• Asian-Australasian Journal of Animal Sciences
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• v.13 no.2
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• pp.236-243
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• 2000

A number of developments have occurred over recent years that are being used commercially or have the potential to increase the milk yield and consequently the efficiency of dairy cows. Bovine growth hormone is the most widely known of several attempts that have been made to alter the metabolic endocrinology of dairy cows to increase the rate of milk secretion. The factors affecting the milk yield response to growth hormone, growth hormone-releasing factor, thyroxine and placental lactogen as well as to the immuno-neutralization of somatostatin are briefly considered. Secondly, the recent greater understanding of the mechanism by which the milk yield is increased following more frequent milking, which has resulted from the identification and characterization of the feedback inhibitor of lactation (FIL) protein, is reviewed. The identification of this protein provides new avenues of research which may lead to a reduction in the rate of decline in milk yield with advancing lactation or to undiminished milk yields despite a reduction in frequency with which the animals are milked.

• Journal of Veterinary Science
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• v.22 no.4
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• pp.56.1-56.10
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• 2021

Background: Fluorescent antibody virus neutralization (FAVN) test is a standard assay for quantifying rabies virus-neutralizing antibody (VNA) in serum. However, a safer rabies virus (RABV) should be used in the FAVN assay. There is a need for a new method that is economical and time-saving by eliminating the immunostaining step. Objectives: We aimed to improve the traditional FAVN method by rescuing and characterizing a new recombinant RABV expressing green fluorescent protein (GFP). Methods: A new recombinant RABV expressing GFP designated as ERAGS-GFP was rescued using a reverse genetic system. Immuno-fluorescence assay, peroxidase-linked assay, electron microscopy and reverse transcription polymerase chain reaction were performed to confirm the recombinant ERAGS-GFP virus as a RABV expressing the GFP gene. The safety of ERAGS-GFP was evaluated in 4-week-old mice. The rabies VNA titers were measured and compared with conventional FAVN and FAVN-GFP tests using VERO cells. Results: The virus propagated in VERO cells was confirmed as RABV expressing GFP. The ERAGS-GFP showed the highest titer (10^8.0 TCID₅₀/mL) in VERO cells at 5 days post-inoculation, and GFP expression persisted until passage 30. The body weight of 4-week-old mice inoculated intracranially with ERAGS-GFP continued to increase and the survival rate was 100%. In 62 dog sera, the FAVN-GFP result was significantly correlated with that of conventional FAVN (r = 0.95). Conclusions: We constructed ERAGS-GFP, which could replace the challenge virus standard-11 strain used in FAVN test.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.3
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• pp.337-340
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• 1996

The role of endogenous somatostatin on lactation in rats was examined by passive immuno-neutralization of Wistar rats. In one study, the rats were given either immunoglobulin raised in sheep against somatostatin, or non-specific sheep immunoglobulins by daily s.c. injection from parturition through the first two weeks of lactation. The growth of the pups was recorded by weighting every second day, and the milk yield calculated from the pup weight and weight gain. Immunoneutralization of maternal somatostatin during pregnancy had a slight effect (p < 0.05) on the mean birth weight of the pups but no subsequent effect on postnatal growth rate of the pups or milk yield ($25.32{\pm}0.88g/day$) compared with young control rats given normal sheep serum ($25.55{\pm}1.04g/day$). Similarly, passive immunization against somatostatin during lactation ($21.96{\pm}1.57g/day$) also did not affect milk yields compared with controls ($24.85{\pm}1.03g/day$). These data do not support a significant role for endogenous somatostatin in regulating milk production in lactating rats.

• The Korean Journal of Food And Nutrition
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• v.28 no.6
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• pp.1090-1097
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• 2015

Traditionally, mistletoe is known as an effective anti-cancer medicinal plant, and lectin is recognized as a major component with cytotoxic and immuno-stimulant activity in mistletoe. A Korean mistletoe lectin (KML) has specificity to galactose and galactosamine and is distinguish from European mistletoe lectin (EML). When we examined the concentration of lectin in mistletoe originated from five different types of host trees, the result indicate that the lectin concentration is variable depending on the host tree. Noticeably, mistletoe from chestnut tree contains ten folds higher lectins than that of an oak tree. We also tested the concentration of KML and crude extract (KM-110) of Korean mistletoe that shows 90% cytotoxicity in L5178Y-ML25 lymphoma cell. In addition, the cells show 90% and 70% viability by the treatment of two neutralizing antibodies of KML, 9H7-D10 and 8B11-2C5 neutralization effect with two monoclonal antibodies of KML, 9H7-D10 and 8B11-2C5. Therefore, the result expected that the mistletoe contain some other cytotoxic components except lectin. Finally, the production of $TNF-{\alpha}$ and IL-6 by RAW 264.7 cells stimulated with lectin free-crude extract (LFKM-110) following neutralization by 9H7-D10 monoclonal antibody shows higher than that of lectin containing-crude extract (KM-110). These results suggest that the Korean mistletoe lectin ha a great potential to be developed as therapeutic agent of cancer.

• Journal of Animal Science and Technology
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• v.51 no.5
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• pp.407-412
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• 2009

This study was performed to produce specific egg yolk antibodies against bovine rotavirus (BRV) and bovine coronavirus (BCV) that are major pathogens causing diarrhea in calves. Chickens were immunized with BRV and BCV intramuscularly in the breast muscle by injection 5 times at two weeks interval. At 6 weeks after the first immunization of BRV or BCV, cross reactivity of each serum derived from BRV- or BCV-immunized hens was tested. Each serum antibody against BRV or BCV was reacted with only specific BRV or BCV antigens. Serum and egg yolk-antibody titers of hens against BRV or BCVwere highest at 8~12 weeks after first immunization. Specific serum and egg yolk-antibody titers against BRV were about 104,000 and 107,000, respectively, and those against BCV were about 145,000 and 155,000, respectively. Hemagglutination inhibition titers in the immunized egg yolk antibodies against BRV and BCV were 5,120 and 1,280, respectively, and were ${\geq}8$ times higher than that in non-immunized control. These results suggested that the immunized egg yolk antibodies could effectively neutralize BRV and BCV.