• Title/Summary/Keyword: IIA

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Chemical constituents from the root of Dystaenia takeshimana (Nak.) Kitagawa

  • Jin, Wen-Yl;Seong, Nak-Sun;Na, Min-Kyun;An, Ren-Bo;Kang, Sam-Sik;Bae, Ki-Hwan
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.258.1-258.1
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    • 2003
  • The Dystaenia takeshimana (Nak.) Kitagawa is distributed in Ulreung island and it is an endemic species in korea. The MeOH extract from the root of D. takeshimana (Nak.) Kitagawa shows a good activity in anti-inflammatory assay (sPLA2IIA inhibition assay, COX-2 inhibition assay, 5-LO inhibition assay). (omitted)

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Temporal Expression of RNA Polymerase II in Porcine Oocytes and Embryos

  • Oqani, Reza;Lee, Min Gu;Tao, Lin;Jin, Dong Il
    • Reproductive and Developmental Biology
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    • v.36 no.4
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    • pp.237-241
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    • 2012
  • Embryonic genome activation (EGA) is the first major transition that occurs after fertilization, and entails a dramatic reprogramming of gene expression that is essential for continued development. Although it has been suggested that EGA in porcine embryos starts at the four-cell stage, recent evidence indicates that EGA may commence even earlier; however, the molecular details of EGA remain incompletely understood. The RNA polymerase II of eukaryotes transcribes mRNAs and most small nuclear RNAs. The largest subunit of RNA polymerase II can become phosphorylated in the C-terminal domain. The unphosphorylated form of the RNA polymerase II largest subunit C-terminal domain (IIa) plays a role in initiation of transcription, and the phosphorylated form (IIo) is required for transcriptional elongation and mRNA splicing. In the present study, we explored the nuclear translocation, nuclear localization, and phosphorylation dynamics of the RNA polymerase II C-terminal domain in immature pig oocytes, mature oocytes, two-, four-, and eight-cell embryos, and the morula and blastocyst. To this end, we used antibodies specific for the IIa and IIo forms of RNA polymerase II to stain the proteins. Unphosphorylated RNA polymerase II stained strongly in the nuclei of germinal vesicle oocytes, whereas the phosphorylated form of the enzyme was confined to the chromatin of prophase I oocytes. After fertilization, both unphosphorylated and phosphorylated RNA polymerase II began to accumulate in the nuclei of early stage one-cell embryos, and this pattern was maintained through to the blastocyst stage. The results suggest that both porcine oocytes and early embryos are transcriptionally competent, and that transcription of embryonic genes during the first three cell cycles parallels expression of phosphorylated RNA polymerase II.

Relationship between Myosin Isoforms and Meat Quality Traits in Pig Semitendinosus Neuromuscular Compartments

  • Graziotti, Guillermo H.;Menendez, Jose M. Rodriguez;Rios, Clara M.;Cossu, Maria E.;Bosco, Alexis;Affricano, Nestor O.;Ceschel, Alejandra Paltenghi;Moisa, Sonia;Basso, Lorenzo
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.1
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    • pp.125-129
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    • 2011
  • The aim was to determine the relationship between muscle structure and meat quality traits in neuromuscular compartments (NMCs: R1, R2, R3, R4) of pig semitendinosus muscle. Barrows from the INTA-MGC genetic line (Argentina) were slaughtered at 100 kg body weight. In each NMC the following parameters were determined: the fibre types I, IIA, IIX and IIB by immunohistochemistry, the fibre cross sectional area (FCSA), the pH of meat after 24 h post-mortem ($pH_{24}$), instrumental meat tenderness (WB) and colour ($L^*$, $a^*$, $b^*$). There were significant differences in the following: $L^*$ (R1 = R4$a^*$ (R1>R4>R2 = R3), $b^*$ (R1 = R4R1 = R3 = R4), $pH_{24}$ (R1 = R4>R2 = R3). The relative percentages of FCSA were as follows: I (R4>R1>R3>R2), IIA (R1>R4>R3>R2), IIX (R1 = R2 = R3 = R4) and IIB (R2>R3>R1>R4). The correlation values were statistically significant between IIB and WB (R1 and R4, $r_s$ = 0.66), (R2 and R3 $r_s$ = 0.74), IIB and $L^*$ (R1 and R4 $r_s$ = 0.84), IIX and $L^*$ without discriminating NMCs. Our data suggest that the NMC where the sampling takes place is important for determining meat quality traits because of the heterogeneity of the whole muscle.

