• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.6
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• pp.1393-1403
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• 2003

The genetic effects of restraint stress challenge on HPA axis and the therapeutic effect of Boshimgeonbi-Tang on the stress were studied with cDNA microarray analyses on hypothalamus using an immobilization-stress mouse as stress model. Male CD-1 mice were restrained in a tightly fitted and ventilated vinyl holder for 2hours once a day, and this challenge was repeated for seven consecutive days. The body weights of the immobilization-stress mice were diminished about 25 percent degree as compared to normal ones. Seven days later, total RNA was extracted from the organs of the mouse, body-labeled with CyDye/sup TM/ fluorescence dyes (Amersham Bioscience Co., NJ), and then hybridized to cDNA microarray chip. Scanning and analyzing the array slides were carried out using GenePix 4000 series scanner and GenePix Pro/sup TM/ analyzing program, respectively. The expression profiles of 109 genes out of 6000 genes on the chip were significantly modulated in hypothalamus by the immobilization stress. Energy metabolism-, lipid metabolism-, apoptosis- and signal transduction-related genes were transcriptionally activated whereas DNA repair-, protein biosynthesis-, and structure integrity-related genes were down-regulated in hypothalamus. The 58 genes were up-regulated by the mRNA expression folds of 1.5 to 7.9. and the 51 genes were down-regulated by 1.5 - 3.5 fold. The 20 genes among them were selected to confirm the expression profiles by RT-PCR. The mRNA expression levels of Tnfrsf1a (apoptosis), Calm2 (cell cycle), Bag3 (apoptosis), Hspe1 (protein folding), Aatk (apoptosis), Dffa (apoptosis), Itgb1 (cell adhesion), Vcam1 (cell adhesion), Fkbp5 (protein folding), BDNF (neuron survival) were restored to the normal one by the treatment of Boshimgeonbi-Tang.

• Proceedings of the KAIS Fall Conference
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• 2008.11a
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• pp.430-432
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• 2008

학교와 같이 대단위 교육을 수행하는 곳에서 필수적으로 사용되는 것은 강의실 의자이다. 소비자 중심적인 시대적 변화에 따라 많은 제품들이 다양한 소비자의 요구가 반영되어 출시되고 있으나, 강의실 의자는 아직 이러한 요구가 받아들여지지 않거나 무시되고 있다. 강의실에서 학생들이 가장 불편해 하는것은 자신의 신체치수에 적합하지 않는 강의실 의자이다. 따라서 본 연구에서는 다양한 신체치수를 반영할 수 있는 가변형 강의실용 의자를 개발하고자 한다. 신체치수는 앉은키와 체형을 고려하여 앉고 일어서는데 불편함이 없도록 책상 접힘과 의자와의 거리조절이 가능하고, 사람의 신장에 따라 높낮이 조절이 가능한 구조로 설계하여 기존의 평균책상 사이즈에 구애받지 않고 사용자의 신체구조에 따라 자유롭게 조절시켜 장시간 의자에 앉아 있을 때 불편함을 개선하고 불균형한 자세 등을 교정시켜 줌으로써, 학업 시 바른 자세를 유도하며 학업능률을 향상시킬 것으로 기대한다.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.345-349
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• 2007

We wished to create a set of small molecular weight PDZ domain ligands that may be used in functional studies on the proteins AF6, PSD-95 and Shank. As a starting point, the Shank3 PDZ domain was cloned, purified, and characterized the structure of Shank3 PDZ domain by 1D $^1H-NMR$. The chemical shift dispersion of the proton signals indicates that the purified Shank3 PDZ protein is very pure and globally well folded. Currently, we are working on improving the yield of the protein production for complete NMR structural analysis of the Shank3 PDZ domain.

• BMB Reports
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• v.57 no.8
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• pp.369-374
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• 2024

Antigen 43 (Ag43) proteins, found on the outer membrane of Escherichia coli, are β-sheets that fold into a unique cylindrical structure known as a β-barrel. There are several known structural similarities between bacterial Ag43 autotransporters and physical components; however, the factors that stabilize the barrel and the mechanism for Ag43 passenger domain-mediated translocation across the pore of the β-barrel remain unclear. In this study, we analyzed Ag43β-enhanced green fluorescent protein chimeric variants to provide new insights into the autotransporter Ag43β-barrel assembly, focusing on the impact of the α-helical linker domain. Among the chimeric variants, Ag43β700 showed the highest surface display, which was confirmed through extracellular protease digestion, flow cytometry, and an evaluation of outer membrane vesicles (OMVs). The Ag43β700 module offered reliable information on stable barrel folding and chimera expression at the exterior of the OMVs.

