• Title/Summary/Keyword: ICM

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Study on Epidermal Growth Factor (EGF) and Expression of EGF-Receptor (EGF-R) in Mouse IVF/IVC Embryo;I. Additive Effect of EGF and Expression of EGF-R on Mouse IVF Embryo Development (체외생산된 생쥐배에 대한 EGF와 EGF-R 발현에 관한 연구;I. 체외수정된 생쥐배 발달에 대한 EGF 첨가제 효과와 EGF-R 발현)

  • Kim, E.Y.;Uhm, S.J.;Kim, M.K.;Yoon, S.H.;Park, S.P.;Chung, K.S.;Lim, J.H.
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.1
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    • pp.13-20
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    • 1997
  • The objective of this study was to determine the effect of EGF on the preimplantation development of mouse IVF embryos and their ICM and TE cell number. And also, we examined the expression of EGF-R protein on embryonic development by indirect immunofluorescence. The results obtained in these experiments were summarized as follows; Group culture (5 embryos/ 25 ${\mu}l$) showed more improved development rate to blastocyst than singly culture. This inferior development of singly cultured 2-cell embryos improved by the addition of EGF. Especially, 2-cell embryos cultured singly in 10 ng/ml of EGF (62.4%) indicated significant difference in development to blastocyst compared with control group (47.9%). Also, cell number of ICM and TE by differential labelling showed the increased pattern in the EGF treatment group. The stimulating effect of EGF with the development level was significantly increased after 4-cell stage (p<0.05). ICM proportion also showed the increased pattern with the developmental level in the EGF treatment group. In addition, expression of EGF-R by indirect immunofluorescence detected after 4-cell stage. Therefore, EGF could stimulate preimplantation mouse embryo development by binding with expressed EGE-R after 4-cell stage and produce the more increased ICM and TE cell number of blastocyst.

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Study on the Additive Effect of Epidermal Growth Factor (EGF) and Expression of EGF-Receptor (EGF-R) on IVM/IVF Bovine Embryo Development (체외 생산된 소 수정란의 발달에 있어서 EGF 첨가제 효과와 EGF-R 발현에 관한 연구)

  • 김은영;김묘경;엄상준;윤산현;박세필;정길생;임진호
    • Korean Journal of Animal Reproduction
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    • v.20 no.3
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    • pp.279-288
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    • 1996
  • The objective of this study was to determine the effect of EGF on the development of IVM/IVF bovine embryos and their ICM and TE cell number. In addition, we examined the combined effect of EGF and coculture to the bovine embryo development and the expression of EGF-R protein on bovine embryos by indirect immunofluorescence. The results obtained in these experiments were summarized as follows: When the IVM/IVF 4- to 8-cell embryos were treated at 0, 1, 10, 100 ng/ml of EGF, EGF treatment group showed improved development to blastocyst and increased pattern of ICM and TE cell number compared with control, although there is not significantly different. The stimulating effect of EGF (10 ng/ml) to the develop ment level of IVM/IVF bovine embryos significantly increased development rate to blastocyst after 8-cell stage (p<0.05), although there is no significant effect to the increase of ICM and TE cell numbers. Also, expression of EGF-R on the bovine embryonic stage by indirect immunofluor escence presents after 4-cell stage and the intensity of the EGF-R staining was variable with the development progression. On the other hand, embryos cultured in coculture group added either with or without EGF commonly indicated the significant difference in development rate to blastocyst and Total cell number compared with control. These results suggest that the a addition of EGF to the coculture may stimulate the coculture effect between IVM/IVF bovineembryos and cumulus cells. Therefore, EGF could promote preimplantation bovine embryo development by binding with expressed EGF~R after 4-cell stage, and stimulate the production of embryotrophic factors from the coculture environment. Also, the present study showed that there was no significant effect of EGF to the increase of ICM and TE cell number although the rate of blastocyst significantly increased when treated with EGF after 8-cell stage (p<0.05).

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Establishment of Human Embryonic Stem Cells using Mouse Embryonic Fibroblasts and Human Fetal Fibroblasts as Feeder Cells (인간태아 섬유아세포와 생쥐배아 섬유아세포를 기저세포로 활용한 인간 배아줄기세포의 확립)

