• The Korean Journal of Physiology
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• 제29권2호
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• pp.191-202
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• 1995

The purpose of this study was to compare effects of insulin and IGFs on growth, apical membrane enzyme activities and membrane transport systems of primary cultured rabbit kidney proximal tubule cells. Results were as follows: 1. Insulin and IGF-I produced significant growth stimulatory effects at $5{\times}10^{-10}M.\;IGF-II(5×10^{-10}\;M)$ did not stimulate significant cell growth. 2. Insulin stimulated the phosphorylation of a 97 KD protein. It was difficult to determine whether this band represents insulin and/or the IGF-I receptor. 3. The activities of apical membrane enzymes (alkaline phosphatase, leucine aminopeptidase, and ${\gamma}-glutamyl \;transpeptidase)$ were observed to be diminished after the cells were placed in the culture environment. 4. The uptake of ${\alpha}-MG,$ Pi and Na was significantly increased in cells incubated with insulin or IGF-I, IGF-II had no effect on the uptake of these substrates. 5. Na-pump activity, as assayed by Rb uptake, was significantly increased in cells treated with insulin or IGFs. In conclusion, insulin and IGF-I exert stimulatory effects on growth and membrane transporter(glucose, Na, Pi, and Na-pump) activities in primary cultured rabbit kidney proximal tubule cells. IGF-II had no effect on cell growth and membrane transporter(glucose, Na and Pi) activities.

• 한국식품영양과학회지
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• 제28권3호
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• pp.685-690
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• 1999

The insulin like growth factors(IGFs) are bound to several binding proteins(IGFBPs) that appear to regulate IGF transfort, receptor binding, and its action. The concentrations of these peptides are regulated by quantity and nutritional quality of dietary proteins. The aim of this study was to compare the effects of two diets, which differed in their protein source, Carassius carassius(CC), Carassius carassius hot water extract(CCHE), for 4 weeks. Body weight was significantly increased in the CC group(74.14$\pm$12.00 to 266.31$\pm$36.62g; p<0.01). Likewise, IGF I concentration of CC group(101.76$\pm$15.90 ng/ml) was significantly higher than that of CCHE group(38.50$\pm$ 11.20ng/ml; p<0.05). By western immunoblot analysis, especially IGFBP 1, 2 levels are increased, whereas IGFBP 3 level was de creased in CCHE group. After extraction of browning material from each samples, the extractive was filtered and absorbance at 420nm was measured. The absorbance of CCHE group was significantly higher than that of CC group. These results suggest that IGF I can be employed as an index of protein metabolism, particulary as a simple index in the assessing the status of protein nutrition.

• Tuberculosis and Respiratory Diseases
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• 제52권4호
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• pp.338-345
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• 2002

연구배경: 본 연구는 진폐증의 폐섬유화에 관여하는 사이토카인 중 PDGF-BB와 IGF-1의 혈청내 농도를 측정 비교함으로서 폐섬유화 관정에서의 역할을 간접적으로 확인하고 진폐증 진단의 생화학적 지표자로서 의미가 있는지 알아보고자, 충주의료원과 연세대학교 원주의과대학 원주기독병원에 내원한 환자를 대상으로 정상대조군과 단순형 석탄광부 진폐증군, 복잡형 석탄광부 진폐증군으로 나누어 혈청내 PDGF-BB와 IGF-1 농도를 측정하여 다음과 같은 결과를 얻었다. 방 법: 직업력과 방사선학적 소견으로 석탄광부 진폐증으로 진단된 환자중 단순형 석탄광부 진폐증 13예와 복잡형 석탄광부 진폐증 17예, 정상 대조군 10명을 대상으로 하였다. Human PDGF-BB immunoassay kit (R&D system, Minneapolis, MN)와 Human IGF-1 immunoassay kit (R&D system, Minneapolis, MN)를 이용하여 각 대상의 혈청내 PDGF-BB와 IGF-1의 농도를 측정하였다. 결 과: 1. 복잡형 석탄광부 진폐증군에서의 혈청 PDGF-BB 농도($10083.76{\pm}5639.07pg/mL$)가 정상 대조군 ($3726.17{\pm}1292.20pg/mL$)이나 단순형 석탄광부 진폐증($8493.88{\pm}5848.51pg/mL$)에 비해 통계학적으로 유의하게 높았다(P<0.05). 2. 정상대조군 ($413.40{\pm}61.94ng/mL$)과 단순형 석탄광부 진폐증($366.77{\pm}183.67ng/mL$), 복잡성 석탄광부 진폐증($403.40{\pm}115.39ng/mL$)의 혈청 IGF-1 농도는 각 군간 통계학적 유의한 차이는 관찰되지 않았다(P>0.05). 3. 작업년수에 따른 혈청 PDGF-BB와 IGF-1의농도는 통계학적으로 유의한 차이가 없었다(P>0.05). 결 론: 이상의 결과를 종합하여 보면 석탄광부 진폐증군에서 혈청내 PDGF-BB 농도의 증가는 폐섬유화 진행을 나타내는 생화학적 지표로서의 의미를 갖는 것으로 생각된다.

