.

Relation of Self Leadership and Empowerment and Organization Innovation Action in Private Security Guard (민간경비원의 셀프리더십과 임파워먼트 및 조직혁신행동의 관계)

  • Kim, Kyong-Sik;Kim, Chan-Sun
    • The Journal of the Korea Contents Association
    • /
    • v.12 no.11
    • /
    • pp.377-387
    • /
    • 2012
  • The purpose of this study is to investigate the relationship between self leadership and empowerment and organization innovation action in private security guard. This study established private security guards who is being located in Seoul, 2011 and work in the private security company by population. Using purposive sampling method, 293 samples were drawn and were used for the final analysis. Using SPSSWIN 18.0, frequency analysis, factor analysis, reliability analysis, multiple regression analysis and path analysis were performed. Cronbach's ${\alpha}$ value which shows the reliability of the questionnaire came out to be over .831. The conclusion is following. First, private security guard's self leadership affects to empowerment. That is, influence and semanticity are enlarged as action center strategy, natural compensation strategy is attained well. Also, capacity, self decision power is enlarged as constructive thinking strategy, natural compensation strategy is attained well. Second, private security guard's self leadership affects to organization innovation action. In other words, innovation action is increased as action center strategy is attained well. Also, organization's innovation result is enlarged as constructive thinking strategy, action center strategy, natural compensation strategy are attained well. Third, private security guard's empowerment affects on organization innovation action. That is, innovation action, innovation result appears high in case of influence, semanticity is enlarged. Fourth, private security guard's self leadership exerts direction indirect effect in empowerment and organization innovation action. Thus, empowerment is an important variable that mediate self leadership and organization innovation action.

Exception based Dynamic Service Coordination Framework for Web Services (웹 서비스를 위한 예외 상황 기반 동적 서비스 연결 프레임워크)

  • Han Dong-Soo;Lee Sung-Doke;Jung Jong-Ha
    • Journal of KIISE:Software and Applications
    • /
    • v.33 no.8
    • /
    • pp.668-680
    • /
    • 2006
  • Web services on the Internet are not always reliable in terms of service availability and performance. Dynamic service coordination capability of a system or an application invoking Web services is essential to cope with such unreliable situations. In dynamic service coordination, if a Web service does not respond within a specific time constraint, it is replaced with another Web service at run time for reliable invocation of Web services. In this paper, we develop an exception based dynamic service coordination framework for Web services. In the framework, all necessary information for dynamic service coordination is explicitly specified and summarized as a set of attributes. Then classes and workflows, supporting dynamic service coordination and invoking Web services, are automatically created based on these attributes. Developers of Web services client programs can make the invocations of Web services reliable by calling the methods of the classes. Some performance loss has been observed in the indirect invocation of a Web service. However, when we consider the flexibility and reliability gained from the method, the performance loss would be acceptable in many cases.

Joint Electromagnetic Inversion with Structure Constraints Using Full-waveform Inversion Result (완전파형역산결과를 구조적 제약 조건으로 이용한 고해상도 전자탐사 복합역산 알고리듬 개발)

  • Jeong, Soocheol;Seol, Soon Jee;Byun, Joongmoo
    • Geophysics and Geophysical Exploration
    • /
    • v.17 no.4
    • /
    • pp.187-201
    • /
    • 2014
  • Compared with the separated inversion of electromagnetic (EM) and seismic data, a joint inversion using both EM and seismic data reduces the uncertainty and gives the opportunity to use the advantage of each data. Seismic fullwaveform inversion allows velocity information with high resolution in complicated subsurface. However, it is an indirect survey which finds the structure containing oil and gas. On the other hand, marine controlled-source EM (mCSEM) inversion can directly indicate the oil and gas using different EM properties of hydrocarbon with marine sediments and cap rocks whereas it has poor resolution than seismic method. In this paper, we have developed a joint EM inversion algorithm using a cross-gradient technique. P-wave velocity structure obtained by full-waveform inversion using plane wave encoding is used as structure constraints to calculate the cross-gradient term in the joint inversion. When the jointinversion algorithm is applied to the synthetic data which are simulated for subsea reservoir exploration, images have been significantly improved over those obtained from separate EM inversion. The results indicate that the developed joint inversion scheme can be applied for detecting reservoir and calculating the accurate oil and gas reserves.

