• Title/Summary/Keyword: Incompatibility

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Molecular Characteristics of R Plasmids in Shigella (Shigella R Plasmid의 분자적 특성)

  • Lee, Yoo-Chul;Seol, Sung-Yong;Cho, Dong-Taek;Chun, Do-Ki
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.1
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    • pp.35-53
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    • 1987
  • Multiply resistant Shigella strains isolated in Taegu area were subjected for the characterization of R plasmids. All strains isolated in 1984 and 1985 were susceptible to gentamicin, amikacin, and cephalothin, and most strains were susceptible to kanamycin (Km) and rifampin by agar dilution antimicrobial susceptibility test. The resistance frequency of S. flexneri against ampicillin (Ap) was higher than that of S. sonnei. The strains resistant to sulfisomidine (Su) and trimethoprim (Tp) were found at higher frequency in S. sonnei than in S. flexneri. The most prevalent resistance pattern of S. flexneri was chloramphenicol (Cm) tetracycline (Tc) streptomycin (Sm) Ap, followed by the pattern of CmTcSmSuApTp, CmTcSmSuApTp nalidixic acid, and CmTcSmSuAp in the decreasing order. The antibiogram of CmTcSmSuTp was found to be the most frequent pattern in S. sonnei. The ratio of conjugal transfer of S. flexneri was 47% and 75% of S. sonnei. The average number of plasmid harboring in Shigella was 4 and the size of plasmid ranged 1.3 to 134 megadalton (Mdal). Most S. flexneri carried plasmids of 2 to 3 Mdal and S. sonnei carried those of 3 to 4 Mdal size. The sizes of conjugative plasmids ranged 40-90 Mdal. The incompatibility group (Inc) F II plasmids (54-59 Mdal) were most frequent and rare Inc B plasmids (60 Mdal) of isolates in 1979 and 1980 and Inc FI (87 Mdal) of 1983 isolates were able to be classified by the colony test with standard reference plasmids. The R plasmids of known Inc group were tested for the restriction endonuclease analysis. The pattern of plasmids digested by EcoRl were apparently different by the Inc group but there was no significant difference between species or by the resistance patterns. Nonconjugative plasmids and their phenotypes were identified by transformation test. The transformants were resistant to less than two drugs. Colicin producing transformants carried the Col plasmid of 3.7 or 3.9 Mdal size. $Ap^r$ plasmids derived from S. sonnei were found to be mobilized by transfer factor RT641 to E. coli #CS100. $Ap^r$ plasm ids of same size shared by S. flexneri, S. sonnei, and E. coli were digested with Pstl. All of them showed two restriction fragments of 2.8 kilobase(kb) and 0.7kb. Other plasmids ($Sm^r\;Su^r$) derived from S. flexneri, S. boydii, and S. sonnei were digested with Pstl and they showed same restriction fragment patterns of 3.1kb and 2.9kb. The plasmid profiles of three strains of S. sonnei producing colicin and showing same resistance pattern of CmTcSmSuApTpKm appeared to be similar. Restriction patterns by EcoRl and the behavior of plasmids in conjugation or transformation process were also similar between those plasmids. The restriction patterns were significantly different between the plasmids of Inc FI group and those of unclassified Inc group.

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Pollination Methods for Overcoming Pre-fertilization Incompatibility in Interspecific Crosses between Lilium longiflorum 'Gelria' and L. cernuum Native in Korea (나팔나리와 자생 솔나리와의 종간 교잡 시 수정 전 불화합성 타파를 위한 수분방법)

  • Kim, Young Jin;Park, Sung Min;Kim, Jong Hwa
    • Horticultural Science & Technology
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    • v.19 no.3
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    • pp.373-377
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    • 2001
  • This study was conducted to find optimum pollination methods for overcoming pre-fertilization incompatibility in interspecific crosses between L. longiflorum 'Gelria' (LG) and L. cernuum (LC). In cross combination of $LC{\times}LG$, the survival rate of the ovaries was higher in the cut style pollinations than in normal stigmatic pollination. Among the various cut style pollination methods, pollination after cutting the styles 2 3mm length above the ovary and top of the ovary were the most effective ones. No ovaries and ovules survived in cross pollination of $LC{\times}LG$. In self-pollination of LG and cross pollination of $LC{\times}LG$, pollens germinated on the stigma by 12 hours and reached to the ovules by 90 hours after pollination. However, in the cross pollination of $LC{\times}LG$, pollen tubes elongated very slowly until 48 hours after pollination and a lot of deformed tubes were observed in the styler canal having swelled tube tip and irregular heavy callose deposition. The pollination time after anthesis also affected on the survival rate of ovaries and ovules showing the best result when pollinated on three days after anthesis. Stigma exudate promoted slightly the enlargement of ovaries, but the survival rate of ovaries and ovules was not increased.

