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Anti-inflammatory Metabolites of Agrimonia pilosa Ledeb. and Their Mechanism

  • Park, Mi Jin;Ryu, Da Hye;Cho, Jwa Yeoung;Kang, Young-Hwa
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.13-13
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    • 2018
  • The anti-inflammatory (INF) compounds (1-15) were isolated from Agrimonia pilosa Ledeb. (APL) by activity-guided isolation technique. The isolated compounds (1-15) were identified as quercetin-7-O-rhanmoside (1), apigenin-7-O-glycoside (2), kaempferol-7-O-glycoside (3), apigenin-7-O-[6"-(butyl)-glycoside] (4), querceitn (5), kaempferol (6), apigenin (7), apigenin-7-O-[6"-(pentyl)-glycoside] (8), agrimonolide (9), agrimonolide-6-O-glucoside (10), desmethylagrimonolide (11), desmethylagrimonolide-6-O-glucoside (12), luteolin (13), vitexin (14) and isovitexin (15). Flavonoids, compound 2, 3, 11, and 14-15 have been found in APL for the first time. Furthermore, two novel flavone derivatives, compound 4 and 8, have been isolated inceptively in plant. In the no cytotoxicity concentration ranges of $0-20{\mu}M$, nitric oxide (NO) production level of 1-15 was estimated in LPS-treated Raw 264.7 macrophage cells. The flavone aglycones, 7 (apigenin, $IC_{50}=3.69{\pm}0.34{\mu}M$), 13 (luteolin, $IC_{50}=4.62{\pm}0.43{\mu}M$), 6 (kaempferol, $IC_{50}=14.43{\pm}0.23{\mu}M$) and 5 (quercetin, $IC_{50}=19.50{\pm}1.71{\mu}M$), exhibited excellent NO inhibitory (NOI) activity in dose-dependent manner. In the structure activity relationship (SAR) study of apigenin-derivatives (APD), apigenin; Api, apigenin-7-O-glucoside; Api-G, apignenin-7-O-[6"-(butyl)-glycoside]; Api-BG and apignenin-7-O-[6"-(pentyl)-glycoside]; Api-P, from APL on INF activity was investigated. The INF mediators level such as NO, INF-cytokines, NF-KB proteins, iNOS and COX-2 were sharply increased in Raw 264.7 cells by LPS. When pretreatment with APD in INF induced macrophages, NOI activity of Api was most effective than other APD with $IC_{50}$ values of $3.69{\pm}0.77{\mu}M$. And the NOI activity was declined in the following order: Api-BG ($IC_{50}=8.91{\pm}1.18{\mu}M$), Api-PG ($IC_{50}=13.52{\pm}0.85{\mu}M$) and API-G ($IC_{50}=17.30{\pm}0.66{\mu}M$). The NOI activity of two novel compounds, Api-PG and Api-BG were lower than their aglycone; Api, but more effective than Api-G (NOI: Api-PG and Api-BG). And their suppression ability on INF cytokines such as $TNF-{\alpha}$, $IL-1{\beta}$ and IL-6 mRNA showed the similar tendency. Therefore, the anti-INF mechanism study of Api-PG and Api-BG on nuclear factor-kappa B ($NF-{\kappa}B$) pathway, representative INF mechanism, was investigated and Api was used as positive control. Api-BF was more effectively prevent the than phosphorylation of $pI{\kappa}B$ kinase (p-IKK) and p65 than Api-PG in Raw 264.7 cells. In contrast, Api-PG and Api-BG were not reduced the phosphorylation of inhibitor of kappa B alpha ($I{\kappa}B{\alpha}$). Moreover, pretreatment with Api-PG and Api-BG, dose-dependently inhibited LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) mRNAs and proteins in macrophage cells, and their expression were correlated with their NOI activity. Therefore, APL can be utilized to health promote agent associated with their AIN metabolites.

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A Study on the clarification of the name of Anapji(雁鴨池) and Anapbupyeong(雁鴨浮萍) - Focusing on Anapji through its Appearance in Poems(題詠詩) ('안압지'의 이름과 '안압부평(雁鴨浮萍)'의 의미 연구 - 안압지(雁鴨池) 제영시(題詠詩)를 중심으로 -)

