• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.26-26
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• 2003

Hyaluronic acid (HA)-binding sites have been shown the diagnostic potential fur assessment of sperm maturity, which is related to male fertility. This study was designed to evaluate chromosomal patterns in porcine embryos produced by in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) with non- or HA-binding sperm (HABS). For binding of sperm with HA, sperm incubated in 10 ${mu}ell$ drop containing HA (0.8 mg/ml)-agarose (0.8%) mixture for 15 min. IVF and ICSI with non- or HA-bound sperm examined with matured oocytes at 44 hr after in vitro maturation. Embryos were cultured in 50 ${mu}ell$ of NCSU 23 containing 0.5% BSA for 5 days and then in 50 ${mu}ell$ of NCSU 23 containing 10% FBS for 2 days. For the evaluation of chromosomal aneuploidies, chromosome 1 sub-metacentric specific probe was used in sperm and embryos by fluorescence in situ hybridization (FISH). The frequency of aneuploidy sperm for chromosome 1 was 6.25%. The significant differences following IVF and ICSI with non- or HA-bound sperm were not observed in blastocyst formation rates (18.6, 23.5, and 23.8%) and cell number (61.8 $\pm$ 12.5, 55.5 $\pm$ 7.3, and 59.3 $\pm$ 9.6). Moreover, the percentage of diploidy in 4-cell stage embryos was 57.1% (IVF), 68.8% (ICSI), and 76.3% (ICSI-HABS). These results suggest that HA-binding sites may be a material for selection of normal sperm for ICSI. Therefore HA selection of normal sperm may be reduce the loss to embryonic mortality prior to embryo transfer in pig.

• IMMUNE NETWORK
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• 제2권3호
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• pp.133-136
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• 2002

Background: The costimulatory molecule 4-1BB, a member of nerve growth factor receptor/tumor necrosis factor (NGFR/TNFR) super family, is involved in cell survival and death. Methods: In this study, female C57BL/6 ($H-2^b$) mice were used as a recipient, and DBA/2 ($H-2^d$) as a donor to assess a mixed lymphocyte reaction (MLR) and CTL response in vitro, and skin graft survival. IL-2, IFN level was measured by ELISA. Results: Mixed lymphocyte reaction (MLR) analysis showed that 4-1BB-deficient responder cells showed enhanced cellular proliferation over littermate controls. In contrast, IL-2 production was diminished only in 4-1BB knockout cultures. The IFN expression, on the other hand, was comparable between the groups. When female C57BL/6 ($H-2^b$) mice were grafted with the trunk skin of DBA/2 ($H-2^d$) mice, the in vivo tissue destruction of 4-1BB-deficient mice was not distinct from the normal littermates. Conclusion: These data suggest that 4-1BB is critical for the induction of alloreactive responses in vitro but 4-1BB alone could not change the course of skin rejection in vivo.

• Imaging Science in Dentistry
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• 제51권1호
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• pp.1-7
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• 2021

Purpose: The aim of this study was to assess artifacts generated in cone-beam computed tomography (CBCT) of 3 types of dental implants using 3 metal artifact reduction (MAR) algorithm conditions (pre-acquisition MAR, post-acquisition MAR, and no MAR), and 2 peak kilovoltage (kVp) settings. Materials and Methods: Titanium-zirconium, titanium, and zirconium alloy implants were placed in a dry mandible. CBCT images were acquired using 84 and 90 kVp and at normal resolution for all 3 MAR conditions. The images were analyzed using ImageJ software (National Institutes of Health, Bethesda, MD) to calculate the intensity of artifacts for each combination of material and settings. A 3-factor analysis of variance model with up to 3-way interactions was used to determine whether there was a statistically significant difference in the mean intensity of artifacts associated with each factor. Results: The analysis of all 3 MAR conditions showed that using no MAR resulted in substantially more severe artifacts than either of the 2 MAR algorithms for the 3 implant materials; however, there were no significant differences between pre- and post-acquisition MAR. The 90 kVp setting generated less intense artifacts on average than the 84 kVp setting. The titanium-zirconium alloy generated significantly less intense artifacts than zirconium. Titanium generated artifacts at an intermediate level relative to the other 2 implant materials, but was not statistically significantly different from either. Conclusion: This in vitro study suggests that artifacts can be minimized by using a titanium-zirconium alloy at the 90 kVp setting, with either MAR setting.

