• Journal of Computing Science and Engineering
• /
• v.3 no.2
• /
• pp.127-141
• /
• 2009

In this paper, we describe our vision of a "Just-in-time" initiative to solve the Data Interoperability Problem (a.k.a. INTEROP.) We provide an architectural overview of our initiative which draws upon existing technologies to develop an immersive and collaborative approach which aims at empowering data stakeholders (e.g., data producers and data consumers) with integrated tools to interact and collaborate with each other while directly manipulating visual representations of their data in an immersive environment (e.g., implemented via Second Life.) The semantics of these visual representations and the operations associated with the data are supported by ontologies defined using the Common Logic Framework (CL). Data operations gestured by the stakeholders, through their avatars, are translated to a variety of generated resources such as multi-language source code, visualizations, web pages, and web services. The generality of the approach is supported by a plug-in architecture which allows expert users to customize tasks such as data admission, data manipulation in the immersive world, and automatic generation of resources. This approach is designed with a mindset aimed at enabling stakeholders from diverse domains to exchange data and generate new knowledge.

• Korean Journal of Microbiology
• /
• v.32 no.4
• /
• pp.258-263
• /
• 1994

Genomic Southern hybridization of Frankia EuIKl strain, a nitrogen fixing symbiont of Elaeagnus umbellate root nodules, with nifH,D of K. pneumoniae as a probe, showed that 3.2 Kb and 5.5 Kb of BamHI fragments and 15 Kb PstI fragment were strongly hybridized with the probe, indicating nifH,D are located on these fragments. Using the same probe, one clone(pEuNIF) was isolated from the genomic library constructed into pWE15 cosmid vector by colony hybridization. The 3.2 Kb and 5.5 Kb BamHI fragments of this clone were hybridized with the same probe and this result corresponds to the genomic Southern hybridization data. However, using nifH of Frankia FaCl strain as a probe, only the 3.2 Kb BamHI fragment showed hybridization signal. Amino acid sequence deduced from nucleotide sequence of 3' terminus of the 3.2 Kb and 5' terminus of the 5.5 Kb fragments showed that the former was highly homologous with that of ArI3 nifD from 182nd to 240th amino acids, while the latter was from 241st to 282nd amino acids. These results show that nifH and partial nifD sequences are located on the 3.2 Kb fragment and residual sequences of nifH on the 5.5 Kb fragment which is contiguous to the 3.2 Kb fragment.

• Korean Journal of Agricultural Science
• /
• v.22 no.2
• /
• pp.156-162
• /
• 1995

This study was carried out to select the useful mutants among 20 lines treated with mutagen, gamma ray. These materials were treated with 15, 20, 25 and 30Krads, respectively and sowed at experimental field of College of Agriculture on 27th May, 1995. Items surveyed were germination rate and loss of chlorophyll at seedling stages, abnormal reproductive organs, discordance of flowering times and stem and ear height at ripening stages. The germination rate of lines treated with 15 Krad was different. The germination rate of the IK1, IK2 and Sinki lines were high, while the IK3, A632 and FR140 lines were very low. At same dose level, the germination rate of both IK1/H26 and Puyo synthetic variety were different; the germination rate of the IK1/H26 was high as above 70% at all treatments, while that of the Puyo synthetic variety was very poor at above 20 Krad. Generally stem adn ear height of the IK1/H26 were gradually decreased as the dose of mutagen increased, but there was no difference in stem and ear height of the IK1/H26 when this line was treated with 20Krad. The stem and ear height of lines treated with 15 Krad were shown to be decreased than those of check with non-treatment. The number of tiller of tillering lines were not greatly affected by ${\gamma}$-ray treatment.