• Animal Bioscience
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• 제37권5호
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• pp.795-806
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• 2024

Objective: Karan Fries (KF), a high-producing composite cattle was developed through crossing indicine Tharparkar cows with taurine bulls (Holstein Friesian, Brown Swiss, and Jersey), to increase the milk yield across India. This composite cattle population must maintain sufficient genetic diversity for long-term development and breed improvement in the coming years. The level of linkage disequilibrium (LD) measures the influence of population genetic forces on the genomic structure and provides insights into the evolutionary history of populations, while the decay of LD is important in understanding the limits of genome-wide association studies for a population. Effective population size (N_e) which is genomically based on LD accumulated over the course of previous generations, is a valuable tool for e valuation of the genetic diversity and level of inbreeding. The present study was undertaken to understand KF population dynamics through the estimation of N_e and LD for the long-term sustainability of these breeds. Methods: The present study included 96 KF samples genotyped using Illumina HDBovine array to estimate the effective population and examine the LD pattern. The genotype data were also obtained for other crossbreds (Santa Gertrudis, Brangus, and Beefmaster) and Holstein Friesian cattle for comparison purposes. Results: The average LD between single nucleotide polymorphisms (SNPs) was r² = 0.13 in the present study. LD decay (r² = 0.2) was observed at 40 kb inter-marker distance, indicating a panel with 62,765 SNPs was sufficient for genomic breeding value estimation in KF cattle. The pedigree-based N_e of KF was determined to be 78, while the N_e estimates obtained using LD-based methods were 52 (SNeP) and 219 (genetic optimization for N_e estimation), respectively. Conclusion: KF cattle have an N_e exceeding the FAO's minimum recommended level of 50, which was desirable. The study also revealed significant population dynamics of KF cattle and increased our understanding of devising suitable breeding strategies for long-term sustainable development.

• Journal of Microbiology and Biotechnology
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• 제34권5호
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• pp.1146-1153
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• 2024

The increasing economic losses associated with growth retardation caused by Enterocytozoon hepatopenaei (EHP), a microsporidian parasite infecting penaeid shrimp, require effective monitoring. The internal transcribed spacer (ITS)-1 region, the non-coding region of ribosomal clusters between 18S and 5.8S rRNA genes, is widely used in phylogenetic studies due to its high variability. In this study, the ITS-1 region sequence (~600-bp) of EHP was first identified, and primers for a polymerase chain reaction (PCR) assay targeting that sequence were designed. A newly developed nested-PCR method successfully detected the EHP in various shrimp (Penaeus vannamei and P. monodon) and related samples, including water and feces collected from Indonesia, Thailand, South Korea, India, and Malaysia. The primers did not cross-react with other hosts and pathogens, and this PCR assay is more sensitive than existing PCR detection methods targeting the small subunit ribosomal RNA (SSU rRNA) and spore wall protein (SWP) genes. Phylogenetic analysis based on the ITS-1 sequences indicated that the Indonesian strain was distinct (86.2% nucleotide sequence identity) from other strains collected from Thailand and South Korea, and also showed the internal diversity among Thailand (N = 7, divided into four branches) and South Korean (N = 5, divided into two branches) samples. The results revealed the ability of the ITS-1 region to determine the genetic diversity of EHP from different geographical origins.

• 헤리티지:역사와 과학
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• 제48권3호
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• pp.186-203
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• 2015

