• Title/Summary/Keyword: IIA

Search Result 448, Processing Time 0.025 seconds

The Relationship between Meat Color (CIE L* and a*), Myoglobin Content, and Their Influence on Muscle Fiber Characteristics and Pork Quality

  • Kim, Gap-Don;Jeong, Jin-Yeon;Hur, Sun-Jin;Yang, Han-Sul;Jeon, Jin-Tae;Joo, Seon-Tea
    • Food Science of Animal Resources
    • /
    • v.30 no.4
    • /
    • pp.626-633
    • /
    • 2010
  • This study examined the relationship between meat color and myoglobin content, and evaluated their influence on muscle fiber characteristics and overall pork quality. Four groups of pigs were classified by lightness (CIE $L^*$) and redness (CIE $a^*$): HH, high lightness and high redness; HL, high lightness and low redness; LH, low lightness and high redness; LL, low lightness and low redness. Myoglobin content ranged from 1.2 mg/g to 2.1 mg/g, and was highest in the LH group and lowest in the LL group (p<0.05). Myoglobin content correlated significantly with redness (CIE $a^*$) (r = 0.45, p<0.01). Fiber compositions of type I and IIA were closely related to lightness and redness. Pork with higher sizes of type IIA and IIB fibers had lower lightness and redness, respectively, which was tougher than the other pork. Pork having the highest lightness and lowest redness, often considered "pale", has higher values in drip loss than the other classes of pork tested.

인천국제공항(IIA)의 다짐시험시공 결과 및 적용

  • 김영웅;김용철
    • Geotechnical Engineering
    • /
    • v.14 no.5
    • /
    • pp.235-247
    • /
    • 1998
  • The IIA(Inchon International Airport) which will function as the HUB airport in the northeast Asian region in the upcoming 21th century will be located in the reclaimed land with sand dredged in the vicinity of project area between Youngjong and Yongyu islands. The original ground is composed of soft clayey silt (ML) or silty clay (CL). The reclaimed land is classified as being SP-SM and having poor gradation of Cu<3 which resulted in the anticipation for difficulty in compaction (compaction index = 0.6~0.7). This anticipation shedded light on the necessity of performing test compactions for the thickness of 3~5meters of reclaimed land, aiming at the discovery of effective and economical compaction method. Upon the call for the test compaction performance 4 different compaction methods have been selected for trial from the research done on the international and local academic papers, past experience with compaction works, and their written materials. For the precise interparetation of test results, the ground survey and measurements have been performed. The Hydraulic Hammer Compaction has been chosen as the most optimum in accordance withe the test results.

  • PDF

Cloning, Expression, and Nucleotide Sequencing of the Gene Encoding Glucose Permease of Phosphotransferase System from Brevibacterium ammoniagenes

  • Yoon, Ki-Hong;Yim, Hyouk;Jung, Kyung-Hwa
    • Journal of Microbiology and Biotechnology
    • /
    • v.8 no.3
    • /
    • pp.214-221
    • /
    • 1998
  • A Brevibacterium ammoniagenes gene coding for glucose/mannose-specific enzyme II ($EII^{Glc}$) of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned by complementing an Escherichia coli mutation affecting a ptsG gene, and the complete DNA nucleotide sequence was determined. The cloned gene was identified to be a ptsG, which enables the E. coli transportment to use glucose more efficiently than mannose as the sole carbon source in an M9 minimal medium. The ptsG gene of B. ammoniagenes consists of an open reading frame of 1,983 nucleotides putatively encoding a polypeptide of 661 amino acid residues and a TAA stop codon. The deduced amino acid sequence of the B. ammoniagenes $EII^{Glc}$ shows, at $46\%$, the highest degree of sequence similarity with the Corynebacterium glutamicum EII specific for both glucose and mannose. In addition, the $EII^{Glc}$ shares approximately $30\%$ sequence similarities with sucrose-specific and ${\beta}$-glucoside-specific EIIs of the several bacteria belonging to the glucose-PTS class. The 161-amino-acid C-terminal sequence of $EII^{Glc}$ is also similar to that of E. coli enzyme $IIA^{Glc}$, specific for glucose ($EIIA^{Glc}$). The B. ammoniagenes $EII^{Glc}$ consists of three domains; a hydrophobic region (EIIC) and two hydrophilic regions (EIIA, EIIB). The arrangement of structural domains, IIBCA, of the $EII^{Glc}$ is identical to those of EIIs specific for sucrose or ${\beta}$-glucoside. While the domain IIA was removed from the B. ammoniagenes $EII^{Glc}$ the remaining domains IIBC were found to restore the glucose and mannose-utilizing capacity of E. coli mutant lacking $EII^{Glc}$ activity with $EIIA^{Glc}$ of the E. coli mutant. $EII^{Glc}$ contains a histidine residue and a cysteine residue which are putative phosphorylation sites for the protein.

