• 한국결정성장학회지
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• 제5권3호
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• pp.197-208
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• 1995

과냉각도가 0.35 K보다 작은 영역에서 ice dendrite의 결정 성장 특성 및 morphology는 과냉각도 뿐만 아니라 자연대류의 함수임을 관찰하였다. 즉, 결정 성장에 대한 자연대류의 영향은 결정 성장 자체에 의한 moving boundary 효과보다 우세함을 알 수 있었다. 본 실험 범위에서 ice dendrite의 성장 속도 및 tip size(edge 및 basal plane)를 과냉각도의 함수로 정량화하였다.

• 한국식품영양과학회지
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• 제21권2호
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• pp.213-218
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• 1992

식품의 동결중에 생성되는 빙결정의 크기에 미치는 동결조건의 영향을 조사하고 그 관계를 정량적으로 평가함과 아울러 동결조건과 빙결정 분산구조와의 관계를 이론해석에 의해 조사한 결과, 1. 동결이동속도 u는 생성빙결정의 평균경 d$_{p}$와 u .d$_{p}$/D~constant의 관계에 있음을 간단한 차원해석에 의해 예측이 가능하였다. 2. 2% 한천 겔, 5%한천 겔, 20% 젤라틴 겔, 돈육의 순서로 동결점에 도달하는 시간이 빨라지며 동결시간 및 최대빙결품 생성대의 통과시간은 냉매온도와 선형적인 관계를 나타내었다. 3. 대두 단백질 겔중에 생성되는 빙결정의 평균경 d$_{p}$와 동결계면 이동속도 u 는 반비례 관계를 나타내었으며, 이로부터 구해진 u .d$_{p}$의 값은 3.4$\times$$10^{-6}$ $\textrm{cm}^2$/sec로 예측 이론식이 유효한 것으로 사료되었다. 4. 동결보존중의 대두 단백질 겔 내부에 생성된 빙결정의 성장에 미치는 보존온도의 영향을 조사한 결과, 양자가 빙결정의 성장에 미치는 영향은 거의 없는 것으로 나타났다. 5. 동결조건과 동결계면구조와의 사이에 Moving velocity of freezing front= (Mass transfer rate of water at freezing front)$\times$ (Surface area of freezing front) 의 관계를 나타내었다.

• 한국축산식품학회지
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• 제32권5호
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• pp.627-634
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• 2012

This study was carried out to investigate the physical and thermal properties of ${\kappa}$-carrageenan (${\kappa}$-car) gel added whey protein isolate (WPI) as a cryoprotectant. The concentration of ${\kappa}$-carrageenan was fixed at 0.2 wt%. The mean ice crystal size of the WPI/${\kappa}$-car was decreased according to increasing whey protein isolate concentration. The temperature of gel-sol (Tg-s) and sol-gel (Ts-g) transition of WPI/${\kappa}$-car maxtrix was represented in the order of 3.0, 0.2, 5.0 and 1.0 wt%. In addition, the transition temperature of gel-sol of WPI in sucrose solution were showed in order of 1.0, 5.0, 0.2 and 3.0 wt% depending on whey protein isolate concentration. The shape of ice crystal was divided largely into two types, round and rectangular form. 1.0 wt% WPI/${\kappa}$-car matrix at pH 7 and 9 showed minute and rectangular formation of ice crystals and whey protein isolate in sucrose solution at a concentration of 1.0 wt% WPI/${\kappa}$-car matrix at pH 3 and 5 showed relatively large size and round ice crystals. The ice recrystallization characteristics and cryprotective effect of ${\kappa}$-carrageenan changed through the addition of different concentrations of whey protein isolate. It seems that the conformational changes induced interactions between whey protein isolate and ${\kappa}$-carrageenan affected ice recrystallization.

• 한국식품과학회지
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• 제27권5호
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• pp.783-788
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• 1995

