• 제목/요약/키워드: Incubating

검색결과 356건 처리시간 0.021초

Immobilized Biocatalysts를 이용한 환경성 폐기물질 억제에 관한 연구 (제3보) 알코올 발효를 위한 Immobilized Biocatalysts 제조 (Studies on the Control of Environmental Wastes by Means of Immobilized Biocatalysts (III) Preparation of Immobilized Biocatalyst to Ethanol Fermentation)

  • 김성기
    • 한국환경보건학회지
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    • 제17권1호
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    • pp.120-128
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    • 1991
  • Saccharomyces cerevisiae was immobilized by incubating iron oxides with calcium alginate, and by polyacrylamide entrapment to use repeatedly for the conversion of glucose to ethanol. Magnetic and non-magnetic immobilized yeast and polyacrylamide immobilized yeast were compared with the native yeast a batch-fermentation of ethanol from glucose. Three kinds of immobilized yeast tended almost identically, having ethanol productivity as well as the final yield about the same to what was found for the native yeast. The long-term operational stability of three kinds of immobilized yeast were significant difference according as immobilized yeast activation or non-activation before ethanol fermentation. In the non-activation they lost their activity of fermentation rapidly in the beginning stage an slower at a later stage. On the other hand, in the activation with nutrient media, their activities were increased to some extent and stable in the later stage. The cell count of three kinds of immobilized yeast after activiation by incubating nutrient media, increased by a factor of about 45 to 48, whereas the fermenting capacity increased by a factor of 174 to 178. In the prearation of immobilized biocatalysts, magnetic matter does not seem to have any adverse affect on the properties of the microorganism. The immobilized biocatalysts by utilizing magnetic matter have some advantages, especially in application of viscous media or insoluble particle-containing media, for this work was linked with microbial utilization of environmental wastes and elimination of envirnmental pollutant.

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Recombinant Interferon-${\alpha}$ Cross-linked with Thymosin ${\alpha}$1 is Biologically Active

  • Jeong, Jee-Yeong;Chung, Hye-Shin
    • BMB Reports
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    • 제29권4호
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    • pp.365-371
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    • 1996
  • Partially reduced interferon-a ($IFN-{\alpha}$) was cross-linked with thymosin ${\alpha}1$ ($T{\alpha}1$) using sulfo-succinimidyl (4-iodoacetyl) amino benzoate (SIAB), a bifunctional cross-linking reagent. The partially reduced $IFN-{\alpha}$ optimal for the cross-linking reaction was obtained by incubating native $IFN-{\alpha}$ with 0.5 mM DTT at $30^{\circ}C$ for 60~100 min. $T{\alpha}1$ was activated by incubating with sulfo-SIAB at $37^{\circ}C$ for 30 min to produce $T{\alpha}1-IAB$. The $T{\alpha}1-IFN-{\alpha}$ cross-linking was achieved by the reaction of the partially reduced $IFN-{\alpha}$ with $T{\alpha}1-IAB$. This cross-linking was between the sulfhydryl group of Cys1 in $IFN-{\alpha}$ and the N-terminal amino group of $T{\alpha}1$ through acetyl amino benzoate as a spacer. The immunological activity of the cross-linked molecule showed the same extent as that of $T{\alpha}1$, and most of the antiviral activity was retained compared to that of the partially reduced $IFN-{\alpha}$.

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생강 추출물의 항산화 효과에 관한 연구 (Antioxidative Effect of Ginger Extracts)

  • 김은정;안명수
    • 한국식품조리과학회지
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    • 제9권1호
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    • pp.37-42
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    • 1993
  • 이상의 결과에서 다음과 같은 생강 추출물의 항산화 효과를 요약할 수 있었다. 1. 항온 저장시에는 생강 추출물을 첨가한 모든 시료에서 항산화 효과를 나타내었으며 그 효과는 3%를 첨가시 가장 높았고 그 정도는 BHT와는 비슷한 효과를 보였으나 tocopherol에 비해서는 월등히 높은 항산화 효과를 나타내었다. 2. 가열 처리시에도 생강 추출물의 농도에 관계없이 비슷한 정도의 항산화 효과를 나타내었으며 특히 대두유의 경우에는 BHT, tocopherol보다 뚜렷하게 높은 항산화 효과를 보였다. 3. 건강편을 튀김유에 5 및 10%씩 넣고 가열한 후 튀김 횟수에 따른 튀김유의 산패 정도를 측정시 두 시료 모두에서 산패가 억제되었으며 그 중 5%의 건강편이 더 우수한 것으로 나타났다.

