• 제목/요약/키워드: IST

검색결과 396건 처리시간 0.023초

해조류로부터 Arbitrary 및 ITS Primer들을 사용한 직접 PCR 유전자 증폭반응의 한계 (Limits of Direct PCR Amplification from Seaweeds Using Arbitrary and ITS Primers)

  • 김용국;진형주;박선미;진덕희;홍용기
    • 생명과학회지
    • /
    • 제9권1호
    • /
    • pp.15-21
    • /
    • 1999
  • PCR 기법을 응용한 RAPD 방법은 대상 생물의 유전정보를 전혀 모르는 상태에도 arbitrary primer를 이용하여 증폭함으로써 생물 종간의 유전적 표식자를 확인할 수 있는 간편하고 유익한 유전분석 방법이다. 이같은 RAPD 방법을 이용하여 해조류 방사무늬 김, 미역, 구멍갈파래에 대하여 DNA를 추출하지 않고 직접 생 엽체 및 생 사상체, 생 배우체를 PCR template로 사용하여 PCR product를 생산하였다. 그러나 specific primer를 이용한 nulear r DNA의 internal trancribed spacer (ITS) 부위는 생성물을 만들지 못하였다. 간편한 LiCl 방법에 의하여 추출된 DNA를 사용하였을 때는 IST 및 RAPD 모두 PCR product를 생산하였다. 방사무늬 김의 엽체 (haploid)와 사상체 (dip-loid)로 부터의 ITS 생성물은 동일하였으나 RAPD 생성물은 사용한 arbitrary primer에 따라 36-50$\%$의 다른 band가 만들어졌다. 또한 직접 생 조직을 사용하였을때와 추출 DNA를 사용하였을 때도 53-57$\%$의 다른 band가 만들어 줬다. 그러므로 RAPD 방법으로 유전분석 실험에는 동일한 ploidy의 조직을 template로서 사용하는 것이 중요하다.

  • PDF

의사에게 치료상의 과오를 설명할 의무가 있는가? (Besteht die Pflicht des Arztes, den Patienten ${\ddot{u}}$ber einen eigenen Behandlungsfehler zu informieren?)

  • 김민중
    • 의료법학
    • /
    • 제15권2호
    • /
    • pp.165-194
    • /
    • 2014
  • Der Vertrag zwischen dem Arzt und seinem Patienten wird als Auftrag im Sinne des ${\S}680$ KBGB qualifiziert. Dem Arzt erwachsen innerhalb dieses Behandlungsvertrages zahlreiche Pflichten, von denen ein gro${\ss}$er Teil durch Richterrecht geschaffen wurde. Den Arzt treffen z.B. Behandlungspflicht, Informationspflicht ${\ddot{u}}$ber die Behandlung, Aufkl${\ddot{a}}$rungspflicht ${\ddot{u}}$ber einwilligungspflichtige Umst$\ddot{a}$nde, Dokumentationspflicht, Schweigepflicht. Der Arzt ist nach Rechtsprechung und Literatur verpflichtet, den Patienten ${\ddot{u}}$ber s${\ddot{a}}$mtliche f${\ddot{u}}$r die Einwilligung wesentlichen Umst${\ddot{a}}$nde aufzukl${\ddot{a}}$ren, insbesondere ${\ddot{u}}$ber Art, Umfang, Durchf${\ddot{u}}$hrung, zu erwartende Folgen und spezifische Risiken der Ma${\ss}$nahme, die Notwendigkeit, Dringlichkeit und Eignung der Ma${\ss}$nahme zur Diagnose oder zur Therapie und uber die Erfolgsaussichten der Ma${\ss}$nahme im Hinblick auf die Diagnose oder Therapie. Mu${\ss}$ der Arzt den Patienten auf einen eigenen Behandlungsfehler hinweisen, wenn f$\ddot{u}$r ihnen Umst${\ddot{a}}$nde erkennbar sind, die die Annahme eines Behandlungsfehlers begr${\ddot{u}}$nden. Allgemeine Offenbarungspflichr bei ${\ddot{a}}$rztlichen Behandlungsfehlern wird bisher nicht diskutiert. Nach derzietigem Recht besteht keine allgemeine Offenbarungspflicht des Arztes, den Patienten unaufgefordert ${\ddot{u}}$ber einen eigenen Behandlungsfehler hinzuweisen. Aber wie im ${\S}630c$ BGB, sind f${\ddot{u}}$r den Behandelnden Umst${\ddot{a}}$nde erkennbar, die die Annahme eines Behandlungsfehlers begr${\ddot{u}}$nden, hat er den Patienten ${\ddot{u}}$ber diese auf Nachfrage oder zur Abwendung gesundheitlicher Gefahren zu informieren.

