• Title/Summary/Keyword: In-Depth analysis

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ChIP-seq Analysis of Histone H3K27ac and H3K27me3 Showing Different Distribution Patterns in Chromatin

  • Kang, Jin;Kim, AeRi
    • Biomedical Science Letters
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    • v.28 no.2
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    • pp.109-119
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    • 2022
  • Histone proteins can be modified by the addition of acetyl group or methyl group to specific amino acids. The modifications have different distribution patterns in chromatin. Recently, histone modifications are studied based on ChIP-seq data, which requires reasonable analysis of sequencing data depending on their distribution patterns. Here we have analyzed histone H3K27ac and H3K27me3 ChIP-seq data and it showed that the H3K27ac is enriched at narrow regions while H3K27me3 distributes broadly. To properly analyze the ChIP-seq data, we called peaks for H3K27ac and H3K27me3 using MACS2 (narrow option and broad option) and SICER methods, and compared propriety of the peaks using signal-to-background ratio. As results, H3K27ac-enriched regions were well identified by both methods while H3K27me3 peaks were properly identified by SICER, which indicates that peak calling method is more critical for histone modifications distributed broadly. When ChIP-seq data were compared in different sequencing depth (15, 30, 60, 120 M), high sequencing depth caused high false-positive rate in H3K27ac peak calling, but it reflected more properly the broad distribution pattern of H3K27me3. These results suggest that sequencing depth affects peak calling from ChIP-seq data and high sequencing depth is required for H3K27me3. Taken together, peak calling tool and sequencing depth should be chosen depending on the distribution pattern of histone modification in ChIP-seq analysis.

Analysis of Types of Gather Drape with Visual Evaluation (시각적 평가에 의한 개더 드레이프 형상 분석)

  • Lee Myung-Hee;Jung Hee-Kyeong
    • Journal of the Korea Fashion and Costume Design Association
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    • v.7 no.1
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    • pp.33-40
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    • 2005
  • Gathering is method used to control fullness along a seam line. The purpose of this study was to investigate the relationship between the quantitative research and qualitative method; the effect of gather and the types of gather drape. The experimental design consists of four factors: (l) three kinds of different weight and thickness of fabrics (2) three kinds of stitch densities (3) five kinds of ratio of gathers (4) three kinds of grain directions. Therefore one hundred thirty five (135) samples were made. And utilized SPSS WIN 10.0 Package in data analysis. The results of this study were as follows; First, after frequency analysis, side height, hem line width, node depth, node count, node width accorded with these result data recording. Second, after correlation analysis, side height related with front statements. Side height and entire visual was negative correlation. Hem line width, node depth, node count with section statements was negative correlation but node width at section statements was positive correlation. Third, after $k^2$ analysis, front picture parts getting excellent evaluation were 1st side height, 3rd hem line width, 4th node depth, 3rd node count, 3rd node width. And section illustration parts getting excellent evaluation were 4th side height, 1st hem line width, 2nd node depth, 3rd node count, 4th node width.

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The measurement of p-n junction depth by SEM

  • Hur, Chang-Wu;Lee, Kyu-Chung
    • Journal of information and communication convergence engineering
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    • v.5 no.4
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    • pp.324-327
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    • 2007
  • In this paper, the p-n junction depth with nondestructive method by using scanning electron microscopy (SEM) is determined and conformed. By measuring the critical short circuit current on the p-n junction which induced by electron beam and calculating generation range, the diffusion depth can be obtained. It can be seen that values destructively measured by constant angle lapping and nondestructively by this study almost concur. As this result, it is purposed that diffusion depth of p-n junction can be easily measured by nondestruction. This nondestructive method can be recommended highly to the industrial analysis.

Analysis of errors on the depth perception through binocular disparity in integral imaging

  • Kim, Joo-Hwan;Kim, Yun-Hee;Lee, Byoung-Ho
    • 한국정보디스플레이학회:학술대회논문집
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    • 2006.08a
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    • pp.1322-1325
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    • 2006
  • Integral imaging is a three-dimensional display method which has full parallax and continuous viewpoints. However, we found an error between the depth expressed by integral imaging and the depth perceived by the observer through binocular disparity. We analyze the depth perception errors of the threedimensional image constructed by integral imaging.

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Statics variation analysis due to spatially moving of a full ocean depth autonomous underwater vehicle

  • Jiang, Yanqing;Li, Ye;Su, Yumin;Cao, Jian;Li, Yueming;Wang, Youkang;Sun, Yeyi
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.11 no.1
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    • pp.448-461
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    • 2019
  • Changes in gravity and buoyancy of a Full Ocean Depth Autonomous Underwater Vehicle (FOD-AUV) during its descending and ascending process must be considered very carefully compared with a Human Occupied Vehicle (HOV) or a Remotely Pperated Vehicle (ROV) whose activities rely on human decision. We firstly designed a two-step weight dropping pattern to achieve a high descending and ascending efficiency and a gravity-buoyancy balance at designed depth. The static equations showed that gravity acceleration, seawater density and displacement are three key aspects affecting the balance. Secondly, we try our best to analysis the gravity and buoyancy changing according to the previous known scientific information, such as anomaly of gravity acceleration, changing of seawater states. Finally, we drew conclusion that gravity changes little (no more than 0.1kgf, it is impossible to give a accurate value). A density-depth relationship at the Challenger Deep was acquired and the displacement changing of the FOD-AUV was calculated preciously.

