• 한국응용과학기술학회지
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• 제31권4호
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• pp.663-668
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• 2014

An in-vivo diagnosis of trace Mg(II) ion was performed using a low-cost and environment-friendly voltammetric method, using a graphite counter and reference electrodes and a fluorine-immobilized graphite working electrode, and clean deep seawater was used as an electrolyte solution. Under optimum conditions, the analytical working ranges attained microgram ranges, and a detection limit of $80.6ugL^{-1}$ was obtained using stripping voltammety with 60 sec accumulation time. Ex-vivo application was performed on fish liver and mice droppings. The developed techniques can be applicable to tumor cell analysis.

• Applied Microscopy
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• 제33권2호
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• pp.131-143
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• 2003

메뚜기 체내와 체외에서 혈구 분화경로를 광학현미경과 전자현미경으로 관찰하였다. 조혈기관에서 혈구의 형성은 망상세포에 둘러싸인 줄기세포에서부터 유래되었으며, 줄기세포에서부터 원시혈구, 무정형혈구, I형 과립혈구, II형 과립혈구, 소구혈구 및 편도혈구가 각각 분화되는 것을 확인하였다. 곤충배지에 배양된 조혈조직에서 각각 다른 형태의 혈구들이 분화되어 방출되었다. 그러나, 이들 혈구들의 유사분열상은 관찰되지 않았다. 배지의 조혈기관에서 분화된 세포들의 형태학적 특징들은 메뚜기 체내의 조혈기관에서 분화된 세포들과 같았다. 이와 같은 결과는 줄기세포가 각각의 서로 다른 혈구들로 직접 분화하는 것을 의미한다. 본 연구 결과 조혈기관의 줄기세포는 각각의 혈구로 직접 분화할 수 있는 기능을 가지고 있었으며, 체내와 체외에서 한번 형성된 순환 혈구는 다른 혈구의 형태로 변형되지 않았다. 메뚜기에서 순환혈구의 유지는 복부 등쪽 첫 번째 마디에서 여덟 번째 마디 사이의 익상근 위에 광범위하게 존재하고 있는 조혈기관에 전적으로 의존하였다.

• Journal of Veterinary Science
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• 제22권6호
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• pp.41.1-41.14
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• 2021

Background: Our previously prepared ceftiofur (CEF) hydrochloride oily suspension shows potential wide applications for controlling swine Streptococcus suis infections, while the irrational dose has not been formulated. Objectives: The rational dose regimens of CEF oily suspension against S. suis were systematically studied using a pharmacokinetic-pharmacodynamic model method. Methods: The healthy and infected pigs were intramuscularly administered CEF hydrochloride oily suspension at a single dose of 5 mg/kg, and then the plasma and pulmonary epithelial lining fluid (PELF) were collected at different times. The minimum inhibitory concentration (MIC), minimal bactericidal concentration, mutant prevention concentration (MPC), post-antibiotic effect (PAE), and time-killing curves were determined. Subsequently, the area under the curve by the MIC (AUC_0-24h/MIC) values of desfuroylceftiofur (DFC) in the PELF was obtained by integrating in vivo pharmacokinetic data of the infected pigs and ex vivo pharmacodynamic data using the sigmoid E_max (Hill) equation. The dose was calculated based on the AUC_0-24h/MIC values for bacteriostatic action, bactericidal action, and bacterial elimination. Results: The peak concentration, the area under the concentration-time curve, and the time to peak for PELF's DFC were 24.76 ± 0.92 ㎍/mL, 811.99 ± 54.70 ㎍·h/mL, and 8.00 h in healthy pigs, and 33.04 ± 0.99 ㎍/mL, 735.85 ± 26.20 ㎍·h/mL, and 8.00 h in infected pigs, respectively. The MIC of PELF's DFC against S. suis strain was 0.25 ㎍/mL. There was strong concentration-dependent activity as determined by MPC, PAE, and the time-killing curves. The AUC_0-24h/MIC values of PELF's DFC for bacteriostatic activity, bactericidal activity, and virtual eradication of bacteria were 6.54 h, 9.69 h, and 11.49 h, respectively. Thus, a dosage regimen of 1.94 mg/kg every 72 h could be sufficient to reach bactericidal activity. Conclusions: A rational dosage regimen was recommended, and it could assist in increasing the treatment effectiveness of CEF hydrochloride oily suspension against S. Suis infections.