  • Cho, Hye Won;Ko, Kyoung Rae;Kim, Mi Kyoung;Lee, Jae Ik;Sin, Su Il;Lee, Dong Hyung;Kim, Ki Hyung;Lee, Kyu Sup
    • Clinical and Experimental Reproductive Medicine
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    • v.32 no.2
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    • pp.133-147
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    • 2005
  • Objectives: This study was carried out to establish human embryonic stem cells derived from frozen-thawed embryos using mouse embryonic fibroblasts (mEFs), human fetal skin and muscle fibroblasts as feeder cells, and to identify the characteristic of embryonic stem cells. Methods: When primary mEFs, human fetal skin and muscle fibroblasts were prepared, passaging on 4 days from replating could have effective trypsinization and clear feeder layers. Eight of 23 frozenthawed 4~8 cell stage embryos donated from consenting couples developed to blastocysts. Inner cell mass (ICM) was isolated by immunosurgery. ICM was co-cultured on mEFs, human fetal skin or muscle fibroblasts. The ICM colonies grown on mEFs, human fetal skin or muscle fibroblasts were tested the expression of stage specific embryonic antigen-3, -4 (SSEA-3, -4), octamer binding transcription factor-4 mRNA (Oct-4) and alkaline phosphatase surface marker. Results: We obtained 1 ICM colony from 2 ICM co-cultured on mEFs as feeder cells and did not obtain any ICM colony from 6 ICM clumps co-cultured on human fetal skin or muscle fibroblasts. The colony formed on mEFs could be passaged 30 times every 5 days with sustaining undifferentiated colony appearance. When the colonies cultured on mEFs were grown on human fetal skin or muscle fibroblasts, the colonies could be passaged 15 times every 9 days with sustaining undifferentiated colony appearance. The colonies grown on mEFs and human fetal fibroblasts expressed SSEA-4 and alkaline phosphatase surface markers and positive for the expression of Oct-4 by reverse transcription-polymerase chain reaction (RT-PCR). The produced embryoid body differentiated spontaneously to neural progenitorlike cells, neuron-like cells and beating cardiomyocyte-like cells, and frozen-thawed embryonic stem cells displayed normal 46,XX karyotype. Conclusions: The human embryonic stem cells can be established by using mEFs and human fetal fibroblasts produced in laboratory as feeder cells.

ICM from the foundation to the suspension of the old IMU (IMU탄생에서 해체까지의 ICM)

  • Kim, Sung-Sook;Khang, Mee-Kyung
    • Journal for History of Mathematics
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    • v.25 no.3
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    • pp.41-52
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    • 2012
  • The Great War of 1914-1918 had dramatic consequences for all aspects of European society. Academia, and the field of mathematics, was no exception to the changes which occurred following the conflicts conclusion. After the First World War, which left Germany, the Austro-Hungarian Empire, Bulgaria and Turkey defeated, the Treaty of Versailles imposed harsh revisions to the old order. Many new nations emerged and the map of Europe was redrawn. The victorious powers also created the International Research Council (IRC) in 1919, and the International Mathematical Union (IMU) was founded under the IRC' s umbrella in 1920. At that time Germany, Austria, Hungary and Bulgaria were excluded from participation and the IMU maintained an open anti-German policy. However, as time passed this policy became more sharply criticized and in 1928 ICM, the nonparticipants were invited to join. Having declined, controversy persisted until in 1931 the IRC was replaced by the International Council of Scientific Unions, and the IMU disappeared for over two decades until it was reestablished in 1951. During the time of the first tenure of the IMU it is argued by many that politics entered into the world of international mathematical cooperation. In this paper we study the real effects the Great War had on the international mathematical community and its mathematicians.

Long-term strength of shotcrete with improved C12A7 based mineral accelerator (개량형 C12A7계 광물계 급결제를 사용한 숏크리트의 장기강도 평가)

  • Won, Jong-Pil;Hwang, Un-Jong;Lee, Su-Jin;Lee, Jae-Ho;Jang, Seok-Bu
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.16 no.2
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    • pp.135-148
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    • 2014
  • This study was performed to evaluate the performance about Improved C12A7 based mineral accelerator (ICM) increased in initial and long-term strength. ICM was developed to overcome the long-term strength decrease in existing accelerator. To evaluate the performance of ICM according to addition rate, setting time, compressive strength, and flexural strength tests were conducted in laboratory. In results, initial setting time was slower, final setting time was faster than existing $C_{12}A_7$ based mineral accelerator (CM) when usage of ICM 6%. In compressive and flexural strength, existing CM was higher than ICM at 3hours and 1day. After 7days, strength of shotcrete using ICM was increased. Rebound test, compressive strength and flexural strength test with optimum addition rate through the laboratory test were conducted in field. Field experiment results were the same as laboratory test. Long-term strength performance of ICM was superior to existing accelerator.

Parthenogenetic Mouse Embryonic Stem Cells have Similar Characteristics to In Vitro Fertilization mES Cells (체외수정 유래 생쥐 배아줄기세포와 유사한 특성을 보유한 단위발생 유래 생쥐 배아줄기세포)

