• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.3
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• pp.333-338
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• 2008

The tea has traditionally been used as a foodstuff by unique flavor, however recently not only the diversity of consumer demands but also the public interest in unique favorite and functional aspects have increased. It has been also reported that the main components contained in the leaves of tea (Camellia sinensis) include total nitrogen, free amino acids, polyphenols, and fiber, of which catechin has powerful bioactive effect such as anti-cancer, anti-aging, and anti-diabetic. (-)-Epigallocatechin gallate (EGCG) which is a major phenolic constituent of green tea extract has received considerable attention for a variety of important bioactivities. This study was carried out to obtain useful information for tea breeding programs, and to investigate the concentration of quality and functional related components in Korean indigenous tea germplasms. Korean indigenous tea lines were classified into three groups of sprout time, i.e, early, medium and late sprout time, and the ratio were 20%, 43% and 37%, respectively. There was a difference in characteristics among these Korean indigenous tea lines, leaf width of those ranged from 19.8 to 75 mm, leaf length was 35.5-160.0 mm, and leaf area was $660-8,400\;mm^2$. Experimental data on chlorophyll content (SPAD value) of Korean indigenous tea genetic resources ranged from 51.3 to 82.3. The concentrations of the total nitrogen, total free amino acids, and theanine were ranged 4.18-6.07%, 2.87-4.58%, and 1.64-2.66%, respectively. Also, catechin concentration showed from 11.54 to 15.07%, and concentration of caffeine was 2.82-4.23%. These results indicated indicated that it is possible to select elite lines with high concentration of quality related components and low concentration of caffeine from Korean domestic tea germplasms.

• Korean Journal of Poultry Science
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• v.50 no.4
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• pp.241-249
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• 2023

Following the Animal genetic resource for food and agriculture (AnGR) is considered as an independent resource for the possessing country, ensuring the sovereignty of AnGR is important. The present study investigated the growth and egg production performance of six breed enrolled in DAD-IS for the purpose of securing scientific data on AnGR in Korea. A total of 323 chickens (female 181, male 142) were used in this study, with the following six breeds: Korean Leghorn (LEG), Gyeongbuk Araucana (ARA), Korean native chicken (KNC), Korean Ogye (Ogye), Hyunindak (HIL), Heongseongyakdak (HYD). The body weight of male ARA from hatching to 32 weeks of age was the highest among the breeds, and LEG and Ogye were relatively lower (P<0.0001, excluded body weight data of HYD). The body weight of female ARA was the highest and HYD was significantly the lowest among the female chicken breeds (P<0.0001). The laying percentage was the highest in LEG and was the lowest in HYD among the breeds (P<0.0001). The average egg weight from 20 to 40 weeks of age was the highest in ARA, followed by LEG and was the lowest in Ogye (P<0.0001), and the adapted breed including LEG and ARA was higher than indigenous breed (P<0.05). Egg mass production was the highest in Korean Leghorn. Collectively, these results show that ARA has the best growth ability, and LEG has the best egg production performance among the used breeds. This suggests that the adapted breed with high commercial performance is important AnGR in Korea.

• Reproductive and Developmental Biology
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• v.39 no.1
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• pp.13-21
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• 2015

The importance of genetic resource preservation has been highlighted in the literature as a means of maintaining genetic diversity. Investigations for hematologic values and the differential count of white blood cell count (WBC) for Korean indigenous cattle (KIC) and endangered indigenous cattle (EIC) are rarely performed. Therefore, the objective of this study was to investigate the hematologic values of total 40 EIC (White, Black, Mini cattle) and 35 KIC as control by analysis of hematologic characteristics. As a result, the mean values of RBC and platelet of EIC were significantly decreased by age (p<0.05). The mean values of RBC, HCT, MCV and MCHC between EIC and KIC of the same age (2~3 years) showed the statistical significance (p<0.05). Also, in the WBC of EIC, the mean values were decreased according to the age from $13.9{\times}10^3/{\mu}L{\sim}12.7{\times}10^3/{\mu}L$ under 1 year to $9.1{\times}10^3/{\mu}L{\sim}11.5{\times}10^3/{\mu}L$ over 2 years respectively. In the differential count of WBC of EIC (White, Black, Mini cattle), it showed generally the rates of 40.2%, 52.2%, 49.0% lymphocyte and 27.2%, 33.9%, 32.0% segmented neutrophil from 2~3 years respectively. Result of this study will be used for establishing reference range for blood analysis in EIC such as white, black and mini cattle. This study reported hematological values which could serve as baseline information for comparison in conditions of nutrient deficiency, physiological and health status of endangered Korean native cattle. In addition, this study provides a valuable resource for further investigations of the preservation of rare genetic stocks underlying traits of interest in cattle.

