• BMB Reports
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• 제34권2호
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• pp.118-122
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• 2001

The translocation of ribose-binding protein (RBP) into the inverted membrane vesicles (IMV) of Escherichia coli and eukaryotic microsomes was studied using the in vitro translation/translocation system. It was found that RBP was translocated into heterologous eukaryotic microsomes co-translationally, as well as post-translationally However, RBP was translocated only past-translationally into IMV. Degradation fragments of RBP with the molar mass of 14 and 16 kDa were produced during the translocation into IMV However, the amount of the degradation products decreased and the mature form of RBP appeared in the presence of phenylmethylsulfonyl fluoride (PMSF). PMSF and GTP accelerated the translocation of RBF It was also found that SecB enhanced the post-translational translocation of RBP It appears that RBP is translocated via at least two targeting paths.

• 드라이브 ㆍ 컨트롤
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• 제16권2호
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• pp.30-35
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• 2019

EPPR (Electro-hydraulic Proportional Pressure Reducing) valves are pressure control valves. In this study, an independent metering valve (IMV), which is a combination of a spool valve opened and closed with the help of an EPPR valve, was discussed. The overall performance of the valve (IMV) was obtained by the respective modeling and simulation of the system. The valve investigated in this study is to be used for independent metering of hydraulic excavator actuator e.g. boom, arm, bucket etc. To design the model, continuity equations and force balance equations were used. The set of differential equations were then simulated in Simulink using ODE45 option in the configuration toolbox. The valve has to be able to control the flow rate going in and out of the cylinder separately, which is why the particular configuration was needed and selected.

• 드라이브 ㆍ 컨트롤
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• 제17권4호
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• pp.54-71
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• 2020

In light of the environmental challenges, energy-saving strategies are currently under investigation in the construction industry. This paper focuses on the energy-saving method used in the hydraulic system based on independent metering (IM) technologies, which can overcome the lost energy at the main control valve of the conventional electrohydraulic servo system. By scientifically arranging the proportional valves, the IM system can individually control the flow rate of the inlet and the outlet ports of the actuators. In addition, the IMV system can be used to effectively regenerate energy under different operating modes, thereby saving more energy than conventional hydraulic systems. Therefore, the IMV system has a great potential to improve the energy efficiency of hydraulic machinery. The overall IMV system, including the configuration, proportional valve, operation mode, and the control strategy is introduced via state-of-the-art hydraulic technologies. Finally, the challenges of IM systems are discussed to provide researchers with directions for future development.

• 한국동물생명공학회지
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• 제37권2호
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• pp.113-120
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• 2022

Sperm cryopreservation is a fundamental process for the long-term conservation of livestock genetic resources. Yet, the packaging method has been shown, among other factors, to affect the frozen-thawed (FT) sperm quality. This study aimed to develop a new mini-straw for sperm cryopreservation. In addition, the kinematic patterns, viability, acrosome integrity, and mitochondrial membrane potential (MMP) of boar spermatozoa frozen in the developed 0.25 mL straw, 0.25 mL (minitube, Germany), or 0.5 mL (IMV technologies, France) straws were assessed. Post-thaw kinematic parameters were not different (experiment 1: total motility (33.89%, 32.42%), progressive motility (19.13%, 19.09%), curvilinear velocity (42.32, 42.86), and average path velocity (33.40, 33.62) for minitube and the developed straws, respectively. Further, the viability (38.56%, 34.03%), acrosome integrity (53.38%, 48.88%), MMP (42.32%, 36.71%) of spermatozoa frozen using both straw were not differ statistically (p > 0.05). In experiment two, the quality parameters for semen frozen in the developed straw were compared with the 0.5 mL IMV straw. The total motility (41.26%, 39.1%), progressive motility (24.62%, 23.25%), curvilinear velocity (46.44, 48.25), and average path velocity (37.98, 39.12), respectively, for IMV and the developed straw, did not differ statistically. Additionally, there was no significant difference in the viability (39.60%, 33.17%), acrosome integrity (46.23%, 43.23%), and MMP (39.66, 32.51) for IMV and the developed straw, respectively. These results validate the safety and efficiency of the developed straw and highlight its great potential for clinical application. Moreover, both 0.25 mL and 0.5 mL straws fit the present protocol for cryopreservation of boar spermatozoa.

• 드라이브 ㆍ 컨트롤
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• 제15권4호
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• pp.119-124
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• 2018

• 드라이브 ㆍ 컨트롤
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• 제15권4호
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• pp.131-138
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• 2018

• 드라이브 ㆍ 컨트롤
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• 제15권4호
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• pp.102-107
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• 2018

• 드라이브 ㆍ 컨트롤
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• 제15권4호
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• pp.108-112
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• 2018

• 동력기계공학회지
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• 제20권3호
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• pp.36-42
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• 2016

A cylinder control system of the conventional construction machine has been controlled by hydraulic spool valves. This system is low-cost but system efficiency is not high. Recently, to improve this, all valves are controlled electronically and independently. Bu and Yao suggested four way electronic hydraulic control valve system. It is called IMVT(Independent Metering Valve Technology). The purpose of the study is to find proper IMV pressure control method for excavator and to validate excavator's bucket regeneration energy effect by controlling the IMV system. In this paper, we mathematically describe the bucket system of excavator first. And then, based on these results, we design the control system which is divided into two operations(none regeneration or regeneration).The results of the experiment show the desirable performance and usefulness of the designed control system.

• 드라이브 ㆍ 컨트롤
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• 제13권3호
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• pp.53-66
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• 2016

New non-hybrid approaches to improve energy efficiency of construction machineries are reviewed in this paper. Hydraulic systems are classified into four classes according to Backe's classification and commercially promising new technologies are carefully chosen in each class. IMV, 3-Line CPR, Closed Circuit Displacement Control of Differential Cylinder, and Throttle-less Secondary Control are chosen as representative non-hybrid new technologies. Key principle of each technology is explained and representative references which run through each technology are selected. Advantages and weaknesses of each technology are discussed and compared from the view point of construction machinery manufacturers.