• Bulletin of the Korean Chemical Society
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• v.34 no.9
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• pp.2635-2639
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• 2013

The rapid and accurate identification of biological agents is a critical step in the case of bio-terror and biological warfare attacks. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry has been widely used for the identification of microorganisms. In this study, we describe a method for the rapid and accurate discrimination of Bacillus anthracis spores using MALDI-TOF MS. Our direct in-situ analysis of MALDI-TOF MS does not involve subsequent high-resolution mass analyses and sample preparation steps. This method allowed the detection of species-specific biomarkers from each Bacillus spores. Especially, B. anthracis spores had specific biomarker peaks at 2503, 3089, 3376, 6684, 6698, 6753, and 6840 m/z. Cluster and PCA analyses of the mass spectra of Bacillus spores revealed distinctively separated clusters and within-groups similarity. Therefore, we believe that this method is effective in the real-time identification of biological warfare agents such as B. anthracis as well as other microorganisms in the field.

• Nuclear Engineering and Technology
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• v.52 no.6
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• pp.1259-1265
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• 2020

A beta ray scanner was proposed for in-situ discrimination of beta and gamma ray radioactivity. This scanner is based on the principle that gamma and beta rays experience different changes in detection efficiency in scintillators with different geometries, especially with regard to the scintillator thickness. The ratios of the counting rates of gamma rays (R_gamma), beta rays (R_beta), and sample measurements (R_total) in a thick scintillator to those in a thin one are reported. The parameter X_thick, which represents the counting rate contributed by beta rays to the total counting rate in the thick scintillator, was derived as a function of those ratios. The values of R_gamma and R_beta for ⁶⁰Co and ⁹⁰Sr sources were estimated as 3.2 ± 0.057 and 0.99 ± 0.0049, respectively. The estimated beta ray contributions had relative standard deviations of 2.05-4.96%. The estimated range of the beta rays emitted from ⁹⁰Sr was 19 mm as per the Monte Carlo N-Particle simulation, and this value was experimentally verified. Homogeneous and surface contaminations of ⁶⁰Co and ⁹⁰Sr-⁹⁰Y were simulated for application of the proposed method. The counting rate contributed by the beta rays was derived and found to be proportional to the concentration of ⁹⁰Sr-⁹⁰Y contamination.

• Proceedings of the KSRS Conference
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• 2004.10a
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• pp.140-143
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• 2004

The west coast of the Korean Peninsula is famous for its large tidal range (up to 9 m) and vast tidal flats. With comparison the sedimentary environments of open and close tidal flat using remote sensing, we select Kanghwa tidal flat and Hwang-Do tidal flat in Cheonsu Bay. Prior to surface sediment discrimination using remote sensing, sedimentary environments including intertidal OEM, hydraulic condition, and relationship between grain size and various tidal condition are investigated. Remote sensing has the potential to provide synoptic information of intertidal environments. The objectives of this study are: (i) to generate an intertidal digital elevation model (OEM) using the waterline method of Lansat TM/ETM+, (ii) to investigate the tidal channel distribution using texture analysis, and (iii) to analyze the relationship between surface grain size by using in-situ data and intertidal OEM and tidal channel density by using high-resolution satellite data such as IKONOS and Kompsat EOC. The results demonstrate that satellite remote sensing is an efficient and effective tool for a surface sediment discrimination and long term morphologic change estimation in tidal flats.

• Korean journal of applied entomology
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• v.42 no.4
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• pp.361-365
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• 2003

Several species of planthoppers such as brown planthopper, N. lugens (Stal) (BPH), smaller brown planthopper, Laodelphax striatellus (Fall n) (SBPH), and white-backed planthopper, Sogatella furcifera (Horv th) (WBPH) are distributed in Korean rice paddies but not easy to discriminate them in situ. Accurate discrimination of them is an indispensable process in the forecasting for their outbreak and control. Especially, innovative discrimination method for BPH was required because BPH was one of the most important insect pest of rice. Nymphs and adults of BPHs, SBPHS, and WBPHS, thus, were examined their morphological characteristics in the paddies and laboratory. The nymphs of BPH had different characters from those of SBPH and WBPH. The nymphs of BPH had white fluorescent spot between bottom of compound eye and antenna, while there was no that spot the other two species. The white spot was the brightest at the nymphs just after hatching and getting weaker as the nymph developed. At last the white spot was totally disappeared at the adult stage. This white spot was innovative criterium to discriminate nymphs of BPH, SBPH, and WBPH in rice paddies.

• Journal of Radiation Protection and Research
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• v.41 no.2
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• pp.87-91
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• 2016

Background: A hand-held detector for an emergency response was developed for nuclide identification and to estimate the information of the ambient dose rate in the scene of an accident as well as the radioactivity of the contaminants. Materials and Methods: To achieve this, the most suitable sensor was first selected as a cadmium zinc telluride (CZT) semiconductor and the signal processing unit from a sensor and the signal discrimination and storage unit were successfully manufactured on a printed circuit board. Results and Discussion: The performance of the developed signal processing unit was then evaluated to have an energy resolution of about 14 keV at 662 keV. The system control unit was also designed to operate the CZT detector, monitor the detector, battery, and interface status, and check and transmit the measured results of the ambient dose rate and radioactivity. In addition, a collimator, which can control the inner radius, and the airborne dust sampler, which consists of an air filter and charcoal filter, were developed and mounted to the developed CZT detector for the quick and efficient response of a nuclear accident. Conclusion: The hand-held CZT detector was developed to make the in-situ gamma-ray spectrometry and its performance was checked to have a good energy resolution. In addition, the collimator and the airborne dust sampler were developed and mounted to the developed CZT detector for a quick and efficient response to a nuclear accident.

