• 분석과학
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• 제28권2호
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• pp.79-85
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• 2015

TLC (SiO₂) 상에서 3가지 유사 암페타민 류 아민 화합물과 3가지 유도체화 시약 사이의 반응으로부터 얻어진 아민유도체의 이미지 분석으로부터 암페타민 류 화합물에 대한 신속하고 경제적인 반정량적인 분석방법을 제시하기 위하여 선행 실험을 하였다. TLC상 직접적인(in situ, co-spot) 유도체화 반응의 적정화를 시도하였고 그 TLC 결과물들을 디지털카메라로 촬영하여 이미지를 얻은 후 2가지의 이미지 분석 프로그램(CP Atlas 2.0 및 ImageJ)에 적용하여 재현성(RSD; %)과 상관성(R²)을 각각 확인 및 비교하였다. 그 결과, 반응 조건에 대한 조정이 필요하였고(반응 온도 등) 각 시료별 2가지의 농도(0.5 mg/mL 및 0.01 mg/mL)에서 반응의 재현성은 0.69~5.50% 범위이었으며 반응(2 µL per spot)의 생성물과 아민의 농도에 대한 상관성은 0.1~0.005 mg/mL의 농도 구간에서 가장 높게 나타났다(R²> 0.9906). 또한, 두 가지 프로그램 모두 재현성과 직선성에 대한 유사한 결과를 나타내어 직접적인 유도체화 반응과 TLC 이미지에 대한 이들 프로그램을 사용한 분석이 아민 화합물에 대한 반정량적인 분석으로 사용될 수 있는 가능성을 보였다.

• Clinical and Experimental Reproductive Medicine
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• 제24권3호
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• pp.335-340
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• 1997

Fluorescence in situ hybridization (FISH) techniques allow the enumeration of chromosome abnormalities and from a great potential for many clinical applications. In order to produce quantitative and reproducible results, expensive tools such as a cooled CCD camera and a computer software are required. We have developed a Chromosome Image Processing System (Chips) using FISH that allows the detection and mapping of the genetic aberrations. The aim of our study, therefore, is to evaluate the capabilities of our original system using a black-and-white video camera. As a model system, three repetitive DNA probes (D18Z1, DXZ1, and DYZ3) were hybridized to variety different clinical samples such as human metaphase spreads and interphase nuclei obtained from uncultured peripheral blood lymphocytes, uncultured amniocytes, and germ cells. The visualization of the FISH signals was performed using our system for image acquisition and pseudocoloring. FISH images were obtained by combining images from each of probes and DAPI counterstain captured separately. Using our original system, the aberrations of single or multiple chromosomes in a single hybridization experiment using chromosomes and interphase nuclei from a variety of cell types, including lymphocytes, amniocytes, sperm, and biopsied blastomeres, were enabled to evaluate. There were no differences in the image quality in accordance with FISH method, fluorochrome types, or different clinical samples. Always bright signals were detected using our system. Our system also yielded constant results. Our Chips would permit a level of performance of FISH analysis on metaphase chromosomes and interphase nuclei with unparalleled capabilities. Thus, it would be useful for clinical purposes.

• KSBB Journal
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• 제17권1호
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• pp.80-87
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• 2002

유기물 제거뿐만 아니라 안정적으로 질소와 인의 동시 제거를 위한 순환식 생물막 반응기를 제작, 운전하여 최적의 운전 인자를 도출하고, 질소 제거의 텃 번째 단계인 질산화 및 뒤이은 탈질에 관여하는 미생물들의 군집 구조 분석을 수행하였다. 유기물 제거와 질소와 인의 동시 제거를 위한 순환식 생물막 반응기는 143일 동안 운전되었다. 이 결과 $COD_{cr},\;BOD_5$, SS의 경우 각각 88, 88, 97%의 평균 제거효율을 보였다 이 기간 중 질산화율은 약 96% 정도로 유입 ${{NH_4}^{+}}_{-}N$의 대부분이 제거됨을 보였다. 하지만 탈질율은 평균 45% 정도로 나타났다. 반응기로 유입되는 총 인의 경우 약 44%가 제거되었다. 질소제거의 첫 번째 단계인 질산화가 일어나는 호기성 반응조 내 질산화 미생물의 경우 FISH 관찰 결과, 주요 암모니아 산화균 및 아질산 산화균으로는 Nitrosomonas spp.와 Nitrospira sap.가 관찰되었다. 또한 탈질 반응이 일어나는 준혐기성 반응조에서는 Rhodobacter, Rhodovulum, Roseebacter 그리고 Paracoccus 속에 속하는 탈질 미생물들이 전체 미생물의 약 10~20% 정도를 차지하며 분포하였다.

