• Journal of Food Hygiene and Safety
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• v.38 no.6
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• pp.420-429
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• 2023

GC-MS/MS using liquid-liquid extraction (LLE) and C18 cartridges was used to identify and quantify levels of chlorpyrifos, chlorpyrifos-methyl, cypermethrin, deltamethrin and permethrin in bulk raw milk. A calibration curve spanning 10 ng/mL to 200 ng/mL was obtained with a satisfactory correlation coefficient of 0.99. The limits of detection (LOD) and limits of quantitation (LOQ) for chlorpyrifos, chlorpyrifos-methyl, cypermethrin, deltamethrin, and permethrin in the matrix ranged from 0.06 to 1.81 ng/mL and 0.19 to 6.04 ng/mL, respectively. The recoveries of 5 pesticides from spiked samples at 37.5-125 ng/mL ranged from 86.1 to 102.1%. The measurement of uncertainty of the GC-MS/MS method for these five pesticides was developed based on the analytical process and quantification. An analysis method that is easier and faster than the method specified in the Korean food standards codes for analyzing these five pesticides in raw material milk was developed. Moreover, the analytical method for chlorpyrifos, chlorpyrifos-methyl, cypermethrin, deltamethrin, and permethrin in bulk raw milk by GC-MS/MS was established.

• The Korean Journal of Nuclear Medicine
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• v.37 no.6
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• pp.382-392
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• 2003

Purpose: Uptakes of Tc-99m MIBI (MIBI) and Tc-99m tetrofosmin (tetrofosmin) in human non-small cell lung cancer A549, multidrug-resistance associated protein (MRP) expressing cell, were investigated in vitro and in vivo. Materials and Methods: Western blot analysis and immunohistochemistry were used for detection of MRP in A549 cells with anti-MRPr1 antibody. Cellular uptakes of two tracers were evaluated at $100{\mu}M$ of verapamil (Vrp), $50{\mu}M$ of cyclosporin A (CsA) and $25{\mu}M$ of butoxysulfoximide (BSO) after incubation with MIBI and tetrofosmin for 30 and 50 min at $37^{\circ}C$, using single cell suspensions at $1{\times}10^6cells/ml$. Radioactivities in supernatants and pellets were measured with gamma well counter. A549 cells were inoculated in each flanks of 24 nude mice. Group 1 (Gr1) and Gr3 mice were treated with only MIBI or tetrofosmin, and Gr2 and Gr4 mice were treated with 70mg/kg of CsA i.p. for 1 hour before injection of 370KBq of MIBI or tetrofosmin. Mice were sacrificed at 10, 60 and 240 min. Radioactivities of organs and tumors were expressed as percentage injected dose per gram of tissue (%ID/gm). Results: Western blot analysis of the A549 cells detected expression of MRPr1 (190 kDa) and immunohistochemical staining of tumor tissue for MRPr1 revealed brownish staining in cell membrane but not P-gp. Upon incubating A549 cells for 60 min with MIBI and tetrofosmin, cellular uptake of MIBI was higher than that of tetrofosmin. Coincubation with modulators resulted in an increase in cellular uptakes of MIBI and tetrofosmin. Percentage increase of MIBI was higher than that of tetrofosmin with Vrp by 623% and 427%, CsA by 753% and 629% and BSO by 219% and 140%, respectively. There was no significant difference in tumoral uptakes of MIBI and tetrofosmin between Gr1 and Gr3. Percentage increases in MIBI (114% at 10 min, 257% at 60 min, 396% at 240 min) and tetrofosmin uptake (110% at 10 min, 205% at 60 min, 410% at 240 min) were progressively higher by the time up to 240 min with CsA. Conclusion: These results indicate that MIBI and tetrofosmin are suitable tracers for imaging MRP-mediated drug resistance in A549 tumors. MIBI may be a better tracer than tetrofosmin for evaluating MRP reversal effect of modulators.

• Korean journal of food and cookery science
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• v.17 no.1
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• pp.13-22
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• 2001

This study was to investigate the reason of family restaurant cook part employee's turnover. One hundred and forty seven family restaurant employees in Seoul area were surveyed to obtain the information from Oct. 1 to 10 in 2000. There were sixty males and eighty seven females. The group of twenty years old to twenty nine years old(95,2%) was the largest one by age, and the group of junior college graduated(71.4%) was the largest one by learning. On order, manager was 4.1%, captain was 13.6%, and employee was 82.3%. Except 15.6% employee, almost family restaurant cook part employees' service of duty was under 2 years. The highest scored turnover factor was work system(3.59), and then human relation(3.18), another way(3.11), unbelievable management(3.04). The rest factors effected on turnover not too much. The mean of female's turnover factor score(3.06) is higher then male(3.00), the group of over fifty years 0Id(3.32) had the highest mean score in aged group, on learning, the group of Master degree's mean score(4.24) is highest. The manager's mean score(3.23) was highest in order, and the employees who's service duty was over five years(3.35) had the highest mean score in service duty group.