• Food Science of Animal Resources
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• v.33 no.6
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• pp.715-722
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• 2013

Heugu (Korea Black Cattle) is one of the indigenous cattle breeds in Korea; however there has been severe lack of genomic studies on the breed. In this study, we report the first whole genome resequencing of Heugu at higher sequence coverage using Illumina HiSeq 2000 platform. More than 153.6 Giga base pairs sequence was obtained, of which 97% of the reads were mapped to the bovine reference sequence assembly (UMD 3.1). The number of non-redundantly mapped sequence reads corresponds to approximately 28.9-fold coverage across the genome. From these data, we identified a total of over six million single nucleotide polymorphisms (SNPs), of which 29.4% were found to be novel using the single nucleotide polymorphism database build 137. Extensive annotation was performed on all the detected SNPs, showing that most of SNPs were located in intergenic regions (70.7%), which is well corresponded with previous studies. Of the total SNPs, we identified substantial numbers of non-synonymous SNPs (13,979) in 5,999 genes, which could potentially affect meat quality traits in cattle. These results provide genome-wide SNPs that can serve as useful genetic tools and as candidates in searches for phenotype-altering DNA difference implicated with meat quality traits in cattle. The importance of this study can be further pronounced with the first whole genome sequencing of the valuable local genetic resource to be used in further genomic comparison studies with diverse cattle breeds.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.4
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• pp.529-533
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• 2003

The intake, dry matter (DM) digestibility, total mean retention time (TMRT) and weight changes were investigated in four fat tailed and four Dorper sheep fed on a maize stover crop residue. Animals were subjected to five dietary treatments, which were: 90, 135 and $180g\;DM/kgBW^{0.75}/day$ of chopped maize stover offered with urea, and in subsequent dietary treatments $180g\;DM/kgBW^{0.75}/day$ of stover supplemented with either dried bean husks or poultry manure. Although not statistically different (p>0.05) fat tailed sheep had higher maize stover intake than the Dorper sheep when expressed per metabolic body weight. The DM intake and digestibility increased significantly (p<0.001) in both sheep breeds when the maize stover offer levels were increased from 90 to $135g/kgBW^{0.75}/day$. Dorper sheep had significantly (p<0.001) higher DM digestibility and longer total mean retention times for both the liquid and solid matter than the fat-tailed sheep. Fat-tailed sheep gained 4.75 kg while Dorper sheep gained 3.75 kg over the entire experimental period. The weight change per dietary treatment was affected by the amount of maize stover and the type and amount of supplement consumed. The higher DM digestibility and longer total mean retention time in Dorper sheep shows that the breed has adapted to utilize the low quality roughage diets. An indigenous fat tailed, has the potential to ingest high volumes of fibrous feed and has higher voluntary intakes relative to its body size. The two sheep breeds are a valuable genetic resource in sub Saharan Africa that is characterized by long dry season with no readily available and affordable conventional feeds for small ruminants.

• Korean Journal of Poultry Science
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• v.39 no.2
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• pp.105-112
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• 2012

Genetic resource conservation of indigenous species is important to conserve terrestrial, aquatic and avian species throughout the world. The native Korean Oge (KO) chickens are important and protected indigenous avian species in Korea. This KO breed is very popular in Korea because of its external appearances of blackish color. The characterization of morphology and growth performances of KO were performed since 1980s, however the information is still not sufficient for breed selection. In this study, we compared various growth performances including body growth, tibia length, shank length, chest width, chest grith and length of tail feather between male and female chickens of KO and widely known White Leghorn (WL) breeds at 1 week, 2 weeks, 5 weeks, 10 weeks, and 24 weeks. We observed differences on various growth performances at different aged groups between KO and WL chickens. This study may help for the selection of chicken breeds based on age, body growth and meat production.

• Journal of Embryo Transfer
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• v.29 no.1
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• pp.29-33
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• 2014

Accurate information on the genetic and phenotypic characteristics and diversity of the indigenous Farm Animal Genetic Resources (FAnGR) is the basis on which their present and future sustainable utilization and conservation should be made. The paper describes the objectives, structure, functionality, content, utility and future prospects of the Country-Domestic Animal Genetic Resources Information System (DAGRIS) of ILRI. This electronic database is designed to cater for the needs of researchers, policy makers, development practitioners, teachers, students and farmers in developing countries for efficient access to available published and grey literature from past and present research results on the origin, distribution, diversity, present use and status of selected Farm Animal Genetic Resources (FAnGR) of the countries. Development of the country-modules of c-DAGRIS in English and French for Anglophone and Francophone countries is finalized and ready to be used.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.5
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• pp.616-627
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• 2014

