• 한국식품위생안전성학회지
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• 제15권2호
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• pp.128-136
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• 2000

수입 냉동어패류에서 24주의 Vibiro 균주를 순수 분리하여 그들의 생리, 생화학적 특성에 따라 동정한 결과 V. cholerae non-O1과 V. diazotrophicus가 각 2주, V. hollisae 1주, V. natriegens 5주, V. fluvialis 8주, V. nereis 4주인 것으로 밝혀졌고 2주는 Vibirio속 균주가 아닌 것으로 나타났다. 이 균주들을 API-2OE kit로 동정한 결과는 위의 결과와 매우 달라, V. cholerae로 동정된 2주와 fluvialis로 동정된 5균주의 경우에만 결과가 일치하였고 나머지는 Vibrio속이 아닌 것으로 분석되었다. 식품류에서 V. cholerae가 검출된 점에 주목하여 이 분리균들의 동정을 보다 정확히 하기 위하여 분리균들의 165 rRNA를 증폭시켜 이들의 RFLP를 분석하였다. 그 결과, r cholerae로 동정된 두 균주는 동일한 RFLP양상을 갖고 있었으며, V. cholerae보다는 V. proteolyticus의 RFLP에 보다 가까운 것으로 나타났다. 따라서 식품류의 병원균 검색을 보다 정확하고 신속하게 할 수 있도록 효율적인 검색 방법의 개발이 시급하다고 하겠다.

• 동아시아식생활학회지
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• 제18권6호
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• pp.873-883
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• 2008

In this study, we assessed consumers' recognition of frozen foods via a survey study, and monitored the contamination levels of total aerobic bacteria and Escherichia coli in imported and domestic frozen seafoods obtained from five whole sale markets in Seoul. A questionnaire used to assess the perception of frozen food safety and the attitude towards frozen food usage was developed and distributed to 350 adults. A total of 324 questionnaires were subjected to frequency analysis and a chi-square test, using SPSS for Windows. The results of our survey study demonstrated that 44.6% of the respondent consumed frozen processed foods two to three times per month, with dumplings being the most frequently purchased. 70.5% of the respondents selected "convenient cooking" as the principal reason for their frozen food purchases. 58% of the respondents believed that frozen processed food is not safe to eat as the result of food additives and changes in quality during the shelf life period. Consumers most profoundly preferred frozen seafood originating from America, and preferred that from China least profoundly (81.2%). Microbiological analyses demonstrated that the aerobic plate counts of frozen seafood, regardless of origin, fulfill the standard requirements except for one frozen clam (6.6 Log CFU/g), which was a heated-frozen domestic product. In addition, E. coli was isolated from frozen cooked mussels, frozen raw clams and squids, thus indicating that more strict hygienic regulation for frozen seafoods will be necessary to protect the consumer in both domestic and foreign markets.