• 자원환경지질
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• 제46권5호
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• pp.425-444
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• 2013

이 연구는 공기주입법(in-situ air sparging; IAS)이 결합된 증기추출법(vapor extraction; VE)을 이용한 실내 실험 연구로 비수용성액체 유기물(non-aqueous phase liquids; NAPLs)의 총유류계탄화수소(total petroleum hydrocarbons; TPHs), 트리클로에틸렌(trichloroethylene; TCE), 퍼클로로에틸렌(perchloroethylene; PCE), 벤젠(benzene), 톨루엔(toluene), 에틸벤젠(ethylbenzene) 및 크실렌(xylenes)을 제거하기 위한 목적으로 국내 만돌, 하전, 상암, 부산만내 해양 퇴적물들을 대상으로 실험 하였다. 만돌 퇴적물은 사질(sand; S) 특성(평균입도 1.789 ${\Phi}$), 하전은 모래실트(sandy Silt; sZ) 특성(평균입도 5.503 ${\Phi}$) 및 상암은 실트(Silt; Z) 특성(평균입도 5.835 ${\Phi}$)을 나타내었다. 그리고 부산지역 퇴적물은 점토(Clay; C) 특성(평균입도 8.528 ${\Phi}$)을 나타내었다. 48시간 동안 공기를 주입하지 않은 B1 (0 L/min) 컬럼에서 남아있는 TPHs는 만돌, 하전, 상암지역 및 부산지역 샘플퇴적물에서 각각 약 2,459, 6,712, 4,348, 14,279 ppm으로 분석 되었다. 그리고 만돌, 하전, 상암지역 및 부산지역 샘플퇴적물의 B2 (3 L/min)-B5 (5 L/min) 컬럼에서 TCE는 99.5-100.0% 제거되었으며, PCE는 93.2-100.0%까지 제거된 결과를 보였다. 입자크기에 따른 각 성분의 제거량과의 상관관계는 TCE, PCE, toluene, etylbenzene, xylene, BTEX에서 모두 0.90-0.99의 밀접한 상관계수를 보였다. 그러나 TPHs에서는 0.76, Benzene에서는 0.71의 낮은 상관계수를 보였다.

• 한국지리정보학회지
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• 제21권3호
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• pp.162-175
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• 2018

본 연구는 도시 열환경 문제를 개선하기 위해 UAV 영상 표면온도 자료를 이용하여 피복재질별 표면온도 특성을 분석하였다. 그리고 UAV 영상 표면온도를 유사한 시기에 측정된 현장 실측 표면온도와 비교하였다. UAV 영상과 실측 표면온도와 비교한 결과, 가장 큰 차이를 보이는 피복재질은 회색 콘크리트 지붕 재질로 약 $7.8^{\circ}C$로 나타났다. 우레탄은 $0.3^{\circ}C$ 차이로 가장 적었다. 산점도를 분석한 결과 설명력이 63.75%로 상관성이 높은 것으로 분석되었다. 표면온도가 가장 높은 재질은 금속지붕으로 $48.9^{\circ}C$로 나타났고, 우레탄($43.4^{\circ}C$), 회색 콘크리트 지붕($42.9^{\circ}C$) 순이었다. 표면온도가 낮은 재질은 나지($30.2^{\circ}C$), 수목 및 잔디($30.2^{\circ}C$), 흰색 콘크리트 지붕($34.9^{\circ}C$)이었다. UAV 영상 표면온도 자료는 피복재질의 열적특성을 정밀하게 분석 가능하였다. 향후, 실측자료와의 비교를 통해 UAV 영상의 정확성 검 보정과 위성영상과 연계하여 UAV 영상 자료의 활용성을 확대할 필요가 있다.