• Journal of the Society of Disaster Information
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• v.8 no.3
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• pp.297-303
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• 2012

This study examines the isolation-heat performance of fire proof fabric that is equipped over ceiling board in order to secure the capability of arresting the spread of a fire at roof 'Jucsim' structure of Wooden Cultural Properties. For this examination, I conduct experiments with the model and compare two construction methods of fire proof fabric, or flat-type, folding-type. The experiments show the following results. In case of installing the fire proof fabric in flat-type, when the temperature under the ceiling board is $750^{\circ}C$, the temperatures over the ceiling board is $50^{\circ}C$; the difference is $700^{\circ}C$. Compared with the case of removing fire proof fabric, the temperature over the ceiling board is lower by approximately $580^{\circ}C{\sim}600^{\circ}C$. Therefore it can be said that the construction of fire proof fabric enhances the isolation-heat performance. In case of installing the fire proof fabric in folding-type, when the temperature under the ceiling board is $600^{\circ}C$, the temperatures over the ceiling board is $65^{\circ}C$; the difference is approximately $530^{\circ}C$. Thus its isolation-heat performance is a little inferior to the flat-type equipment.

• Clinical and Experimental Reproductive Medicine
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• v.30 no.2
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• pp.127-133
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• 2003

Objective: The aim of this study was to investigate the morphological aspects of testicular tissue before and after freezing-thawing by light and transmission electron microscopy. Methods: Tissue biopsies were carried out on mouse testis for freezing. Samples in medium containing 20% glycerol were frozen by computer-controlled freezing program. The effect of freezing-thawing on the structural change of testicular tissues were examined by light and electron microscopy. Results: The freezing-thawing procedure had no significant effect on tubular diameter. However, it caused folding of the lamina propria, and notable damage to Sertoli cells, spermatogonia and spermatocytes. The cells were detached, desquamated from the basal lamina and had increased vacuolization. Round spermatids, elongated spermatids and spermatozoa were less affected, and most of them maintained their normal structure. Conclusions: The structure of spermatogonia, spermatocyte and basal compartments in seminiferous epithelium was significantly altered by freezing-thawing procedure of mouse testicular tissues. Thus, we need to develop a more reliable method for the cryopreservation of testicular tissues.

• Journal of Korea Foundry Society
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• v.44 no.4
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• pp.97-102
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• 2024

In this study, the properties of Zr-14Cu-7.5Ni-2.6Al wide ribbon with amorphous structure and properties were analyzed using Hall effect, SEM-EDX, and XRD. Made by melt spinning method, this Zr-14Cu-7.5Ni-2.6Al based alloy ribbon is not more than 96 ㎛ thick and 100 mm wide. This amorphous alloy exhibited tensile strength of 1,641 MPa, yield strength of 1,541 MPa, elongation of 1% and elastic modulus of 98GPa. The bulk concentration, resistivity, and mobility values are midway between general heavy doping ceramics and metals, and they are about 100 times weaker than ordinary metals, so they are close to Si and have good electrical conductivity. In addition, folding tests were conducted at extreme temperatures, withstanding 150,000 times at -20℃, 300,000 times at 24℃, and 150,000 times at 60℃, with no folding defects observed. These results demonstrate the excellent durability and reliability of the Zr-14Cu-7.5Ni-2.6Al wide ribbon alloy and suggest the possibility of developing electronic products using this alloy.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2001.06a
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• pp.149-153
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• 2001

To investigate the mode of bactericidal action for antimicrobial peptide, pediocin, synthetic and mutant pediocins were prepared by direct chemical synthesis. Native pediocin was purified from Pedio-coccus acidilactici M and its conformational structure and bactericidal functions were analyzed and compared to synthetic pediocin. Schematic mode of pediocin actions, how pediocin binds on the target cell membrane, penetrates and makes tunnel are proposed. For these purposes, primary and secondary structures of pediocin was analyzed and disulfide bond assignment was also done. The pediocin purified from P. acidilactici M had high effective bactericidal ability against gram positive bacteria, especially Listeria monocytogenes and was very stable at extreme pHs and even at high temperatures such as autoclaving temperature (121$^{\circ}C$). Pediocin was consisted of 44 amino acids with four cysteines. Novel synthetic peptides were achieved by solid phase peptide synthesis(SPPS) method. To explain the function of cysteine in C-terminal region, mutant pediocin, Ped[C24A＋C44A], was synthesized and their structural and biological functions were analyzed. Second mutant pediocin, Ped[KllE], was prepared to explain the function of lysine at 11 of N-terminal part of pediocin, especially loop of $\beta$-sheet, and to predict the initial binding site of pediocin. The native and synthetic pediocins was showed random coil conformation by spectropolarimetry in moderate conditions. This conformation was observed in extreme conditions such as high temperature and low and high pHs, also. Circular dichroism(CD) data also showed the existence of $\beta$-turn structure in N-terminal part both native and synthetic pediocins. A structural model for pediocin predicts that 18 amino acids in the N-terminal part of the peptide assume a three-strand $\beta$-sheet conformation. This random coil in C-terminal part of pediocin was converted to folding structure, helix structure, in nonpolar solvents such as alcohol and TFE. The disulfide bond between $^{9}$ Cys and $^{14}$ Cys was concrete and inevitable, however, evidences of disulfide bond between $^{24}$ Cys and $^{44}$ Cys was not. Data of Ped[C24A＋C44A], pediocin mutant showed that $^{44}$ Cys was required during killing the target cells but not inevitable, since Ped[C24A＋C44A] still have bactericidal activity but much less than native pediocin. Another pediocin mutant, Ped[KllE], had still bactericidal activity, was controversial to propose that positive charge like as $^{11}$ Lys in loop or hinge in bacteriocin bound or helped to binding to microorganism with electrostatic interaction between cell membrane especially teichoic acid and positive amino acid nonspecifically. The conformation of pediocin among native, synthetic and mutant pediocins did not show big difference. The conformations between oxidized and reduced pediocin were almost similar regardless of native or synthetic.