• Investigative Magnetic Resonance Imaging
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• 제17권2호
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• pp.133-143
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• 2013

목적 : 직결장암 조직의 자기공명영상과 고주파 초음파검사를 시행한 후 종양의 탐지와 침윤 깊이에 대해 각각의 영상 소견과 병리 소견을 비교하여 진단적 정확도를 두 영상 기기 간 비교하고, 자기공명영상의 경우 종양의 침윤 깊이를 주변 정상 조직과 가장 명확히 보여주는 펄스 연쇄 (pulse sequence)에 대해 알아보았다. 대상 및 방법 : 직결장암으로 절제술을 시행한 45명의 환자에서 얻은 45예의 제거된 종양 조직을 수조에 넣고 생리 식염수에 담가서 고주파수 (5-17 MHz)의 선형 탐촉자를 이용하여 초음파영상을 얻었으며 8-channel 두경부 코일에 넣어 자기공명영상을 얻었다. 이 연구에 대하여 기관감사위원회의 공지에 입각한 동의는 면제되었다. 자기공명영상은 정- 및 탈위상 경사에코 T1 강조영상, 급속스핀에코 T2 강조영상 및 이의 지방억제 영상, fast imaging employing steady-state acquisition (FIESTA)와 이의 지방억제영상, 확산강조영상 등 일곱 가지 펄스 연쇄를 시행하였다. 각 조직의 자기공명영상과 초음파영상 소견을 각각 독립적으로 종양의 탐지와 침윤 깊이에 대하여 두 명의 영상의학과 의사가 합의 하에 평가하였고 각각의 영상 소견을 병리 조직 소견과 비교하여 두 영상 기기 간 진단적 정확도를 비교하였다. 자기공명영상의 일곱 가지 펄스 연쇄 중에 종양의 침윤 깊이를 주변 정상 조직과 구분하여 명확히 보여주는 펄스 연쇄에 대해 알아보았다. 결과 : 직결장암 조직의 종양 탐지와 침윤 깊이를 평가하는데 있어 자기공명영상과 초음파의 진단적 정확도는 각각 91.1%와 86.7%로 높게 나타났다. 조기 직결장암의 경우 초음파 검사의 정확도는 87.5%, 자기공명영상 검사의 정확도는 75.0%로 나왔다. 두 영상 기기 간에 통계적으로 유의한 차이는 없었다 (p > 0.05). MR의 펄스 연쇄 중에 종양의 침윤 깊이를 주변 정상 조직과 구분하여 명확하게 보여주는 영상은 직결장암 및 조기 직결장암 모두 급속 스핀에코 T2 강조영상이었다. 결론 : 자기공명영상과 초음파 검사는 직결장암 조직의 종양 탐지와 침윤 깊이를 평가하는데 높은 진단적 정확도를 가지고 있으며, 자기공명영상의 급속스핀에코 T2 강조영상이 직결장암 조직의 종양 침윤 깊이를 평가하는데 가장 우수하였다.

• Nuclear Engineering and Technology
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• 제34권2호
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• pp.146-153
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• 2002

To radiolabel bioactive molecules, we synthesized $^{99m}$Tc-tricarbonyl precursor, [$^{99m}$Tc(CO)$_3$($H_2O$)$_3$]$^{+}$ with a low oxidation state ( I ). The [$^{99m}$Tc(CO)$_3$($H_2O$)$_3$]$^{+}$ was prepared by low pressure carbonylation (1 atm of CO) of [$^{99m}$Tc $O_4$)]$^{[-10]}$ in the presence of NaB $H_4$ resulting in higher than 98% of labeling yield and stability up to 8 hrs. We evaluated the characteristics of $^{99m}$Tc- tricarbonyl labeled bioactive molecules by carrying out in vitro and in vitro study. Prepared [$^{99m}$Tc(CO)$_3$($H_2O$)$_3$]$^{+}$ was then reacted with some ligands of significance in modem diagnostic nuclear medicine and some amino acids. Labeling yields were checked by HPLC and found to be usually high, excluding $^{99m}$Tc-tricarbonyl-MDP, -EDTMP and -mIBG. And the biodistribution properties of $^{99m}$Tc-tricarbonyl complexes applied in rabbit showed different appearance comparing with that of the $^{99m}$Tc-labeling by conventional means. From these results, we conclude that [$^{99m}$Tc(CO)$_3$($H_2O$)$_3$]$^{+}$ is a potential precursor for development of radiopharmaceuticals, especially for labeling of biomolecules.

• Parasites, Hosts and Diseases
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• 제39권1호
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• pp.67-76
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• 2001

This experiment was focused on the characterization of anti- Toxoplasma monoclonal antibodies (mAbs) and the effect of mAbs on the parasite invasion of mouse peritoneal macrophages. Twenty eight mAbs including M110, M556, R7A6 and M62l were characterized by Ab titer, immunoglobulin isotyping and western blot pattern. Antibody titer (optical density) of 4 mAbs. Ml 10. M556. R7A6 and M62l. were 0.53,0.67, 0.45 and 0.39 (normal mouse serum; 0.19) with the same IgGl isotypes shown by Enzyme-linked immunosorbent assay (ELISA). Western blot analysis showed that Ml 10. M556. R7A6 and M62l reacted with the 33 kDa (p30),31 kDa (p28),43 kDa and 36 kDa protein. Immuno-gold labelling of mAbs M110, M556, R7A6 and M621 reacted with the surface membrane, dense granules and parasitophorous vacuolar membrane (PVM) , rhoptries and cytoplasm of tachyzoite, respectively. For in vitro assay, preincubation of tachyzoties with four mAbs, Ml 10, M556, R7A6 and M62l resulted in the decrease of the number of infected macrophages (p < 0.05) and the suppression of parasite multiplication at 18 h post-infection. Four monoclonal antibodies including Ml 10 (SAGI) were found to have an important role in the inhibition of macrophage invasion and T. gondii multiplication in vitro, and these mAbs may be suitable for vaccine candidates, diagnostic kit and for chemotherapy.