• Journal of Korean Society of Transportation
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• v.33 no.3
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• pp.315-321
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• 2015

ATMS calculates section travel time using two-way communication system called DSRC(Dedicated Short Range Communications) which collects data of RSE (Road Side Equipment) and Hi-pass OBU (On-board Unit). Travel time estimation in urban area involves uncertainty due to the interrupted flow. This study not only analyzed real-time data but also considered pattern data. Baek-Je-Ro street in Jeon-Ju city was selected as a test site. Existing algorithm was utilized for data filtering and pattern data building. Analysis results repoted that travel time estimation with 20% of real-time data and 80% of pattern data mixture gave minimum average difference of 37.5 seconds compare to the real travel time at the 5% significant level. Results of this study recommend usage of intermixture between real time data and pattern data to minimize error for travel time estimation in urban area.

• Korean Journal of Community Nutrition
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• v.7 no.5
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• pp.639-653
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• 2002

The purpose of this study was to investigate the regional 야fferences in dietary supplement use and related factors among college students participating in nutritional education programs via the internet. The subjects in this study were 797 college students (male: 518, female: 279). A cross-sectional survey was conducted using a self-administered questionnaire about dietary supplement use, demographic characteristics, health-related lifestyles, nutrient intake, and data were collected via the internet and by mail. Dietary supplements were taken by 82.2% of subjects (males: 76.3%, females: 85.3%). There was a significant regional difference in supplement use (p<0.01). The supplements, used most frequent by male students in the Seoul and Incheon areas were lactic acid beverages and gagogi, in that order. Male students in the Kyunggi area used gagogi and lactic acid beverages in that order. Lactic acid beverages and Chinese medicine were used most frequently by male students in the Chungcheong area. Male students in the Kyungsang area used lactic acid beverages and water-soluble vitamins, in that order. Male students in Seoul and Kyungsang areas showed significantly higher percentage of keep on taking supplements compared to other areas (p<0.05). Both male and female students in the Seoul area had more frequent medical examinations, in comparison to students in other areas (p<0.05). Male students in the Kyungsang and Seoul areas took significantly more calcium (p<0.05) and iron (p<0.05), in comparison to students in other areas. Female students id the Kyunsang area took significantly more Vitamin A, phosphorous and iron (p<0.05), while those in the Seoul area took significantly more Vitamin C (p <0.01) and calcium (p<0.05) . Male students in the Chungcheong area took significantly less seaweed, in comparison to students in other areas (p<0.05) . Male students in the Seoul area had the highest amount of animal fat and oil, while those in the Incheon area ate the lowest amount of animal oil and fat (p<0.05). Female students in the Kyunggi area ate the highest amount of fruit, while those in the Incheon area ate the lowest amount of fruit (p< 0.05) , Female students in the Incheon area ate the highest amount of seaweed, while those in the Kyunggi area ate the lowest amount of seaweed (p<0.01). Therefore, it can be concluded that it is necessary to develop dietary supplements to optimize the nutritional status of college students in different areas of Korea.

• Research in Plant Disease
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• v.9 no.2
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• pp.94-98
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• 2003

To produce antibodies against Pseudomonas tolaasii and P. agarici, lyophilized P. tolaasii and P. agarici ($5{\times}10^7$ cfu/ml) and Freund, s adjuvant were immunized into rabbits 4 times. The specificity and sensitivity of the antibodies were evaluated by immunodiffusion test and indirect enzyme-linked immunosorbent assay (id ELISA). The ${\alpha}$-P. tolaasii antibody was very specific only against P. tolaasii, while ${\alpha}$-P. agarici antibody was not specific and showed a high cross reactivity toward P. tolaasii with detection limit concentration of $2{\times}10^3$ cfu/ml. However, the cross reactivities of ${\alpha}$-P. agarici antibody toward the related species including P. reactans were very low. Our results showed that ${\alpha}$-P. tolaasii and ${\alpha}$-P. agarici antibodies against P. tolaasii and P. agarici, respectively, might be useful for rapid and simple detection of the causal agents of bacterial brown and yellow blotches in cultivated oyster mushrooms.

