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New Species of the Genus Metschnikowia Isolated from Flowers in Indonesia, Metschnikowia cibodasensis sp. nov.

  • Sjamsuridzal, Wellyzar;Oetari, Ariyanti;Nakashima, Chiharu;Kanti, Atit;Saraswati, Rasti;Widyastuti, Yantyati;Ando, Katsuhiko
    • Journal of Microbiology and Biotechnology
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    • v.23 no.7
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    • pp.905-912
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    • 2013
  • A novel species, Metschnikowia cibodasensis, is proposed to accommodate eight strains (ID03-$0093^T$, ID03-0094, ID03-0095, ID03-0096, ID03-0097, ID03-0098, ID03-0099, and ID03-0109) isolated from flowers of Saurauia pendula, Berberis nepalensis, and Brunfelsia americana in Cibodas Botanical Garden, West Java, Indonesia. The type strain of M. cibodasensis is ID03-$0093^T$ (= NBRC $101693^T$ =UICC $Y-335^T$ = BTCC-$Y25^T$). The common features of M. cibodasensis are a spherical to ellipsoidopedunculate shaped ascus, which contains one or two needle-shaped ascospores, and lyse at maturity. Asci generally develop directly from vegetative cells but sometimes from chlamydospores. The neighbor-joining tree based on the D1/D2 domain of nuclear large subunit (nLSU) ribosomal DNA sequences strongly supports that M. cibodasensis (eight strains) and its closest teleomorphic species, M. reukaufii, are different species by a 100% bootstrap value. The type strain of M. cibodasensis, ID03-$0093^T$, differed from M. reukaufii NBRC $1679^T$ by six nt (five substitutions and one deletion) in their D1/D2 region of nLSU rDNA, and by 18 nt (five deletions, four insertions, and nine substitutions) in their internal transcribed spacer regions of rDNA, respectively. Four strains representative of M. cibodasensis (ID03-$0093^T$, ID03-0095, ID03-0096, and ID03-0099) showed a mol% G+C content of $44.05{\pm}0.25%$, whereas that of M. reukaufii NBRC $1679^T$ was 41.3%. The low value of DNA-DNA homology (5-16%) in four strains of M. cibodasensis and M. reukaufii NBRC $1679^T$ strongly supported that these strains represent a distinct species.

IP Camera Authentication and Key Exchange Protocol Using ID-Based Signature Scheme (ID 기반 서명 기법을 이용한 IP 카메라 인증 및 키 교환 프로토콜)

  • Park, Jin Young;Song, Chi-ho;Kim, Suk-young;Park, Ju-hyun;Park, Jong Hwan
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.28 no.4
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    • pp.789-801
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    • 2018
  • Currently widely used IP cameras provide the ability to control IP cameras remotely via mobile devices. To do so, the IP camera software is installed on the website specified by the camera manufacturer, and authentication is performed through the password between the IP camera and the mobile device. However, many products currently used do not provide a secure channel between the IP camera and the mobile device, so that all IDs and passwords transmitted between the two parties are exposed. To solve these problems, we propose an authentication and key exchange protocol using ID-based signature scheme. The proposed protocol is characterized in that (1) mutual authentication is performed using ID and password built in IP camera together with ID-based signature, (2) ID and password capable of specifying IP camera are not exposed, (3) provide forward-secrecy using Diffie-Hellman key exchange, and (4) provide security against external attacks as well as an honest-but-curious manufacturer with the master secret key of the ID-based signature.