  • PDF

Fabrication of Metallic Particle Dispersed Ceramic Based Nanocomposite Powders by the Spray Pyrolysis Process Using Ultrasonic Atomizer and Reduction Process

  • Choa, Y.H.;Kim, B.H.;Jeong, Y.K.;Chae, K.W.;T.Nakayama;T. Kusunose;T.Sekino;K. Niibara
    • 한국분말재료학회지
    • /
    • 제8권3호
    • /
    • pp.151-156
    • /
    • 2001
  • MgO based nanocomposite powder including ferromagnetic iron particle dispersions, which can be available for the magnetic and catalytic applications, was fabricated by the spray pyrolysis process using ultra-sonic atomizer and reduction processes. Liquid source was prepared from iron (Fe)-nitrate, as a source of Fe nano-dispersion, and magnesium (Mg)-nitrate, as a source of MgO materials, with pure water solvent. After the chamber were heated to given temperatures (500~$^800{\circ}C$), the mist of liquid droplets generated by ultrasonic atomizer carried into the chamber by a carrier gas of air, and the ist was decomposed into Fe-oxide and MgO nano-powder. The obtained powders were reduced by hydrogen atmosphere at 600~$^800{\circ}C$. The reduction behavior was investigated by thermal gravity and hygrometry. After reduction, the aggregated sub-micron Fe/MgO powders were obtained, and each aggregated powder composed of nano-sized Fe/MgO materials. By the difference of the chamber temperature, the particle size of Fe and MgO was changed in a few 10 nm levels. Also, the nano-porous Fe-MgO sub-micron powders were obtained. Through this preparation process and the evaluation of phase and microstructure, it was concluded that the Fe/MgO nanocomposite powders with high surface area and the higher coercive force were successfully fabricated.

  • PDF

플라즈마질화에서 발생기 질소와 질화 속도에 관한 연구 (The Effect of Activated Nitrogen Species for Diffusion Rate during a Plasma Nitriding Process)

  • 김상권;김성완
    • 열처리공학회지
    • /
    • 제23권3호
    • /
    • pp.150-155
    • /
    • 2010
  • Generally, plasma nitriding process has composed with a nitriding layer within glow discharge region occurred by energy exchange. The dissociations of nitrogen molecules are very difficult to make neutral atoms or ionic nitrogen species via glow discharge area. However, the captured electrons in which a double-folded screen with same potential cathode can stimulate and come out some single atoms or activated ionic species. It was showed an important thing that is called "hat is a dominant component in this nitriding process?" in plasma nitriding process and it can take an effective species for without compound layer. During a plasma nitriding process, it was able to estimate with analyzing and identification by optical emission spectroscopy (OES) study. And then we can make comparative studies on the nitrogen transfer with plasma nitriding and ATONA process using plasma diagnosis and metallurgical observation. From these observations, we can understand role of active species of nitrogen, like N, $N^+$, ${N_2}^+$, ${N_2}^*$ and $NH_x$-radical, in bulk plasma of each process. And the same time, during DC plasma nitriding and other processes, the species of FeN atom or any ionic nitride species were not detected by OES analyzing.