  • PDF

Estimation of Correlation Coefficients between Histological Parameters and Carcass Traits of Pig Longissimus Dorsi Muscle

  • Ryu, Y.C.;Rhee, M.S.;Kim, B.C.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • v.17 no.3
    • /
    • pp.428-433
    • /
    • 2004
  • The aim of this study was to investigate the histochemical parameters of muscle fibers, and to estimate the correlation between these histological parameters and carcass traits in pigs. A total of 230 crossbred Duroc$\times$(Yorkshire$\times$Landrace) pigs (149 gilts and 81 castrated male pigs) was evaluated. Carcass traits (carcass weight, backfat thickness, and loin eye area), muscle fiber size (crosssectional area, diameter, and perimeter), muscle fiber number (density of fibers/$mm^2$ and total number of fibers), and fiber type composition (percentages of myofibers and relative areas of each fiber type) were evaluated. Mean cross-sectional area (CSA) and type IIB fiber CSA were positively correlated to carcass weight, backfat thickness and loin eye area. Mean fiber CSA was mostly related to type IIB CSA (r=0.98) as a result of the high percentage of type IIB fibers in the longissimus muscle. Correlations between fiber diameters and perimeters were also high, and showed similar results with CSA. Mean fiber density was negatively correlated to carcass weight (r=-0.24), backfat thickness (r=-0.18) and loin eye area (r=-0.27). To the contrary, total fiber number was positively correlated with carcass weight (r=0.27) and loin eye area (r=0.53). Carcass weight and loin eyZe area were not significantly related to muscle fiber composition. For backfat thickness, there was an opposition between type IIA percentage, which was positively related and type IIB percentage, which was negatively related. Fiber type composition of type I and IIA fibers were negatively correlated to that of type IIB fibers (r=-0.67 to -0.74). In the present study, carcass weight and loin eye area were positively correlated to CSA and negatively correlated to fiber density. But, these relationships were generally low. The fiber density was strongly affected by muscle fiber size and the total fiber number was affected either by CSA of muscle fiber and loin eye area. Fiber type composition was much more related to their numerical abundance than their CSA.

Therapeutic Results of Postoperative Radiation Therapy for Early Stage Uterine Cervical Cancer (초기 자궁경부암의 수술후 방사선치료 결과)

