• Analytical Science and Technology
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• v.28 no.2
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• pp.79-85
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• 2015

A preliminary experiment was performed to develop a fast, convenient, and economical semi-quantitative method of analyzing amphetamine-like amines from images of derivatives. These were generated from the reaction (in situ, co-spot) of three amphetamine-like compounds with three derivatization reagents on a TLC plate. The attempt was made to optimize the reaction conditions for an efficient derivatization reaction, and TLC images taken by a digital camera were analyzed using two types of image analysis program (CP Atlas 2.0 and ImageJ) for repeatability (RSD, %) and linearity (R²). Then, their results were compared. For efficient derivatization, the reaction conditions needed to be modified. The results of image analysis of each of the samples at two different concentrations (0.5 mg/mL and 0.01 mg/mL) showed that the RSD values for reaction repeatability were in the range of 0.69-5.50%. From the calibration curves between the area of the derivative and the concentration of amines, the R² values (R² > 0.9906) for good linear correlation were found to be high, in a concentration range of 0.1-0.005 mg/mL of amines. In addition, the two programs demonstrated little difference in the analysis of repeatability and linearity of the derivatization, so that the current method has the potential to be used for the semi-quantitative analysis of amines.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.3
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• pp.335-340
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• 1997

Fluorescence in situ hybridization (FISH) techniques allow the enumeration of chromosome abnormalities and from a great potential for many clinical applications. In order to produce quantitative and reproducible results, expensive tools such as a cooled CCD camera and a computer software are required. We have developed a Chromosome Image Processing System (Chips) using FISH that allows the detection and mapping of the genetic aberrations. The aim of our study, therefore, is to evaluate the capabilities of our original system using a black-and-white video camera. As a model system, three repetitive DNA probes (D18Z1, DXZ1, and DYZ3) were hybridized to variety different clinical samples such as human metaphase spreads and interphase nuclei obtained from uncultured peripheral blood lymphocytes, uncultured amniocytes, and germ cells. The visualization of the FISH signals was performed using our system for image acquisition and pseudocoloring. FISH images were obtained by combining images from each of probes and DAPI counterstain captured separately. Using our original system, the aberrations of single or multiple chromosomes in a single hybridization experiment using chromosomes and interphase nuclei from a variety of cell types, including lymphocytes, amniocytes, sperm, and biopsied blastomeres, were enabled to evaluate. There were no differences in the image quality in accordance with FISH method, fluorochrome types, or different clinical samples. Always bright signals were detected using our system. Our system also yielded constant results. Our Chips would permit a level of performance of FISH analysis on metaphase chromosomes and interphase nuclei with unparalleled capabilities. Thus, it would be useful for clinical purposes.

• KSBB Journal
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• v.17 no.1
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• pp.80-87
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• 2002

Laboratory scale aerobicfanaerobic biofilm reactor was used for simultaneous and stable removal of organics, N and P components to investigate optimum design and operation parameters and to analyze the microbial distribution and consortium structure of nitrification and denitrification bacteria in aerobic and anaerobic biofilm systems. The biofilm reactor was successfully operated for 143 days to show $COD_{cr},\;BOD_5$, SS removal efficiencies of 88, 88, and 97%, respectively. During the experiment period, almost complete nitrification efficiency of 96% was achieved. Denitrification efficiency was about 45% without addition of any external carbon sources. In case of total phosphorus removal, 74% of the inlet phosphorus was removed. Fluorescence in situ hybridization (FISH) results showed that most of the ammonia oxidizing bacteria in the aerobic nitrification zone was found to be Nitrosomonas species while Nitrospira was the representative nitrite oxidizing bacteria. For the denitrification, Rhodobacter, Rhodovulum, Roseebacter and Paracouus were the dominant denitrification bacteria which was 10 to 20% of the total bacteria in numbers.

• Economic and Environmental Geology
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• v.46 no.5
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• pp.425-444
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• 2013

This study was carried out in order to determine the remediation of total petroleum hydrocarbons (TPHs), trichloroethylene (TCE), perchloroethylene (PCE), benzene, toluene, ethylbenzene and xylenes (BTEX) compounds for non-aqueous phase liquids (NAPLs) using in-situ air sparging (IAS) / vapor extraction (VE) with the marine sediments of Mandol, Hajeon, Sangam and Busan, South Korea. Surface sediment of Mandol area had sand characteristics (average particle size, 1.789 ${\Phi}$), and sandy silt characteristics (average particle size, 5.503 ${\Phi}$), respectively. Sangam surface sediment had silt characteristics (average particle size, 5.835 ${\Phi}$). Sediment characteristics before experiment in the Busan area showed clay characteristics (average particle size, 8.528 ${\Phi}$). TPHs level in the B1 column of Mandol, Hajeon, Sangam, and Busan sediments were 2,459, 6,712, 4,348, and 14,279 ppm. B2 (3 L/min) to B5 (5 L/min) columns reduced 99.5% to 100.0% of TCE and 93.2% to 100.0% of PCE. Removal rates of TCE, PCE, and BTEX are closely correlated (0.90-0.99) with particle sizes and organic carbon concentrations. However, TPHs (0.76) and benzene (0.71) showed the poorer but moderate correlations with the same parameters.