이 글은 실크로드라는 이름으로 더 많이 알려진 중앙유라시아 미술사 연구의 성과와 과제에 대해 살펴본 것이다. 이 글에서 다루는 '중앙유라시아'의 공간 범주는 중국 신강, 티베트, 몽골, 러시아 및 구소련 공화국, 인도 북부, 이란, 터키이며, 30년 이상 거쳐 온 이 지역 미술사 연구의 지적 모색 과정을 크게 되짚어 보고, 이를 통해 앞으로 나아가야할 바를 생각해보려 하였다. 한국의 중앙유라시아 미술사 연구는 학문적 연륜은 짧지만, 2015년 현재 인도서북부, 중국 신장, 돈황 등 몇몇 분야의 괄목할만한 발전이 있었다. 발전의 뒤에는 1990년 중앙유라시아로 현지답사가 가능해지면서 실견이 가능해진 환경도 있지만, 중앙아시아학회, 서울대 중앙유라시아연구소와 같은 연구기관이 후학들에게 마련해 준 '학문의 장(場)'이 있었다. 두 학술단체는 비록 느리지만 꾸준히 중앙유라시아 미술사 관련 연구로 학자들의 관심을 유도하고 연구자들을 배출해냈다. 2012년 이후로는 갑자기 상황이 많이 바뀌었다. 관이 주도하는 국제학술대회가 우후죽순처럼 개최되었기 때문이다. 실크로드를 한반도로 연장하고, 궁극에는 '중앙유라시아의 이니셔티브'를 확보하려는 것이다. 2015년 현재, 대중들의 관심은 높아졌고, 1980년 이후 두 번째 열풍이 불고 있다. 물론 최근의 국제학술대회가 목표와 지향점이 미리 설정되어 있어 객관성이 담보되거나 발표자가 자신의 목소리를 내기는 어렵다는 문제가 있기는 하지만, 틀림없는 사실은 관심이 높아진 만큼 발전 가능성이 커졌다는 것이다. 지금 이 시점에서 무엇보다 중요한 것은 말할 필요도 없이 중앙유라시아 미술사를 체계적이고 전문적으로 연구할 신진 연구자의 양성이다. 한국문화와 세계 문화가 만나 소통하고 이를 기반으로 신개념 문화를 만들어 내려면 말이다. 위의 과제가 해결되지 않는다면 한국 유라시아 미술사 연구는 자칫 오랫동안 공회전할 가능성이 있다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 추계학술대회
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• pp.5-5
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• 2019

The objectives of this paper are (i) to analyze past trends and future directions of rice production, consumption and trade across the world and (ii) to discuss emerging challenges and future directions in the global rice industry. Rice is a staple food of over half of the world's 7.7 billion people. It is an important economic, social, political, and cultural commodity in most Asian countries. Rice is the $1^{st}$ most widely consumed, $2^{nd}$ largely produced, and $3^{rd}$ most widely grown food crop in the world. It was cultivated by 144 million farms in over 100 countries with harvested area of over 163 million ha producing about 745 million tons paddy in 2018. About 90% of the total rice is produced in Asia. China and India, the biggest rice producers, account for over half of the world's rice production. Between 1960 and 2018, world rice production increased over threefold from 221 to 745 million tons (2.1% per year) due to area expansion from 120 to 163 million ha (0.5% per year) and paddy yield increase from 1.8 to 4.6 t/ha (1.6% per year). The Green Revolution led massive increase in rice production prevented famines, provided food for millions of people, reduced poverty and hunger, and improved livelihoods of millions of Asians. The future increase in rice production must come from yield increase as the scope for area expansion is limited. Rice is the most widely consumed food crop. The world's average per capita milled rice consumption is 64 kilograms providing 19% of daily calories. Asia accounted for 84% of global consumption followed by Africa (7%), South America (3%), and the Middle East (2%). Asia's per capita rice consumption is 100 kilograms per year providing 28% of daily calories. The global and Asian per capita consumption increased from the 1960s to the 1990s but stable afterward. The per capita rice consumption is expected to decline in Asia but increase outside Asia especially in Africa in the future. The total milled rice consumption was about 490 million tons in 2018 and projected to reach 550 million tons by 2030 and 590 million tons by 2040. Rice is thinly traded in international market because it is a highly protected commodity. Only about 9% of the total production is traded in global rice market. However, the volume of global rice trade has increased over six-fold from 7.5 to 46.5 million tons between the 1960s and 2018. A relatively small number of exporting countries interact with a large number of importing countries. The top five rice exporting countries are India, Thailand, Vietnam, Pakistan, and China accounting for 74% of the global rice export. The top five rice importing countries are China, Philippines, Nigeria, European Union and Saudi Arabia accounting for 26% of the global rice import. Within rice varieties, Japonica rice accounts for the highest share of the global rice trade (about 12%) followed by Basmati rice (about 10%). The high concentration of exports to a few countries makes international rice market vulnerable to supply disruptions in exporting countries, leading to higher world prices of rice. The export price of Thai 5% broken rice increased from 198 US$/ton in 2000 to 421 US$/ton in 2018. The volumes of trade and rice prices in the global market are expected to increase in the future. The major future challenges of the rice industry are increasing demand due to population growth, rising demand in Africa, economic growth and diet diversification, competition for natural resources (land and water), labor scarcity, climate change and natural hazards, poverty and inequality, hunger and malnutrition, urbanization, low income in rice farming, yield saturation, aging of farmers, feminization of agriculture, health and environmental concerns, improving value chains, and shifting donor priorities away from agriculture. At the same time, new opportunities are available due to access to new technologies, increased investment by the private sector, and increased global partnership. More investment in rice research and development is needed to develop and disseminate innovative technologies and practices to overcome problems and ensure food and nutrition security of the future population.

