• Journal of Pharmaceutical Investigation
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• 제33권4호
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• pp.287-292
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• 2003

A novel antitumor agent, antibody-endostatin fusion protein $(anti-HER2/neu\;IgG3C_H3-Endostatin,\;AEFP)$ formed by genetic engineering procedure from antibody (Ab) which specifically targets to tumor cells ad angiogenesis inhibitor, endostatin (Endo) that has excellent antitumor effect, minimizes the toxicity of normal cells and selectively kills only tumor cells. The purpose of this study is to evaluate the phamacokinetic parameters and to analyze the localization of AEFP. After an intravenous injection of $150\;{\mu}l\;(5\;{\mu}Ci)\;[^{125}I]Ab,\;[^{125}I]AEFP$ to mice, blood was collected though retroorbital plexus from 15 min to 2880 min. Following the jugular vein injetion of $150\;{\mu}l\;(10\;{\mu}Ci)\;[^{125}I]Endo$, blood was collected by the use of carotid artery cannulation from 0.25 min to 30 min. Consequently, Endo was very rapidly removed from plasma compartment within 30 min. On the other hand, AEFP similar to Ab was slowly cleared from plasma. Also, Endo was metabolized about 40% within 30 min. However, AEFP was shown to metabolize less than 10% within 2880 min. The organ distribution of Endo was in order kidney, lung, spleen. Both Ab and AEFP were localized in order spleen, kidney, liver. Futhermore the tumor/blood distribution ratio of AEFP at 96 hours after injection is about 20 times higher than it of Endo at one hour after injection. In conclusion, these studies demonstrate that the anti-cancer or suppression of angiogenesis effect of Endo may be improved by the use of AEFP because the longer half life and stability of AEFP is able to selectively target antigens expressed on tumors.

• 생약학회지
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• 제27권4호
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• pp.371-377
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• 1996

In previous studies we reported that the levels of RBC, hemoglobulin (HGB) and hematocrit (HCT) in SAM P6 were increased significantly from 7 day after oral administration of the pilose antler extract, 5 g/kg/day, and were lasted during the study. Thus, this study was performed to elucidate mechanism of erythropoietic action by the extract administration. SAM R1 and SAM P6 were chosen as experimental animals. At age of 12 weeks, pilose antler extract were given 0.3 and 5 g/kg/day (p.o.) each for 0, 7 and 14 days in both animals. Complete blood cells such as RBC, HGB, and HCT were counted. And plasma concentration of erythropoietin (EPO) which is the major regulator of erythropoiesis was measured using $^{125}I-antierythropoietin$ IgG. Total iron concentration in plasma was also analyzed. The levels of RBC and HCT were increased significantly after administration at both doses of 0.3 and 5 g/kg/day in SAM P6, however, these were increased only at dose of 5 g/kg/day in SAM R1. The plasma EPO concentration was increased significantly after administration in SAM P6. The plasma concentrations of total iron were significantly decreased after administration of the extract in SAM P6. These results suggest that the changes in erythropoietic effects after the administration of pilose antler extract may be mediated, at least in part, through the change in the plasma EPO.

• 한국통신학회논문지
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• 제16권2호
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• pp.115-127
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• 1991

본 논문은 한국의 ISDN 번호체계 질환에 대하여 검토한 것이다. ISDN 번호체계에서 NDC로써 사입자 구분. 서비스망 직별, 지역구분을 하고, 시외 10자리와 시내 7자리의 폐쇄식 번호체계를 구상하였다. 아울러 이동전화, 페이지, 팩스, 텔렉스, 컴퓨터 등의 전용망이나 별도의 공중망이라도 ISDN망에 접속할 수 있는 번호체계를 구상하였다. 특히 수도권과 영남권 분제와 통일후 남북통신의 구축에 대비해서 강남북 지역 구분 및 전국을 북부, 중부, 남부의 3등분 체계로 구성하였다. 그외 특수번호와 오디오텍스, 대내표용 전화등에 대하여 번호부여의 가능성을 제지, 구성하였다. 다만 ISDN에서 SA가 제외되었으며 정말 수요예측과 서비스 예측은 다루지 않았다. 결과적으로 NDC 3자리만으로도 ISDN 번호구성 배분이 가능함을 보였다.