Pharmacokinetic Properties and Antiinflammatory Activity of Ketoprofen Lotion (케토프로펜 로오숀의 약물동력학적 특성과 항염증작용)

  • 단현광;배준호;박은석;지상철
    • Biomolecules & Therapeutics
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    • v.6 no.1
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    • pp.45-49
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    • 1998
  • The pharmacokinetic properties and antiinflammatory activity of 3% ketoprofen lotion (ID-lotion), formulated with poloxamer 407, were evaluated using rats. For the pharmacokinetic study, the lotion, at the dose of 4.5 mg/kg, was applied on the dorsal skin of rats and the drug concentration in plasma was determined using an HPLC method. As references, ketoprofen suspended in saline was administered orally, and E-lotion, which is a 3% ketoprofen lotion in the Japanese market was applied transdermally. Following the transdermal application of ID-lotion and E-lotion, $C_{max}$ were 316 $\pm$22.3 ng/ml and 163 $\pm$ 12.2 ng/ml, respectively, at the same Tma of 2 hours postdose, while $C_{max}$ and $T_{max}$ after oral administration of the drug were 1,030$\pm$89.1 ng/ml and 0.25 hours, respectively. Relative bioavailabilities of ID-lotion and I-lotion were 69.3% and 34.2%, respectively. The antiinflammatory activity of the two 3% ketoprofen lotions was evaluated with carrageeneninduced edema method after 50 mg of the lotions was applied on the paw of rats. ID-lotion showed 67.6% inhibition of the edema formation, while I-lotion showed 34.\\\\`r%. The calculated ED5o after transdermal application of ID-lotion was 2.5 mg/kg, while that after oral administration was 7.0 mg/kg. Based on these results, the relative equiponderal availability of ID-lotion was 296% compared to the oral administration of ketoprofen.n.n.n.

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Accurate Quantification of Saccharin Using Isotope Dilution Liquid Chromatography Mass Spectrometry (ID-LC/MS)

  • Lee, Yun-Jung;Kim, Byung-Joo;Kim, Jeong-Kwon;Ahn, Seong-Hee
    • Mass Spectrometry Letters
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    • v.2 no.2
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    • pp.37-40
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    • 2011
  • Saccharin is a commonly used artificial sweetener in foodstuffs. However, for its carcinogenic dispute, it has been regulated by government bodies. In this study, isotope dilution mass spectrometry (ID-MS) was introduced for the accurate quantification of saccharin. To employ ID-LC/MS, we obtained its isotope analogue, $^{13}C_1$-sodium saccharin, by customized synthesis. Samples were spiked with $^{13}C_1$-sodium saccharin and analyzed with LC/MS in negative mode. Chromatographic conditions were optimized for the adequate chromatographic retention and separation of saccharin with a $C_{18}$ column. MS was operated with electrospray ionization by the selected ion monitoring (SIM) mode of $[M-H]^-$ for saccharin (m/z 182) and $[M-Na]^-$ for its isotope analogue (m/z 183). To validate the ID-LC/MS method for accurate measurement, we prepared a batch of a candidate material by sortifying quasi-tea-drinks with saccharin and analyzed samples gravimetrically fortified in various levels of concentration. The repeatability and reproducibility of this method was tested by analyzing the reference material. Result show that ID-LC/MS is a reliable method for the quantitative analysis of saccharin.

Low-cost Authentication Protocol Using Pre-synchronized Search Information in RFID System (검색 정보 사전 동기화를 이용한 저비용 RFID 인증 방식)

  • Ha, Jae-Cheol;Park, Jea-Hoon;Ha, Jung-Hoon;Kim, Hwan-Koo;Moon, Sang-Jae
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.18 no.1
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    • pp.77-87
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    • 2008
  • Recently, many hash-based authentication protocols were presented to guarantee mutual authentication between tag and DB in RFID system. To be suitable for distributed DB environment, one generally uses fixed constant value as a tag ID. However, some existing protocols have security flaws or heavy computational loads in DB in order to search a tag ID. We propose a secure authentication protocol which is suitable for distributed DB environment by using unchangeable tag ID. The storage method of pre-synchronized information in DB at previous session is core idea of our proposal which gives low-cost ID search of DB at next session. In normal synchronization state, our protocol only requires 3 hash operations in tag and DB respectively.

