• Journal of Microbiology and Biotechnology
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• 제22권9호
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• pp.1271-1278
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• 2012

Genetic fusion of two proteins frequently induces beneficial effects to the proteins, such as increased solubility, besides the combination of two protein functions. Here, we study the effects of the bacterial surface layer protein SgsE from Geobacillus stearothermophilus NRS 2004/3a on the folding of a C-terminally fused enhanced green fluorescent protein (EGFP) moiety. Although GFPs are generally unable to adopt a functional confirmation in the bacterial periplasm of Escherichia coli cells, we observed periplasmic fluorescence from a chimera of a 150-amino-acid N-terminal truncation of SgsE and EGFP. Based on this finding, unfolding and refolding kinetics of different S-layer-EGFP chimeras, a maltose binding protein-EGFP chimera, and sole EGFP were monitored using green fluorescence as indicator for the folded protein state. Calculated apparent rate constants for unfolding and refolding indicated different folding pathways for EGFP depending on the fusion partner used, and a clearly stabilizing effect was observed for the SgsE_C fusion moiety. Thermal stability, as determined by differential scanning calorimetry, and unfolding equilibria were found to be independent of the fused partner. We conclude that the stabilizing effect SgsE_C exerts on EGFP is due to a reduction of degrees of freedom for folding of EGFP in the fused state.

• 한국콘텐츠학회논문지
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• 제18권3호
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• pp.499-508
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• 2018

이 연구는 경찰공무원을 대상으로 우리나라에서 최초로 회복탄력성을 측정하고, 조직몰입에 미치는 영향을 검증하여 경찰조직을 대상으로 한 실증적 연구의 방향성을 제시하는데 목적이 있다. 회복탄력성에 대한 선행연구의 분석을 통해서 회복탄력성의 발전과정과 하위 개념들을 정리하고, 경찰공무원의 회복탄력성이 조직몰입과 어떤 관계가 있는지를 분석하고자 하였다. 따라서 회복탄력성의 하위 개념을 자기조절능력(감정조절력, 원인분석력, 충동통제력), 대인관계능력(자아확장력, 소통능력, 공감능력), 긍정성(감사하기, 생활만족, 낙관성)으로 나누어 독립변수로, 조직몰입(감정몰입, 지속몰입, 규범몰입)을 종속변수로 사용하여 분석모형을 설정하였다. 분석결과 회복탄력성이 조직몰입에 미치는 영향을 생활만족>원인분석력>소통능력>공감능력>감사하기>충동통제력 순으로 모두 감정몰입에 정(+)적인 영향을 미치고, 생활만족>낙관성>감정조절력>소통능력>원인분석력 순으로 지속몰입에 정(+)적인 영향을 미치며, 자아확장력>공감능력>낙관성>생활만족>충동통제력>원인분석력 순으로 모두 규범몰입에 정(+)적인 영향을 미치는 것으로 나타났다.

• 한국도서관정보학회지
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• 제46권1호
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• pp.321-344
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• 2015

이 논문은 여러가지 자료를 결합해 어떤 주제에 관한 의견이 실려있는 블로그를 찾는 융합적 의견탐지방법을 소개한다. 주제에 관한 의견이 담긴 블로그를 찾기위해 이 연구는 기존의 IR 방법으로 주제에 관한 블로그를 검색한 후 여러가지 의견탐지 방법을 합산한 의견점수로 검색결과의 순위를 조정하는 방법을 쓴다. 의견탐지 모듈의 주요 구성 요소는 의견이 실려있는 블로그에 자주 나오는 단어들을 활용한 고빈도 모듈, 강한 감정을 표현하는 희귀 한 용어들을 (e.g., "sooo good") 활용한 저빈도 모듈, "I"와 "you"에 묶인 n-gram을 (e.g., I believe, You will love) 활용한 IU모듈, 윌슨의 주관 용어 목록을 바탕으로 한 윌슨의 어휘모듈, 그리고 소수의 의견 약어를 (e.g., imho) 이용한 의견 약어 모듈들 이다. 본 연구의 결과는 여러 가지 방법을 융합하는 것이 의견 검출 성능을 향상시키는데 효과적이 다는 것을 보여주었다.

• 치위생과학회지
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• 제20권3호
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• pp.126-135
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• 2020

Background: The purpose of this study is to investigate the effects of entrance exam stress on oral health behaviors and subjective oral health status in female high school students. Methods: A self-reported questionnaire was administered to 216 female high school students in the Gwangju area. We performed an independent t-test, one-way analysis of variance (ANOVA), Pearson's correlation coefficient, and multiple regression analysis using the SPSS/WIN 21.0 program. Results: The average of the entrance exam stress was 2.73, and among the sub-areas, the tension for exam/poor stress was the highest with 3.21. As a result of analysis of general characteristics and subjective oral health status, oral mucosal disease was lower in family income level (p<0.05), bad breath was significantly higher with lower academic performance (p<0.001) and family income level (p<0.05). As a result of oral health behavior and subjective oral health status analysis, dental caries has more than 4 times of caries snacks (per week) (p<0.05), bad breath was found to be more perceived when the average number of daily brushing was 2 or less (p<0.01). Factors affecting subjective oral health status were that dental caries was a patients pressure stress (β=0.202); temporomandibular disorder (β=0.227), xerostomia (β=0.342), and oral mucosal disease (β=0.190) were insufficient spare time; bad breath was academic performance (lower) (β=0.231) and insufficient spare time (β=0.184). There was a statistically significant positive correlation between the subjective oral health status and the subarea of entrance exam stress, excluding future uncertainty stress. Conclusion: It is considered that oral health education should be conducted to prevent oral diseases for students with high parental pressure and insufficient spare time stress as well as finding practical ways to reduce entrance stress.

