• Archives of Pharmacal Research
• /
• v.14 no.4
• /
• pp.352-356
• /
• 1991

The anti-platelet activity of ilexoside D isolated from the roots of Ilex pubescens Hook. et Arn. was investigated in in vitro and ex vivo models of platelet aggregation induced by ADP, thrombin or collagen in rats. In vitro ilexoside D inhibited more effectively platelet aggregation induced by ADP and thrombin than by collagen as compared with aspirin. Ex vivo ilexoside D also inhibited platelet aggregation induced by ADP and collagen, but not by thrombin, and the inhibitory action of ilexoside D was more effective than that of aspirin. However, in vitro ilexoside D inhibited very poorly the generation of malonyldialdehyde, which is known to be concomitantly released with thromboxane $A_2$ during platelet aggregation. These results suggest that the anti-platelet activity of ilexoside D may not be responsible for prostaglandin synthesis in platelets.

• Archives of Pharmacal Research
• /
• v.16 no.3
• /
• pp.209-212
• /
• 1993

The anti-coagulant activity of ilexoside D isolated from the roots of ilex pubescens Hook. et Am. was investigated in in vivo models of blood coagulation in rats. On oral administration, ilexoside D prolonged the bleeding time and the whole blood recalcified clotting time, but not the plasma recalcified clotting time. In vitor, ilexoside D did not affect the recalciffed clotting times of whole blood, platelet-rich plasma(PRP), and platelet-poor plasma(PPP), while in the presence of tissue factor the compound prologed the reduced proth-rombin times of whole blood, PRP and PPP in the dose-dependent manner. These results indicate that ilexoside D has the anit-tissue factor activity as well as the antithromobotic activity.

• Korean Journal of Pharmacognosy
• /
• v.33 no.4 s.131
• /
• pp.301-304
• /
• 2002

Two triterpenoid saponine were isolated from the stems of Acer ginnala Maxim. The structures of triterpenoid saponins were established as ilexoside O, $3-O-{\alpha}-L- rhamnopyranosyl(1{\rightarrow}2)-{\beta}-D-glucopyranosyl(1{\rightarrow}2)-{\beta}-D-xylopyranosyl-pubescenolic$ acid 28-{\beta}-D-glucopyranosyl$ ester(1) and ilexoside K, $3-O-{\beta}-D-glucopyranosyl(1{\rightarrow}2)-{\beta}-D-xylopyranosyl-pubes-cenolic$ acid $28-{\beta}-D-glucopyranosyl$ ester(2). Their chemical structures have been elucidated on the basis of spectral methods.

• Archives of Pharmacal Research
• /
• v.10 no.2
• /
• pp.115-120
• /
• 1987

Methanol extract of Ilex pubescens roots prolonged bleeding time threefold, and inhibited the generation of malondialdehyde released during platelet aggregation inducted by thrombin. Through several purification procedures, its saponin, named ilexoside, was proved to be responsible for the antithrombotic activities of the plant. Ilexosides A, -D and -J and 24-carboxypomolic acid showed strong inhibitory activities on platelet aggregation induced by thrombin.