• The Korean Journal of Physiology and Pharmacology
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• 제22권1호
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• pp.81-89
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• 2018

This study evaluated the anti-asthmatic activities of 2,6-di-tert-butyl-4-hydroxymethylphenol (DBHP) that is a potent phenolic antioxidant in edible vegetable oil. The effects of DBHP on bronchial asthma were evaluated by determining the specific airway resistance (sRaw) and tidal volume (TV) during the immediate asthmatic response (IAR) and the late-phase asthmatic response (LAR) in guinea pigs with aerosolized ovalbumin-induced asthma. Recruitment of leukocytes and the levels of biochemical inflammatory mediators were determined in the bronchoalveolar lavage fluids (BALFs), and histopathological surveys performed in lung tissues. DBHP significantly inhibited the increased sRaw and improved the decreased TV on IAR and LAR, and also inhibited recruitment of eosinophils and neutrophils into the lung, and release of biochemical inflammatory mediators such as histamine and phospholipase $A_2$ from these infiltrated leukocytes, and improved pathological changes. However, anti-asthmatic activities of DBHP at oral doses of 12.5 to 50 mg/kg was less than those of dexamethasone (5 mg/kg, p.o.) and cromoglycate (10 mg/kg, p.o.), but more potent or similar to that of salbutamol (5 mg/kg, p.o.). These results in the present study suggest that anti-asthmatic effects of DBHP in the guinea pigs model of OVA-induced asthmatic responses principally are mediated by inhibiting the recruitments of the leukocytes and the release of biochemical inflammatory mediators from these infiltrated leukocytes.

• 동국한의학연구소논문집
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• 제2권2호
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• pp.57-68
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• 1993

In humans, an immediate allergic response to an allergen snay appear as either bronchial asthma unaccompanied by anaphylaxis or a severe bronchial constriction and spasm accompanied by anaphylaxis. Much experimental evidence with isolated asthmatic tissues has shown that histamine is released during the allergic reaction. Paradoxically, antihistamine treatment is ineffective in reversing bronchial asthma or anapllylaxis in a man. The present study objective is to search for protective agents(In-Sam-Yun-Pai-San-Ga-Gam-Bang) against bronchial constriction in treating a anaphylaxis. The result from this study is as follows. A single dose of In-Sam-Yun-Pai-San-Ga-Gam-Bang extract(3ml/kg body weight) 2 hrs prior to histamine-induced bronchial constriction test demonstrated significant protection. Based on the above result, It is considered that In-Sam-Yun-Pai-San-Ga-Gam-Bang can be used in bronchial asthma.