Estimation of Subsurface Structure and Ground Response by Microtremor (상시미동에 의한 지하구조와 지반응답의 추정)

  • Hwang, Min-Woo;Kim, Sung-Kyun
    • Journal of the Korean earth science society
    • /
    • v.23 no.4
    • /
    • pp.380-392
    • /
    • 2002
  • The purpose of the present study is to evaluate the usage of microtremor in estimation of subsurface structure and ground response to strong ground motion. To accomplish the purpose, the current status of microtremor study are reviewed and microtremors recorded at several stations are analysed. First of all, the stability of microtremor is examined through the analysis of microtremors recorded for 80 seconds per hour during the time from 10 p.m. to 6 a.m. for eight hours at night time. It is found that the shape of microtremor spectra of low frequency below 10Hz is approximately invariable with time and the spectra contain informations about subsurface structure. The subsurface structures estimated from the predominant frequency determined from the recorded microtremors are compared with the known ones from geophysical surveys at several stations in Kyungju. The comparison of structures shows rough agreements at most stations. Horizontal to vertical spectral ratio(HVSR) technique for microtremor has been proposed as an indirect method to determine ground response to strong ground motion. The HVSR for microtremors recorded in Kyungju is calculated and compared with theoretical transfer function calculated from the known structures. The comparison shows rough coincidence of the peak frequency of spectra between them.

Optical Properties of Aerosol at Gongju Estimated by Ground-based Measurements Using Sky-radiometer (스카이라디오미터(Sky-radiometer)로 관측된 공주지역 에어로솔의 광학적 특성)

  • Kwak, Chong-Heum;Suh, Myoung-Seok;Kim, Maeng-Ki;Kwak, Seo-Youn;Lee, Tae-Hee
    • Journal of the Korean earth science society
    • /
    • v.26 no.8
    • /
    • pp.790-799
    • /
    • 2005
  • We investigate the optical properties of aerosols over Gongju by an indirect method using the pound measurement, Sky-radiometer. The analysis period is from January to December, 2004. Skyrad. pack.3 is used to estimate the optical properties, such as the aerosol optical thickness (AOT), single scattering albedo (SSA), ${\AA}ngstron$ exponent $({\alpha})$ and size distribution, of aerosols from the ground measured radiance data. And qualify control is applied to minimize the cloud-contaminated data and improve the quality of analysis results. The 12-month average of AOT, ${\alpha}$, and SSA are 0.46, 1.14, and 0.91, respectively. The average volume spectra of aerosols shows a bi-modal distribution, the first peak at fine mode and the second peak at coarse mode. AOT and coarse particles clearly increases while SSA decreases during the Asian dust events. The optical properties of aerosols at Gongju vary with?seasons, but those are not influenced by the wind direction.

Effect of tetracycline-HCl root conditioning on gingival epithelial cell attachment to root surface (염산테트라싸이클린으로 처리한 치근면의 치은상피세포부착에 관한 연구)