Preparation of 27Ni6Zr4O143M(M=Mg, Ca, Sr, or Ba)O/70 Zeolite Y Catalysts and Hydrogen-rich Gas Production by Ethanol Steam Reforming

  • Kim, Dongjin;Lee, Jun Su;Lee, Gayoung;Choi, Byung-Hyun;Ji, Mi-Jung;Park, Sun-Min;Kang, Misook
    • Bulletin of the Korean Chemical Society
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    • v.34 no.7
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    • pp.2073-2080
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    • 2013
  • In this study the effects of adding alkaline-earth (IIA) metal oxides to NiZr-loaded Zeolite Y catalysts were investigated on hydrogen rich production by ethanol steam reforming (ESR). Four kinds of alkaline-earth metal (Mg, Ca, Sr, or Ba) oxides of 3.0% by weight were loaded between the $Ni_6Zr_4O_{14}$ main catalytic species and the microporous Zeolite Y support. The characterizations of these catalysts were examined by XRD, TEM, $H_2$-TPR, $NH_3$-TPD, and XPS. Catalytic performances during ESR were found to depend on the basicity of the added alkaline-earth metal oxides and $H_2$ production and ethanol conversion were maximized to 82% and 98% respectively in 27($Ni_6Zr_4O_{14}$)3MgO/70Zeolite Y catalyst at $600^{\circ}C$. Many carbon deposits and carbon nano fibers were seen on the surface of $30Ni_6Zr_4O_{14}$/70Zeolite Y catalyst but lesser amounts were observed on alkaline-earth metal oxide-loaded 27($Ni_6Zr_4O_{14}$)3MO/70Zeolite Y catalysts in TEM photos after ESR. This study demonstrates that hydrogen yields from ESR are closely related to the acidities of catalysts and that alkaline-earth metal oxides reduce the acidities of 27($Ni_6Zr_4O_{14}$)3MO/70Zeolite Y catalysts and promote hydrogen evolution by preventing progression to hydrocarbons.

Thymectomy for the Myasthemia Gravis Patient (중증 근무력증 환자의 흉선 절제술)

  • 정성운;박준호;김종원
    • Journal of Chest Surgery
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    • v.36 no.10
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    • pp.754-758
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    • 2003
  • Backgroun: Thymectomy was known as an effective treatment modality of myasthenia gravis. In this paper, we analyzed the result of thymectomy and the factors affecting the postoperative system improvement. Material and Method: We analyzed the medical records of 50 patients who received the thymectomy for myasthenia gravis from January 1997 to December 2001. Result: 39 patients showed sympton improvement. The effect of thymectomy as a treatment is 78%. There was no statistically significant correlation between postoperative improvement and Sex, Age, the Weight of thymic tissue, preoperative symptom duration, and preoperative mestinon dosage. However, the thymic pathology and low grade preoperative symptoms were affecting the postoperative prognosis. Conclusion: Thymic hyperplasia showed good prognosis compared to thymoma. Low grade preoperative symptoms (Group I or IIA) also showed good prognosis. So, early thymectomy is recommendable for the good treatment results of myasthenia gravis.

Expression and Morphology of Crystal Proteins in Bacillus thuringiensis subsp. kurstaki Cry-B

  • Park, Hyeon-U;Kim, Ho-San;Kim, Yeong-Hun;Jin, Byeong-Rae;Gang, Seok-Gwon
    • Journal of Sericultural and Entomological Science
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    • v.36 no.2
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    • pp.157-161
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    • 1994
  • To investigate the morphology of Bacillus thuringiensis crystal proteins, two type crystal protein genes, cryIA(c) gene under the control of cryIA(b) gene promoter and cryIIA gene under the control of its own promoter, were transformed in B. thuringiensis acrystalliferous mutant strain and the transformants were characterized by SDS-PAGE and scanning electron microscopy. The expression and formation of crystal proteins in B. thuringiensis subsp. kurstaki Cry-B revealed that crystal proteins appear to have same molecular weight and morphology to those of wild type strain's, suggesting that the expression and formation of crystal proteins affected not by host cell or recombination of cryIA(e) gene under the control of cryIA(b) gene promoter but by only structural fragment of protoxin.