분포계수차가 매우 작은 물질의 이온교환수지에 의한 분리 (Ionenaustauschertrennung von Substanzen mit geringem Unterschied im Verteilungskoeffizienten)

  • 김동원
    • 대한화학회지
    • /
    • 제24권6호
    • /
    • pp.426-433
    • /
    • 1980
  • 컬럼 단면적에 대한 $V_{max}$$V_{HWB}$ 크기의 의존성이 기대했던 것과 같이 직선적인 함수 관계에 있음을 알 수 있었다. 이 의존성은 일반적으로 가장 굵은 입자 크기인 20∼50 mesh의 이온교환수지를 사용한 지름 3 mm의 컬럼에 의하여 증명되었다. 유속의 영향은 $V_{max}$값에 대해서는 거의 효과가 없었으나, $V_{HWB}$값에 대해서는 강한 영향을 주는 긍정적 항으로서 작용한다는 것을 알 수 있었다. 유속이 빠르면 Bandenverbreitung은 급격히 증가되었다. 이와 같은 사실은 분리곤란한 물질의 분리 문제엔 느린 유속이 유익하다는 것을 보여 준다. 흡착량은 $V_{max}$$V_{HWB}$값에 부가적으로 영향을 주었다. 분리효과가 더 이상 이루어 질 수 없도록 많은 양의 방사성핵 (Co 와 Mn)을 사용했을 때는 $V_{max}$값의 차이는 미소하였다. Co($K_D$=68.2)와 Mn($K_D$=71.8)의 분리를 통하여, 만들어 낸 공식을 검증하였고 또한 그것이 잘 적용될 수 있음을 증명할 수 있었다.

  • PDF

요추관 협착증과 골밀도와의 상관성에 관한 임상례 보고 (Correlation between lumbar spinal stenosis and bone mineral density : a clinical survey of 9 cases)

  • 송주현;강인;임명장;김하늘;이제균;장형석
    • 척추신경추나의학회지
    • /
    • 제1권1호
    • /
    • pp.105-111
    • /
    • 2006
  • Objective : The aim of this study was to evaluate association between lumbar spinal stenosis, one of degenerative diseases, and bone mineral density. Methods : We monitored 9 lumbar spinal stenosis patients in women above 50 years, visiting outpatient clinic of the Jaseng Oriental Medicine Hospital between January 5 2006 and March 31, 2006. They were diagnosed by radiologist ist after taking Magnetic Resonance Imaging(MRI). The cases were Investigated the bone mineral density using Dual Energy X-ray Absorptiomerty(DEXA). and then Picture Archiving Communication System(PACS) were used to assess correlation between lumbar spinal stenosis and bone mineral density. Results : 1. In comparison of the spinal canal area and lumbar spine 2 level bone mineral density, the data showed a significant result 2. The data, between spinal canal area and lumbar spine 1-2 level bone mineral density, indicated a significant result. 3. Also, the result of comparison between spinal canal area and the lowest value of bone mineral density showed significance. Conclusions : It showed that there is a statistically significant correlation between lumbar spinal stenosis and bone mineral density.

  • PDF

화행의 하위 분류 가능성에 대한 고찰 - '이유 말하기'를 중심으로 - (Zur $M\"{o}glichkeit$ der Unterklassifikation von Sprechakttypen. Am Beispiel der $begr\"{u}ndenden$ Sprechhandlungen)