• Proceedings of the KSRS Conference
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• 1999.11a
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• pp.396-402
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• 1999

The aim of this study is to assess the OSMI (Ocean Scanning Multi-spectral Imager), whose central bands are 443nm, 490nm, 510nm, 555nm, 670nm, and 865nm, for agricultural applications. Radiance measurements, used to determine per cent reflectance of canopies and soils, were acquired with spectro-radiometers (Li-1800;330~1,100nm, GER-SFOV;350 ~2,500nm, and MSR-7000; 300~2,500nm) in situ for crops and indoors for soils. OSMI equivalent bands and their ratio values were prepared(20nm interval for bands 1~5; 4nm interval for band 6) by averaging spectral reflectance values to the real OSMI bands and analyzed as to crop growth parameters, leaf area index (LAI), total dry matter, and growth index in crops and physiochemical properties in soils. Spectral variations for each growth stage in rice and for crop discrimination in upland crops were significant statistically. In soils, clay and water content, CEC (Cation Exchange Capacity), free iron oxide, and some cation content were correlated with the OSMI equivalent bands. The result of this study shows OSMI wave bands would be promising for agricultural application in terms of spectral information and resolution.

• Korean Journal of Remote Sensing
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• v.15 no.4
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• pp.307-319
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• 1999

The aim of this study is to assess the OSMI (Ocean Scanning Multi-spectral Imager), whose central bands are 443nm, 490nm, 510nm, 555nm, 670nm, and 865nm, for agricultural applications. Radiance measurements, used to determine per cent reflectance of canopies and soils, were acquired with spectro-radiometers (Li-1800;330∼1,100nm, GER-SFOV;350∼2,500nm, and MSR-7000; 300∼2,500nm) in situ for crops and indoors for soils. OSMI equivalent bands and their ratio values were prepared(20nm interval for bands 1∼5; 40nm interval for band 6) by averaging spectral reflectance values to the real OSMI bands and analyzed as to crop growth parameters, leaf area index (LAI), total dry matter, and growth index in crops and physiochemical properties in soils. Spectral variations for each growth stage in rice and for crop discrimination in upland crops were significant statistically. In soils, clay and water content, CEC (Cation Exchange Capacity), free iron oxide, and some cation content were correlated with the OSMI equivalent bands. The result of this study shows OSMI wave bands would be promising for agricultural application in terms of spectral information and resolution.

• Applied Biological Chemistry
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• v.39 no.4
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• pp.309-314
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• 1996

2-Furyltriisopropoxytitanium was synthesized in situ through transmetallation of 2-furyllithium with chlorotitaniumtriisopropoxide. The compound could be isolated at room temperature and preserved at $-10^{\circ}C$ for several weeks. The reactivity 2-furyltriisopropoxytitanium to carbonyl compounds proved to be high. A complete aldehyde selectivity was observed in competition reactions of 2-furyltriisopropoxytitanium with a 1:1 mixture of aldehyde and ketone. In competition reactions of 2-furyltriisopropoxytitanium with a 1 : 1 mixture of ketone and ketone, the degree of ketone / ketone discrimination was substantial. In the reaction of 2-furyltriisopropoxytitanium to ketone-ester function, the reagent was solely reacted with the ketone function.

• Journal of the Korean Society for Nondestructive Testing
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• v.28 no.4
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• pp.315-322
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• 2008

Valves in power plants are leaking internally by various damages including insertion of foreign objects on seat, seat crack, defects and fatigue crack of stem packing or welds etc. due to severe operating conditions such as high temperature and high pressure for extended period time. Acoustic emission(AE) technology should be applied in order to diagnose precisely and evaluate these valve internal leak. In this paper, results of studies which have accomplished in actual power plant are presented. We have analyzed background noise, AE signal level and frequency spectrum through laboratory tests on the basis of various actual conditions in power plant, and also have considered evaluation methods on the background noise, AE properties and the detectable minimum leak rate according to valve leak conditions through comparing with results of field tests in power plant. As a result of these studies, we conformed that evaluation of internal leak conditions including discrimination of leak or not, and the detectable minimum leak rate is possible, and also it is expected to contribute to safe operation and prevention of energy loss in power plants.

• Journal of Life Science
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• v.13 no.2
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• pp.163-167
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• 2003

Identification of species within the toxin-producing genus Alexandrium is vital for biotoxin monitoring and mitigation decisions regarding shellfish industry. In particular, the discrimination of resting cysts of only A. tamarense from that of Alexandrium spp. is considerable important to fundamentally monitor and predict this species before vegetative cells occur in the nature. Fluorescent cTAM-F1 DNA probe was responsible to not only binding the activity of the vegetative cells in A. tamarense, but also to the resting cysts, which was treated with methanol after fixation and stained by primuline on the surface The location of fluorescence in cultured vegetative cells and resting cysts was almost at tile bottom of the nucleus. The optimal incubation temperature and time using in situ hybridization were 50-$54^{\circ}C$ and 40-60 min, respectively, to penetrate the DNA probe into cell.