• Journal of Microbiology and Biotechnology
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• 제33권12호
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• pp.1692-1697
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• 2023

Staphylococcus aureus integrated with mecA gene, which codes for penicillin-binding protein 2a, is resistant to all penicillins and other beta-lactam antibiotics, resulting in poor treatment expectations in skin and soft tissue infections. The development of a simple, sensitive and portable biosensor for mecA gene analysis in S. aureus is urgently needed. Herein, we propose a dual-toehold-probe (sensing probe)-mediated exonuclease-III (Exo-III)-assisted signal recycling for portable detection of the mecA gene in S. aureus. When the target mecA gene is present, it hybridizes with the sensing probe, initiating Exo III-assisted dual signal recycles, which in turn release numerous "3" sequences. The released "3" sequences initiate catalytic hairpin amplification, resulting in the fixation of a sucrase-labeled H2 probe on the surface of magnetic beads (MBs). After magnet-based enrichment of an MB-H1-H2-sucrase complex and removal of a liquid supernatant containing free sucrase, the complex is then used to catalyze sucrose to glucose, which can be quantitatively detected by a personal glucose meter. With a limit of detection of 4.36 fM for mecA gene, the developed strategy exhibits high sensitivity. In addition, good selectivity and anti-interference capability were also attained with this method, making it promising for antibiotic tolerance analysis at the point-of-care.

• 통상정보연구
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• 제15권3호
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• pp.289-310
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• 2013

지난 시기 우리나라는 수출진흥을 위한 정책적 수단으로 무역금융제도를 적극적으로 활용해 왔으며, 또한 그 효과에 있어서 의심의 여지가 없다. 그러나 2008년 리먼브라더스 파산으로 인한 세계금융위기를 겪으면서 국제사회는 유동성 리스크를 효과적으로 관리하기 위하여 바젤 III를 새롭게 등장시켰다. 우리나라는 시중은행을 중심으로 무역금융을 지원하고 있으나, 국내 시중은행의 취약한 해외신용평가, 위험인수 역량으로 인해 다양한 무역금융기법의 활용이 부족한 실정이다. 이러한 상황에서 바젤 III 등 관련 국제규범 도입에 따른 무역금융 위축 가능성에도 대비해야 한다. 따라서 본 연구에서는 우리나라 무역금융제도의 현황을 분석하고, 금융시장의 국제적 규제 강화 추이에 적극적인 대응방안을 모색하고자 한다. 이를 위해 기존의 시중은행을 통한 저금리 융자를 통한 지원정책을 벗어나 공공부문을 통한 무역금융지원이 확대되어야 한다. 특히 수출입은행, 한국무역보험공사 등을 적극 활용하여 해외 진출기업에 대한 지원, 고 신용위험 국가와의 교역에 대한 지원, 중소기업의 지원확대, 바젤 III 등 국제적 논의 참여 방안에 대하여 제시하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권1호
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• pp.361-365
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• 2012

Aims: To investigate the relationship between the expression of ${\beta}$-tubulin III and survivin in advanced breast cancers and chemotherapeutic effects of docetaxel. Methods: Clinical pathological data of 74 patients with advanced breast cancer were retrospectively analyzed after docetaxel chemotherapy. Expression of ${\beta}$-tubulin III and survivin was assessed by immunohistochemistry and analyzed with reference to therapeutical and adverse effects of docetaxel. Results: The positive expression rate of ${\beta}$-tubulin III was 38.1% (32/84), while that of survivin was 76.2% (64/84). The effective rate (complete response + partial response) was 52.4%. That for patients with the positive expression of ${\beta}$-tubulin III or/and survivin was significantly lower than for those with negative expression (P<0.05). There were significant differences in the non-progression of median diseases, 1-year and 2-year survival rates of between the patients with positive and negative expression (P<0.05). The main side effects were myelosuppression, alimentary canal response and alopecie, no differences being observed between groups. Conclusions: The combined detection of ${\beta}$-tubulin III and survivin is a predictive index for chemotherapy effects of docetaxel in metastatic breast cancer.