Diagnostic approach for genetic causes of intellectual disability

  • Yim, Shin-Young
    • Journal of Genetic Medicine
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    • v.12 no.1
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    • pp.6-11
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    • 2015
  • Intellectual disability (ID) is the most common disability among people under the age of 20 years. In the absence of obvious non-genetic causes of ID, the majority of cases of severe ID are thought to have a genetic cause. The advent of technologies such as array comparative genomic hybridization, single nucleotide polymorphism genotyping arrays, and massively parallel sequencing has shown that de novo copy number variations and single nucleotide variations affecting coding regions are major causes of severe ID. This article reviews the genetic causes of ID along with diagnostic approaches for this disability.

Improved ID-based Authenticated Group Key Agreement Secure Against Impersonation Attack by Insider (내부자에 의한 위장 공격을 방지하는 개선된 ID 기반 그룹 인증 및 키 합의 프로토콜)

  • Park, Hye-Won;Asano, Tomoyuki;Kim, Kwang-Jo
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.19 no.5
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    • pp.25-34
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    • 2009
  • Many conference systems over the Internet require authenticated group key agreement (AGKA) for secure and reliable communication. After Shamir [1] proposed the ID-based cryptosystem in 1984, ID-based AGKA protocols have been actively studied because of the simple public key management. In 2006, Zhou et al. [12] proposed two-round ID-based AGKA protocol which is very efficient in communication and computation complexity. However, their protocol does not provide user identification and suffers from the impersonation attack by malicious participants. In this paper, we propose improved ID-based AGKA protocol to prevent impersonation attack from Zhou et al.'s protocol. In our protocol, the malicious insider cannot impersonate another participants even if he knows the ephemeral group secret value. Moreover, our protocol reduces the computation cost from Zhou et al.'s protocol.

Implementation of UHF Multi-band Multi-protocol u-ID Mobile Reader System (UHF 대역 멀티밴드 멀티프로토콜 ubiquitous-ID 휴대형 리더기 시스템 구현)

  • Ko, Dae-Soo;Kim, Young-Kil
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.11 no.4
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    • pp.707-713
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    • 2007
  • This paper implements a RFID multi-band multi-protocol reader platform, possible to select one of the UHF bands used in particular for distribution system in RFID, that is, from 860MHz to 960MHz, through programmable configuration. It also enables implemented platform in this paper to recognize many kinds of TAG protocol, such as EPC Class 1 GEN 1, Class 1 Gen2, ISO 18000-6A, B and C.

Efficient Beam-Training Technique for Millimeter-Wave Cellular Communications

  • Ku, Bon Woo;Han, Dae Gen;Cho, Yong Soo
    • ETRI Journal
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    • v.38 no.1
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    • pp.81-89
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    • 2016
  • In this paper, a beam ID preamble (BIDP) technique, where a beam ID is transmitted in the physical layer, is proposed for efficient beam training in millimeter-wave cellular communication systems. To facilitate beam ID detection in a multicell environment with multiple beams, a BIDP is designed such that a beam ID is mapped onto a Zadoff-Chu sequence in association with its cell ID. By analyzing the correlation property of the BIDP, it is shown that multiple beams can be transmitted simultaneously with the proposed technique with minimal interbeam interference in a multicell environment, where beams have different time delays due to propagation delay or multipath channel delay. Through simulation with a spatial channel model, it is shown that the best beam pairs can be found with a significantly reduced processing time of beam training in the proposed technique.

ID-based User Authentication Protocol using Smartcard (스마트카드를 이용한 ID기반의 사용자 인증 프로토콜)

  • Lee, Won-Jin;Kim, Eun-Ju;Jeon, Il-Soo
    • Proceedings of the Korean Information Science Society Conference
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    • 2005.07a
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    • pp.166-168
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    • 2005
  • 최근 김등[1]은 스마트카드와 패스워드 그리고 지문 정보를 이용한 ID 기반의 사용자 인증 프로토콜을 제시하였다. 그러나 Scott[2]은 그 프로토콜이 보안에 취약함을 보였다. 본 논문에서는 Scott이 제안한 공격에 안전할 뿐만 아니라 다양한 공격에 안전한 패스워드와 스마트카드를 이용한 ID기반의 사용자 인증 프로토콜을 제안한다.

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