  • 강창우
    • 한국독어학회지:독어학
    • /
    • 제5집
    • /
    • pp.203-221
    • /
    • 2002
  • Die Unterklassifikation von Sprechakttypen stellt einen wichtigen Forschtmgs­gegenstand der Sprechakttheorie dar. Mit den Kriterien, die Searle (1969) vorgeschlagen hat, $k\"{o}nnen\;zwar\;die\;f\"{u}nf$ Haupttypen der Sprechakte voneinander unterschieden werden, aber $f\"{u}r$ deren Unterklassifikation $m\"{u}ssen$ weitere Kriterien herangezogen werden, und zwar solche, mit denen die Untertypen des zu unterklassifizierenden Sprechakttyps deren Handlungscharakter $gem\"{a}{\ss}$ klassifiziert werden $k\"{o}nnen$. Es ist aber nicht einfach festzustellen, was man als Kriterien $f\"{u}r$ die Unterklassifikation eines bestimmten Sprechakttyps nehmen soll. In der vorliegenden Arbeit wird versucht, die $f\"{u}r$ die Unterklassifikation der sogenannten $begr\"{u}ndenden$ Sprechhandlungen (= bSH) wie $ERKL\"{A}REN-WARUM,\;BEGR\"{U}NDEN$, RECHTFERTIGEN und ARGUMENTIEREN angemessenen Kriterien festzustellen und mit diesen die vier Untertypen der bSH zu klassifizieren. Dabei werden zwei Kriterien herangezogen: $n\"{a}mlich$, Gegenstandstyp und Strittigkeit. Je nachdem, urn welchen Gegenstandstyp es bei der bSH geht und ob Strittigkeit vorliegt, wird ein anderer Untertyp der bSH vollzogen. Die Gegenstandstypen der bSH sind wie folgt darzustellen: Gegenstandstypen der bSH Gegenstandstypen im subjektiven Bereich Gegenstandstrypen im objektiven Bereich Einstellungen des Sprechers Handlungen des Sprechers Tatsachen Ereignisse Naturprozesse Handlungen eines Dritten Einstellungen eines Dritten Strittigkeit kann nur in Bezug auf die Gegenstandstypen im subjektiven Bereich entstehen, und zwar in Bezug auf die Richtigkeit der Handlungen und Einstellungen und die Wahrheit der Einstellungen des Sprechers. Mit den beiden Kriterien 'Gegenstandstyp' und 'Strittigkeit' $k\"{o}nnen$ die Untertypen der bSH wie folgt klassifiziert werden:

  • PDF

독일어 제 2분사(Partizip II)의 부가전적인 형용사화에 따른 문법상의 제약 (Untersuchungen zur Grammatikalitat der attributiven Adjektive aus dem Partizip II)

  • 이해정
    • 한국독어학회지:독어학
    • /
    • 제8집
    • /
    • pp.61-87
    • /
    • 2003
  • In diesem Aufsatz versuche ich, die $Grammatikalit\"{a}t$ der attributiven Adjektive zu beurteilen, die aus ihren Basisverben abgeleitet sind. In den bisherigen Untersuchungen der deutschen Syntax sind die $Ausdr\"{u}cke$ wie das verbrannte Papier, die $aufgebl\"{u}hte$ Rose, die verklungene Glocke als grammatisch angenommen worden. All diejenigen Adjektive sind aus den Ereignissverben abgeleitet. Fraglich ist nun, ab man die Attribuierung der Adjektive aus den Fortbewegungs­verben kommen, gehen, laufen auch fur akzeptabel halten soli. Einige Beispiele wie der in Heidelberg angekommene Zug, der aus dem $B\"{u}ro$ gelaufene Mann zeigen, dass die Ableitung der attribuierten djektive auch fur Fortbewegungsverben $m\{o}glich$ sei. Auf der anderen Seite kann man diejenigen Beispiele schwer akzeptieren, wie der zum Bahnhof gegangene Mann, der $\"{u}ber$ den Fluss geschwommene Junge, die aus kommen, gehen, laufen usw. abgeleitet sind. Die entscheidenden Kriterien der $Grammatikalit\"{a}t$ liegen darin, dass die Ableitung der attribuierten Adjektive $haupts\"{a}chlich$ folgende Bedingungen $erf\"{u}llen$ soli: 1. Das an dem Ereignis beteiligte Argument muss eine Theta-Rolle [THEME] haben. 2. Das Argument soli irgendwie qualitativ oder quantitativ $ver\"{a}ndert$ werden. 3. Das Ereignis muss eine $Ver\"{a}nderung$ zeigen, die Anfangszustand, Endzustand und auch eine $\"{U}bergangszeit$ dazwischen beinhaltet. Mit diesen Kriterien habe ich die $Grammatikalit\"{a}t$ der wichtigen Beispiele der Attribuierung entscheiden $k\"{o}nnen$, die in der deutschen Linguistik theoretisch viel diskutiert worden sind.