• The Korean Journal of Pain
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• 제13권2호
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• pp.175-181
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• 2000

Background: Pain is often measured using psychophysical scaling techniques. However, all of these methods found their limits, since they were based on the subjective sensations reported by the subjects. It is, therefore, desirable to validate psychophysical pain measures by simultaneously measuring some physiological correlate of nociception. We studied an objective method for measuring pain in human volunteer using R(III) nociceptive flexion reflex. Methods: Four different intensity of electrical stimuli between perception and 1.4 times the R(III) nociceptive flexion threshold were delivered to the sole of the feet in 8 normal volunteers. We measured the flexion reflex activity in the skin over the ipsilateral tibialis anterior muscle and subjects rated each stimulus on a visual analog scale (VAS) Results: Both R(III) nociceptive flexion reflex activity and VAS ratings showed a linear relationship with stimulus intensity and with each other in all volunteers. Conclusions: R(III) nociceptive flexion reflex elicited through electrical stimulation may used as an objective pain measurement, previsionary based on our study paradigm.

• Journal of Microbiology and Biotechnology
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• 제16권7호
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• pp.1132-1138
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• 2006

Three types of xylanases (EC 3.2.1.8) were detected in the strain Aspergillus niger A-25, one of which, designated as XynIII, also displayed ${\beta}-(l,3-1,4)-glucanase$ (EC 3.2.1.73) activity, as determined by a zymogram analysis. XynIII was purified by ultrafiltration and ion-exchange chromatography methods. Its apparent molecular weight was about 27.9 kDa, as estimated by SDS-PAGE. The purified XynIII could hydrolyze birchwood xylan, oat spelt xylan, lichenin, and barley ${\beta}-glucan$, but not CMC, avicel cellulose, or soluble starch under the assay conditions in this study. The xylanase and ${\beta}-(l,3-1,4)-glucanase$ activities of XynIII both had a similar optimal pH and pH stability, as well as a similar optimal temperature and temperature stability. Moreover, the effects of metal ions on the two enzymatic activities were also similar. The overall hydrolytic rates of XynIII in different mixtures of xylan and lichenin coincided with those calculated using the Michaelis-Menten model when assuming the two substrates were competing for the same active site in the enzyme. Accordingly, the results indicated that XynIII is a novel bifunctional enzyme and its xylanase and ${\beta}-(l,3-1,4)-glucanase$ activities are catalyzed by the same active center.

• Bulletin of the Korean Chemical Society
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• 제32권12호
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• pp.4321-4326
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• 2011

A new series of heteroleptic cyclometalated iridium(III) complexes has been synthesized and characterized by absorption, emission and cyclic voltammetry studies: $(pqx)_2Ir(acac)$ (1), $(dmpqx)_2Ir(acac)$ (2) and $(dfpqx)_2Ir(acac)$ (3) where pqx=2-phenylquinoxalinate, dmpqx=2-(2,4-dimethoxyphenyl)quinoxalinate, dfpqx=2-(2,4-difluorophenyl) quinoxalinate and acac=acetylacetonate anion. The reaction of excess acetylacetone with ${\mu}$-chloride-bridged dimeric iridium complex, $[(C\^N)_2Ir({\mu}-Cl)]_2$, gives a complex 1 and an unusual hydridoiridium(III) complex, $(pqx)IrH(acac)_2$ (4). The complex 1, 2 and 3 show their emissions in an orangered region (${\lambda}_{PL,max}$ = 583-616 nm), and the emission maxima can be tuned by the change of substituent at phenyl ring of 2-phenylquinoxaline ligand. The phosphorescent line shape indicates that the emissions originate predominantly from $^3MLCT$ states with little admixture of ligand-based $^3({\pi}-{\pi}^*)$ excited states. The structures of complex 3 and 4 are additionally characterized by a single crystal X-ray diffraction method. The complex 3 shows a distorted octahedral geometry around iridium(III) metal ion. A strong trans influence of the phenyl ring is examined. In complex 4, there are two discrete molecules which are mirror images each other at the ratio of 1:1 in an unit cell. We propose that the phosphorescent complex 1, 2 and 3 are possible candidates for the phosphors in OLEDs applications.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2002년도 춘계학술대회논문집
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• pp.441-447
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• 2002

KSR-III(Korea Sounding Rocket - III), which is being developed by Space Technology R&D Division of KARI(Korea Aerospace Research Institute) will be launched in late 2002. It is a three-stage, liquid propellant rocket which can reach 250 km altitude and will carry out observation of ozone layer and scientific experiments, such as microgravity experiment, and atmospheric measurement. KSR-III is believed to be an intermediate to the launch vehicle capable of carrying a satellite to its orbit. Space Test Department of KARI performed GVT(Ground Vibration Test) fer KSR-III EM at Rocket Test Building of KARI. GVT is very important for predicting the behavior of rocket in its operation, developing flight control program and performing aerodynamic analysis. This paper gives an introduction of rocket GVT configuration and information on test procedures, techniques and results of It. In this test. to simulate free-free condition, test object hung in the air laterally by 4 bungee cords specially devised. For the excitation of test object, pure random signal by two electromagnetic shakers was used and total 22 frequency response functions were achieved. Polyreference parameter estimation was performed to identify the modal parameters with MIMO(Multi-Input-Multi-Output) method. As the result of the test, low frequency mode shapes and modal parameters below 60Hz were identified