본 연구는 동결속도 및 동결저장온도가 취반된 쌀의 이화학적 특성에 미치는 영향을 살펴보고자, 단열재를 이용하여 최대 빙결정 형성신간이 3시간, 5시간, 7시간 그리고 12시간인 동결속도로 24시간 동안 동결하였고, 동결된 시료는 각각 $-20^{\circ}C$와 $-70^{\circ}C$의 냉동고에서 3개월간 저장하여 동결저장동안에 각 시료의 노화도 그리고 조직감의 변화를 조사하였다. 동결된 쌀밥의 미세구조 및 표면구조는 빙결정을 동결치환에 의해 제거한후 scanning electron microscopy(SEM)으로 관찰하였다. 취반된 쌀의 노화도는 동결속도, 동결저장온도, 저장기간에 따라 큰 영향을 받아 최대 빙결정 형성시간이 3시간에서 12시간으로 길어짐에 따라 노화도는 14.85%에서 40.00%로 증가하였고, 동결된 쌀밥의 동결저장 중 노화도는 $-20^{\circ}C$에서 저장한 것이 $-70^{\circ}C$의 경우보다 노화된 정도로 크고, 빠른 진행을 보였다. 동결된 시료를 해동한 후의 경도는 동결시키지 않은 대조구와 비교할 때 크게 증가하였으며, 또한 동결속도가 늦어질수록 더 큰 증가를 보였다. 하지만, 3개월간 저장한 후에는 경도가 다시 감소하였다. 한편, 부착성은 대조구와 비교하여 24시간 동안 동결된 쌀밥에서는 감소하였으며 3개월간 저장한 후에는 다시 증가하는 경향을 보였다. 동결된 취반된 쌀의 빙결정의 크기는 동결속도가 늦어짐에 따라 커졌고 그 수는 상대적으로 적었다. 또한 3개월간 저장한 후에는 얼음의 재결정화에 의해 시료의 빙결정은 더욱 커져 취반된 쌀의 구조가 많이 파손되었음을 확인할 수 있었다. 결론적으로 최대빙결정형성시간을 단축하여 취반된 쌀을 동결시키는 것과 낮은 온도에서 저장하는 것이 빙결정의 형성과 성장에 따른 전분의 노화와 조직감의 변화를 억제할 수 있다고 판단되었다.

• 한국축산식품학회지
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• 제34권1호
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• pp.33-39
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• 2014

This study investigates the effects of the gelatin concentration (10-40%, w/v), freezing temperatures (from $-20^{\circ}C$ to $-50^{\circ}C$) and freezing methods on the structural and physical properties of gelatin matrices. To freeze gelatin, the pressure-shift freezing (PSF) is being applied at 0.1 (under atmospheric control), 50 and 100 MPa, respectively. The freezing point of gelatin solutions decrease with increasing gelatin concentrations, from $-0.2^{\circ}C$ (10% gelatin) to $-6.7^{\circ}C$ (40% gelatin), while the extent of supercooling did not show any specific trends. The rheological properties of the gelatin indicate that both the storage (G') and loss (G") moduli were steady in the strain amplitude range of 0.1-10%. To characterize gelatin matrices formed by the various freezing methods, the ice crystal sizes which were being determined by the scanning electron microscopy (SEM) are affected by the gelatin concentrations. The ice crystal sizes are affected by gelatin concentrations and freezing temperature, while the size distributions of ice crystals depend on the freezing methods. Smaller ice crystals are being formed with PSF rather than under the atmospheric control where the freezing temperature is above $-40^{\circ}C$. Thus, the results of this study indicate that the PSF processing at a very low freezing temperature ($-50^{\circ}C$) offers a potential advantage over commercial atmospheric freezing points for the formation of small ice crystals.

• Journal of Biosystems Engineering
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• 제39권4호
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• pp.330-335
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• 2014

Purpose: This study was conducted for enhancing the performance of a conventional quick freezer by introducing the supercooling state, using a low-temperature coolant. Methods: In the present investigation, the supercooling process was executed prior to quick freezing for reducing the time by which the temperature passes the zone of maximum ice crystal formation. Every food has different nucleation points and hence, we used silicone oil as the coolant for supercooling for easy modification of temperature. Additionally, for quick freezing, we used liquid nitrogen spray. Results: Using the heat exchanger-type precooler with silicone oil, the temperature of the chamber was easily changed for enabling supercooling. Particularly, the results of the freezing test with garlic indicated that this system improved the hardness of garlic after it was thawed, compared to the conventional freezing method. Conclusions: Before quick freezing, if the food item is subjected to the supercooling state, the time from nucleation to the temperature reaching the frozen state ($-5^{\circ}C$, which is the maximum ice crystal formation zone) will be shorter than that incurred using quick freezing alone. The combination of the heat exchanger-type supercooler and liquid nitrogen sprayer is expected to serve as a promising technology for improving the physicochemical qualities of frozen foods.