  • Kang, Seung-Hee;Suh, Hyun-Suk
    • Radiation Oncology Journal
    • /
    • v.11 no.2
    • /
    • pp.347-354
    • /
    • 1993
  • This is a retrospective analysis of 67 patients with histologically proven invasive carcinoma of uterine cervix treated with surgery followed by adjuvant radiotherapy at Inje University Seoul Paik Hospital between october 1983 and september 1991, Postoperative radiotherapy was carried out in patients with high risks of locoregional recurrence such as positive pelvic lymph node (38 pts), large tumor size more than 3 cm (22 pts), cervical stromal invasion more than 2/3 (46 pts), parametrial involvement (9 pts), positive resection margin (14 pts), endo/myometrial extension (10 pts), and angiolymphatic invasion (13 pts). Stage I A, I B, and IIA were 2 $(3\%),$ 39 $(58.2\%),\;and\;26\;(38.8\%),$ respectively. Median follow-up period was 48 months with ranges from 13 to 115 months. All 67 patients were treated externally with standard pelvic field with radiation dose ranging from 4080 to 6120 cGy in 4~6 weeks period of time. Of these, 45 patients received intracavitary radiotherapy. The overall survival rate and disease free survival rate at 5-year were $88.0\%\;and\;82.1\%,$ respectively. The survival rates by stage were $87.1\%$ in IB and $88.4\%$ in IIA. Local control rate was $80.6\%(58\;pts).$ The treatment failure was noted in 12 of 67 patients $(17.9\%):$ locoregional failure in $7(10.4\%),$ distant metastasis in 3 $(4.5\%),$ and locoregional and distant metastasis in $2(3\%),$ The univariate analysis of prognostic factors disclosed endo/myometrial extension as a significant factor of survival and recurrence $(70.0\%\;vs\;91.1\%\;P<0.05\;&\;30.0\%\;vs\;15.8\%,\;respectively).$ The complication of postoperative radiothrapy was not significant and all patient were well tolerated. In conclusion, postoperative radiotherapy in patients with high risks of locoreginal recurrence is relatively well tolerated and it gives significantly improved survival rate especially in patients with positive lymph nodes, bulky tumor size $(\geqq3\;cm),$ parametrial involvement, cervical stromal invasion more than 2/3, positive resection margin and angiolymphatic invasion.

  • PDF

A Study on Construction Management System for Settlement in Korea through the Case of Program Management of IIA Project (IIA Project PM 적용사례를 통한 CM체계 정착방안)

  • Oh Myung-Kap
    • Proceedings of the Korean Institute Of Construction Engineering and Management
    • /
    • autumn
    • /
    • pp.96-101
    • /
    • 2001
  • Incheon International Airport Construction Project the biggest national multi-complex construction project in a single area in Korea, the necessity for introducing the Program Management System has been brought up from the development stage due to its specific characteristics and environment and with the Project Management Services Contract in December 1994 the system has been established and operated. At the time of contracting, there were controversies over the expected effect of the system since there were no related laws and regulations in domestic construction codes. Furthermore the situation was very hard in which some practical problems have been found in the process of applying and operating the system due to the lack of recognition and infrastructure in Korea. Incheon. In spite of these difficulties, thanks to the devotion and efforts of the project participants along with thoughtful concerns from the industries and the academies, the project goal has been successfully accomplished. This paper will analyze the various problems arose in the process of applying the Project Management System to Incheon International Airport and present more practical and desirable ways so that it can help to make the environment for properly settling Construction Management at the earliest.

  • PDF

New Roles of Glucose-Specific Enzyme IIA of the Vibrio vulnificus Phosphoransferase System

  • Kim, You-Jin;Koo, Byoung-Mo;Ryu, Yang-Kyun;Park, Soon-Jung;Lee, Kyu-Ho;Seok, Yeong-Jae
    • Proceedings of the Microbiological Society of Korea Conference
    • /
    • 2006.05a
    • /
    • pp.29-31
    • /
    • 2006
  • In a previous report, we showed that enzyme $IIA^{Glc}(EIIA^{Glc}$ of Escherichia coli phosphotransferase system (PTS) interacts with and regulates activity of FrsA (fermentation/respiration switch protein). A BLAST search revealed that orthologs of FrsA exist only in some Gram-negative bacteria such as E. coli, Salmonella typhimurium, Shigella flexneri, Yersinia pestis, Vibrio cholerae, Vibrio vulnificus, Vibrio parahemeolyticus, and Photorhabdus luminescens and all of these species are facultative anaerobes belonging to the ${\gamma}-proteobacterial$ group, and most of them are highly pathogenic. Ligand-fishing experiments using $EIIA^{Glc}$ of Vibrio vulnificus ($vEIIA^{Glc}$) as bait revealed that $vEIIA^{Glc}$ also interacts with vFrsA in a phosphorylation state-dependent manner. The frsA mutant of Vibrio vulnificus showed remarkably reduced cytotoxicity to HeLa cells and reduced lethality to mice compared to wild type. Comparison of extracellular proteomes between the mutant and wild type indicated that hemolysin was not produced in the frsA mutant. Characterization of another protein interacting with $vEIIA^{Glc}$ will be discussed.

