• Animal cells and systems
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• 제12권4호
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• pp.203-209
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• 2008

Berberine, an isoquinoline alkaloid found in Coptidis Rhizoma(goldenthread) extract, has multiple pharmacological effects such as anti-inflammatory, antimicrobial and anti-ischemic effects. In the present study, we examined the effects of berberine on neuronal survival and differentiation in a hippocampal precursor cell line and in the memory deficient rat model. Berberine increased in a dose dependent manner the survival of hippocampal precursor cells as well as differentiated cells. In addition, berberine promoted neuronal differentiation of hippocampal precursor cells. In the memory deficient rat model induced by stereotaxic injection of ibotenic acid into entorhinal cortex(Ibo model), hippocampal cells were increased about 2.7 fold in the pyramidal layer of CA1 region and about 2 fold in the dentate gyrus by administration of berberine after 2 weeks of ibotenic acid injection. Furthermore, neuronal cells immunoreactive to calbindin were increased in the hippocampus and entorhinal cortex area by administration of berberine. Taken together, these results suggest that berberine has neuroprotective effect in the Ibo model rat brain by promoting the neuronal survival and differentiation.

• The Korean Journal of Physiology and Pharmacology
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• 제23권6호
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• pp.483-491
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• 2019

Cordycepin exerts neuroprotective effects against excitotoxic neuronal death. However, its direct electrophysiological evidence in Alzheimer's disease (AD) remains unclear. This study aimed to explore the electrophysiological mechanisms underlying the protective effect of cordycepin against the excitotoxic neuronal insult in AD using whole-cell patch clamp techniques. ${\beta}$-Amyloid ($A{\beta}$) and ibotenic acid (IBO)-induced injury model in cultured hippocampal neurons was used for the purpose. The results revealed that cordycepin significantly delayed $A{\beta}$ + IBO-induced excessive neuronal membrane depolarization. It increased the onset time/latency, extended the duration, and reduced the slope in both slow and rapid depolarization. Additionally, cordycepin reversed the neuronal hyperactivity in $A{\beta}$ + IBO-induced evoked action potential (AP) firing, including increase in repetitive firing frequency, shortening of evoked AP latency, decrease in the amplitude of fast afterhyperpolarization, and increase in membrane depolarization. Further, the suppressive effect of cordycepin against $A{\beta}$ + IBO-induced excessive neuronal membrane depolarization and neuronal hyperactivity was blocked by DPCPX (8-cyclopentyl-1,3-dipropylxanthine, an adenosine $A_1$ receptor-specific blocker). Collectively, these results revealed the suppressive effect of cordycepin against the $A{\beta}$ + IBO-induced excitotoxic neuronal insult by attenuating excessive neuronal activity and membrane depolarization, and the mechanism through the activation of $A_1R$ is strongly recommended, thus highlighting the therapeutic potential of cordycepin in AD.

• 한국전자파학회논문지
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• 제13권6호
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• pp.538-545
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• 2002

OFDM 통신 시스템에서는 피크전력 대 평균전력비(Peak to Average Power Ratio, PAPR)가 커짐에 따라 전력증폭기에서 비선형 왜곡이 발생되므로 이를 포함한 전송 성능평가가 중요하다. 본 논문에서는 전력증폭기에서 비선형 왜곡이 있는 OFDM 시스템의 성능을 평가하는 새로운 혼합 분석기법을 제안한다. 즉, 제안된 혼합 분석기법은 시뮬레이션을 이용하여 먼저 PAPR의 확률밀도함수와 비선형 잡음의 분산과 평균을 구하고, 이를 이용하여 해석적(analytical) 방법으로 전체 BER을 구하는 방법이다. QPSK 또는 16-QAM 변조방식의 OFDM 시스템에서, 반도체전력증폭기(Solid-State-Power Amplifier, SSPA)의 등가모델을 적용하고, PAPR을 파라미터로 사용하여 IBO 크기에 따른 BER을 분석한다. 제안 방법이 기존의 Monte-Carlo 시뮬레이션에 의한 BER과 거의 동일한 분석 결과를 보이고, 기존 방법보다 BER곡선을 얻는데 필요한 시간이 상당히 감소됨을 보인다