  • Kang, seog-keun
    • (The)Study of the Eastern Classic
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    • no.68
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    • pp.145-171
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    • 2017
  • This study aims to clarify the meaning of the Anapji which was one of the scenic places of historical interest during Silla Dynasty and of the Anapbupyeong(雁鴨浮萍) which was one of the greatest seven miracles (Dong-do-chil-gue(東都七怪) through appearance of the Anapji in poems. Anapji lies its original meanings on Anhaji(安夏池) and then it was shortened to Anji(雁池), apji(鴨池) while Dongho(東湖) has also been called since it is located in the South of Gyeongju. As of July 2011, the Ministry of Culture officially renamed Anapji and Imhaejeon Hall Site(臨海殿址) as the palace of Crown Prince-Gyonegju Donggung(慶州 東宮) and Wolji(月池) (Historical Site No. 18) and Wolji became the current name. While pond was made during Munmu of Silla, the name Anapji did not exist. Kim Si-Seup (1465~1471) in the early Chosun period referred to the pond as Anhaji(安夏池) and its name started being called as Anapji in the Geography of Korea (Sinjeung dongguk yeoji seungnam,(新增東國輿地勝覽)(Jungjong 25, 1530). Though modern scholar states that it was being called Anapji since there are always many ducks and wild geese in the pond, they fails to present the literature evidences. The author found the line in the poem -Anapji composed by writer Kim Chul Woo, which means that a wild duck and water bird get in and off the pond on their own and the record saying which means Dong-ho(東湖) from the phrase of Dongho-saw-sar-gi(東湖書社記) of Lee Su In(李樹仁)(1739-1822)-Confucian Scholar of Gyeongju was created due to the fact that many wild geese and ducks are flying over. Based on the facts foresaid, the literature evidences for the name of Anapji are presented in the paper. Anapji is a combined word between the Anapbupyeong(雁鴨浮萍) which is a flooding weed and Dong-do-chil-gue(東都七怪). Dong-do-chil-gue is an ancient form of Modern Eight Scenic Views. Eight Scenic Views -Gyeongju Parlgyong is just well-known as scenic places without grotesque elements Dong-do-chil-gue had before. However, the author also found the passage of Ilseongnok: Records of Daily Reflections saying that Anapji has a clod that is as wide as rock(盤石) and the clod also has moneywort on, which are undulating in the wind and clarified how the Anapbupyeong became one of the one of the greatest-seven miracles Dong-do-chil-gue and why the Anapbupyeong frequently appears in the poems of many writers.

Wildfire Severity Mapping Using Sentinel Satellite Data Based on Machine Learning Approaches (Sentinel 위성영상과 기계학습을 이용한 국내산불 피해강도 탐지)

  • Sim, Seongmun;Kim, Woohyeok;Lee, Jaese;Kang, Yoojin;Im, Jungho;Kwon, Chunguen;Kim, Sungyong
    • Korean Journal of Remote Sensing
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    • v.36 no.5_3
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    • pp.1109-1123
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    • 2020
  • In South Korea with forest as a major land cover class (over 60% of the country), many wildfires occur every year. Wildfires weaken the shear strength of the soil, forming a layer of soil that is vulnerable to landslides. It is important to identify the severity of a wildfire as well as the burned area to sustainably manage the forest. Although satellite remote sensing has been widely used to map wildfire severity, it is often difficult to determine the severity using only the temporal change of satellite-derived indices such as Normalized Difference Vegetation Index (NDVI) and Normalized Burn Ratio (NBR). In this study, we proposed an approach for determining wildfire severity based on machine learning through the synergistic use of Sentinel-1A Synthetic Aperture Radar-C data and Sentinel-2A Multi Spectral Instrument data. Three wildfire cases-Samcheok in May 2017, Gangreung·Donghae in April 2019, and Gosung·Sokcho in April 2019-were used for developing wildfire severity mapping models with three machine learning algorithms (i.e., Random Forest, Logistic Regression, and Support Vector Machine). The results showed that the random forest model yielded the best performance, resulting in an overall accuracy of 82.3%. The cross-site validation to examine the spatiotemporal transferability of the machine learning models showed that the models were highly sensitive to temporal differences between the training and validation sites, especially in the early growing season. This implies that a more robust model with high spatiotemporal transferability can be developed when more wildfire cases with different seasons and areas are added in the future.

Comparative Analysis among Radar Image Filters for Flood Mapping (홍수매핑을 위한 레이더 영상 필터의 비교분석)

  • Kim, Daeseong;Jung, Hyung-Sup;Baek, Wonkyung
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.34 no.1
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    • pp.43-52
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    • 2016
  • Due to the characteristics of microwave signals, Radar satellite image has been used for flood detection without weather and time influence. The more methods of flood detection were developed, the more detection rate of flood area has been increased. Since flood causes a lot of damages, flooded area should be distinguished from non flooded area. Also, the detection of flood area should be accurate. Therefore, not only image resolution but also the filtering process is critical to minimize resolution degradation. Although a resolution of radar images become better as technology develops, there were a limited focused on a highly suitable filtering methods for flood detection. Thus, the purpose of this study is to find out the most appropriate filtering method for flood detection by comparing three filtering methods: Lee filter, Frost filter and NL-means filter. Therefore, to compare the filters to detect floods, each filters are applied to the radar image. Comparison was drawn among filtered images. Then, the flood map, results of filtered images are compared in that order. As a result, Frost and NL-means filter are more effective in removing the speckle noise compared to Lee filter. In case of Frost filter, resolution degradation occurred severly during removal of the noise. In case of NL-means filter, shadow effect which could be one of the main reasons that causes false detection were not eliminated comparing to other filters. Nevertheless, result of NL-means filter shows the best detection rate because the number of shadow pixels is relatively low in entire image. Kappa coefficient is scored 0.81 for NL-means filtered image and 0.55, 0.64 and 0.74 follows for non filtered image, Lee filtered image and Frost filtered image respectively. Also, in the process of NL-means filter, speckle noise could be removed without resolution degradation. Accordingly, flooded area could be distinguished effectively from other area in NL-means filtered image.

