• Proceedings of the Optical Society of Korea Conference
• /
• 2009.02a
• /
• pp.519-520
• /
• 2009

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 1994.10a
• /
• pp.317-322
• /
• 1994

본 연구에서는 원통면 음향 홀로그래피 방법에 대한 세밀한 고찰을 통하여 이 방법을 이용한 음장예측의 실제 적용에 도움을 주고자 한다. 먼저 원통면 음향 홀로그래피 방법의 기본적인 이론에 대한 고찰을 하였고, 원통면 음향 홀로그래피 방법의 실제 적용시 나타나는 창문함수의 영향(window effect)이나 공간상의 엘리어싱(spatial aliasing), 둘러싸기 오차(wraparound error)와 같은 오차에 대하여 모의 실험을 통하여 살펴보았다. 이러한 오차해석을 통하여 가능한 한 오차를 줄이고 신뢰할 수 있는 예측결과를 얻을 수 있는 측정조건을 제시하였으며, 오차를 감소시킬수 있는 Tukey 창문함수의 사용과 제로패딩(zero padding) 방법을 제시하였다. 이러한 기본적인 이해를 바탕으로 원통형 구조물의 방사음장을 예측하는 실험을 하였다.

• Korean Journal of Veterinary Service
• /
• v.39 no.4
• /
• pp.247-251
• /
• 2016

Salmonella is one of the most common bacteria that causes heavy losses in swine industry and major causative pathogen of food poisoning in public health. Various methods for the identification of Salmonella such as Gram staining, agglutination test, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) have been used. Several studies have demonstrated that Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI TOF) Mass Spectrometry (MS) identification is an efficient and inexpensive method for the rapid and routine identification of isolated bacteria. In this study, MALDI TOF MS could provide rapid, accurate identification of Salmonella spp. from swine compared with end point PCR and real time PCR.

• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.749-754
• /
• 2000

PCR primers were designed based on consensus sequences of dTDP-D-glucose 4,6-dehydratase, one of the enzymes involved in the biosynthesis of deoxysugar. The PCR product (360 bp) was obtained from Thermus caldophilus GK24. Colony hybridization was carried out to the cosmid library constructed from T. caldophilus GK24 genomic DNA by the PCR product DNA fragment. We isolated a cosmid clone (pSMTC-1) that was subcloned to call pKCB series plasmid (BamHI fragments), partially sequenced and analyzed. pKCB80 (4.2 kb-BamHI DNA fragment) of them showed ORFs that was orfA, orfB, orfC and orfD. The orfABCD gene cluster is the deosysugar biosynthetic gene ; orfA (glucose-1-phosphate thymidylytransferase), orfB (dTDP-D-glucose 4,6-dehydratase), orfC (dTDP-4-keto-L-rhamnose reductase) and orfD (dTDP-4-keto-6-deoxy-D-glucose 3,5-epimerase). The gene cluster that was related in biosynthesis of dTDP-L-rhamnose was also identified by computer analysis, and we proposed that the biosynthetic pathway of deoxysugar analyzed from DNA sequencing of pKCB80 is from D-glucose-1-phosphate, dTDP-D-glucose, dTDP-4-keto-6-deoxy-D-glucose via dTDP-4-keto-L-rhamnose to dTDP-L-rhamnose.