• Integrative Medicine Research
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• 제6권1호
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• pp.12-18
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• 2017

Cryopreservation is a process that preserves organelles, cells, tissues, or any other biological constructs by cooling the samples to very low temperatures. The responses of living cells to ice formation are of theoretical interest and practical relevance. Stem cells and other viable tissues, which have great potential for use in basic research as well as for many medical applications, cannot be stored with simple cooling or freezing for a long time because ice crystal formation, osmotic shock, and membrane damage during freezing and thawing will cause cell death. The successful cryopreservation of cells and tissues has been gradually increasing in recent years, with the use of cryoprotective agents and temperature control equipment. Continuous understanding of the physical and chemical properties that occur in the freezing and thawing cycle will be necessary for the successful cryopreservation of cells or tissues and their clinical applications. In this review, we briefly address representative cryopreservation processes, such as slow freezing and vitrification, and the available cryoprotective agents. In addition, some adverse effects of cryopreservation are mentioned.

• Fisheries and Aquatic Sciences
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• 제2권1호
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• pp.12-16
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• 1999

Denaturation of actomyosin from the obliquely striated mantle muscle of squids (Todarodes pacificus) was studied by measuring the changes in $Ca^{2+}$-ATPase activity, relative viscosity, and solubility during frozen storage at three different temperature zones of maximum ice crystal formation $(-3^{\circ}C,\;-\;-5^{\circ}C)$, the eutectic point $(-11^{\circ}C)$, and $-20^{\circ}C$. The logarithms of $Ca^{2+}$-ATPase activity, relative viscosity and solubility of the actomyosin solutions (0.6 M KCl) and suspensions (0.05 M KCl) tended to decrease during frozen storage. The denaturation of squid actomyosin at the zone of maximum ice crystal formation significantly differed by only two degree of temperature difference between $-3^{\circ}C$ and $-5^{\circ}C$, and it (0.05 M KCl) at $-3^{\circ}C$ was less than those of other temperature. The denaturation at $-11^{\circ}C$ was more rapid than at $-5^{\circ}C$. The logarithms of $Ca^{2+}$ -ATPase activity, relative viscosity, and solubility were changed slower in the suspensions (0.05 M KCl) than the solutions (0.6 M KCl) at all experimental temperatures.

• The Korean Journal of Pain
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• 제9권1호
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• pp.215-218
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• 1996

Frostbite involves freezing of tissues and usually affects the distal aspects of the extremities or exposed parts of the face. such as the ears, nose, chin, and cheeks. It produces tissue injury by ice crystal formation between the cells, cellular dehydration, and microvascular occulsion. There are four degrees of frostbite. First degree is accompanied by erythema and edema; second degree, by vesiculation, blistering, and eschar formation; third degree, by hemorrhagic blistering and bluish gray discoloration; and fourth degree, by injury to subcutaneous tissue, muscle, tendon, and bone leading to mottled, dry, black, and necrotic changes. We successfully treated 2 patients suffering from frostbite by performing sympathetic ganglion block with pure alcohol. We concluded sympathetic ganglion block is one of the most effective treatments for frostbite.

• 한국산림과학회지
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• 제98권1호
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• pp.115-124
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• 2009

새로운 균주가 육종되었을 때 그 균주가 장기간 고유특성을 유지하기란 쉽지 않으며, 시간이 경과 할수록 균주는 퇴화를 거듭한다. 이에 따라 현재 많이 이용되고 있는 초저온에서의 균주보존 후 균사의 활력과 온도가 생존에 미치는 영향과 표고균주가 동결하는 동안 나타나는 상태변화를 이해하고자 하였다. 저온 처리별 균주보존결과 표고균주의 생존은 $-20^{\circ}C$에서 50일간 보존하면 사멸했으며, $-80^{\circ}C$와 $-196^{\circ}C$에서 보존한 균주들은 온도에 영향을 받지 않고 생존하였다. 저온 처리별 균사생장속도는 $-80^{\circ}C$에서 보존한 균주들의 재생속도가 가장 빨랐다. 균사의 정단부분과 말단부분에 대한 저온 처리별 균주보존 결과 부분별 위치는 균사의 활력과 생존에 영향을 끼치지 못했다. 프로그램이 가능한 동결장치를 이용하여 표고균주의 동결과정에서 나타나는 현상을 조사한 결과 균주별 각기 다른 어는점을 가지고 있었다. 또한 표고의 자실체 발생 온도형과 균주별 어는점과의 관계는 관련이 없었다. 프로그램이 가능한 동결장치를 이용하여 표고균주의 동결과정에서 나타나는 상태변화를 최소화하기 위해 일시적으로 낮은 온도로 동결을 시도하였지만 균주들은 온도에 영향을 받지 않았다.