• 한국가정과학회지
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• 제9권1호
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• pp.81-88
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• 2006

IGFs and IGFBPs have an important role in controlling glucose homeostasis. This study was conducted to investigate the changes of insulin-like growth factor(IGF)-I. IGF-II and IGF binding proteins (IGFBPs) on fasting and postprandial state in Korean diabetes, Twenty eight healthy subjects and fifty seven diabetic patients participated in this study. The healthy subjects were not knowingly suffered from any disease and were not receiving any medical treatment, and diabetic subjects were undergo medical treatment, continuously. Weight and height were measured and body mass index (BMI) was calculated as weight (kg) divided by the square of height (m2). Blood pressure was measured. Plasma lipid profiles were analyzed by enzymatic methods, plasma Insulin and glucose levels were measured in fasting and postprandial state, respectively. The levels of serum IGFs and IGFBP-3 were measured by radioimmunoassay (RIA). The levels of glucose and insulin were significantly higher in diabetes than normal subjects on fasting as well as postprandial state (p<0.0l). The levels of IGF-I was significantly lower in diabetes than normal subjects, however in postprandial state, there was no significant difference between diabetes and control subjects, The levels of IGF-II were significantly lower in diabetes than control subjects both fasting and postpradial state, The level of IGFBP-3 were not significantly different between diabetes and normal subjects. Fasting IGF-I, IGF-II and IGFBP-3 levels were positively correlated with those levels on postprandial state, fasting IGe levels of IGF-I levels were positively correlated with fasting insulin levels, and postprandial IGF-I levels were positively correlated with fasting glucose, postprandial insulin and postprandial insulin levels, plasma triglyceride levels were correlated with plasma triglyceride levels. The IGFBP-3 levels were not correlated with IGF components, glucose, insulin and plasma lipids, These results demonstrate that in diabetes, the components IGF-I/IGFBPs system were significantly correlated with plsma glucose and insulin levels both fasting and postprandial state.

• 한국가축번식학회지
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• 제23권4호
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• pp.323-331
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• 1999

본 연구는 돼지난자의 체외성숙동안 insulin-like growth factor-I (IGF-I)과 난구세포의 영향을 검토하기 위하여 실시하였다. 미성숙난자를 0(60%), 1(61%), 5(62%) 및 l0ng/$m\ell$(72%)의 서로 다른 IGF-I의 농도로 첨가하여 배양했을 때 체외성숙율은 큰 차이를 나타내지 않았다. 또한 10ng/$m\ell$의 IGF-I이 첨가된 배양액내에서 난자를 배양한 경우, 난구세포부착시 (68%) 제거 (52%)된 난자에 비해 높은성숙율을 나타냈지만 유의적인 차이는 인정되지 않았다. 그러나 IGF-I이 첨가되지 않은 경우에는 난구세포 부착 (63%) 난자가 제거 (32%)된 난자에 비해 유의적 (P＜0.05)으로 높은 성숙율을 나타냈으며, IGF-I를 성숙 배양기간중 전반기 24시간 또는 후반기 24시간 동안만 첨가했을 때의 난구세포 부착시 (61과 49%) 제거된 (45 와 49%) 난자에 비해 높은 성숙융올 나타냈다. 한편, IGF-I의 존재 여부에 관계없이 FCS와 돼지난포액 (PFF)이 무첨가된 배양액에서 48시간동안 또는 배양전반기 24시간동안 난구세포를 제거한 경우 (16과 18%)로 난구세포 부착시 (46과 63%)에 비해 유의적 (P＜0.01)으로 낮은 성숙율을 나타냈다. 이와 같은 결과는 난구세포가 IGF-I의 존재 유무에 관계없이 난자의 체외성숙에 필수적이며, FCS와 PFF 첨가시 난구세포가 제거된 난자의 체외성숙을 촉진하는 것으로 밝혀졌다.