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An Analysis on the Long-Term Variation of the GPS Broadcast Ephemeris Errors (GPS 방송궤도력 오차의 장기간 변화 분석)

  • Kim, Mingyu;Kim, Jeongrae
    • Journal of Advanced Navigation Technology
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    • v.18 no.5
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    • pp.421-428
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    • 2014
  • GPS satellite positions can be obtained from the navigation message transmitted from the GPS satellite. In this paper, the accuracy of broadcast orbit and clock are analyzed by comparing with the NGA precise ephemeris. For analyzing global and local orbit errors in 2004 to 2013, GPS satellite visibilities are calculated in Korea. Local RMS of 3D orbit error and SISRE are 4 cm and 3 cm less than global RMS of 3D orbit errors and SISRE. Orbit and clock errors are calculated for each GPS satellite Block for 10 years. SISRE of Block IIA satellites are 2.8 times greater than Block IIF satellites. The correlation between orbit errors and shadow condition is analyzed. The orbit errors in shadow is 2.1% higher than that in sunlight. Correlation analysis between the orbit errors and solar/geomagnetic index shows that orbit errors has a high correlation with from 2004 to 2008. However, the correlation became low since 2009.

Expression of Mutant p53 and MAGE-3 Gene Products in Esophageal Squamous Cell Carcinoma (식도 편평세포암종에서 변형 p53 단백과 MAGE-3 유전자 산물의 발현)

  • 조성래;양일종;이충석;전도환;장희경
    • Journal of Chest Surgery
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    • v.34 no.1
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    • pp.64-71
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    • 2001
  • 배경: 최근 치료법의 진보에도 불구하고 진행성 식도암의 예후는 5년 생존율이 10% 이하로매우 불량하기 때문에 식도암에 대한 새로운 치료방법의 하나로 암면역 치료가 대두되고 있다. 암면역 치료를 위해서 MAGE 등 종양 특이항원이 연구의 대상이 되고 있으나 국내에서는 아직 이에 대한 연구가 없다. 대상 및 방법: 1995년 1월부터 1998년 12월까지 고신대학교 복음병원 흉부외과에서 수술 치험한 125례의 식도암중 병리조직 보관상태가 양호한 편평세포암 79례를 병기에 따라(1병기 19례, IIa병기 19례, IIb병기 10례, III병기 21례, IV병기 10례) 무작위로 추출하고 대조군으로 평활근종 20례와 정상 식도점막 20례를 대조군으로 하여 DO7 단클론 항체와 항 MAGE-3 단클론 항체 57B를 이용하여 면역조직화학검사를 시행하여 변형 p53 단백과 MAGE-3 유전자 산물의 발현율을 조사하고 식도암 조직에서 질병의 진행도를 반영하는 병기에 따른 발현율 및 변형 p53 단백과 MAGE-3 유전자 산물의 발현율간의 상관관계를 조사하였다. 결과: 식도암조직에서 변형 p53 단백과 MAGE-3 유전자 산물의 발현율은 각각 51.9%, 60.8%의 발현율을 보였으나 식도평활근종과 정상 식도점막에서는 한례도 발현되지 않아 변형 p53 단백과 MAGE-3 유전자 산물은 대조군에 비해 식도암 조직에서 의미있게 발현되었다(p<0.001). 변형 p53 단백과 MAGE-3 유전자 산물의 발현은 I병기에서 68.4%, 52.6%, IIa병기에서 57.9%, 47.6%, IIb병기에서 60%, 70%, III병기에서 33.3%, 71.4%, IV병기에서 40%, 70% 각각 발현되어 병기에 따른 발현율의 차이는 없었다(p=0.193, p=0.452). 식도암 조직내에서 변형 p53 단백과 MAGE-3 유전자 산물의 발현간에는 상관관계가 없는 것으로 나타났다(p=0.679). 결론: 이상의 결과로 변형 p53 단백과 MAGE-3 유전자 산물의 발현은 식도암에서 예후인자로서의 역할은 할수 없으나 식도 편평세포 암조직에서만 특이하게 높은 빈도로 발현됨으로써 식도암도 면역치료의 대상이 될 수 있음을 확인하였다.

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