• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3791-3794
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• 2012

CD44v, especially splice variants containing exon v6, has been shown to be related closely to development of different tumors. High levels of CD44v6/v7 have been reported to be associated with invasiveness and metastasis of many malignancies. The objective of this study was to detect expression of CD44v6-containing variants in patients with acute promyelocytic leukemia (APL) and evaluate the potential of CD44v6/v7 for risk stratification. Reverse transcription polymerase chain reaction (RT-PCR) followed by PCR product purification, ligation into T vectors and positive clone sequencing were used to detect CD44 v6-containing variant isoforms in 23 APL patients. Real-time quantitative PCR of the CD44v6/v7 gene was performed in patients with APL and in NB4 cells that were treated with all-trans retinoic acid (ATRA) or arsenic trioxide ($As_2O_3$). Sequencing results identified four isoforms (CD44v6/v7, CD44v6/v8/v10, CD44v6/v8/v9/v10, and CD44v6/v7/v8/v9/v10) in bone marrow mononuclear cells of 23 patients with APL. The level of CD44v6/v7 in high-risk cases was significantly higher than those with low-risk. Higher levels of CD44v6/v7 were found in three patients with central nervous system relapse than in other patients inthe same risk group. Furthermore, in contrast to ATRA, only $As_2O_3$ could significantly down-regulate CD44v6/v7 expression in NB4 cells. Our data suggest that CD44v6/v7 expression may be a prognostic indicator for APL.

• 한국어류학회지
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• 제22권1호
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• pp.17-24
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• 2010

본 연구에서는 감성돔의 염분과 수온 변화에 따른 스트레스 반응을 알아보기 위하여 glucocorticoid receptor (GR) mRNA 발현을 조사하였다. 감성돔 신장으로부터 전장의 GR cDNA를 클로닝하였고, 염분과 수온이 변화하는 동안 아가미, 신장 및 장에서 GR mRNA 발현 변화를 quantitative real-time PCR (QPCR)을 이용하여 조사하였다. 염분 변화시, 아가미, 신장 및 장에서 GR mRNA 발현은 0 psu에서 가장 높게 나타났으며, 혈장 cortisol과 glucose 농도도 증가한 반면, triiodothyronine ($T_3$) 농도는 감소하였다. 수온 변화시, 아가미, 신장 및 장에서 GR mRNA 발현은 $30^{\circ}C$에서 가장 높게 관찰되었다. 혈장 cortisol, glucose 및 $T_3$ 농도 또한 고수온 ($30^{\circ}C$)에서 증가하였다. GR mRNA 발현의 증가는 염분과 수온 변화와 같은 환경 요인에 대한 좋은 스트레스 지표로 여겨진다.

• 한국자원식물학회지
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• 제33권4호
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• pp.337-364
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• 2020

강원도에 위치한 태기산(1,261.4 m)은 식물지리학적으로 매우 중요한 위치를 차지하고 있으나 외래식물의 유입, 산림의 개발 등으로 산림 훼손되고 심해지고 있다. 본 연구는 태기산의 정확한 관속식물상을 파악하기 위하여 2008년 4월부터 동년 8월, 2018년 3월부터 동년 10월에 걸쳐 총 21회의 현지조사를 실시하였으며, KH에 석엽표본으로 소장되어 있는 태기산 일대의 표본을 추가하여 목록을 작성하였다. 그 결과, 96과 338속 580종 4아종 66변종 12품종으로 총 662분류군이 확인되었다. 한반도 특산식물은 28분류군이었으며, 산림청지정 희귀식물은 총 8분류군, 적색목록은 11분류군이 조사되었다. 환경부지정 식물구계학적 등급종으로는 127분류군이며, 침입외래식물은 생태계 교란종인 애기수영을 포함하여 총 45분류군으로 나타났다.

• 한국발생생물학회지:발생과생식
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• 제21권2호
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• pp.193-204
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• 2017

The influence of triploidization on histological characteristics of retina, trunk kidney, liver and midgut tissue, and cell cycle of tail fin and gill tissue in far eastern catfish, Silurus asotus were analyzed. In the infertile triploid fish, the nucleus and/or cell size of secondary proximal tubule cells of trunk kidney, hepatocyte and midgut epithelium are much larger than those of the corresponding cells in the diploid fish (P<0.05). However, triploid tissue showed fewer number of outer nuclear layer in retina and nuclei in secondary proximal tubule of trunk kidney than those for diploid tissue. The mean percentages of the $G_l-$, the S- and the $G_2+M-phase$ fractions were 92.5%, 3.2% and 4.3% in tail fin tissue of diploid, and 93.4%, 2.6% and 4.0% in those of triploid, respectively. There were no significant differences in the percentages of each cell cycle fraction between diploid and triploid. The mean percentages of each phase fractions were 75.1%, 11.1% and 13.8% in gill tissue of diploid and 85.2%, 8.9% and 5.9% in those of triploid, respectively. The differences of cell cycle between tail fin tissue and gill tissue were statistically significant in diploid and triploid (P<0.05). Also, the differences between diploid and triploid were statistically significant in tail fin tissue and gill tissue (P<0.05). Cyclin D1 and cyclin E expressions were not significantly difference between gill tissue and tail fin tissue, and protein expressions of induced triploid were higher than those of diploid. Results from this study suggest that some characteristics in the triploid exhibiting larger cell and nucleus size with fewer number of cell than diploid can be used as an indicator in the identification of triploidization and ploidy level in far eastern catfish.