  • PDF

An Adaptive Web Surfing System for Supporting Autonomous Navigation (자동항해를 지원하는 적응형 웹 서핑 시스템)

  • 국형준
    • Journal of KIISE:Software and Applications
    • /
    • v.31 no.4
    • /
    • pp.439-446
    • /
    • 2004
  • To design a user-adaptive web surfing system, we nay take the approach to divide the whole process into three phases; collecting user data, processing the data to construct and improve the user profile, and adapting to the user by applying the user profile. We have designed three software agents. Each privately works in each phase and they collaboratively support adaptive web surfing. They are IIA(Interactive Interface Agent), UPA(User Profile Agent), and ANA(Autonomous Navigation Agent). IIA provides the user interface, which collects data and performs mechanical navigation support. UPA processes the collected user data to build and update the user profile while user is web-surfing. ANA provides an autonomous navigation mode in which it automatically recommends web pages that are selected based on the user profile. The proposed approach and design method, through extensions and refinements, may be used to build a practical adaptive web surfing system.

Cloning, Nucleotide Sequencing, and Characterization of the ptsG Gene Encoding Glucose-Specific Enzyme II of the Phosphotransferase System from Brevibacterium lactofermentum

  • Yoon, Ki-Hong;Lee, Kyu-Nam;Lee, Jung-Kee;Park, Se-Cheol
    • Journal of Microbiology and Biotechnology
    • /
    • v.9 no.5
    • /
    • pp.582-588
    • /
    • 1999
  • A Brevibacterium lactofermentum gene coding for a glucose-specific permease of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) was cloned, by complementing an Escherichia coli mutation affecting a ptsG gene with the B. lactofermentum genomic library, and completely sequenced. The gene was identified as a ptsG, which enables an E. coli transformant to transport non-metabolizable glucose analogue 2-deoxyglucose (2DG). The ptsG gene of B. lactofermentum consists of an open reading frame of 2,025 nucleotides encoding a polypeptide of 674 amino acid residues and a TAA stop codon. The 3' flanking region contains two stem-loop structures which may be involved in transcriptional termination. The deduced amino acid sequence of the B. lactofermentum enzyme $II^{GIe}$ specific to glucose ($EII^{GIe}$) has a high homology with the Corynebacterium glutamicum enzyme $II^{Man}$ specific to glucose and mannose ($EII^{Man}$), and the Brevibacterium ammoniagenes enzyme $II^{GIc}$ specific to glucose ($EII^{GIc}$). The 171-amino-acid C-terminal sequence of the $EII^{Glc}$ is also similar to the Escherichia coli enzyme $IIA^{GIc}$ specific to glucose ($IIA^{GIc}$). It is interesting that the arrangement of the structural domains, IIBCA, of the B. lactofermentum $EII^{GIc}$ protein is identical to that of EIIs specific to sucrose or $\beta$-glucoside. Several in vivo complementation studies indicated that the B. lactofermentum $EII^{Glc}$ protein could replace both $EII^{ Glc}$ and $EIIA^{Glc}$ in an E. coli ptsG mutant or crr mutant, respectively.

  • PDF

A Study on Preference Heterogeneity of Economic Valuation for the Washland of Upo Wetland - Development of Waterfront Resources - (우포늪 천변저류지의 경제적 가치평가에 대한 선호이질성 연구 - 수변관광자원의 선택적 개발 -)

  • Yoo, Byong Kook;Kim, Hung Soo;Ju, Dug
    • Journal of Wetlands Research
    • /
    • v.15 no.3
    • /
    • pp.357-366
    • /
    • 2013
  • This study investigates to explain preference heterogeneity of respondents for economic valuation in washland of Upo wetland using Mixed Logit Model and Latent Class Model. Mixed Logit Model showed respondent heterogeneity in the attributes of wetland area and funds as well as some alternatives violated IIA assumption. 2-class Latent Class Model for respondents were used to explain the sources of the heterogeneity. Class 1 respondents who are located relatively close to Upo wetland had more experience and knowledge of Upo wetland and better understood the information suggested in the questionnaire than class 2 respondents in mostly metropolitan area of Seoul, Incheon.