  • Hwang, Na-Young;Park, Byung-Ki;Kim, Sang-Mok;Kuk, Jung-Ki;Park, Ju-Chul;Kim, Byung-Ock
    • Journal of Periodontal and Implant Science
    • /
    • v.32 no.1
    • /
    • pp.13-23
    • /
    • 2002
  • The ultimate goal of periodontal therapy is directed to arresting the progression of the disease, and regenerating the fibrous attachment. In order to achieve such treatment aim, the plaque and calculus must be eliminated and the physiological conditions of the root surface must be changed to facilitate the attachment and migration of the new fibroblasts, The method of changing the proper root surface conditions to promote the healing of periodontal tissue involves mechanical procedures, such as scaling and root planing, and chemical procedures such as tetracycline-HCl. However, the formation of a long junctional epithelium was most frequently observed type of healing. Thus, the aim of this study was to examine in vitro the influence of surface conditioning of dentin by TC-HCl on human gingival epithelial cell attachment. Human gingival epithelial cells were obtained from healthy retromolar pad area(under the age 23 years). Seventy two teeth extracted from severe periodontitis were used as study material. To evaluate the epithelial cell attachment to dentin, the prepared specimen was divided to four groups. For the control group, only scaling and root planing were carried out, and for the test group, 1 to 3, the concentration of the TC-HCl was 50, 125 and 250mg/ml respectively. After cell cultivation time of 1-, 3-. 24 hour, for the indirect quantitative assessment of gingival epithelial cell attached to dentin sample, the absorbance of epithelial cell unattached to dentin was measured. The results were as follows; 1. There was no statistically significant difference between scaling and root planing group and TC-HCl 50mg/ml 125mg/ml and 250mg/ml group about absorbance of unattached epithelial cell to dentin sample(p>0.5). 2. As time passes, the absorbance of unattached gingival epithelial cell to dentin sample was decreased statistically significant(p<0.05). 3. There was no statistically significant difference among the TC-HCl group(p>0.05) We concluded that there was similar effect on gingival epithelial cell attachment between TC-HCl conditioning on root surface and only scaling and root planing treatment

Laparoscopic Contralateral Exploration for Clinically Unidentified Patent Processus Vaginalis (소아 서혜부 탈장환자에서 반대측에 대한 복강경 검사의 의의)

  • Park, Il-Kyung;Mok, Woo-Kyun
    • Advances in pediatric surgery
    • /
    • v.13 no.2
    • /
    • pp.194-202
    • /
    • 2007
  • It is known that pediatric inguinal hernia is caused by the incomplete closure of processus vaginalis (PV). In the case of unilateral hernia, possibile contralateral patent PV should be considered because of its delayed appearance as well as its risk of incarceration. Direct visualization of patent PV could be done by contralateral exploration or by indirect exploration through the ipsilateral opening site of the affected hernia assisted with laparoscope. A patient group (321 persons) to whom laparoscopy was not performed from March 2000 to March 2003 was analyzed and compared with a patient group (280 persons) to whom laparoscopy was performed from April 2003 to September 2005. With all 601 patients, the sex ratio (male/female) of patients was 3.8:1. The side distribution was 57.7% in the right, 32.1% in the left and 10.1% in bilateral. There was no difference of sex and side distribution between before and after laparosopy adoption. We did not find an age correlation in natural closure of the residual PV of the peritoneum. Contralateral hernia developed in 14 persons (2.5%) after the operation of unilateral inguinal hernia before laparoscope adoption. But no contralateral hernia developed after April 2003 with laparoscopy. We think that if we use laparoscopy, being a safe and accurate method, to check whether the contralateral residual PV is opened or closed, possible future contralateral operation can be avoided.

  • PDF

Development of an Ecological Model to Improve Health Care Management for Children in Child Care Centers (보육시설 아동의 건강관리향상을 위한 생태학적 모형 개발)

  • Park, Eun-Sook;Im, Yeo-Jin;Cho, Eun-Ji
    • Child Health Nursing Research
    • /
    • v.19 no.1
    • /
    • pp.59-68
    • /
    • 2013
  • Purpose: This study was conducted to identify current health care management and barriers in health care management according to ecological systems, and to develop an ecological model for enhancing health care in child care centers. Methods: Focus group interviews were conducted with directors and teachers of child care centers, and with parents of children enrolled in child care. Data were analyzed by the latent content analysis method. Results: Twelve categories of health care management were identified. Barriers to child health care included knowledge deficit and lack of competence in health care by teachers, lack of useful health care manuals, non-existence of professional child health care personnel in child care centers, lack of mutual information sharing and disagreement on child health conditions between child care personnel and parents, lack of specific health related child care inspection criteria and time flexible child care centers with a lack of policy on collaboration with health care facilities. The ecological model developed included specific strategies to improve health care management in child care. Conclusion: The proposed ecological model to improve child health care management should be useful to plan future health care program considering both the immediate and indirect social environment surrounding children in child care.