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Study on Flowering, Pollination and Samara Characteristics of Chinese elm, Ulmus parvifolia in Wonju, Korea (참느릅나무의 개화, 수분 및 결실 특성에 관한 연구)

  • Kim, Gab-Tae;Kim, Hoi-Jin;Choo, Gab-Cheul
    • Korean Journal of Environment and Ecology
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    • v.26 no.4
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    • pp.588-592
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    • 2012
  • To examine the reason of empty samara production of Chinese elm, Ulmus parviflora, twenty two planted trees in Wonju-si were monitered for three years in terms of their flowering, pollinatiom system and samara characteristics. Inflorescences with bisexual flowers of Chinese elm are developed in the leaf axils on the twigs. Dichogamous flowers are varied with protogynuous and protandrous flower, and stamens in some bisexual flowers are developed in seperated time on a inflorescence or a tree. It is revealed newly that the flower of Chinese elm is out-crossed and partially insect(Apis mellifera) pollinated. The ratios of sound samara are significantly differed among years, the heighest values 65.5% were shown in 2009, lowered 42.9% in 2010, and the lowest 37.5% in 2011. This result might be affected by mean daily precipitation and number of rainy days during the flowering date, and lower temperature during the floral initiation stage, especially in 2011. These findings suggest that Chinese elm has self-incompatibility strategy and much pollination failure resulted in a production of much empty samaras. Further researches on the empty-seed production strategies and pollination system of major tree species might be needed.

Comparative Toxicity Studies of Ultra-Pure Ag, Au, Co, and Cu Nanoparticles Generated by Laser Ablation in Biocompatible Aqueous Solution

  • Kim, Yea-Seul;Kim, Kuk-Ki;Shin, Seon-Mi;Park, Seung-Min;Hah, Sang-Soo
    • Bulletin of the Korean Chemical Society
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    • v.33 no.10
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    • pp.3265-3268
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    • 2012
  • Nanoparticles (NPs) are increasingly used in consumer products, which have aroused many concerns and debates regarding their fate in biological systems from a point of their safety/toxicity. Although a number of studies on the biological effects of NPs have been published, these are often complicated by the possible toxicity of conventional NPs, caused by contamination with chemical precursors or additives during their synthesis and/or purification procedures. To explicitly understand the toxicity basis of NPs, it is necessary to directly address a main problem related to their intrinsic/inherent toxicity and/or incompatibility with biological objects. The present study is designed to take advantage of a novel laser-assisted method called laser ablation to generate Ag, Au, Co, and Cu NPs in biocompatible aqueous solution, and to evaluate the toxicity of the resulting ultra-pure NPs. Our results show that the ultra-pure NPs with nascent surfaces possess moderate cytotoxicity to human cells in a cell-dependent manner.

Enforcement of Arbitral Awards Incompatible with the Korean Procedural Framework

  • Lim, Sue Hyun
    • Journal of Arbitration Studies
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    • v.30 no.3
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    • pp.67-94
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    • 2020
  • This paper examines the current enforcement regime of Korea and provides an overview of the same with focus on the changes before and after the 2016 revision of the Korean Arbitration Act. It briefly studies the pro-arbitration bias of the New York Convention, as well as the Korean judiciary's stance on the enforcement of foreign arbitral awards. Some of the substantial issues discussed in the paper include the major procedural changes brought about by the 2016 amendment with respect to the enforcement of arbitral awards. The paper also discusses the rare instances where the Korean judiciary refused to recognize or enforce an arbitral award, and the reasoning behind the refusal. The paper discusses and analyzes four court judgments that reflect the Korean judiciary's position on the enforcement of foreign and domestic arbitral awards in Korea. It focuses on the NDS v. KT Skylife case, where the court of first instance refused the enforcement on grounds that the relief granted by the arbitral tribunal was not specific enough for enforcement. Ultimately, the appellate court, although agreeing on the specificity requirement, reversed the ruling and granted an enforcement judgment on grounds that the application for enforcement had the legal interest to request an enforcement judgment.

A Study on the System of Litigation and Ideal Dispute Resolution (소송제도와 이상적인 분쟁해결제도에 관한 연구 - 대법원의 상고법원 설치안을 중심으로 -)

  • SHIN, Han-Dong
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.68
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    • pp.43-63
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    • 2015
  • The number of final appeal(the rate of final appeal: 43%) has been on the increase every year over the past ten years in Korea. The number of final appeal cases given to a justice of the Korean Supreme Court amounts to nearly one everyday, which makes it vulnerable to faulty decisions. Reversal rate of final appeal is as low as 10% with most of the cases being dismissed and hence the percentage of people having trust in the judiciary is merely 27%. In this context, the Korean judiciary has announced its plan to set up a final appellate court in the Supreme Court. The establishment of final appellate court, however, is not only against the Constitution but also hardly seen in other nations. It would only overexpand the Supreme Court. Furthermore, the final appellate court would end up deteriorating into the court of fourth instance and impose extra burden on the government as well as on the disputing parties. Therefore, it is necessary to upgrade the quality of the court by increasing the number of judges in the lower court and let them focus on the fact finding process. Facilitating the ADR(Alternative Dispute Resolution) process such as arbitration would help improve the structure of the judiciary. The incompatibility among the four values of the dispute resolution process(equitability, truth, quickness and efficiency) calls for building comprehensive judicial system in which disputes are settled by choosing either jurisprudence or utility.

