• Journal of Ginseng Research
• /
• v.23 no.2 s.54
• /
• pp.105-114
• /
• 1999

To investigate the effects of korean ginseng on the expression of transferrin receptor (TfR) in the liver cell membrane, we had carried out the experiments of $[^{3}H]thymidine$ uptake, $^{125}I-transferrin$ binding, and TfR mRNA expression in the liver after partial hepatectomy of normal and 3'-methyl-4-dimethylaminoa-zobenzene (3'-Me-DAB) treated rat with or without treatment of korean gingseng. $[^3H]thymidine$ uptake was not changed in the liver of 3'-Me-DAB or ginseng treated rat compared to that of control rat, but increased in that of partial hepatectomy of normal or 3'-Me-DAB treated rat. And this increased $[^{3}H]thymidine$ uptake was lowered slightly by the treatment of ginseng. Transferrin binding sites in the liver plasma membrane of ginseng treated rat with or without partial hepatectomy were similar, but increased in that of 3'-Me-DAB treated rat with or without partial hepatectomy compared to those of each control rat and these increased binding sites were reduced by ginseng treatment. Transferrin binding affinity (l/kd) was not changed by ginseng treatment, but tended to decrease in the liver of 3'-Me-DAB treated rat or in those after partial hepatectomy of all groups and reverse by ginseng treatment in 3'-Me-DAB treated rat. The expression of TfR mRNA was increased in the liver of 3'-Me-DAB treated rat with partial hepatectomy (peak at 24 hours), but lowered by ginseng treatment in this rat. From these results, it is suggested that korean ginseng has no effect on the increased expression of TfR with decreased affinity in the cell membrane of regenerated liver after partial hepatectomy of rat, but could inhibt that of 3'-Me-DAB treated rat through the regulation of DNA synthesis or TfR mRNA in partial.

• Journal of Sasang Constitutional Medicine
• /
• v.12 no.1
• /
• pp.84-100
• /
• 2000

Are the body and th spirit to different things? How individual ability and feeling displays in a human being and what correation between the two lise physiologically? Namely, what determines the external and the internal world? By what physiological functions and circulations, it makes possible to indciate individual's characteristic? These kinds of questions in constitutional medicine is able us lead to the following approach.

• Journal of Veterinary Clinics
• /
• v.31 no.4
• /
• pp.267-271
• /
• 2014

Accurate determination of in vivo oocyte maturation is particularly critical for dog cloning compared to other assisted reproductive technologies because oocytes in metaphase II stage have to be recovered in order to undergo somatic cell nuclear transfer right after its recovery. The aim of present study was to evaluate the reliability and to set a reference range of a chemiluminescence enzyme immunoassay (CLEIA) compared to radioimmunoassay (RIA) method to retrieve in vivo matured oocytes. Serum progesterone concentration during proestrus and estrus was analyzed by RIA and CLEIA to determine ovulation day (Day 0). On Day 3, in vivo oocytes were recovered surgically and evaluated microscopically maturation status after staining nucleus with bisbenzimidazole dye. Mean progesterone concentration by CLEIA ($7.64{\pm}0.06ng/ml$) was significantly higher than by RIA ($6.46{\pm}0.04ng/ml$, P < 0.0001). It was not different between CLEIA ($10.01{\pm}0.34ng/ml$) and RIA values ($7.91{\pm}0.14ng/ml$, P < 0.05) on Day 0, but significantly higher CLEIA level on Day -1 and Day 1 ($6.41{\pm}0.15$ and $14.25{\pm}0.44ng/ml$) was assessed compared to RIA ($4.95{\pm}0.10$ and $11.29{\pm}0.34ng/ml$). However, with both methods, progesterone level was significantly increased from Day -1 to Day 2. To determine oocyte maturation with CLEIA method, a wider and higher reference range has to be considered.