• Journal of Periodontal and Implant Science
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• 제30권2호
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• pp.389-405
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• 2000

The purpose of this study is to evaluate the effect of IGF-I for DNA synthetic activity and the mRNA expression of bone matrix protein, type I collagen and osteopontin in prolifetation and differentiation of MC3T3-E1 cells. To evaluate DNA synthetic activity, cells were seeded at $2{\times}10^4cells/ml$ in 24 well plates and to evaluate mRNA of type I collagen and osteopontin cells were seeded at $5{\times}10^5cells/ml$ in 100mm culture dishes. These cells were cultured in alpha-minimum essential medium(${\alpha}-MEM$) containing 10% fetal bovine serum at $37^{\circ}C$, 5% $CO_2$ incubator. For DNA synthetic activity test 1, 10, 100ng/ml IGF-I were added to the cells which had been cultured for 3 days before 24 hours. For type I collagen mRNA expression 1, 10ng/ml IGF-I were added to the cells which had been cultured for 5, 10 days and for osteopontin mRNA expression 0.1, 1, 10ng/ml IGF-I were added to the cells which had been cultured for 5, 15, 20 days. Cell proliferaton was measured by the incorporation of [$^3H$]-thymidine into DNA and expression for type I collagen and osteopontin were measured by northern blot analysis. The results were as follows : DNA synthetic activity were generally higher in experimental group than control group. Expressions of type I collagen mRNA were higher at 5 day group and much lower at 10 day group in the control groups. In the experimental groups, mRNA expressions were slightly increased when 1 ng/ml IGF-I were added to 5 day group and decreased in all experimental 10 day groups. Expressions of osteopontin mRNA were higher at 20 day groups and lower at 15 day groups than the control groups. In the experimental groups, mRNA expressions were incereased when 0.1, 1 ng/ml IGF-I were added to 5 day group and in all the 15 day groups, but decreased when 0.1, 1, 10 ng/ml IGF-I were added to 20 day groups. IGF-I stimulated DNA synthetic activity of MC3T3-E1 cells during proliferation stage significantly, did not greatly changed effects on type I collagen mRNA expression and stimulated osteopontin mRNA expression at 15 day especially. In conclusion, we suggests that IGF-I have a tendency of stimulation effect of DNA synthetic activity but do not stimulate type I collagen mRNA in proliferation stage of MC3T3-E1 cell cultures, and stimulate osteopontin mRNA in differentiation stage of MC3T3-E1 cell cultures.

• 대한의생명과학회지
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• 제23권1호
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• pp.39-43
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• 2017

In this study, we evaluated the effects of various hypothermic conditions ($32^{\circ}C$), including lithium chloride treatment, on insulin-like growth factor 1 (IGF-1) gene expression in PC12 cells. The results show that short-term hypothermic treatment (<1 day) resulted in relatively higher IGF-1 gene expression than did longer-term treatment (>1 day). Repeated switching between normal temperature and hypothermia every 2 h increased IGF-1 gene expression approximately 3-4-fold. These findings indicate that hypothermia dynamically regulates IGF-1 gene expression. This study could be helpful for the development of treatment and diagnostic strategies for ischemia.

• 한국가축번식학회지
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• 제22권4호
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• pp.341-347
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• 1998

본 연구는 생후 33 주령부터 초발정이 시작될 때까지 한우육성빈우의 일당증체량 (0.5kg, 0.7kg) 에 따른 혈중 progesterone 과 IGF-1 농도를 분석하여 한우의 초발정시기 및 춘기발동기전후 혈중 progesterone 과 IGF-1 농도를 비교 검토하기 위하여 수행하였다. 모든 시험축에서, 초발정 직전까지는 혈중 progesterone 농도는 검출할 수 없었으며, 13 두의 육성빈우에서 첫발정 6일전에 미미한 혈중 progesterone 농도가 상승하기 시작하여 짧은 황체기를 보였다. 초발정시기의 일당증체량 0.7kg 구의 평균 나이는 331.0$\pm$15.0 일로 0.5kg 구의 358.9$\pm$7.9 일보다 빨랐고 이때 체중도 0.7kg 구가 236.0$\pm$4.7kg으로 0.5kg 구의 224.8$\pm$9.7 보다 무거웠다. 초발정이후 3~15 일까지 IGF-1 농도는 황체초기 (0~1) 와 황체퇴행기 (18일~20일) 보다 높은 경향을 보였고, 성장단계에 따라서 생후 37주령부터 57주령까지 조사한 IGF-1 농도 변화는 37주령에 93.2$\pm$63.4ng/$m\ell$ 에서 57 주령에 170.9$\pm$59.35ng/$m\ell$로 점차적으로 상승하였다. 이상의 결과 한우의 초발정 평균나이는 345일령 전후로 추정되며 이시기에 혈중 progesterone 농도는 IGF-l 농도와 밀접한 관련이 있는 것 같다.