• International Journal of Industrial Entomology and Biomaterials
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• 제5권2호
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• pp.153-162
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• 2002

Earlier breeding experiments undertaken at Central Sericultural Research and Training Institute, Mysore, India since a decade had resulted in the development of many productive and qualitatively superior bivoltine hybrids. However, the hot climatic conditions of tropics prevailing particularly in summer are not conducive to rear these high yielding bivoltine hybrids. This has necessitated breeding of compatible bivoltine hybrids for year-round rearing. Accordingly, the Japanese hybrid, B2Ol ${\times}$ BCS12 which was found to be tolerant to high temperature was used as breeding resource material. Following hybridization and selection rearing of silkworms was taken up in SERICATRON (Environmental chamber with precise and automatic control facilities for uniform maintenance of temperature and humidity) at high temperature of $36{\times}1^{\circ}C$ and 85${\times}$5% RH in fifth instar and the control batches at $25{\times}1^{\circ}C$ and 65{\times}$5% RH. Directional selection was resorted to the batches reared at 36$\pm$1$^{\circ}C$ till F$_{5}$ keeping pupation rate as important selection criteria. From $F_{2}% onwards the experiment was modified in such a way as to conduct normal rearing every alternate generation to regain the lost vitality due to continuous exposure to high temperature and high humidity stress. At $F_{2}$ , Oval and dumb-bell cocoons were separated out and designated as CSR18 and CSR19, respectively. By utilizing these lines at $F_{12}$, the hybrid CSR18$\times$CSR19 was prepared and studied for the thermotolerance by subjecting to stress condition at high temperature of 36$\pm$1$^{\circ}C$ and 85$\pm$5% RH in fifth instar and the control batches at $25{\times}1^{\circ}C$ and 65${\times}$5% RH. The better performance of CSR18${\times}$CSR19 (survival > 88%) at $36{\times}1^{\circ}C$ clearly indicates the general superiority of CSR18${\times}$CSR19 with regard to high temperature tolerance over the productive hybrids and CSR18$\times$CSR19 can perform well in varied agro-climatic conditions of the tropics with optimum qualitative and quantitative characteristics.s.

• 자원ㆍ환경경제연구
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• 제18권3호
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• pp.457-493
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• 2009