Analysis on the cause inducing an uncorrected disparity and distorted depth information by the image distance in stereo camera system (상거리에 따른 시차량의 변화 및 깊이 정보의 왜곡에 대한 연구)

  • Lee, Kwang-Hoon;Kim, Dong-Wook;Kwon, Yong-Moo;Chang, Eun-Young;Kim, Sung-Kyu
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.11B
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    • pp.1320-1327
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    • 2009
  • In stereoscopy using stereo camera system, the representative factors inducing stereoscopic depth distortions have been reported such as the inter camera distance, the convergence angle and the depth resolution. The image distance is just known to us as a factor related in optical system. In a point of view of depth distortion, it will be a factor inducing a stereoscopic depth distortion. In this paper, we focused on the proof of our opinion that the image distance is one of the weighted factors inducing depth distortion under orthostereoscopic condition.

Study on the Power Consumption Characteristics of Korean Domestic Species in Peripheral Milling with Image Analysis Technique

  • Lee, Hyoung Woo;Kim, Byung Nam;Kim, Kyung Yong
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.5 s.133
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    • pp.38-44
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    • 2005
  • Peripheral milling is one of the most important wood machining processes in wood industry. Power consumption characteristics of twelve Korean domestic species in peripheral milling were investigated in this study. Image analysis technique was applied to extract proper data from the power consumption profiles. Average power consumption increased as cutting depth increased and specific cutting power decreased as cutting depth increased. However, no significant relationship could be found between density and power consumption and between cutting depth and surface roughness.

P-wave velocity analysis around BSR depth using surface and ocean bottom seismic data (탄성파 자료를 이용한 BSR 부근의 속도 분석)

  • Kim, Byoung-Yeop;Koo, Nam-Hyung;Yoo, Dong-Geun
    • 한국지구물리탐사학회:학술대회논문집
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    • 2007.06a
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    • pp.151-156
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    • 2007
  • In December 2006, 2D surface streamer and Ocean Bottom Seismometer (OBS) data were acquired in the Ulleung basin in Korea where strong Bottom Simulating Reflectors (BSR) were shown as a result of 2D and 3D multichannel (MCS) reflection survey. The aim of this study is to provide another reliable source for estimating P wave velocity around BSR depth using OBS data in addition to velocity information from 2D surface seismic data. Four OBSs were deployed and four 20-km shot lines which pass two OBSs respectively were designed. To derive P wave velocity profile, interactive interval velocity analysis using ${\tau}$-p trajectory matching method (Kumar, 2005) was used for OBS data and semblance analysis was used for surface data. The seismic profiles cross the OBS instruments in two different directions yield recordings for four different azimuths. This raised the confidence for the results. All velocity profiles in the vicinity of BSR depth of four OBS sites show almost definite velocity changes which we could consider as upper BSR and free gas layer. Making comparison between velocity from OBS and that from 2D seismic semblance velocity analysis gives consistency in result.

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Variation of Microbial Community Along Depth in Paddy and Upland Field (논과 밭 토양에서 토층간 미생물 군집의 차이)

  • Kim, Chan-Yong;Park, Kee-Choon;Yi, Young-Keun
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.2
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    • pp.139-143
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    • 2009
  • We examined the vertical distribution of specific microbial groups and the patterns of microbial community structure within the soil profile using phospholipid fatty acid (PLFA). Samples were collected from the soil surface down to 15 cm in depth from paddy and upland fields located in Daegu, Korea. The two fields have been fertilized with only chemical fertilizers N, P, K for 33 years. Principal component analysis of the PLFA signatures indicated that the composition of the soil microbial communities changed significantly with the cultivation practices and soil depth, suggesting that cultivation practices of paddy and upland fields had more significant influence on soil microbial community than the soil depth did. The soil microbial communities changed more drastically with soil depth in upland field than in paddy field, with making thicker soil surface in paddy field in terms of soil microbial community. The ratios of cyclopropyl/monoenoic precursors and total saturated/total monounsaturated fatty acids increased with soil depth, suggesting that the deeper soil horizons are more carbon-limited and anaerobic than surface soil. The community analysis using PLFAs as biomarkers revealed that Gram-positive bacteria and actinomycetes tended to increase in proportional abundance with increasing soil depth, while the abundance of Gram-negative bacteria and fungi were highest at the soil surface and substantially lower in the subsurface.

Frequency Analysis Using Bootstrap Method and SIR Algorithm for Prevention of Natural Disasters (풍수해 대응을 위한 Bootstrap방법과 SIR알고리즘 빈도해석 적용)

  • Kim, Yonsoo;Kim, Taegyun;Kim, Hung Soo;Noh, Huisung;Jang, Daewon
    • Journal of Wetlands Research
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    • v.20 no.2
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    • pp.105-115
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    • 2018
  • The frequency analysis of hydrometeorological data is one of the most important factors in response to natural disaster damage, and design standards for a disaster prevention facilities. In case of frequency analysis of hydrometeorological data, it assumes that observation data have statistical stationarity, and a parametric method considering the parameter of probability distribution is applied. For a parametric method, it is necessary to sufficiently collect reliable data; however, snowfall observations are needed to compensate for insufficient data in Korea, because of reducing the number of days for snowfall observations and mean maximum daily snowfall depth due to climate change. In this study, we conducted the frequency analysis for snowfall using the Bootstrap method and SIR algorithm which are the resampling methods that can overcome the problems of insufficient data. For the 58 meteorological stations distributed evenly in Korea, the probability of snowfall depth was estimated by non-parametric frequency analysis using the maximum daily snowfall depth data. The results of frequency based snowfall depth show that most stations representing the rate of change were found to be consistent in both parametric and non-parametric frequency analysis. According to the results, observed data and Bootstrap method showed a difference of -19.2% to 3.9%, and the Bootstrap method and SIR(Sampling Importance Resampling) algorithm showed a difference of -7.7 to 137.8%. This study shows that the resampling methods can do the frequency analysis of the snowfall depth that has insufficient observed samples, which can be applied to interpretation of other natural disasters such as summer typhoons with seasonal characteristics.