• 한국수정란이식학회지
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• 제28권3호
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• pp.237-241
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• 2013

It is generally accepted that chronic stress impairs female reproduction. It negatively affects ovarian function and the number of ovulated oocytes. Chronic stress lowers the number of retrieved oocytes. Ovarian follicular development is regulated by both pituitary-derived gonadotropins and intraovarian regulatory factors. The main corticosteroids are cortisol, cortisone, 11-deoxycortisol and corticosterone, cortisol being one of the most commonly used welfare and stress physiological indicator. In this study, we investigated the effect of cortisol level on progesterone patterns and ovulation in the dog. Cortisol and progesterone level of serum were analyzed by radioimmunoassay. The day of ovulation was considered as the day when serum progesterone concentration was 6.0~8.0 ng/ml. In vivo dog oocytes were collected by flushing oviducts of mixed-breed bitches at three days after ovulation. We classified dogs as having group 1 (cortisol level, 0 ${\leq}$ or < $2{\mu}g/dl$), group 2 (corisol level, 2 ${\leq}$ or < $4{\mu}g/dl$), group 3 (cortisol level, 4 ${\leq}$ or < $6{\mu}g/dl$) and group 4 (cortisol level, $6{\mu}g/dl$ ${\leq}$). The patterns of progesterone were not different in four cortisol groups. The average numbers of retrieved oocytes was not different in four cortisol groups. These results suggest that different cortisol levels on estrus dogs do not affect ovulation, number of ovulated oocytes and progesterone changes.

• 한국해양학회지
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• 제15권1호
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• pp.17-33
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• 1980

The materials were obtained in the eastern Gulf of Alaska and the south- eastern Bering Sea during the cruise of the research vessel, Ohdae San, from July to October 1978. A total of 76 samples were taken by NORPAC net from a depth of 200 meters or less in coastal areas. 1. The surface water temperature in the coastal waters, varing from 9 to 10$^{\circ}C$, was lower than that in offshore waters which varied from 10 to 12.9$^{\circ}C$ in the eastern Gulf of Alaska. Thermocline was formed in the 30∼50 meter layer. Salinity of the coastal waters of Kenai Peninsula and Kodiak was 30 which was slightly lower than that of offshore. 2. The water temperature of the surface layer down to 30 meters varied from 7 to 10$^{\circ}C$ and from 1 to 9$^{\circ}C$ in the layer below 30 meters in the south-eastern Bering Sea. Meandering thermal front spread from the Alaska Peninsula to St. Matthew Island by way of St. Paul, and a thermocline was found at the 30∼50 meter layer Salinity ranged from 31.0 to 33.0 and that of northern and coastal waters was little lower than that of offshore. 3. Zooplankton biomass fluctuated from 0.1 to 23.6cc/10㎥ in the eastern Gulf of Alaska and 2.0 to 26.1cc/10㎥ in the south-eastern Bering Sea. Plankton was rich in the following areas, the inshore Kodiak waters, the northern Bering Sea, the Coastal waters and waters adjacent to Alutian islands however, poor in the central Bering Sea. In general, the south-eastern Bering Sea has a higher concentration of plankton volume than the eastern Gulf of Alaska. 4. Twenty three species representing 17 genera of copepods were identified from the samples. These were mostly composed of the cold water species, such as Pseudocalanus minutus, Acartia longiremis, Metridia lucens and Eucalanus bungii var. bungii. 5. The cold oceanic species were composed of Calanus cristatus, C.plumchrus, Metridia lucens, Eucalanus bungii var. bungii and Scolecithricella minor. The cold neritic species were Centropages abdominalis, Pseudocalanus minutus, Acartia longiremis, Eurytemora herdmanii, Pontella pulvinata, P. longipedata and Tortanus discaudatus. On the other hand, the warm oceanic species were Calanus tenuicornis and Oithona plumifera. The cosmopolitan species were Calanus finmarchicus and Oithona similis. 6. It was suggested that the cold oceanic species, Eucalanus bungii var. bungii and Metridia lucens in the south-eastern Bering Sea can be recommended as a valuable indicator species for finding the fishing grounds of demersal fish such as pollock and yellowfin sole in this area.

