• 제목/요약/키워드: IPTG free-inducer

검색결과 1건 처리시간 0.019초

무-유도인자 단백질 발현 시스템을 이용한 재조합 시아노박테리아의 광합성 스쿠알렌 생산 평가 (Evaluation of Photosynthetic Squalene Production of Engineered Cyanobacteria Using the Chemical Inducer-Free Expression System)

  • 최선영;우한민
    • 한국미생물·생명공학회지
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    • 제49권3호
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    • pp.298-304
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    • 2021
  • Photosynthetic conversion through cyanobacteria and microalgae is an increasingly serious concern in the global warming crisis. Many value-added substances are produced through strain improvement, and much research and development is being conducted to determine its potential as an actual industrial strain. Economic barriers throughout processing production can be overcome to produce value-added chemicals by microalgal strains. In this study, we engineered cyanobacteria strains for the photosynthetic production of squalene and confirmed the continuous cultivation of CO2 and light conditions. The free-inducer system of gene expression was developed at the cyanobacterial strains. Then, the squalene production level and growth of the recombinant cyanobacteria were analyzed and discussed. For bio solar-cell factories, the ability to regulate genes based on the free-inducer gene expression system promotes metabolic engineering research and construction to produce value-added chemicals.