본 연구는 기후변화 관련 Post-Kyoto 협상의 주요 쟁점사항들을 정리하고, 연산일반균형모형인 GTEM-KOR를 활용하여 경제적 분석을 실시하였다. 협상에서 해결해야 될 쟁점사항으로서 미국의 감축의무 참여, 개도국의 감축의무 참여, 광범위한 감축의무 참여 유도를 위한 감축의무 방식 개발 등을 제시하였으며, 이를 해결하기 위해 개도국간 차별화와 감축의무의 형태 및 강도 등이 중요한 협상 이슈가 될 것임을 지적하였다. GTEM-KOR를 활용한 분석에서는 일인당 GDP, 일인당 배출량 등 일인당 지수에 기초한 개도국간 차별화는 전 세계 온실가스 감축효과 측면에서 비효율적이며, 한국을 타 개도국에 비해 상대적으로 강한 감축의무 참여 압력에 노출시킬 것으로 분석되었다. 또한 기존 선진국뿐만 아니라 중국, 인도 등 주요 개도국의 감축의무 참여가 Post-Kyoto 체제의 온실가스 감축효과를 결정하는 중요한 요소임을 확인하였다. 감축의무 참여범위가 확대될수록 감축효과는 커지고 한계저감비용은 절감될 것으로 분석되었다. 한편 감축의무의 형태보다는 감축의무의 상대적 강도와 국가들의 참여범위가 Post-Kyoto 체제의 경제 및 환경적 효율성을 결정하는 중요한 요소임을 확인하였다.

• 한국응용곤충학회지
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• 제22권2호
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• pp.52-66
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• 1983

The brown planthopper, N. lugens (Stal), has become a serious pest of rice in tropical Asia during the last decade. At high pest density, its feeding damage causes 'hopperburn' or complete wilting and drying of the rice plant. It also transmits grassy and ragged stunt virus diseases. The estimated losses caused by the pest in tropical Asia exceed $US\$300$ millions. While cultivation of resistant rice varieties has proved to be highly effective against the pest, their long-term stability is threatened because of the evolution of prolific biotypes which can destroy these varieties. At present, identification of biotypes is based principally on the differential reactions of host rice varieties to the pest and on host-mediated behavioral and physiological responses of the pest. Recent findings of morphological differences in adult rostrum, legs, and antennae, body parts that possess receptors for host plant location and discrimination, and cytological differences in N. lugens populations maintained as stock cultures strongly complement other biotype studies. So far, three N. lugens biotypes have been identified in the Philippines. Biotype I can survive on and damage varieties that do not carry and genes for resistance, while Biotype 2 survives on resistant varieties carrying Bph 1 gene and Biotype 3 on varieties carrying gene bph 2. However, none of these biotypes can survive on varieties with genes Bph 3 or bph 4. Several varieties which are resistant in the Philippines are susceptible in India and Sri Lanka as the South Asian biotypes of N. lugens are more virulent than Southeast Asian biotypes. To monitor the pest biotypes in different geographical regions and to identify new sources of resistance, an International Brown Planthopper Nursery has been established in many cooperating countries. The evolution of biotypes is an exceedingly complex process which is governed by the interactions of genetic and biological factors of the pest populations and the genetic makeup of the cultivated varieties. While the strategy for sequential release of varieties with major resistance genes has been fairly successful so far, the monegenic resistance of these varieties makes them vulnerable to the development of the pest biotypes. Therefore, present breeding endeavors envisage utilizing both major and minor resistance genes for effective control of the pest.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.17-22
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• 2008

Aromatic rice has become popular owing to its aroma. Growing demand for aromatic rice has spurred interest in the development of domestic cultivars that offer similar combinations of grain attributes such as texture, cooking characteristics, aroma, and taste. In this study, the most important agronomic attributes and aroma of 26 cultivars from Afghanistan, Iran, and Uzbekistan, and controls from Japan, Thailand, and India were characterized. Also $F_2$ populations derived from the cross between(Jasmine 85 aromatic$\times$Nipponbare non-aromatic) and(Jasmine 85$\times$Basmati 370 aromatic) were obtained. Tasting individual grains, cooking test, 1.7% KOH sensory test, and molecular marker analysis have been applied to distinguish between aromatic and non-aromatic rice. Diversity for some traits of agronomic importance, such as plant height was detected among countries, e.g. Afghan cultivars classified as tall, and Iranian and Uzbek intermediate and short, respectively. Differentiations of panicle, grain, leaf, basal internode, and culm dimension among rice cultivars, indicating the source of rice diversity in Central Asia. According to the results, 6 of 10, 2 of 7, and 0 of 6 of Afghan, Iranian, and Uzbek rice cultivars were scored as aromatic, respectively. Therefore, Afghan cultivars are a good source of aromatic rice germplasm for Central Asia. The expression between aromatic and non-aromatic, and aromatic and aromatic combinations has been evaluated. The observed segregation ratio of these crosses in the $F_2$ populations was tested by $x^2$ analysis against the expected ratio for a single gene. A segregation ratio of 3:1 between non-aromatic and aromatic combination has been detected, while segregation has not been detected between the aromatic and aromatic combinations. Also, parallel results were obtained from the tested aromatic rice cultivars. Thus, our results suggest that a single recessive gene controls aroma in all aromatic rice cultivars.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2629-2633
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• 2012