• 한국식품영양학회지
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• 제27권5호
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• pp.940-955
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• 2014

The purpose of this study was to compare the degree nutrient intake, health status and other characteristics of females aged 65 years and over in a longevity area according to family arrangement. For analysis, 585 female elderly were recruited from the Kugoksoondam area (Kurye, Goksung, Soonchang and Damyang counties), known as the longevity-belt region in Jeonla province, Korea. The subjects were categorized into three groups according to family arrangement (living alone, living with spouse only and living with family). Demographic characteristics were collected, as well as information on physical measurements, blood tests for biochemical indicators, health status and health-related life style, dietary behavior, favorite food groups, consumption frequency of food groups, nutrient intake and mini nutrition assessment. In the group living with their spouse only, the highest education, physical activity, diverse food intake, frequency of eating meats and fish, energy and nutrients intake, and score on the mininutrient status assessment (MNA) were found to be significantly favorable factors. Taken together, these results demonstrated that the group living with their spouse only had relatively superior nutrient intake and the quality of diet. In contrast, the group living alone showed the lowest self-rated economic status, diversity of food intake, and physical activity, with the highest frequency of drinking, smoking and regular exercise for almost everyday compared with the other groups. On the other hand, those living with family demonstrated the lowest intake of supplements or functional foods, and levels of hemoglobin hematocrit MCH, but the highest HBA1c and blood sugar. Therefore, the group living with family was assumed to be at risk of anemia and diabetes. These results could be useful to plan effective strategies to increase the health-life expectancy of Korean elderly people living in rural areas, according to family arrangement.

• 대한소아치과학회지
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• 제35권2호
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• pp.243-258
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• 2008

본 연구는 성장 중인 부정교합 환자의 진단이나 치료계획의 수립, 치료방법 및 시기, 예후 등을 결정하는데 중요한 판단 지침이 되는 치아 석회화 단계와 경추 및 수완부골 성숙도간의 연관성을 규명하여 개인의 보다 정확하고 효율적인 사춘기 성장 정도를 평가하기 위해 시행하였다. 전신 질환이 없고 교정 치료 경험이 없는 7-16세의 여아 154명, 남아 179명, 총 333명의 파노라마 방사선사진, 측모 두부규격 방사선사진, 수완부 방사선사진을 분석하여 평가하였다. 연구 결과, 파노라마 방사선사진의 치아 석회화 단계를 통하여 사춘기 성장 정도를 평가하는 것은 부정교합 환자의 진단 및 치료계획을 수립하는데 임상적으로 유용한 정보를 제공할 것으로 판단되었다.

• 한국유통학회지:유통연구
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• 제16권5호
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• pp.43-61
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• 2011

본 연구에서는 16개 광역자치단체의 전통시장 관련 자료를 바탕으로 전통시장지원정책의 효과성과 효율성을 실증적으로 살펴보고자 하였다. 전통시장 지원정책의 목표를 시설개선이 아닌 전통시장의 매출액 및 고객증가라고 할 때 기존의 지원방식은 이러한 지원목표와 상당한 괴리가 있음을 상관분석을 통해 확인할 수 있었다. 또 DEA모형의 효율성 분석을 통해 전통시장지원이 기존의 시설개선사업에서 탈피하여 산출변수와 실질적인 관련성이 높은 사업부문으로의 전환될 경우 어떻게 효율적인 지원이 가능할 것인가에 대해 논의하였다. 매출액증대의 경우에는 고객안내센터, 원산지표시, 자율포장대, 방송시설과 같은 분야의 지원이 보다 효과적이며 방문고객증대의 경우에는 고객안내센터, 방송시설, 방송광고, 경품행사와 같은 분야의 지원이 요구됨을 알 수 있었다. 구체적인 지원 방안으로 인천지역의 시장 상인과 소비자 실태 조사한 자료를 바탕으로 다양한 상품 개발과 교차 판매, 고객 친화적인 고객정보센터 운영, 상품권 활성화, 시장 정보시스템 구축 등의 방안을 제시하였다.

• 한국수자원학회논문집
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• 제44권12호
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• pp.975-990
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• 2011

본 연구에서는 하상의 시공간적변동에 대한 평가기법을 개발하고자, 1차원HEC-RAS 모델과 2차원CCHE2D 모델을 이용해 하상변동을 평가하였다. 실제하천의 장기간 하상변동의 결과를 해석함에 있어서 변동 특성을 일관적인 기준으로 평가하고, 변화량을 직관적으로 알 수있는 평가방법을개발하고자유사단면누가곡선, 유사단면모멘트, 유사확률분포함수의 세 가지 평가 방법을 개발하였다. 본 연구를 수행한 결과 유사단면 누가곡선법은 단면의 총량적 개념의 침식 또는 퇴적 여부를 판별하는데 효과적이며, 유사단면 모멘트법은 하천의 좌, 우안을 나누는 기준선을 중심으로 좌, 우안의 퇴적 또는 침식 정도를 하나의 척도로 제시하는 것이 가능하였다. 이러한 방법은 하천의 곡률 등과 연계한 하상변동 예측에 있어서 하나의 영향지표로 활용할 수 있을 것이다. 유사확률분포함수의 경우 하천 단면 구간에 대한 시간의 변화에 따른 변동 모의 결과를 직관적으로 도시하고, 해당 구간의 시공간적 변동 특성을 통계적 특성치인 평균과 신뢰 구간을 통해 정량화 할 수 있었다.