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Heparin이 소 정자의 수정능획득반응에 미치는 영향에 관한 연구 (Study on Effect of Heparin on Bovine Sperm Capacitation)

  • 박영식;임경순
    • 한국가축번식학회지
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    • 제14권4호
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    • pp.303-308
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    • 1990
  • To investigate the effect of heparin on sperm capacitation. The acrosome reaction of bovine sperm which were incubated in mTALP containing heparin and the in vitro development of bovine follicular oocytes which were cocultured with heparin-treated sperm were evaluated, and the resutls were as follows : 1. When bovine fresh sperm were incubated in mTALP solution containing 0, 5, 10 and 25$\mu\textrm{g}$/ml heparin for 15 to 840 minutes, there was no significant difference between motilies of heparin-treated sperm and untreated sperm, but the acrosome-reaction rate of heparin-treated sperm was significantly higher than that of untreated sperm. Moreover the acrosome reaction rate of was sperm treated with heparin was significantly increased after incubating for 15 minutes. 2. When fresh sperm were incubated in mTALP solution containing 10$\mu\textrm{g}$/ml heparn for 840 minutes, the motility of sper incubated for 840 minutes was lower than those of sperm incubated for 0, 15, 60 and 120 minutes, but the acrosome-reaction rate of sperm incubated for 840 minutes was higher than those of the others. 3. When frozen-sperm were incubated in mTALP solution containing 10$\mu\textrm{g}$/ml heparin for 120 minutes, the acrosome reaction rate of sperm was significantly increased after incubating for 15 minutes. 4. When fresh sperm treated with heparin were cocultured with bovine follicular oocytes, 16.7 to 23.7% of the oocytes were developed to 2-8 cell stage.

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기술 및 경영지원 전담제도 도입에 의한 창업보육 육성방안 (Incubating Consultant in Business Incubator)

  • 허용정
    • 한국산학기술학회:학술대회논문집
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    • 한국산학기술학회 2002년도 춘계학술발표논문집
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    • pp.81-83
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    • 2002
  • 한국기술교육대학교(이하 한기대) 창업보육센터에서는 대학의 산학협동 정책의 일환인 교수 현장연구학기제, 중소기업기술애로지도 둥의 제도와 연계를 토대로 입주업체의 기술 및 경영 지원을 실시하고 있다 특히 보육닥터제도란 대학의 고급인력(교수, 대학원생)을 활용하여 창업보육센터에 입주한 업체에 대한 기술지도 및 경영지도를 실시하는 것을 말하며, 보육사업을 효율적으로 지원하는데 그 목적이 있다 하겠다. 대학이 갖춘 양질의 고급기술인력과 제도를 적절히 활용한다면 향후 그 효과 및 성과가 기대되며, 또한 보육닥터제의 운영방안과 방법들에 대하여 고찰하여 발전방향을 모색해 보고자 한다.

부화계태아의 수분대사에 관한 연구 - II. 부화일령에 따른 계태아의 각조직 및 기관에 대한 수분분포 (Studies on Water Metabolism in Developing Chick Embryos - II. Water Distribution to Different Tissues and Organs of Developing Chick Embryos in Various Incubating Stage)

  • 김영홍
    • 대한수의사회지
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    • 제16권4_5호
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    • pp.193-198
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    • 1980
  • The studies were under taken with the objective to observe water distribution and its content of developing embryo by dry method during the incubation periods. The results obtained were summarized as follows: 1. For the first time each developing embryoni

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부화계태아의 수분대사에 관한 연구 - I. 부화일령에 따른 계태야의 수분분할 (Studies on Water Metabolism in Developing Chick Embryos - I. Water Division of Developing Chick Embryos in Various Incubating Stages)

  • 김영홍
    • 대한수의사회지
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    • 제16권4_5호
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    • pp.189-192
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    • 1980
  • The studies were undertaken with the objective to observe water distribution and its content in egg during the incubation periods by dry method. The resalts obtained were sammarized as follows: 1. Chick embryo was not recogenizable on second and third day

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IT 벤처기업의 애로요인과 벤처인큐베이팅 시스템 (The Development Bottlenecks of IT Venture Companies and Venture Incubating System)

  • 박범수;김서균;박태웅
    • 전자통신동향분석
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    • 제17권4호통권76호
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    • pp.113-122
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    • 2002
  • 급속한 기술혁신과 변화 속에서 IT 벤처기업은 국가의 경제성장과 발전을 이끄는 원동력으로 작용하고 있다. 정부의 정책적 지원 하에서 벤처기업은 양적으로도 엄청난 규모로 성장하였다. 그럼에도 창업초기에 있는 벤처기업들은 예전과 마찬가지로 그들이 직면한 시장의 비효율성으로 인해 쉽게 어려움에서 벗어나지 못하고 있다. 본 연구는 창업초기 IT 벤처기업들이 겪고 있는 애로요인들을 시장 효율성과 시스템적 관점에서 접근함으로써 IT 벤처기업의 질적 성장을 위한 정책적 해결점을 찾아 보고자 한다.