• Korean Journal of Weed Science
• /
• v.12 no.1
• /
• pp.61-69
• /
• 1992

Weed control in mid season of rice fields was systemized earlier in Korea than in Western Europe or China. The most representative method was pull out the weeds by hand followed by 'Seojibeob'(鋤地法 : method of hoeing) after draining. It was practiced 3 to 4 times to do extensively. Also, the purposes of transplanting arid direct seeding in rows were to manage weed problems effectively, which means that the development of cultural techniques is closely related to 'Seojibeob'(鋤地法). From the 17th century, techniques of deep plowing(because of principal : 'Simkyonginu'(深耨易耕 : Deep plowing make easy to hoe) and 'Hwanubeob'(火耨法 : derived from 'Hwakyong soonubeob) for effective weed management of waste lands were newly adopted and spreaded due to the situations ant that time. Thus, the practice of large-scale farming resulted from the adaptation of the techniques and methods described above. In the 18th to 19th century, the cultural methods, not much different from 'Seojibeob', were changed and the number of weeding was decreased. Especially, the techniques of dry seeding of rice were practiced by originating the ideal 'Seojibeob', in which water availability was improved in northern drought areas and also weed problems were easily managed. In addition, intensively large-scale farming showed weed management problems, so that 'Cheonilrok'(千一錄) by Woo Ha-young described the techniques of 'Banjongbeob'(反種法) or 'Hwanubeob' and in the end of the Chosun dynasty extensively large-scale farming was practiced by putting intensive and extensive techniques together. As the results, agricuitural productivity of Korea was increased 5 times by that time.

• Journal of Chest Surgery
• /
• v.30 no.7
• /
• pp.656-662
• /
• 1997

Aortic valvuloplasty has recently gained attention as an attractive alternative procedure for aortic valvular disease. Between March 1995 to August 1996, 14 patients with pure aortic regurgitation(AR) underwent aortic alvuloplasty using leaflet extension with glutaraldehydepreserved autologous pericardium. There were 11 males and 3 females, and the mean age was 34.8 $\pm$ 15.3 years. Preoperative echocardiography and cardiac catheterization revealed that the degree of AR was mean 3.4$\pm$0.65, and more than moderate degree of mitral regurgitation(MR) were detected in 4 patients. In 12 patients, 3 leaflets were extended and in another 2 patients only one deformed leaflet was extended. Concomitant mitral valvuloplasty (MVP) was performed in 4 patients. The competency of the aortic valve after completion of repair was evaluated by the transesophageal echocardiography in operating theater, and there was no aortic and mitral stenosis or regurgitation. In an early postoperative echocardiography, trivial AR was detected in 3 patients and mild MR in 1 patient. The end-systolic and end-diastolic dimensions of the left ventricle were decreased significantly(p<0.05) as compared with those of preoperative values. T ere was no mortality and no significant postoperative complication encountered. Late complication developed in 2 patients during the follow-up period(mean 7.9$\pm$ 5.9 months). One patient underwent AVR on postoperative 7th month due to endocarditis, and the another patient with Behcet's disease underwent Ross operation at postoperative 4th month. In conclusion, AVP of leaflet extension technique offers an excellent early clinical result and represents a good alterna!ivy surgical treatment for the pure AR especially in young age group, although long-term follow-up is necessary to determine the durability of glutaraldehyde-preserved autologous pericardium as a valve leaflet.