• Advances in Computational Design
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• 제4권2호
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• pp.141-153
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• 2019

This research is comprised of virtually simulating behavior while experiencing low gravity effects in advance of real world testing in low gravity aboard Zero Gravity Corporation's (Zero-G) research aircraft (727-200F). The experiment simulated a drill rig penetrating a regolith simulant. Regolith is a layer of loose, heterogeneous superficial deposits covering solid rock on surfaces of the Earth' moon, asteroids and Mars. The behavior and propagation of space debris when drilled in low gravity was tested through simulations and visualization in a leading dynamic simulation software as well as discrete element modeling software and in preparation for comparing to real world results from flying the experiment aboard Zero-G. The study of outer space regolith could lead to deeper scientific knowledge of extra-terrestrial surfaces, which could lead us to breakthroughs with respect to space mining or in-situ resource utilization (ISRU). These studies aimed to test and evaluate the drilling process in low to zero gravity environments and to determine static stress analysis on the drill when tested in low gravity environments. These tests and simulations were conducted by a team from Texas A&M University's Department of Construction Science, the United States Air Force Academy's Department of Astronautical Engineering, and Crow Industries

• 한국환경과학회지
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• 제17권1호
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• pp.97-105
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• 2008

This study examined the contaminated soils with an indicator of TPH using SVE (Soil Vapor Extraction) and biological treatments. Their results are as follows. Water content in the polluted soils slowly decreased from 15% during the initial experimental condition to 10% during the final condition. Purification of polluted soils by Bioventing system is likely to hinder the microbial activity due to decrease of water content. Removal rate of TPH in the upper reaction chamber was a half of initial removal rate at the 25th day of the experiment. The removal rate in the lower reaction chamber was 45% with concentration of 995.4 mg/kg. When the Bioventing is used the removal rate at the 14th day of the experiment was 53%, showing 7 day shortenting. Since the Bioventing method control the microbial activity due to dewatering of the polluted soil, SVE method is likely to be preferable to remove in-situ TPH. The reactor that included microbes and nutrients showed somewhat higher removal rate of TPH than the reactor that included nurtients only during experimental period. In general, the concentration showed two times peaks and then decreased, followed by slight variation of the concentration in low concentration levels. Hence, in contrast to SVE treatment, the biological treatment tend to show continuous repetitive peaks of concentration followed by concentration decrease.

• 한국농공학회지
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• 제40권2호
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• pp.112-122
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• 1998

Measurement of electrical resistivity in soils has been used for many years with purpose of estimating in situ porosity or density. Recently electrical resistivity has also been used as an indicator of soil contaimination. This paper presents the result of laboratory experiment to investigate the resistivity variation in contaminated sandy soils. The results can be used with the Cone Penetrometer Test (CPT) result to analyse ground condition. In the experiment, the water content and leachate concentration of soils were controlled by groundwater and leachate, and then the resistivity measurement was made with 'STING-R1' by Advanced Geosciences Inc. In the case of using groundwater, the resistivity was in the range of over 1000${\omega}{\cdot}m$, but in the case of using polluted water by leachate, the resistivity decreased significantly down to 10~ 100${\omega}{\cdot}m$ for the same moisture content. Also the resistivity varied according to the degree of saturatrion. Therefore, if soil is contaminated by leachate, the CPT with electrical resistivity sensor might be used to investigate the contamination status and plume migration. But exact component of leachate and the pollutant concentration are still hard to identify.

• 농업과학연구
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• 제40권1호
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• pp.13-18
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• 2013

Effect of electric pulse stimulation was tested on the fruit body formation of valuable mushroom Tricholoma matsutake in the field of natural habitat of this mushroom. After applying the electric stimulator to the specific area of pine forest we found that the treatment especially stimulated the fructification of T. matsutake. And the most valuable findings of our study was that only our treatment plots showed fruit body formation whereas the control plots and the whole natural habitats of our study area showed zero production of this mushroom during the same time. From the point of view of mushroom production, our experiment shows that fruit body production can be upgraded by using pulsed power as an electrical stimulation in the field of the natural habitat of this mushroom. These findings from our experiment confirm the effectiveness of the significance of pulsed power technology for the improvement of T. matsutake fruit body production in the natural habit of this mushroom.

• Ocean and Polar Research
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• 제43권3호
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• pp.193-203
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• 2021

In-situ experiment using ocean buoys is a direct ocean observation and has been playing an important role from the past to the present based on high reliability. The ocean buoy is operated more stable than before due to the technological development of communication (GPS, satellite, …) and equipment, but still moored buoys are not free from various accidents occurring in the ocean. Nevertheless, there is currently a lack of countermeasures or manuals about mooring accidents. Therefore, in this study, based on the experience of operating buoys conducted for many years, the advantages and disadvantages of ocean buoys according to size were analyzed. and legal procedures before and after buoy mooring were presented to enhance the use of buoys. And it is suggested to realize successful experiment by proposing considerations before mooring the buoy in preparation for an accident.