• 한국임상수의학회지
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• 제16권1호
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• pp.118-127
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• 1999

The aim of this study was to establish the method of differential diagnosis between developing and regressing corpus luteum in cows. Plasma progesterone (P$_4$) concentrations were determined by radioimmunoassay in slaughtered, cycling and pregnant cows. Ultrasonography was used to measure the corpus luteum size and histogram values for determining the correlationships between corpus luteum area or histogram values and plasma P$_4$ concentrations. The corpora lutea were monitored in vitro (water-bath scanning) by using ultrasonography with 7.5 MHz linear-array transducer in 196 slaughtered cows. The correlation coefficient between corpus luteum area and plasma P$_4$ concentrations was 0.46 (p<0.01), and between histogram values and plasma P$_4$ concentrations was -0.44 (p<0.01), respectively. The corpora lutea were monitored by ultrasonography with 5.0 MHz linear-array transrectal transducer in 188 cycling and 30 pregnant cows. The corpus luteum areas and plasma P4 concentrations were significantly different between regressing and other corpora lutea (p<0.01), and also histogram values were significantly different between regressing and developing corpola lutea (p＜0.01). The correlation coefficients between corpus luteum areas and plasma P$_4$ concentrations were 0.76 (p<0.01), 0.71 (p<0.01), 0.65 (p＜0.05) and 0.68 (p<0.05), and between histogram values and plasma P$_4$ concentrations were 0.74 (p<0.05), 0.71 (p＜0.01), -0.52 (p<0.05) and 0.65 (p＜0.05) in developing, functional, regressing and pregnant corpora lutea, respectively. These results indicate that corpus luteum areas and plasma P$_4$ concentrations were highly correlated in all stages of corpus luteum. The histogram values and plasma P$_4$ concentrations were positive correlated in developing, functional and pregnant corpora lutea, but negative correlated in regressing corpus luteum. Therefore, the measurement of corpus luteum area and histogram value by ultrasonography is reliable method for the assessment of luteal function, specially developing and regressing corpus luteum.

• 대한임상검사과학회지
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• 제50권1호
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• pp.20-28
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• 2018

결핵(TB)은 Mycobacterium tuberculosis (MTB) 복합체의 구성원에 의한 세균 감염 질병이다. 결핵은 전 세계 인구의 1/3이 감염된 것으로 알려져 있으며, 한국에서는 매년 약 4만 명의 새로운 결핵환자가 발생한다. 또한, 비결핵 항상균 감염이 증가하고 있는 추세이다. 전통적인 결핵 및 비결핵 항상균 진단방법은 세균 배양으로 3~4주 이상이 소요된다. 따라서, 신속하고 정확한 결핵균(TB) 및 비결핵 항상균(NTM) 진단법의 필요성이 요구되고 있다. 결핵균 및 비결핵 항상균을 구분하기 위하여, 전 세계적으로 다양한 진단 방법이 개발되고 있다. 특히, 결핵균과 비결핵 항상균을 신속하고 정확한 동정의 요구가 증가함에 따라, 정확하고 신속하게 진단하기 위한 체외 진단 방법이 개발 되고 있다. 그러나 현재 결핵과 비결핵 항상균에 대한 체외 진단 시약의 성능 평가는 부족한 실정이다. 최근 식약청은 결핵균 및 비결핵 항상균 체외 진단 시약에 대한 가이드 라인을 발표했다. 본 연구에서는, 미국 FDA에 승인을 받은 결핵균 및 비결핵 항산균에 대한 체외 진단 시약의 성능을 검토하였다. 이 검토는 결핵균 및 비결핵 항상균 체외 진단 시약 평가에 유용한 참고 자료가 될것으로 사료된다.

• Journal of Ginseng Research
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• 제47권4호
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• pp.515-523
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• 2023

Background: 20(S)-protopanaxadiol (PPD), one of the main components of ginseng, has anti-inflammatory, anti-estrogenic, and anti-tumor activities. It is known that activated hepatic stellate cells (HSCs) are the primary producers of extracellular matrix (ECM) in the liver, and the Wnt/β-catenin pathway participates in the activation of HSCs. We aimed to explore whether PPD inhibits liver fibrosis is associated with the Wnt/β-catenin pathway inactivation. Methods: The anti-fibrotic roles of PPD were examined both in vitro and in vivo. We also examined the levels of Wnt inhibitory factor 1 (WIF1), DNA methyltransferase 1 (DNMT1) and WIF1 methylation. Results: PPD obviously ameliorated liver fibrosis in carbon tetrachloride (CCl₄)-treated mice and reduced collagen deposition. PPD also suppressed the activation and proliferation of primary HSCs. Notably, PPD inhibited the Wnt/β-catenin pathway, reduced TCF activity, and increased P-β-catenin and GSK-3β levels. Interestingly, WIF1 was found to mediate the inactivation of the Wnt/β-catenin pathway in PPD-treated HSCs. WIF1 silencing suppressed the inhibitory effects of PPD on HSC activation and also restored α-SMA and type I collagen levels. The downregulation of WIF1 expression was associated with the methylation of its promoter. PPD induced WIF1 demethylation and restored WIF1 expression. Further experiments confirmed that DNMT1 overexpression blocked the effects of PPD on WIF1 expression and demethylation and enhanced HSC activation. Conclusion: PPD up-regulates WIF1 levels and impairs Wnt/β-catenin pathway activation via the downregulation of DNMT1-mediated WIF1 methylation, leading to HSC inactivation. Therefore, PPD may be a promising therapeutic drug for patients with liver fibrosis.