• Restorative Dentistry and Endodontics
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• 제41권1호
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• pp.6-11
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• 2016

Objectives: The purpose of this in vitro study was to evaluate the accuracy of working length (WL) determination of four electronic apex locators (EALs), namely, Root ZX (RZX), Elements diagnostic unit and apex locator (ELE), SybronEndo Mini Apex locator (MINI) and Propex pixi (PIXI) using Stainless steel (SS) and nickel-titanium (NiTi) hand files. The null hypothesis was that there was no difference between canal length determination by SS and NiTi files of 4 EALs. Materials and Methods: Sixty extracted, single rooted human teeth were decoronated and the canal orifice flared. The actual length (AL) was assessed visually, and the teeth were embedded in an alginate model. The electronic length (EL) measurements were recorded with all four EALs using SS and NiTi files at '0.5' reading on display. The differences between the AL and EL were compared. Results: The results obtained with each EAL with SS and NiTi files were compared with AL. A paired sample t test showed that there was a statistical significant difference between EAL readings with SS and NiTi files for RZX and MINI (p < 0.05). The accuracy of RZX, ELE, MINI and PIXI within ${\pm}0.5 mm$ of AL with SS/NiTi files were 93.3%/70%, 90%/91.7%, 95%/68.3%, and 83.3%/83.3%, respectively. Conclusions: The results of this study indicate that Root ZX was statistically more accurate with NiTi files compared to SS files, while MINI was statistically more accurate with SS files compared to NiTi files. ELE and PIXI were not affected by the alloy type of the file used to determine WL.

• 대한소아치과학회지
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• 제27권1호
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• pp.24-31
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• 2000

새로운 레이저형광측정기술을 사용한 Diagnodent의 우식진단능력을 탐침을 사용하는 시진법 및 일반적 방사선사진촬영법과 비교하여 평가하기 위하여, 건전하거나 열구우식이 있는 발거된 대구치와 소구치 103개를 대상으로 위의 세 가지 방법의 검사를 각각 시행하고 그 성적을 비교하였다. 시진 및 방사선검사 성적이 증가할수록 Diagnodent성적도 함께 증가하는 분포를 나타내었다(P<0.01). 시진 성적과 Diagnodent 성적 간에는 피어슨 상관계수 0.676, 스피어만 순위상관계수 0.694의 상관성이 있었고, 방사선검사 성적과 Diagnodent 성적 간에는 각각 0.623, 0.658의 상관성이 있었다(P<0.01, 전체). Diagnodent 검사법은 민감도가 매우 높은 것으로 나타났으나, 가양성의 측정치가 많아 특이도가 낮게 나타났으며, 우식단계별 진단기준을 제시하기 위하여는 더 많은 연구가 필요하다고 평가되었다.

• Journal of Information Processing Systems
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• 제15권4호
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• pp.820-832
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• 2019

Estimation of accurate blood volume flow in ultrasound Doppler blood flow spectrograms is extremely important for clinical diagnostic purposes. Blood volume flow measurements require the assessment of both the velocity distribution and the cross-sectional area of the vessel. Unfortunately, the existing volume flow estimation algorithms by ultrasound lack the velocity space distribution information in cross-sections of a vessel and have the problems of low accuracy and poor stability. In this paper, a new robust ultrasound volume flow estimation method based on multigate (RMG) is proposed and the multigate technology provides detail information on the local velocity distribution. In this method, an accurate double iterative flow velocity estimation algorithm (DIV) is used to estimate the mean velocity and it has been tested on in vivo data from carotid. The results from experiments indicate a mean standard deviation of less than 6% in flow velocities when estimated for a range of SNR levels. The RMG method is validated in a custom-designed experimental setup, Doppler phantom and imitation blood flow control system. In vitro experimental results show that the mean error of the RMG algorithm is 4.81%. Low errors in blood volume flow estimation make the prospect of using the RMG algorithm for real-time blood volume flow estimation possible.

• The Journal of the Acoustical Society of Korea
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• 제22권2E호
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• pp.69-77
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• 2003

Correlations between acoustic properties and bone density have been investigated in bovine cancellous bone. Speed of sound (SOS), broadband ultrasonic attenuation (BUA), and broadband ultrasonic reflection (BUR) were measured in 10 defatted bovine cancellous bone specimens in vitro. SOS showed a significant correlation with the apparent density of the bone. A comparable correlation was observed between BUA and the apparent density. BUR was rather highly correlated with the apparent density. It was shown that BUR had a weak correlation with BUA and a significant correlation with SOS. This indicates that the parameter BUR can provide important information that may not be contained in BUA and SOS and, therefore, can be useful as an alternative diagnostic parameter of osteoporosis. As expected, a linear combination of all three ultrasonic parameters in a multiple regression model resulted in a significant improvement in predicting the apparent bone density.