• Journal of the Korean earth science society
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• v.42 no.5
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• pp.479-495
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• 2021

This study investigates the synoptic (patterns of southern highs, northern lows, and lows rapidly developed by tropopause folding), thermodynamic, and kinematic characteristics of a strong wind that occurred in the Yeongdong region of South Korea on March 18-20, 2020. To do so, we analyzed data from an automatic weather station (AWS), weather charts, the European Centre for Medium-range Weather Forecasts (ECMWF) reanalysis, rawinsonde, and windprofiler radars. The daily maximum instantaneous wind speed, exceeding 20 m s^-1, was observed at five weather stations during the analysis period. The strongest instantaneous wind speed (27.7 m s^-1) appeared in the Daegwallyeong area. According to the analysis of weather charts, along with the arrangement of the north-south low-pressure line, the isobars were moved to the Yeongdong area. It showed a sine wave shape, and a strong wind developed owing to the strong pressure gradient. On March 19, in the northern part of the Korean Peninsula, with a drop in atmospheric pressure of 19 hPa or more within one day, a continuous strong wind was developed by the synoptic structure of the developing polar low. In the adiabatic chart observed in Bukgangneung, the altitude of the inversion layer was located at an altitude of approximately 1-3 km above the mountaintop, along with the maximum wind speed. We confirmed that this is consistent with the results of the vertical wind field analysis of the rawinsonde and windprofiler data. In particular, based on the thermodynamic and kinematic vertical analyses, we suggest that strong winds due to the vertical gradient of potential temperature in the lower layer and the development of potential vorticity due to tropopause folding play a significant role in the occurrence of strong winds in the Yeongdong region.

• Economic and Environmental Geology
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• v.6 no.2
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• pp.81-114
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• 1973

The purpose of the present study is to clarify the stratigraphy and geologic structure of the Great Limestone Series by means of study on fossil conodonts and detail investigation of geologic structure. In recent years very few geologists in Korea argue without confident evidences against the age and stratigraphy of the Great Limestone Series which have been rather well established previously in most parts of the regions although it is ambiguous and has not been studied in other areas. Five type localities in the Kangweon basin where the Great Limestone Series is well cropped out were chosen for the study. Total 26 genus and 66 species of conodont were identified from 290 samples collected and treated. From the study on conodonts the age of each formations of the Great Limestone Series has been determined as follows: The Great Limestone Series of Duwibong type Duwibong limestone: Caradocian (mid-Ord.) Jikunsan shale: Landeilian (mid-Ord.) Maggol limestone: Llanvirn-Llandeilian (mid-Ord.) Dumugol: Arenigian (Ord.) Hwajeol: Upper Cambrian The Great Limestone Series of Yeongweol type Mungok (Samtaesan) : Ordovician Machari: upper Cambrian The Great Limestone Series of Jeongseon type Erstwhile Jeongseon limestone: mid-Ord. The erstwhile Jongseon Limestone formation in Jeongseon district is separated into Hwajeol, Dongjeom, Dumudong, and Maggol formations which were cropped out repeatedly by folding and faulting, but Maggol is predominant in areal distribution. Yemi Limestone Breccia bed is not a single bed but distributed in several horizons so that it bears no stratigraphic significance. The limestone bed above Yemi Limestone Breccia, which was believed by some geologists to be much younger than Ordovician, is identified to be Maggol and its age is determined to be mid-Ordovician. Sambangsan formation in Yeongweol district was believed to be Cambrian age and lower horizon than Machari formation by Kobayashi, but C. M. Son believed that it might belong to later than Ordovician and lies above the Great Limestone Series of Yeongweol type. It was identified to be upper Cambrian and lies beneath the Machari formation and above the Daeki formation, the lower most horizon of the Great Limestone Series. The age of Yeongweol type Choseon system is contemporaneous with that of Duwibong type Choseon system. The difference in lithofacies is not due to lateral facies change, but due to the difference in its depositional environment. The Yeongweol type Choseon system is believed to be deposited in the small Yeongweol basin which was separated from the main Kangweon sedimentary basin. Judging from these facts it is definitely concluded that there exists no Gotlandian formation in the regions studied. Structurally the Kangweon basin comprises five basins and two uplifted areas. These structures were originated by at least two crustal movements, that is, Songrim disturbance of Triassic and Daebo orogeny of Jurasic age.