• 세라미스트
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• 제22권3호
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• pp.269-280
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• 2019

Ceramic layered double hydroxide (LDH) nanohybrids have attracted considerable interest in biomedical science due to their unique structural feature and characteristics in biological condition. Many studies on LDH nanoparticles have been reported in diagnosis applications including magnetic resonance imaging (MRI) contrast agents in order to not only provide better imaging performance through multimodal imaging strategy, but realize therapeutic function which treat cancers in one platform. This review highlights the recent progress in MRI T₁ contrast agent, dual modal imaging system, and MRI-guided drug delivery systems ranging from synthetic method and characterization to evaluation in vitro and in vivo based on the ceramic LDH nanohybrids. Future research directions are also suggested for next-generation bio-imaging contrast agent.

• Journal of Medicine and Life Science
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• 제15권1호
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• pp.1-5
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• 2018

Allergy is conditions when a hypersensitivity reaction happens with a certain element, called as an allergen, which is commonly not reactive to ordinary individuals. Allergic diseases involve various organs or systems in the body. The purpose of allergy tests is to make a diagnosis of allergic diseases and to identify the affecting allergens. In vivo tests, more relevant in clinical situation, include skin test, patch test and provocation test. In in vitro tests, there are specific IgE test, histamine releasing assay, and lymphocyte activation test, safer and more objective than in vivo tests. In the view point of clinical practice, skin test, provocation test, total IgE test and specific IgE test were reviewed in depth.

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1993년도 추계학술대회
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• pp.63-66
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• 1993

Localized NMR spectra were obtained from phantom by OSIRIS technique. The selected volume, which can be controlled by frequency and its bandwidth, was 0.125cc out of 25cc and free of contamination from outer volume. With this technique NMR spectrum of a living tissue can be obtained without biopsy. i.e. in vivo state in which the metabolism of tissue may be quite different from in in vitro state. It is expected of this technique to be useful in the study of metabolism of living tissue as well as in diagnosis of deseases.

• 대한기계학회논문집A
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• 제27권6호
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• pp.954-959
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• 2003

Colonoscopy is an important medical procedure for the diagnosis of various diseases like cancers in the colon and rectum. But it requires a lot of time for a doctor to acquire dexterous skills necessary to perform successful colonoscopy. Moreover, to many patients, conventional colonoscopy simply takes too long time. Therefore, some studies on the development of autonomous and more convenient colonoscope are carried out. In this Paper, we Propose a functional colonoscope robot system that has a locomotive function with a hollow body, a steering system, and other basic functions of typical conventional colonoscope systems. The concept and each component of the functional colonoscope system are described in this paper. In order to evaluate the functional performance of the colonoscope robot, we carried out in -vitro and in-vivo tests.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2002년도 하계종합학술대회 논문집(5)
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• pp.277-280
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• 2002

In this paper, it is fundamental purpose that finding the relationship between quality and structure of bone, by reconstructing the bone structure image, using ultrasound. In this study, longitudinal transmission method was used for experiment as basic measuring method, which is known as ultrasonic diagnosis method for human tibia. And using ultrasonic velocity parameter that can be detected and calculated with the transmitted signal, new estimated parameter, called Bone Area Fraction, is applied to reconstruct image. Through the in-vitro experiment in cattle's tibia bone, basic sectional image of bone which is similar in real bone structure image can be reconstructed.

• Journal of Oral Medicine and Pain
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• 제12권1호
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• pp.17-26
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• 1987

In order to investigate the biostimulatory effect of low power density laser radiation in vitro, human gingival fibroblasts were cultured in MEM in which experiment groups respectively were made to 30 sec, 60 sec and 90 sec group. The experiments were performed by cell count, DNA and protein content measurements after experimental groups were irradiated with GaAlAs laser every day by forth day and then control group and experimental groups were compared. The results were as follows: 1. Cell counts of experimental groups were increased with exposure time, but showed no significance (P>0.05). 2. When the protein contents were compared, there was a very significant increase in 90 sec. experimental group (P<0.01). 3. When the DNA contents were compared, there was a significant difference only between control and 70 sec. group (P<0.05).

• 한국정보통신학회논문지
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• 제24권10호
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• pp.1394-1397
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• 2020

The urine test used as a basic test method of in vitro diagnosis for health care has been used for a long time to be simple and convenient. The urine test method is using a color that appears depending on the change in the ion concentration that reacts over time buried in the standard color test paper(Strips) with a urine sample applied to some reaction reagents. In this paper, it was proposed a neural network algorithm to obtain a suitable and reproducibility and accuracy classifier suitable for the urine analysis system. The experimental results were compared with the visual colorimetric analysis, and the neural network algorithm showed better results.