• 대한가정학회지
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• 제26권1호
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• pp.21-31
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• 1988

The main problem of consumer behavior is choice since the outcome of me of a choice can only be known in the future, consumers are forced to deal with the risks of und\certainty. So, perception of risk is pivotal aspect of consumer behavior. This study was designed to investigate the relationships between purchasing behavior of textile and perceived risk. Data were obtained from 276 housewives. ANOVA, x2-test were employed to analyse the data. The result were : 1. general features of textile purchase behavior are as follows. Blend wools and pure wool products are prefered. Fall is the major season in purchasing textile. Wholesalers, department stores and agent stores are prefered. Purchasing decision making process independent upon not only textile itself but the practice value of the textile. Purchasing textile, married young women depend on outward shape of the textile, middle and old aged groups depend on the economic value of the textile and the credibility of the stores. 2. Perception of risk is relatively high in the preference of store, color/design, and psycological uncertainty. But the perceived risks is relatively low in brand, price and social credibility. 3. There is significant relationship between the recognition rate of risk and the sensitiveness of the consumer. In addition, there are strong relationship between the risk rate and the preference of shop, brand, and price. On the other hand, there are no significant relationship between the color, design, and sociopsyco-logical risk and demographic variables. 4. The perceived risk of consumer would be a key stone to grasp the consumer behavior. The product company needs to provide full information which could reduce the perceived risk of consumer. there attitude would help for the mutual interests. In the future research, we need to develop the precise methods for finding variables on the perceived risk during the process of making purchase intention.

• Journal of Gastric Cancer
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• 제4권3호
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• pp.164-168
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• 2004

Purpose: The most feared complication of gastrointestinal tract operations is anastomotic leakage, not only because of the presumed individual surgeon's culpability but also because of the assumption that this event is often fatal. We have experienced 32 cases of anastomotic leakage after elective gastric resection during 8 years. The purpose of this study was to evaluate the result of their treatment. Materials and Methods: We evaluated the records of 1335 patients who had undergone elective gastric resection for an adenocarcinoma of stomach from January 1995 to October 2003 and conducted a retrospective, multivariate analysis. Results: Of the 1335 patients, 32 ($2.4\%$) sustained an anastomotic leakage. Anastomotic leakages usually developed on mean postoperative day $9.1\pm3.2$ (range:$1\∼18$ days).Overall, $31.3\%$ (10/32) of patients who sustained an anastomotic leakage died. The anastomotic leakages were identifed by radiological study or by operative finding at the site of the duodenal stump (20 patients), the esophagojejunostomy (7), the gastroduodenostomy (4), and the gastrojejunostomy (1). Fourteen patients ($43.8\%$) underwent a relaparotomy, a drainage procedure in the main, and 18 patients ($56.3\%$) were treated conservatively. The mortality rates were $42.9\%$ (6/14) and $22.2\%$ (4/18), respectively, but this difference was not statistically significant. A cox's proportional hazard analysis showed that a body-mass Index < 24 kg/m2 (odds ratio 5.55, $95\%$ CI: $0.69\∼44.82$) and non-enteral feeding (odds ratio 18.27, $95\%$ CI 2.22.150.69) were independent factors of mortality due to anastomotic leakage. Conclusion: Our observations show that anastomotic leakage after an elective gastric resection has a high risk of being fatal. Moreover, for a patient with a body-mass index lower than $24\;kg/m^{2}$ and/or non-enteral feeding, an anastomotic leakage after an elective gastric resection has a higher risk of being fatal.

• 한국병원경영학회지
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• 제20권4호
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• pp.14-30
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• 2015