• Nutritional Sciences
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• 제3권2호
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• pp.57-65
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• 2000

Partial enteral nutrition (PEN) supplemented with insulin-like growth factor-I (IGF-I) to neonatal piglets receiving parenteral nutrition increases lactase-phlorizin hydrolase (LPH) activity, but not LPH mRNA. The goal of the current study was to investigate the mechanism by which IGF-I up-regulates LPH activity. We hypothesized that IGF-I regulates LPH synthesis post-transcriptionally. Methods: Newborn piglets (n=15) received 100% parenteral nutrition (TPN), 80% parenteral nutrition + 20% PEN (PEN), or PEN + IGF-I (1.0mg/kg/d). On day 7, two stable isotopes of leucine, [$^2 H_3$]-leucine and [$^{13}C_1$]-L-leucine were intravenously administered to measure mucosal protein and brush LPH (BB LPH) synthesis. Results: Weight gain, nutrient intake and jejunal weight and length were similar among the treatment groups. PEN increased mucosal weight, villus width and cross-sectional area, LPH activity, mRNA expression and the abundance of proLPHh compared to 100% TPN (p<0.05). IGF-I further increased mucosal weight, LPH activity and LPH activity per unit BB LPH ~2-fold over PEN alone (p<0.05), but did not affect LPH mRNA or the abundance of proLPHh or mature LPH. Isotopic enrichment of [$^2 H_3$]-leucine and [$^{13}C_1$]-L-leucine in plasma, mucosal protein and LPH precursors, and the fractional and absolute synthesis rates of mucosal protein and LPH were similar among the treatment groups. Total mucosal protein synthesis was increased 60% (p<0.05) and LPH synthesis tended (p=0.14) to be greater in the IGF-I treated animals compared to the other two groups. Conclusions: The primary mechanism by which IGF-I up-regulates LPH may be post-translational, either via reducing LPH turnover, or by specifically altering LPH activity.

• 한국가축번식학회지
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• 제24권1호
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• pp.35-40
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• 2000

본 연구는 Wistar계 rat를 이용하여 포유기간 중 제한포유시기와 이유시기를 조절하여, 혈중 IGF- I 수준변화에 대해 검토하였다. NL군 (NL)은 포유자의 수를 8 마리로 조절하였으며, RL군 (RL)과 W군 (W)은 RL0, RL0, RL10, RLl5 및 RL20, 그리고 W0, W5, WI0, W15 및 W20으로 각각 5개 군으로 구분하여, RL군은 각 개시일에 포유자의 수를 8마리에서 4마리로 조절하였으며, W군은 각 개시일에 포유자의 수를 완전히 이유시켜 다음과 같은 결과를 얻었다. 1. NL군의 혈중 IGF-I 농도는 분만직후 (Day 0) 750.59$\pm$3.52ng/$m\ell$ 을 나타내어 15일까지 일정한 수준을 유지하다가 그 후 포유 25일에 1,690.20$\pm$4.42ng/$m\ell$ 까지 증가되었다. 2. 포유초기 RL군 (RL0, RL5)의 IGF- I 농도는 포유 10일에 RL0군과 RL5군에서 각각 1,395.90$\pm$3.45 및 1,351.73$\pm$3.23ng/$m\ell$로서 NL 군의 745.96$\pm$2.24ng/$m\ell$ 보다 유의적 (P＜0.05)으로 높게 나타났으나 포유 15일부터는 차이가 없었다. 3. 포유초기 W군의 IGF- I 농도는 이유 후 일주일 정도는 NL군에 비해 높은 수준 (P＜0.05)을 나타내다가 10일경에 NL군과 같은 수준으로 감소하였다. 포유자극의 강약은 난소의 생리적 변화와 함께 혈중 IGF- I의 농도를 조절하는 것으로 사료된다.