• Restorative Dentistry and Endodontics
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• 제36권5호
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• pp.367-376
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• 2011

치아우식증의 조기 발견은 외과적 삭제를 피하면서, 적절한 치료를 할 수 있는 좋은 기회를 제공한다. 광간섭단층촬영술은 최근 각광받기 시작한 3차원 이미지 기술로서, 안과에서 광학적 생검의 목적으로 빈번히 이용되는 것을 필두로 다양한 의학 분야에 적용되어 왔고, 최근 초기 우식증의 발견에 전도유망하여 다양한 연구가 진행 중이다. 이 기술은 저 상관도 간섭계의 원리에 근거하고 있으며, 장점으로는 비침습적이고, 방사선을 사용하지 않으며, 3차원 이미지 구축이 가능하다는 점이다. 본 연구에서는 광간섭단층촬영술의 원리와 개략적인 개발 과정에 대한 기술과 함께 치아우식증에 관한 연구들에 대하여 고찰해 보았고, 이를 통해 이 기술의 응용 가능성을 확인하였다. 그럼에도 불구하고, 임상적인 유용성을 입증하기 위해서는 몇 가지 기술적 문제를 해결해야 하고, 보다 많은 생체 내 실험이 뒷받침되어야 할 것이다.

• IEIE Transactions on Smart Processing and Computing
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• 제6권2호
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• pp.76-84
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• 2017

This paper presents an efficient method for generating spatiotemporal images in order to detect leukocyte motion in microvessels. Leveraging the constraint that leukocytes move along the contour line of the blood vessel wall, our proposed method efficiently generates spatiotemporal images for leukocyte motion detection. To that end, translational motion caused by in vivo movement is first removed by a template matching method. Second, the blood vessel region is detected by an automatic threshold selection method in order to binarize temporal variance images. Then, the contour of the blood vessel wall is expressed via B-spline function. Finally, using the detected blood vessel wall's contour as an initial curve, the plasma layer for the most accurate position is determined in order to find the spatial axis via snake, and the spatiotemporal images are generated. Experimental results show that the spatiotemporal images are generated effectively through comparison of each step with three images.

• Toxicological Research
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• 제30권2호
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• pp.117-120
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• 2014

Uranium is toxic and radioactive traces of it can be found in natural water and soils. High concentrations of it in biological systems cause genetic disorders and diseases. For the in vivo diagnosis, micro and nano range detection limits are required. Here, an electrochemical assay for trace toxic uranium was searched using stripping voltammetry. Renewable and simplified graphite pencils electrode (PE) was used in a three-electrode cell system. Seawater was used instead of an electrolyte solution. This setup can yield good results and the detection limit was attained to be at $10{\mu}gL^{-1}$. The developed skill can be applied to organic liver cell.

• Rapid Communication in Photoscience
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• 제1권2호
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• pp.53-53
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• 2012

• 대한의용생체공학회:의공학회지
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• 제8권1호
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• pp.75-80
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• 1987

With the conventional CW Doppler velocity meter, bl-directional velocities cannot be separated. The new CW Doppler system uses quadrature detection and phase rotation to produce simultaneous independent audio and velocity signals for forward and reverse blood flow direction, is fabricated. Specially, this system shows that phase rotation method for flow direction separation provides easy and satisfactory feature. From in vivo blood flow measurement, we can easily differentiate typical artery flow from vein flow, and measure both velocity characteristics qualitatively.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.191.2-191
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• 1999

No Abstract, See Full Text

• Nuclear Engineering and Technology
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• 제54권6호
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• pp.2213-2220
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• 2022

The maximum dose delivery at the end of the beam range provides the main advantage of using proton therapy. The range of the proton beam, however, is subject to uncertainties, which limit the clinical benefits of proton therapy and, therefore, accurate in vivo verification of the beam range is desirable. For the beam range verification in spot scanning proton therapy, a prompt gamma detection system, called as gamma electron vertex imaging (GEVI) system, is under development and, in the present study, the performance of the GEVI system in spot scanning proton therapy was predicted with Geant4 Monte Carlo simulations in terms of shift detection sensitivity, accuracy and precision. The simulation results indicated that the GEVI system can detect the interfractional range shifts down to 1 mm shift for the cases considered in the present study. The results also showed that both the evaluated accuracy and precision were less than 1-2 mm, except for the scenarios where we consider all spots in the energy layer for a local shifting. It was very encouraging results that the accuracy and precision satisfied the smallest distal safety margin of the investigated beam energy (i.e., 4.88 mm for 134.9 MeV).