• 한국전자파학회논문지
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• 제20권1호
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• pp.83-90
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• 2009

본 논문에서는 상호 평행하는 두 개의 도체 평판의 슬릿을 통한 전자파 투과 문제를 고려하였다. TM 편파된 평면파가 슬릿에 입사되었을 때 각 슬릿의 등가 자류를 미지수로 하는 결합 적분방정식을 유도하고 모멘트 방법으로 풀어 수치해석하였다. 슬릿의 폭, 도체 평행 평판의 간격, 평면파의 입사각, 슬릿간 변위 등에 따른 슬릿을 통해 투과된 전력의 변화를 계산하였다. 두 슬릿간 변위가 반 파장의 정수배에 근접할 때 투과 공진(최대 전력 투과)현상이 관찰되었다. 슬릿의 폭과 도체판 간격이 파장에 비해 매우 좁은 경우 투과 전력의 최대치는 슬릿의 폭과 입사각도에 무관하게 일정함을 확인하였다. 또한, 제안된 구조에서 일어나는 투과 공진 현상의 원리를 단순화된 구조와 등가회로를 통해 설명하였다.

• Journal of Nutrition and Health
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• 제29권10호
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• pp.1059-1071
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• 1996

This study was performed to investigate the effect of dietary protein level on renal senescence. Male rats of 337.8$\pm$5.7g body weight were underlateral nephrectomy or shamoperation. The rats were divided into high protein(40% casein), normal protein(15% casein) and low protein(8% casein)diets and fed experimental diets ad libitum for 24 weeks. The results are summarized as follows. There was a hypertophy of the remnant kidney of uninephrectomized rats of 40% or 15% protein group, coming up to the comparable weights of both kidneys of sham-operated rats. However, the hypertrophic effect was not seen in uninephrectomized rats of 8% protein group. Serum albumin was lower in uninephrectomized rats. With increasing dietary protein level blood urea nitrogen was increased, whereas, urinary urea nitrogen excretion was decreased. Urinary solute excretion was higher in uninephrectomized group than in sham-operated group. However, effect of dietary protein level on urinary solute excretion varied dpending on th solutes tested. GFR and urinary protein excretion, throughout experiment, increased with feeding period and with dietary protein level. Proteinuria was most severe in uninephrectomized rats fed 40% casein diet. Maximum urine concentration ability measured after dehydration was not different among the experimental groups. Light microscopic examination showed focal segmental glomerulosclerosis and mild increas of glomerular mesangial matrix in uninephrectomized rats fed 40% and 15% protein diet, however, which was not observed in uninephrectomized rats fed 8% protein diet and in sham-operated rats fed 40% diet. Immunofluorescence studies revealed segmental deposits of albumin in the mesangium and capillary loops in high protein and uninephrectomized groups. Minimal granular deposition of IgG was noted in the mesangium of all experimental groups. In conclusion, high protein intake accelerated deterioration of renal function and it was correlated with morphological change. Low protein intake was effective in preventing these changes.

• 동의생리병리학회지
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• 제16권4호
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• pp.693-700
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• 2002

SCRT (Sochungyong-tang) has been used for immune disease in human. The purpose of this study was effect of Helper T cell, major regulator of immune system. Spleen cell from 8 week BALB/c mice were cultured in SCRT containing medium without activation for 48 h. The MTS assay and flow cytometry revealed that SCRT treated Iympocyte were non-effect in percentage of CD4+ T cell. Subsequently CD4+ T cell were isolated and cultured in SCRT containing medium. SCRT were non-effective on CD4+ T cell without any involvement of APC. In order to evaluate the direct effect of SCRT on Helper T cell, CD4+ T cell isolated after 48 h of culture in SCRT containing medium and activated with and without anti-CD3/anti-CD28 activation for 48 h. A lower level of CD69 was observed in SCRT treated cells in flow cytometry analysis. Subsequently Using RT-PCR analysis the expression of mRNA for IL-2, INF-γ are upregulated and, IL-4 is downregulated in CD4 T cell. The result suggests that SCRT makes Th1 significantly increased and Th2 relatively inhibited. The results suggest that SCRT potentiate Th1 cell and decrease Th2 development at the same time, which is believed to be bemeficial for IgE-mediated responses.