The Lanyu is a miniature pig breed indigenous to Lanyu Island, Taiwan. It is distantly related to Asian and European pig breeds. It has been inbred to generate two breeds and crossed with Landrace and Duroc to produce two hybrids for laboratory use. Selecting sets of informative genetic markers to track the genetic qualities of laboratory animals and stud stock is an important function of genetic databases. For more than two decades, Lanyu derived breeds of common ancestry and crossbreeds have been used to examine the effectiveness of genetic marker selection and optimal approaches for individual assignment. In this paper, these pigs and the following breeds: Berkshire, Duroc, Landrace and Yorkshire, Meishan and Taoyuan, TLRI Black Pig No. 1, and Kaohsiung Animal Propagation Station Black pig are studied to build a genetic reference database. Nineteen microsatellite markers (loci) provide information on genetic variation and differentiation among studied breeds. High differentiation index ($F_{ST}$) and Cavalli-Sforza chord distances give genetic differentiation among breeds, including Lanyu's inbred populations. Inbreeding values ($F_{IS}$) show that Lanyu and its derived inbred breeds have significant loss of heterozygosity. Individual assignment testing of 352 animals was done with different numbers of microsatellite markers in this study. The testing assigned 99% of the animals successfully into their correct reference populations based on 9 to 14 markers ranking D-scores, allelic number, expected heterozygosity ($H_E$) or $F_{ST}$, respectively. All miss-assigned individuals came from close lineage Lanyu breeds. To improve individual assignment among close lineage breeds, microsatellite markers selected from Lanyu populations with high polymorphic, heterozygosity, $F_{ST}$ and D-scores were used. Only 6 to 8 markers ranking $H_E$, $F_{ST}$ or allelic number were required to obtain 99% assignment accuracy. This result suggests empirical examination of assignment-error rates is required if discernible levels of co-ancestry exist. In the reference group, optimum assignment accuracy was achievable achieved through a combination of different markers by ranking the heterozygosity, $F_{ST}$ and allelic number of close lineage populations.

• Journal of Ecology and Environment
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• v.36 no.3
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• pp.159-166
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• 2013

Oryzias latipes and Oryzias sinensis are indigenous species found in Japan, China, and other East Asian countries, including Korea. Based on morphological differences, the species have been classified distinctly. However, the range of morphological characters such as the number of gill rakers, vertebrae, and spots on the lateral body overlaps and is too vague for clear identification, so their classification based on their morphological characteristics remains uncertain. In this study, the mitochondrial cytochrome oxidase subunit I (COI) gene, which is used for DNA barcoding, was applied to clarify interspecific variation of O. latipes and O. sinensis. Intraspecific genetic diversity was calculated to identify correlations with geographic distributions. We studied two species collected from 55 locations in Korea. All individuals carried a 679-base pair gene without deletion or insertion. Between species, 525 base pairs of the gene were shared. The Kimura two parameter (K2P) distance of O. latipes and O. sinensis was 0.41% and 1.39%, respectively. Mean divergence within genera was 23.5%. Therefore, the species were clearly different. The distance between O. latipes and O. sinensis was 14.0%, which is the closest within genera. Interestingly O. latipes from the Japanese and Korean group represented 16.5% distant. These results were derived from geohistorical and anthropogenic environmental factors. The O. latipes haplotypes were joined in only one group, but O. sinensis was divided into two groups, one is found in the Han River and upper Geum River watershed; the other is found in the remaining South Korean watersheds. Further studies will address the causes for geographic speciation of O. sinensis haplotypes.

• Journal of Environmental Policy
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• v.6 no.1
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• pp.41-60
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• 2007

Attempts were made to define the elements of debates, impact of decisions of the Access to Genetic Resources and Benefit-sharing(ABS) of the Conference of the Parties(COP) to the Convention on Biological Diversity(CBD) In Korea. Providing policy suggestions to cope with ABS, a cross-cutting issue of the meetings of the COP, was also undertaken. Meetings concerning ABS deal with several key matters such as an international regime, which is a legally binding implementation tool of the Bonn Guidelines, an international certificate of genetic resources' origin/source/legal provenance, and disclosure of origin of genetic resources, compliance measures with prior informed consent of the Contracting Parties providing such resources and with mutually agreed terms on which access was granted. Developing countries, rich in biodiversity and genetic resources, use the CBD as a major tool to maximize their national profits. They demand for national sovereign rights for the genetic resources and indigenous communities providing associated traditional knowledge. At the meetings of the COP, in addition, they requested that developed countries should transfer technologies and provide a financial mechanism for resource conservation to them. On the contrary, the developed countries argue that facilitating access to genetic resources is essential for scientific research and development, and that both Intellectual Property Rights and biotechnology using genetic resources should be protected to maximize their national benefits. Decisions of the COP concerning the Bonn Guidelines and compliance measures with ABS will affect on various socioeconomic fields of Korea, a country which is short of genetic resources. Especially, the importation of genetic resources and land development which might damage genetic resources will be limited seriously. Consequently, overall expenses will increase for the securing genetic resources from the foreign countries and developing biotechnology for conservation and sustainable uses of genetic resources. To minimize the adverse impacts, we endeavor to establish our clear standpoint and to lead the international trends, which are favorable for us. In order to achieve these objectives, government needs i) to proceed researches to lead the international ABS debates actively and to prepare the expected decisions of the future meetings of the COP, ii) to establish a national implementation plan to cope with the ABS and its related decisions, iii) to examine and improve the efficiencies of the national implementation plan with a proper monitoring system, and iv) cope with the other international meetings including the meetings of Trade Related Intellectual Properly Rights and International Treaty on Plant Genetic Resources for Food and Agriculture actively.