Background: Raw betel nut (RBN) chewing is an important contributing factor for esophageal squamous cell carcinoma (ESCC), although associated genomic changes remain unclear. One difficulty in assessing the effects of exclusively RBN induced genetic alterations has been that earlier studies were performed with samples of patients commonly using tobacco and alcohol, in addition to betel-quid. Both CDKN2A (at 9p21) and Rb1 gene (at 13q14.2) are regarded as tumor suppressors involved in the development of ESCC. Therefore, the present study aimed to verify the RBN's ability to induce ESCC and assess the involvement of CDKN2A and Rb1 genes. Methods: A panel of dinucelotide polymorphic markers were chosen for loss of heterozygosity studies in 93 samples of which 34 were collected from patients with only RBN-chewing habit. Promoter hypermethylation was also investigated. Results: Loss in microsatellite markers D9S1748 and D9S1749, located close to exon $1{\beta}$ of CDKN2A/ARF gene at 9p21, was noted in 40% ESCC samples with the habit of RBN-chewing alone. Involvement of a novel site in the 9p23 region was also observed. Promoter hypermethylation of CDKN2A gene in the samples with the habit of only RBN-chewing alone was significantly higher (p=0.01) than Rb1 gene, also from the samples having the habit of use both RBN and tobacco (p=0.047). Conclusions: The data indicate that the disruption of 9p21 where CDKN2A gene resides, is the most frequent critical genetic event in RBN-associated carcinogenesis. The involvement of 9p23 as well as 13q14.2 could be required in later stages in RBN-mediated carcinogenesis.

• 한국콘텐츠학회논문지
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• 제14권6호
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• pp.34-43
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• 2014

중국 수용자들은 아이치이, 유쿠, 러스왕, PPTV 등 온라인 동영상 서비스 같은 미디어 환경의 변화로 여러 채널을 통해 한국, 미국, 일본, 영국, 태국, 인도 등 다양한 국가의 드라마, 영화, 오락 프로그램 등을 선택, 시청할 수가 있다. 최근 다양한 장르의 한국 대중문화가 중국에서 인기를 끌고 있는데, 이러한 현상은 중년 여성을 중심으로 한 드라마가 인기였던 초기 한류 시기(1997-200년대 초)와는 달리, 현재는 드라마뿐만 아니라 K-pop, 오락 프로그램 등 다양한 장르로 퍼져나갔고 인터넷 동영상 서비스를 통해 한국 대중문화를 접할 수 있게 되었다. 본 연구는 중국 80-90호우(后)의 외국 대중문화 수용태도를 파악하고자 한국, 미국, 일본 대중문화의 선호 장르와 이유, 선호 대중문화 및 국가 이미지를 비교분석하였다. 분석결과, 국가별 선호 장르와 이유에서의 유사점과 차이점을 발견할 수 있었으며, 선호 대중문화와 국가 이미지와의 밀접한 관계를 파악할 수 있었다. 본 연구의 결과를 통해 중국에서의 한류의 지속가능한 발전 모색을 위한 기초자료로 사용하는데 기여하고자 한다.