• Toxicological Research
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• 제16권4호
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• pp.269-274
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• 2000

Epoxidised soya bean oil (ESBO, 1000, 2000 or 4000 mg/kg) was orally administered to BALB/c mice daily for 28 consecutive days, and the control mice were exposed to vehicle (corn oil). Mice were immunized and challenged with sheep red blood cells (SRBC) or bovine serum albumin (BSA). In groups exposed to ESBO, the body weight gains and the relative lymphoid organ weights were not significantly changed as compared with control group. Secondary IgG antibody response to BSA was not significantly changed by ESBO, but plaque-forming cell (PFC) response to SRBC was significantly suppressed in mice treated with 4000 mg ESBO/kg/day. The mitogenic response of splenic B cells induced by LPS was not effected by ESBO in any of the groups. These results indicate that ESBO did not induce significant humoral immune response at a dose less than 2000 mg/kg/day in mice.

• Archives of Plastic Surgery
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• 제32권4호
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• pp.529-532
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• 2005

Expanding cells ex-vivo is very important in tissue-engineering. Culture medium is usually supplemented with fetal bovine serum(FBS) in most of the experiments. However, cells grown in bovine serum media may posses the possibilities of disseminating bovine diseases and/or stimulating the patient's immune reactions. To overcome these problems, autologous or homologous serum should be used instead of the FBS. The purpose of this study is to compare cell proliferation and collagen synthesis depending on the kind of sera mixed on media and to provide a guideline on applying established experimental data to clinical cases. Human dermal fibroblasts were obtained from four patients. Five thousand cells per well in 96-well plates were incubated DMEM/F-12 Nutrient with varying serum mixture; 10% autologous serum, 10% homologous serum, and 10% FBS. Five days after incubation fibroblast proliferation and collagen production were determined by MTT assay and CICP enzyme immunoassay. The mean cell number were; $3.95{\times}10^4/well$, $2.97{\times}10^4/well$ and $2.30{\times}10^4/well$, respectively. The average amounts of collagen synthesized were; 238.13 ng/ml, 204.88 ng/ml, and 163.88 ng/ml in each. These results show that the use of human serum mixture may contribute to, not only preventing disseminated infection of bovine diseases. but also increase cell proliferation and collagen synthesis without simulating the patient's immune reactions.

• 한국수산과학회지
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• 제25권2호
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• pp.151-163
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• 1992

황해, 동지나해와 제주도 주변해역의 해수분포의 계절변화를 살펴본 결과 이 해역전반에 걸쳐 공통된 계절변화가 보였다. 고온고염의 해수는 겨울에 황해 쪽으로 확장되며 여름에 동지나해 쪽으로 후퇴한다. 순압모델결과는 계절풍이 이와 같은 해수분포의 계절변화를 유도할 수 있음을 보여준다. 역풍류는 이 과정에서 중요한 역할을 하는데, 모델결과에는 역풍류의 축이 중국 쪽으로 이동되어 나타난다. 역풍류 축의 이동은 황해북부의 복잡한 해안선과 얕은 해저지형에 의한 에너지손실에 기인한다. 수치모델결과에 나타난 이동된 역풍류의 길은 여름철 황해저층냉수 남하의 길과 잘 일치한다.

• 한국위성정보통신학회논문지
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• 제9권1호
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• pp.107-114
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• 2014

2.1GHz 위성 주파수 대역은 상향 1980-2010 MHz, 하향 2170-2200MHz의 주파수 대역으로 위성 IMT를 포함한 위성이동통신 뿐만아니라 이동통신용으로 사용 가능하도록 규정되어 있으며, 지상 IMT 대역과 인접한 넓은 대역폭을 이용하여 광대역 서비스를 제공할 수 있어, 최근 국제적으로 위성/지상 겸용으로 활용하기 위한 연구들이 진행되고 있다. 위성/지상 겸용망의 효율적인 주파수 활용으로 광대역 멀티미디어 서비스 제공과 공공 안전 및 재난 구호 서비스 제공할 수 있다. 위성과 지상이 동일한 주파수 대역을 활용하여 상호간 서로 재사용하기 위해서는 위성 사용자와 지상 사용자 간의 간섭 조정이 필요하다. 이러한 망간 간섭은 위성/지상 겸용망의 설계에 있어서 가장 중요한 요소라고 할 수 있다. 본 논문에서는 위성통신시스템과 지상통신시스템이 주파수 자원을 공유하여 활용하는 위성/지상 겸용망 시스템에서 위성 업링크에서 지상 단말들로부터 받는 업링크 간섭에 대해 분석하고 위성/지상 겸용망의 업링크 간섭을 완화하기 위한 방안을 살펴본다.