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Control of Phythophthora Blight of Pepper by grafting

  • Hong, S.T.;Lee, K.H.;Jeong, J.H.;Park, C.W;Kim, J.J.;Kim, H.T.
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.110.1-110
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    • 2003
  • Control effect of grafting with rootstocks on Phythophthora blight of pepper was evaluated. The 40∼45 day old seedlings of five pepper cultivars, Wangdaeback, Jinmi, Boosa, Samsungcho and Pochunngchun, as a scion were grafted with ten species of root stocks. There wasn't graft incompatibility in all scion-root stock combinations. After 21 days from inoculation with Phytopkhora capsici, incidence of Phythophthora blight of plant grafted with Kataguruma, R-Safe, R-16, YCM 334 and SCM 334 of rootstocks tested was decreased by 64.6∼100% compared to non-grafted plant whose disease severity was 3.7. However, plant grafted with Umsung native and Yeongdong native pepper was more sensitive to Phythophthora blight than non-grafed plant. Control effect by grafting was inversely proportional to virulence of inoculum and not significantly different among scion cultivars used. And resistant reaction of scion against Phytophthora blight was not affect that of scion-root stock plant in this study.

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Reconsideration of the Azimuth Functions in the Analysis of Heat Transfer by the Method of Similarity Transformations (상사변환법에 의한 열전달해석에 있어서 방위함수의 재고)

  • ;;Son, Byung Jin;Yi, Hyun
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.3 no.3
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    • pp.91-97
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    • 1979
  • Boundary layer equations (partial differential equations) can be transformed to ordinary diffential equations with constant coeffieients in terms of similarity transformed to ordinary differential equations with constant coeffieients in terms of similarity transformations in the heat tranfer analysis on the surface of any axiaymmetric boiles. The azimuth functions can not be uniquely determined because of the singular behavior at the stagnation point(X=0.deg.).In spite of the azimuth functions behaving singularly, many of researchers have analyzed the heat transfer problem on a horizontal chlinder or a sphere, supposing the set of solutions( $H_{1}$ & G$_{1}$) of being yieled from the simple differential equation to be unique solution of therazimuth functions. In order to ascertain whether mathematical incompatibility as mentioned above can be admitted in the viewpoint of enginerring or not, condensation heat transfer coefficients on a sphere are computed for all azimuth functions( $H_{1}$ G$_{1}$ & $H_{2}$ G$_{2}$) and comparisons with the experimental result are discussed.

A Tutorial: Information and Communications-based Intelligent Building Energy Monitoring and Efficient Systems

  • Seo, Si-O;Baek, Seung-Yong;Keum, Doyeop;Ryu, Seungwan;Cho, Choong-Ho
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.11
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    • pp.2676-2689
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    • 2013
  • Due to increased consumption of energy in the building environment, the building energy management systems (BEMS) solution has been developed to achieve energy saving and efficiency. However, because of the shortage of building energy management specialists and incompatibility among the energy management systems of different vendors, the BEMS solution can only be applied to limited buildings individually. To solve these problems, we propose a building cluster based remote energy monitoring and management (EMM) system and its functionalities and roles of each sub-system to simultaneously manage the energy problems of several buildings. We also introduce a novel energy demand forecasting algorithm by using past energy consumption data. Extensive performance evaluation study shows that the proposed regression based energy demand forecasting model is well fitted to the actual energy consumption model, and it also outperforms the artificial neural network (ANN) based forecasting model.

Vegetative Compatibility Groups in Fusarium graminearum Isolates from Corn and Barley in Korea

  • Moon, Jae-Ho;Lee, Yong-Hwan;Lee, Yin-Won
    • The Plant Pathology Journal
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    • v.15 no.1
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    • pp.53-56
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    • 1999
  • Fifty-three isolates of Fusarium graminearum were obtained from corn and barley samples in several provinces of Korea. Gas chromatography-mass spectrometric analysis of trichothecenes produced by these isolates revealed that 37 and 16 isolates were nivalenol (NIV)- and deoxynivalenol (DON)-chemotypes, respectively. Two hundred and seventy-five nitrate-nonutilizing (nit) mutants were obtained from the isolates. Of these mutants, 187 were identified as nit1, nit3, or NitM, but 88 could not be identified as one of these classes. The highest frequency of nit mutant was nit1 (65%), followed by nit3 (20%) and NitM (15%). Higher frequency of NitM was observed in DON-chemotypes than in NIV-chemotypes. The mutants were used for vegetative compatibility group (VCG) analysis by examining heterokaryosis using complementary mutant pairs. No heterokaryon formation was observed among all 1,248 pairwise combinations, suggesting that all isolates tested belong to different VCGs. Higher frequency of self-incompatibility was observed in NIV-chemotypes than in DON-chemotypes. These results suggest that the like-lihood of asexual genetic recombination may be very low I F. graminearum under the field condition.

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