  • PDF

일 종합병원 간호사의 간호행위에 대한 환자 만족도 조사연구 (A Study on the patient satisfaction for nursing care in a general hospital.)

  • 강익화
    • 간호행정학회지
    • /
    • 제3권2호
    • /
    • pp.137-150
    • /
    • 1997
  • This study was done to evaluate patient satisfaction with nursing care. The study subjucts were 164 patients admitted in a general hospital in Incheon province. The instrument used in this study was developed by Risser (1975) and Lim Hae Kyoung (1980) based on classification of nursing care area and modified by Chow Kwang Nam (1995). The relaiability alpha of this tool was 0.93695. The survey method was applied to collect data and the period of data collection was total 30 days from April Ist to April 30th in 1997. The data was analyzed by statistical method of %, mean, t-test and ANOVA through SAS program. 1. The highest scores of satisfaction were shown in the area of reliance, area of treatment, area of environment and area of education in order. 2. The crossed analysis of patient general characteristics and satisfaction of nursing care showed a signficance in the department of patient's admission and the highest satisfaction score was found in the general surgery ward (p<0.05). 3. General characteristics of patients which influence on the satisfaction showed a significant response according to the department of patient's admission in the treatment area, education area and environment area of nursing.(p < 0.05). Recommendation The followings are deriven from the above study results. 1. Area of nursing education which showed the lowest satisfaction should be empathized by making improvement of nursing care approach because the area in the basis to achieve nursing goal. 2. The development of systematic and objective instrument is necessary for evaluation of nursing quality and the evaluation should be performed in the area of patient satisfaction, hospital facility and hospital environment as well.

  • PDF

단위수산업협동조합의 이사회제도에 대한 수용자태도분석 (Analysis of Accepters' Attitudes toward the Board of Directors System of Fisheries Cooperatives)

  • 정만화;최정윤
    • 수산경영론집
    • /
    • 제36권3호
    • /
    • pp.37-63
    • /
    • 2005
  • According to the Fisheries Cooperative Law, the Fisheries Cooperative has made a substantial contribution to the development of Korea's fisheries industry amid the nation's rapid economic growth and drastic social changes since its establishment in April 1st 1962, It is, however, generally believed that the cooperative has made a poor performance in improving its business constitution. Therefore, the reform of its board of director system is one of the ways to strengthen the constitution. For the purpose of making the board of director system to operate rationally, this dissertation is divided into five chapters. Chapter I provides an overview of this study, such as research background and objectives. Chapter II focuses on the Fisheries Cooperative's board of director: its function, composition, and election. Chapter III analyzes the responses of the Fisheries cooperatives to questionnaires about the board of director system, and subsequently presents the causes and the meaning of the analysis results. Chapter IV deals with the various ways to improve the Fisheries Cooperative's board of director system, including director and cooperative governance. Finally, Chapter V draws a conclusion of this study. The independent function of the Cooperative's board of director and responsible directorship is one of the most important factors which can prevent the Cooperative from insolvency. Unlike the past, not only it has become difficult to keep the Cooperative's business under the government's support, but also the Cooperative itself is now facing a situation where the support from the government cannot be expected. This ist he reason why the Cooperative should manage itself autonomously, workout its organization, and respond to the current situation with a renovativemanagement and business strategy. Therefore, a rational operation of the board of director is urgently needed.

  • PDF