• Journal of Chest Surgery
• /
• v.31 no.12
• /
• pp.1147-1158
• /
• 1998

Background: Portable cardiopulmonary bypass(CPB) technique has been used increasingly as a potent and effective option for emergency cardiopulmonary resuscitation(CPR) because it can maintain more stable hemodynamics and provide better survival than conventional CPR techniques. This study was designed to develop a prototype of Korean portable CPB system and, by applying it to CPR, to discriminate whether it would be superior to standard open-chest CPR. Material and Method: By using adult mongrel dogs, open-chest CPR(OCPR group, n=4) and portable-CPB CPR(CPB group, n=4) were compared with respects to restoration of spontaneous circulation(ROSC), hemodynamics, effects on blood cells, blood gas patterns, biochemical markers, and survivals. Ventricular fibrillation-cardiac arrest(VF-CA) of arrest(VF-CA) of 4 minutes followed by basic life support(BLS) of 15 minutes was applied in either group, which was standardized by the protocol of American Heart Association. Then, advanced life support(ALS) was applied to either group under the support of internal cardiac massage or CPB. ALS was maintained until ROSC was achieved but not longer than 30 minutes regardless of the presence of ROSC. All of the measured values were expressed as means±SD percent change from baseline. Result: During the early ALS, higher mean arterial pressure was maintained in CPB group than in OCPR group(90±19 vs. 71±32 %; p<.05) and lower mean pulmonary arterial pressure was also maintained in CPB group than in OCPR group(105±24 vs. 146±6%; p<.05). ROSC was achieved in all dogs. Post-ROSC levels of hematocrit, RBC, and platelet were decreased and plasma free hemoglobin was increased significantly in CPB group compared to OCPR group(p<.05). Changes in blood gas patterns, lactate, and CK-MB levels were not different between groups. Early mortality was seen in 3 dogs in OCPR group(survival time 31±36 hours) and 2 in CPB group(228±153 hours, p=ns). The remainders in both groups showed prolonged survival. Conclusion: These findings indicate that portable CPB can be effective to maintain stable hemodynamics during cardiac arrest, to achieve ROSC and to prolong survival. Further study is needed to refine the portable CPB system and to meet clinical challenges.

• Journal of Chest Surgery
• /
• v.42 no.4
• /
• pp.409-417
• /
• 2009

Background: Glutaraldehyde-fixed heterografts are prone to calcification after long-term implantation in human, and this is one of the limiting factors for the longevity of the heterografts used in cardiovascular surgery. The aim of the study was to evaluate the anticalcification effect of an ethanol and amino acids treatment on glutaraldehyde-fixed bovine pericardium. Material and Method: Bovine pericardial tissues were divided into 5 groups. Group 1 consisted of tissues fixed with glutaraldehyde, group 2 consisted of commercially available bovine pericardial valve tissues (Carpentier-Edwards PERIMOUNT), group 3 consisted of glutaraldehyde-fixed tissues treated with ethanol, group 4 consisted of glutaraldehyde-fixed tissues treated with ethanol and L-glutamic acid, and group 5 consisted of glutaraldehyde-fixed tissues treated with ethanol and homocysteic acid. The tissue microstructure was examined by light and electron microscopy. Tissue samples of each group were implanted into rat subcutaneous tissue for 3 $\sim$ 4 months and the calcium contents were measured after harvest. Result: The collagen fibers appeared to be well preserved in all the groups. The calcium contents of groups 2, 3, 4 and 5 (13.46$\pm$11.74, 0.33$\pm$0.02, 0.39$\pm$0.08 and 0.42$\pm$0.06 $\mu$g/mg, respectively) were all significantly lower than that of group 1 (149.97$\pm$28.25 $\mu$g/mg) (p<0.05). The calcium contents of groups 3, 4 and 5 were all significantly lower than that of group 2 (p<0.05). Conclusion: Treatment with ethanol alone or in combination with amino acids (L-glutamic acid or homocysteic acid) strongly prevented the calcification of glutaraldehyde-fixed bovine pericardium.

• Journal of Korea Water Resources Association
• /
• v.47 no.4
• /
• pp.371-384
• /
• 2014