• Bulletin of the Korean Chemical Society
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• 제31권12호
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• pp.3617-3622
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• 2010

The reaction of stoichiometric amount of $FeCl_2{\cdot}4H_2O$, (2-pyridylmethyl, 3-pyridylmethyl)amine (2,3-pyma) and sodium azide/sodium thiocyanate in methanol under aerobic conditions affords the dinuclear Fe(III) complexes, [(2,3-pyma) $(N_3)_2Fe({\mu}-OCH_3)_2Fe(N_3)_2$(2,3-pyma)]${\cdot}CH_3OH$ (1) and [(2,3-pyma)$(NCS)_2Fe({\mu}-OCH_3)_2Fe(NCS)_2$(2,3-pyma)] (2) in good yield. Two bis-methoxy-bridged diiron(III) complexes are isolated and characterized. The coordination geometries around iron(III) ions in 1 and 2 are the same tetragonally distorted octahedron. The iron(III) ions are coordinated by two nitrogens of a 2,3-pyma, two nitrogens of two azide/thiocyanate ions, and two oxygens of two methoxy groups. Both compounds are isomorphous. The structures of 1 and 2 display the C-$H{\cdots}\pi$ and/or $\pi-\pi$ stacking interactions as well as hydrogen bonding interactions, respectively. Compounds 1 and 2 show significant antiferromagnetic couplings through the bridged methoxy groups between the iron(III) ions in the temperature range from 5 to 300 K ($H=-2JS_1{\cdot}S_2$, J=-19.1 and $-13.9\;cm^{-1}$ for 1 and 2).

• 자원리싸이클링
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• 제18권1호
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• pp.30-37
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• 2009

염산농도 0.001에서 10 M사이의 범위에서 백금(IV)과 팔라듐(II) 및 로듐(III)의 착물형성반응과 물질수지를 고려하여 각 금속의 분배곡선을 구했다. 염산농도가 0.1 M이상인 조건에서는 백금과 팔라듐의 대부분은 $PtCl_6^{2-}$와 $PdCl_2^{2-}$로 존재하였다. 로듐의 농도분포는 염산농도에 큰 영향을 받는다. 염산농도가 0.1에서 10 M로 증가함에 따라 로듐의 주 화학종이 $PhCl_5^{2-}$에서 $PhCl_6^{3-}$로 변했다. 문헌에 발표된 백금과 팔라듐의 용매추출자료로부터 $PtCl_6^{2-}$ 및 $PtCl_4^{2-}$와 수소이온간의 매개변수를 추산했다.

• Advances in environmental research
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• 제5권1호
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• pp.1-18
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• 2016

Magnetic nano-based sorbents have been synthesized for the recovery of two rare earth elements (REE: Nd(III) and Yb(III)). The magnetic nano-based particles are synthesized by a one-pot hydrothermal procedure involving co-precipitation under thermal conditions of Fe(III) and Fe(II) salts in the presence of chitosan. The composite magnetic/chitosan material is crosslinked with epichlorohydrin and modified by grafting alanine and serine amine-acids. These materials are tested for the binding of Nd(III) (light REE) and Yb(III) (heavy REE) through the study of pH effect, sorption isotherms, uptake kinetics, metal desorption and sorbent recycling. Sorption isotherms are well fitted by the Langmuir equation: the maximum sorption capacities range between 9 and 18 mg REE $g^{-1}$ (at pH 5). The sorption mechanism is endothermic (positive value of ${\Delta}H^{\circ}$) and contributes to increase the randomness of the system (positive value of ${\Delta}S^{\circ}$). The fast uptake kinetics can be described by the pseudo-second order rate equation: the equilibrium is reached within 4 hours of contact. The sub-micron size of sorbent particles strongly reduces the contribution of resistance to intraparticle diffusion in the control of uptake kinetics. Metal desorption using acidified thiourea solutions allows maintaining sorption efficiency for at least four successive cycles with limited loss in sorption capacity.