• The Journal of Korean Academy of Prosthodontics
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• v.31 no.1
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• pp.129-150
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• 1993

Since the restoration or masticatory function is the most important aim of implants, it should be substituted for the role of natural teeth and deliver the stress to the bone under the continous load during function. In natural teeth, stress distribution can be obtained through enamel, dentin and cementum and the elasticity of the periodontal ligament play a role of buffering action. In contrast, implant prosthesis has a very unique characteristics that it delvers the load directly to bone through the implant and superstructure. This fact arise the needs to evaluate the stress distribution of the implant in the mechnical aspects, which has a similar role of natural teeth but different pathway of stress. With 3 kinds of implant in prevalent use, 2 types of experimental PEA implant models were made, axisymmetric and 2-dimensional type. In axisymmetric model, the stiffness of the part including the prosthesis and implant which extrude out of bony surface could be calculated with displacement of the superstructure un er 100N vertical load and then damping effects could be determined through this stiffness. In axisymmetric FEA model, load to the bone could be deduced by evaluation the stress distribution of the designed surface under the 100N vertical force and in 2-dimensional model, 100N eccentric vertical load and 20N horizontal loda. The result are as follows. 1. In every implant, stress to the bone tends to be concenturated on the cortical bone. 2. Though the stress of the cancellous bone is larger at the apex of implants, it is less compared with cortical bone. 3. Under 20N horizontal load, stress of the left and right sides of implant shows a symmetrical pattern. But under 100N eccentric vertical load, loaded side shows much larger stress value. 4. In the 1mm interface, stress distribution among implants tend to have a similar pattern. But under 20N horizontal load apposite side of being loaded shows less stress in IMZ. 5. In the case of screw type implant, stress tends to vary along with screw shape. 6. According to the result determined with microstrain, cancellous bone id generally under the condition of overload, while cortical bone is usually within the limitation of physiologic load. 7. In the Branemark implant, maximum stress to the cortical bone is larger than any other implant except for the condition of 20N horizontal force and 0.05mm interface. 8. Damping effects of implants is maximum in IMZ.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.5 no.2
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• pp.89-100
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• 2019

In our previous studies, we developed a ¹⁸F-labeled TSPO-binding ligand, named [¹⁸F]CB251, which has been proved to be a promising TSPO-binding PET radiotracer for the detection and monitoring of TSPO expression in pathological diseases. (Ki = 0.27 nM for TSPO, 1.96% ID/g of tumor uptake at 1h post-injection) Based on these results, we utilized 6,8-dichloro-2-phenylimidazo[1,2-a]pyridineacetamide analogs, CB185 (1) as a targeting moiety for the selective delivery of probes and anticancer molecules to TSPO-overexpressed tissues. In this study, we designed CB185 derivatives contains different PEG chains (n = 1, 3 and 5) and fluorescence dye (Cy5) to identify the necessary space between a TSPO-binding ligand and an anticancer agent. Three CB185 derivatives (11a-c) which contains Cy5 and PEG chain, were synthesized and the effect of PEG additive on their TSPO-binding affinities were evaluated using in vitro assays. The binding affinity for compounds 11a-c was lower than that of PK11195 (Ki = 3.2 nM), but still characterized by nanomolar binding affinity for TSPO (Ki = 46.5 nM for 11a, 51.0 nM for 11b, and 388.5 nM for 11c). These results showed that the conjugates are characterized by a moderate binding affinity toward TSPO except for compound 11c, which PEG chain consist of five PEG monomers. Our finding might add useful information to decide the appropriate PET chain length for developing new TSPO-targeting drug carriers.

• The Journal of Society for e-Business Studies
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• v.16 no.3
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• pp.1-22
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• 2011

The purpose of this study is to verify the relationship between expectancy confirmation and continuance usage intention in biometric technology. We extend the continuance usage intention model, TAM and expectation confirmation theory by adding perceived privacy, perceived security and trust. Results was analyzed by using structural equations model. The results show that satisfaction and perceived usefulness have positive effect on continuance usage intention in the bright scenario. Perceived privacy and perceived security are positive factors on perceived usefulness, and perceived privacy is positive effect on perceived security. On the other hand, the respondents who are exposed to the dark scenario have negative effects on the perceived privacy, perceived security and trust. And finally, trust has no significant effect on the perceived usefulness.

• Journal of Broadcast Engineering
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• v.14 no.4
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• pp.518-536
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• 2009

To verify the performance of a Distributed Translator Network(DTxR) system in the real world conditions, 5 legacy DTV broadcasting repeater sites were implemented with 5 DTxR systems and field-tested by the DTV field test truck. The 4 DTV broadcasting repeater sites are selected in congested areas where their service areas are overlapped and the 5th site is deviated from the other sites to examine the effect of long-delayed multipath signals. First of all, we checked the receiving signal of the main station that used as a transmitting signal in 5 DTxR systems on the pre-selected 60 test points and tested every case of a DTxR system's on & off except 1 repeater site due to the already built-in DTV repeater system. The test items are received signal electric field strength, noise margin, ease of reception and subjective evaluation of the picture quality for received signals. We used 3rd, 5th, and 6th generation DTV receivers to examine the differences of the receivability by each generation of DTV receivers. Reviewing the test results, we conclude that the DTxR system can be adopted in the current DTV Repeater sites and it could improve the quality and receivability of the main signals by extending the service areas and enhancing the signal levels in the shadow areas without using the extra broadcasting channels.