• Journal of the Korean Association of Geographic Information Studies
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• v.21 no.3
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• pp.162-175
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• 2018

The purpose of this study was to analyze the surface temperature of surface fabrics using UAV TIR images, to mitigate problems in the thermal environment of urban areas. Surface temperature values derived from UAV images were compared with those measured in-situ during the similar period as when the images were taken. The difference in the in-situ measured and UAV image derived surface temperatures is the highest for gray colored concrete roof fabrics, at $17^{\circ}C$, and urethane fabrics show the lowest difference, at $0.3^{\circ}C$. The experiment power of the scatter plot of in-situ measured and UAV image derived surface temperatures was 63.75%, indicating that the correlation between the two is high. The surface fabrics with high temperature are metal roofs($48.9^{\circ}C$), urethane($43.4^{\circ}C$), and gray colored concrete roofs($42.9^{\circ}C$), and those with low temperature are barren land($30.2^{\circ}C$), area with trees and lawns($30.2^{\circ}C$), and white colored concrete roofs($34.9^{\circ}C$). These results show that accurate analysis of the thermal characteristics of surface fabrics is possible using UAV images. In future, it will be necessary to increase the usability of UAV images via comparison with in-situ data and linkage to satellite imagery.

• Advances in Computational Design
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• v.4 no.2
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• pp.141-153
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• 2019

This research is comprised of virtually simulating behavior while experiencing low gravity effects in advance of real world testing in low gravity aboard Zero Gravity Corporation's (Zero-G) research aircraft (727-200F). The experiment simulated a drill rig penetrating a regolith simulant. Regolith is a layer of loose, heterogeneous superficial deposits covering solid rock on surfaces of the Earth' moon, asteroids and Mars. The behavior and propagation of space debris when drilled in low gravity was tested through simulations and visualization in a leading dynamic simulation software as well as discrete element modeling software and in preparation for comparing to real world results from flying the experiment aboard Zero-G. The study of outer space regolith could lead to deeper scientific knowledge of extra-terrestrial surfaces, which could lead us to breakthroughs with respect to space mining or in-situ resource utilization (ISRU). These studies aimed to test and evaluate the drilling process in low to zero gravity environments and to determine static stress analysis on the drill when tested in low gravity environments. These tests and simulations were conducted by a team from Texas A&M University's Department of Construction Science, the United States Air Force Academy's Department of Astronautical Engineering, and Crow Industries

• Journal of Environmental Science International
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• v.17 no.1
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• pp.97-105
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• 2008

This study examined the contaminated soils with an indicator of TPH using SVE (Soil Vapor Extraction) and biological treatments. Their results are as follows. Water content in the polluted soils slowly decreased from 15% during the initial experimental condition to 10% during the final condition. Purification of polluted soils by Bioventing system is likely to hinder the microbial activity due to decrease of water content. Removal rate of TPH in the upper reaction chamber was a half of initial removal rate at the 25th day of the experiment. The removal rate in the lower reaction chamber was 45% with concentration of 995.4 mg/kg. When the Bioventing is used the removal rate at the 14th day of the experiment was 53%, showing 7 day shortenting. Since the Bioventing method control the microbial activity due to dewatering of the polluted soil, SVE method is likely to be preferable to remove in-situ TPH. The reactor that included microbes and nutrients showed somewhat higher removal rate of TPH than the reactor that included nurtients only during experimental period. In general, the concentration showed two times peaks and then decreased, followed by slight variation of the concentration in low concentration levels. Hence, in contrast to SVE treatment, the biological treatment tend to show continuous repetitive peaks of concentration followed by concentration decrease.

• Magazine of the Korean Society of Agricultural Engineers
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• v.40 no.2
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• pp.112-122
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• 1998

Measurement of electrical resistivity in soils has been used for many years with purpose of estimating in situ porosity or density. Recently electrical resistivity has also been used as an indicator of soil contaimination. This paper presents the result of laboratory experiment to investigate the resistivity variation in contaminated sandy soils. The results can be used with the Cone Penetrometer Test (CPT) result to analyse ground condition. In the experiment, the water content and leachate concentration of soils were controlled by groundwater and leachate, and then the resistivity measurement was made with 'STING-R1' by Advanced Geosciences Inc. In the case of using groundwater, the resistivity was in the range of over 1000${\omega}{\cdot}m$, but in the case of using polluted water by leachate, the resistivity decreased significantly down to 10~ 100${\omega}{\cdot}m$ for the same moisture content. Also the resistivity varied according to the degree of saturatrion. Therefore, if soil is contaminated by leachate, the CPT with electrical resistivity sensor might be used to investigate the contamination status and plume migration. But exact component of leachate and the pollutant concentration are still hard to identify.

• Korean Journal of Agricultural Science
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• v.40 no.1
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• pp.13-18
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• 2013

Effect of electric pulse stimulation was tested on the fruit body formation of valuable mushroom Tricholoma matsutake in the field of natural habitat of this mushroom. After applying the electric stimulator to the specific area of pine forest we found that the treatment especially stimulated the fructification of T. matsutake. And the most valuable findings of our study was that only our treatment plots showed fruit body formation whereas the control plots and the whole natural habitats of our study area showed zero production of this mushroom during the same time. From the point of view of mushroom production, our experiment shows that fruit body production can be upgraded by using pulsed power as an electrical stimulation in the field of the natural habitat of this mushroom. These findings from our experiment confirm the effectiveness of the significance of pulsed power technology for the improvement of T. matsutake fruit body production in the natural habit of this mushroom.

• Ocean and Polar Research
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• v.43 no.3
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• pp.193-203
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• 2021

In-situ experiment using ocean buoys is a direct ocean observation and has been playing an important role from the past to the present based on high reliability. The ocean buoy is operated more stable than before due to the technological development of communication (GPS, satellite, …) and equipment, but still moored buoys are not free from various accidents occurring in the ocean. Nevertheless, there is currently a lack of countermeasures or manuals about mooring accidents. Therefore, in this study, based on the experience of operating buoys conducted for many years, the advantages and disadvantages of ocean buoys according to size were analyzed. and legal procedures before and after buoy mooring were presented to enhance the use of buoys. And it is suggested to realize successful experiment by proposing considerations before mooring the buoy in preparation for an accident.