This study developed a new algorithm of extreme rainfall extraction based on the Communication, Ocean and Meteorological Satellite (COMS) and the Tropical Rainfall Measurement Mission (TRMM) Satellite image data and evaluated its applicability for the heavy rainfall event in July-2011 in Seoul, South Korea. The power-series-regression-based Z-R relationship was employed for taking into account for empirical relationships between TRMM/PR, TRMM/VIRS, COMS, and Automatic Weather System(AWS) at each elevation. The estimated Z-R relationship ($Z=303R^{0.72}$) agreed well with observation from AWS (correlation coefficient=0.57). The estimated 10-minute rainfall intensities from the COMS satellite using the Z-R relationship generated underestimated rainfall intensities. For a small rainfall event the Z-R relationship tended to overestimated rainfall intensities. However, the overall patterns of estimated rainfall were very comparable with the observed data. The correlation coefficients and the Root Mean Square Error (RMSE) of 10-minute rainfall series from COMS and AWS gave 0.517, and 3.146, respectively. In addition, the averaged error value of the spatial correlation matrix ranged from -0.530 to -0.228, indicating negative correlation. To reduce the error by extreme rainfall estimation using satellite datasets it is required to take into more extreme factors and improve the algorithm through further study. This study showed the potential utility of multi-geostationary satellite data for building up sub-daily rainfall and establishing the real-time flood alert system in ungauged watersheds.

• Korean Journal of Soil Science and Fertilizer
• /
• v.21 no.4
• /
• pp.450-454
• /
• 1988

This study was conducted to increase the quality of Astrragali radix and to decide the application amounts of N, P and K fertilizer for Astragalus membranaceus Bunge. The ferlilizers effect on the ash contents and extracts from the root and the dried root yield was studied. With increasing the application amount of nitrogen more than 5kg/10a, the acid insoluble ash content of root increased, but the ethyl alcohol and diethyl ether extracts decreased. No effect of phosphorus amount on the ash and extracts contents in root was observed. With increasing amount more than 7kg/10a, only the acid insoluble ash contents increased. The dried root yield increased with the application level until 5kg/10a of nitrogen, but the effects ot phosphorus and potassium application were not observed. Positive correlation was obtained significantly between the ash ($r=0.746^*$) and acid insoluble ash ($r=0.858^{**}$) contents and the potassium application amount, and the negative correlation was also obtained significantly between the extract contents and the nitrogen ($r=-0.778^*$ ; $C_2H_5OH$, $r=-0.848^{**}$ ; $(C_2H_5O)_2O$) and potassium ($r=-0.772^*$ ; $(C_2H_5O)_2O)$ application amounts. As the results in the first year from the seeding, it is recommended for quality of Astragali radix to apply 5, 7-14, 7kg/10a of N, $P_2O_5$ and $K_2O$, respectively.

• Radiation Oncology Journal
• /
• v.17 no.1
• /
• pp.43-51
• /
• 1999

Purpose : To discuss the technical aspect of interstitial brachytherapy including method of implant, insertion time of radioactive source, total radiation dose, and complication, we reviewed patients who had diagnoses of soft tissue sarcoma and were treated by conservative surgery, interstitial implant and external beam radiation therapy Materials and Methods : Between May 1995 and Dec. 1997, ten patients with primary or recurrent soft tissue sarcoma underwent surgical resection (wide margin excision) and received radiotherapy including interstitial brachytherapy. Catheters were placed with regular intervals of 1 ~l.5 cm immediately after tumor removal and covering the critical structures, such as neurovascular bundle or bone, with gelform, muscle, or tissue expander in the cases where the tumors were close to those structures. Brachytherapy consisted of high dose rate, iridium-192 implant which delivered 12~15 Gy to 1 cm distance from the center of source axis with 2~2.5 Gy/fraction, twice a day, starting on 6th day after the surgery, Within one month after the surgery, total dose of 50~55 Gy was delivered to the tumor bed with wide margin by the external beam radiotherapy. Results : All patients completed planned interstitial brachytherapy without acute side effects directly related with catheter implantation such as infection or bleeding. With median follow up duration of 25 months (range 12~41 months), no local recurrences were observed. And there was no severe form of chronic complication (RTOGIEORTC grade 3 or 4). Conclusion : The high dose rate interstitial brachytherapy is easy and safe way to minimize the radiation dose delivered to the adjacent normal tissue and to decrease radiation induced chronic morbidity such as fibrosis by reducing the total dose of external radiotherapy in the management of soft tissue sarcoma with conservative surgery.