A Study on the Structure Characteristics of Planting Ground in Incheon International Airport, Korea (인천국제공항 식재기반 구조 및 토양특성 연구)

  • Lee, Seung-Won;Han, Bong-Ho;Lee, Kyong-Jae;Kwak, Jeong-In;Yeum, Jung-Hun
    • Journal of the Korean Institute of Landscape Architecture
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    • v.43 no.3
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    • pp.77-91
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    • 2015
  • This study aims to suggest adequate soil management through the analysis of physicochemical properties of soil in the planting grounds of Incheon International Airport, which was constructed on a massive land reclamation site. Study areas were 5 sites at the international business complex, the passenger terminal, the airport support complex, the free trade zone, and the access road. Soil profile analysis showed that 9 plots out of the 27 plots were hardpan and heterospere within 80cm from the soil surface. The earth laid on the ground was categorized as gravel based soil(4 plots), dredged soil from the sea bottom and mixed reclamation materials(2 plots), clay with poor permeability(3 plots) and waste construction material(1 plot). Average soil hardness was $11.5kg/cm^2$ and soil textures were sandy soil, sandy loam and loamy sand. Average soil pH was 6.7 and average organic matter content was 0.7%. Electrical conductivity was 0.0dS/m and exchangeable cation concentrations were $Ca^{2+}$ 3.4cmol/kg, $Mg^{2+}$ 1.5cmol/kg, $K^+$ 0.3cmol/kg and $Na^+$ 1.0cmol/kg. Average cation exchange capacity was 11.0cmol/kg. Although average figures in Solum mostly meet the landscape design criteria, properties of each soil layer showed various values sometimes over the limit. Base saturations were $Ca^{2+}$ 29.9%, $Mg^{2+}$ 13.3% and $K^+$ 3.7% for lower soil, $Ca^{2+}$ 33.3%, $Mg^{2+}$ 17.0% and $K^+$ 2.7% for mid-soil and $Ca^{2+}$ 32.6%, $Mg^{2+}$ 12.2% and $K^+$ 1.9% for upper soil. Exchangeable sodium percentages were 16.4% for lower soil, 7.5% for mid-soil and 4.7% upper soil. Sodium adsorption rates were 0.8 for lower soil, 0.3 for mid-soil and 0.2 for upper soil. Factors affecting to the vegetation growth were heterogeneity and poorness of solum, disturbance of dredged soils, high soil hardness including hardpan in the subsurface soil layer and shallow effective soil depth, high soil acidity, imbalance of base contents, low organic matter content and low available phosphate levels in the soil.

DEM Generation over Coastal Area using ALOS PALSAR Data - Focus on Coherence and Height Ambiguity - (ALOS PALSAR 자료를 이용한 연안지역의 DEM 생성 - 긴밀도와 고도 민감도 분석을 중심으로 -)

  • Choi, Jung-Hyun;Lee, Chang-Wook;Won, Joong-Sun
    • Korean Journal of Remote Sensing
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    • v.23 no.6
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    • pp.559-566
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    • 2007
  • The generation of precise digital elevation model (DEM) is very important in coastal area where time series are especially required. Although a LIDAR system is useful in coastal regions, it is not yet popular in Korea mainly because of its high surveying cost and national security reasons. Recently, precise DEM has been made using radar interferometry and waterline methods. One of these methods, spaceborne imaging radar interferometry has been widely used to measure the topography and deformation of the Earth. We acquired ALOS PALSAR FBD mode (Fine Beam Dual) data for evaluating the quality of interferograms and their coherency. We attempted to construct DEM using ALOS PALSAR pairs - One pair is 2007/05/22 and 2007/08/22, another pair is 2007/08/22 and 2007/10/22 with respective perpendicular baseline of 820 m, 312m and respective height sensitivity of 75 m and 185m at southern of Ganghwa tidal flat, Siwha- and Hwaong-lake over west coastal of Korea peninsula. Ganghwa tidal flat has low coherence between 0.3 and 0.5 of 2007/05/22 and 2007/08/22 pair. However, Siwha-lake and Hwaong-lake areas have a higher coherence value (From 0.7 and 0.9) than Ganghwa tidal area. The reason of difference coherence value is tidal condition between tidal flat area (Ganghwa) and reclaimed zone (Siwha-lake and Hwaong-lake). Therefore, DEM was constructed by ALOS PALSAR pair over Siwha-lake and Hwaong-lake. If the temporal baseline is enough short to maintain the coherent phases and height sensitivity is enough small, we will be able to successfully construct a precise DEM over coastal area. From now on, more ALOS PALSAR data will be needed to construct precise DEM of West Coast of Korea peninsular.