Purpose: This study was conducted to investigate the independent role of sub-dimensions of job stress, DiSC(R) type of personal behavior, and organizational social capital on job satisfaction and to identify the structural relation among them. Method: Study subjects were 317 registrated nurses employed in 4 general hospitals in a metropolitan city. Results: As the results of multiple regression analysis, the factors influenced independently on overall job satisfaction were as follows; job stress were significantly decreased job satisfaction. Regarding DiSC, job satisfaction of influence type was significantly higher than that of conscientiousness type. Of sub-dimension of OCS, the higher common value and reciprocity were, the high over all job satisfaction, but in a sub-dimension(trust), the relationship was reversed. Major findings of structural equation model analysis were as follows. Regarding DiSC, there were founded only direct effect on job satisfaction(D, i, S>C in relations with peer and others, job performance, retrospectively). Regarding common value of OCS, there were founded significant positive direct effect and indirect effect via job stress on all sub-dimensions of job satisfaction. Regarding trust of OCS, there were founded negative direct effect alone on 2 sub-dimensions of job satisfaction(work itself, job performance). Regarding trust of OCS, there were founded positive direct and indirect effect on satisfaction towards work itself, and indirect effect alone on 2 sub-dimensions(relations with peer and others, job performance). Conclusion: Summing up above finding, to manage job satisfaction of nurses, it is suggested for nursing staffs to provide behavioral training programs according to type of DiSC(R) and to introduce strategic programs fostering organizational social capital such as common vision and reciprocity.

• 한국전문물리치료학회지
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• 제27권4호
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• pp.241-249
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• 2020

Background: Prolonged standing during work causes a lower extremity pain and disorders. Patellofemoral pain syndrome (PFPS) is one of the common diagnoses of the knee pain. Although the etiology of PFPS is not completely understood, it is considered to be multifactorial. Objects: The purpose of this study was to investigate difference in strength of knee muscles, quadriceps:hamstring muscles strength ratio (Q:H ratio), asymmetry ratio of knee muscles strength and dorsiflexion range of motion (ROM) between standing workers with and without PFPS. Methods: Twenty-eight standing workers with PFPS and 26 age-, height-, and weight-matched standing workers without PFPS participated in this study. A tension sensor measured knee muscle strength, and motion sensor measured dorsiflexion ROM. The asymmetry ratio of knee muscles was calculated by a specific formula using the knee muscles strength of the dominant side and the sound side. An independent t-test was used to identify significant differences in the strength, ROM, Q:H ratio, and asymmetry ratio between the PFPS and normal groups. Results: The standing worker with PFPS have significantly lower dorsiflexion ROM (p < 0.000) and higher asymmetry ratio of the hamstring muscles strength (p < 0.000) compare to the standing worker without PFPS. No significant differences were seen in the strength of quadriceps muscle and hamstring muscles, Q:H ratio, and asymmetry ratio of quadriceps muscle strength. Conclusion: There was a significant difference in the asymmetry ratio of the isometric hamstring muscle strength. This finding suggests that the asymmetry ratio of isometric hamstring muscle strength may be more important than measuring only the hamstring muscle strength of the PFPS side. Furthermore, the results of this study showed a significant difference in dorsiflexion ROM between the standing industrial workers with and without PFPS. Dorsiflexion ROM and isometric hamstring muscle strength should be considered when evaluating the subjects with PFPS.

• 한국소음진동공학회논문집
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• 제15권10호
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• pp.1177-1185
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• 2005

This paper investigates the noise radiated by a cascade of flat-plate airfoils interacting with homogeneous, isotropic turbulence. At frequencies above the critical frequency, all wavenumber components of turbulence excite propagating cascade modes, and cascade effects are shown to be relatively weak. In this frequency range, acoustic power was shown to be approximately proportional to the number of blades. Based on this finding at high frequencies, an approximate expression is derived for the power spectrum that is valid above the critical frequency and which is in excellent agreement with the exact expression for the broadband power spectrum. The approximate expression shows explicitly that the acoustic Power above the critical frequency is proportional to the blade number, independent of the solidity, and varies with frequency as ${\phi}_{ww}(\omega/W$), where ${\phi}_{ww}$ is the wavenumber spectrum of the turbulence velocity and W is mean-flow speed. The formulation is used to perform a parametric study on the effects on the power spectrum of the blade number stagger angle, gap-chord ratio and Mach number. The theory is also shown to provide a close fit to the measured spectrum of rotor-stator interaction when the mean square turbulence velocity and length-scale are chosen appropriately.

• 한국치위생학회지
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• 제13권6호
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• pp.1087-1094
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• 2013

Objectives : In this study, for before/after dental implant surgery, our aim is to provide the basic data based on the decision of the treatment by measuring satisfaction of the treatment and finding out the intention to revisit a hospital and also by recommendation. Methods : We conducted the frequency analysis, a cross-tabulations, paired t-test and a correlation analysis of 146 data who had visited at 6 dental clinics and hospitals located in Daegu for dental implant surgery, with SPSS (PASW 18.0 for Windows, SPSS Inc, USA) to find out the satisfaction of the treatment before/after dental implant surgery. Results : The satisfaction related to before/after dental implant surgery of oral health was all statistically significant on a masticatory, social and psychological function, the satisfaction towards the treatment, the intention to revisit a hospital and also by recommendation. Independent variables explained the satisfaction of the treatment before/after dental implant surgery as 19.1% and indicated a significantly high value in general. Conclusions : The satisfaction was higher on satisfactory of the postoperative treatment than on satisfactory of the preoperative treatment in spite of these limitations. Therefore, the dental medical team should take into account dental implanting as a way which improves the satisfaction of the treatment related with oral health and have continuous managements and careful concerns.