  • Park, Se-Pill;Kim, Eun-Young;Lee, Keum-Si;Lee, Young-Jae;Shin, Hyun-Ah;Min, Hyun-Jung;Lee, Hoon-Taek;Chung, Kil-Saeng;Lim, Jin-Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.29 no.2
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    • pp.129-138
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    • 2002
  • Objective: This study was to compare the characteristics between parthenogenetic mES (P-mES) cells and in vitro fertilization mES cells. Materials and Methods: Mouse oocytes were recovered from superovulated 4 wks hybrid F1 (C57BL/6xCBA/N) female mice. For parthenogenetic activation, oocytes were treated with 7% ethanol for 5 min and $5{\mu}g$/ml cytochalasin-B for 4 h. For IVF, oocytes were inseminated with epididymal sperm of hybrid F1 male mice ($1{times}10^6/ml$). IVF and parthenogenetic embryos were cultured in M16 medium for 4 days. Cell number count of blastocysts in those two groups was taken by differential labelling using propidium iodide (red) and bisbenzimide (blue). To establish ES cells, b1astocysts in IVF and parthenogenetic groups were treated by immunosurgery and recovered inner cell mass (ICM) cells were cultured in LIF added ES culture medium. To identify ES cells, the surface markers alkaline phosphatase, SSEA-1, 3,4 and Oct4 staining were examined in rep1ated ICM colonies. Chromosome numbers in P-mES and mES were checked. Also, in vitro differentiation potential of P-mES and mES was examined. Results: Although the cleavage rate (${\geq}$2-cell) was not different between IVF (76.3%) and parthenogenetic group (67.0%), in vitro development rate was significantly low in parthenogenetic group (24.0%) than IVF group (68.4%) (p<0.05). Cell number count of ICM and total cell in parthenogenetic b1astocysts ($9.6{\pm}3.1,\;35.1{\pm}5.2$) were signficantly lower than those of IVF blastocysts ($19.5{\pm}4.7,\;63.2{\pm}13.0$) (p<0.05). Through the serial treatment procedure such as immunosurgery, plating of ICM and colony formation, two ICM colonies in IVF group (mES, 10.0%) and three ICM colonies (P-mES, 42.9%) in parthenogenetic group were able to culture for extended duration (25 and 20 passages, respectively). Using surface markers, alkaline phosphatase, SSEA-l and Oct4 in P-mES and mES colony were positively stained. The number of chromosome was normal in ES colony from two groups. Also, in vitro neural and cardiac cell differentiation derived from mES or P-mES cells was confirmed. Conclusion: This study suggested that P-mES cells can be successfully established and that those cell lines have similar characteristics to mES cells.

Morphological Characteristics of Pig Blastocysts Produced by Somatic Cell Nuclear Transfer

  • Y.M. Han;D.B. Koo;Park, Y.H.;Park, J.S.;Kim, H.N.;Y.K. Kang;W.K. Chang;Lee, K.K.
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.68-68
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    • 2001
  • Blastocyst formation, consisting of the inner cell mass (ICM) and trophectoderm (TE), is the first differentiation process during embryonic development in mammals. It has been hypothesized that the proportion of ICM to TE in the blastocyst may be crucial for subsequent developmental competence of early embryos, which it may be expressed as a sensitive indicator for evaluating in vitro systems. In this study ICM/total cell ratio of nuclear transfer (NT) embryos was compared with IVF-derived and in vivo embryos. Somatic cell nuclei obtained from a fetus at Day 40 of gestation were transferred into the enucleated oocyte and then cultured in NCSU 23 medium for 6 days as previously described (Koo et al., Biol. Reprod. 2000; 63:986-992). ICM and TE cells of blastocysts were determined by using a differential staining method (Han et al., Biol. Reprod. 1999; 60:1110-1113). Development rate (9.8$\pm$2.5%, 23/225) to the blastocyst stage of NT embryos was lower than IVF embryos (23.8$\pm$2.7%, 53/223). Thus, a difference was detected in the in vitro developmental rate to blastocyst stage between NT and IVF-derived embryos (P<0.05). In the next experiment, we investigated ICM and TE nuclei to assess the quality of blastocysts that produced by NT, IVF and in vivo, respectively. NT blastocysts (27.6$\pm$8.3) showed a smaller total cell number than IVF-derived (42.6$\pm$17.4) and in vivo embryos (283.9$\pm$103.5) (P<0.05). Ratios of ICM/total cells in NT, IVF and in vivo blastocysts were 15.1$\pm$ 18.6% (n=56), 12.3$\pm$9.2% (n=57) and 30.4$\pm$6.8% (n=40), respectively. Individual blastocysts for the ratio of ICM/total cells were assigned to 3 groups (I; <20%, II; 20 to 40% and III;>40%). As the results, most in vivo blastocysts (97.5%, 39/40) were distributed into group II while most NT (78.6%, 44/56) and IVF-derived blastocysts (82.5%, 47/57) were allocated to group I. Thus, our data show that NT or IVF-derived embryos have aberrant morphology during early development in vitro systems, suggesting that these anomalies may result in developmental failures of the NT embryos to term.

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A Study on the Improvement Plan of Information Process Model in C4ISR Effectiveness Analysis Model DNS (C4ISR효과분석모델 DNS의 정보처리모델 개선 방안 연구)

  • Lee, Kwang-Myoung;Hong, Yoon-Gee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.13 no.4
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    • pp.1822-1829
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    • 2012
  • In this research, we developed the Information Circulation Model(ICM) by modifying and supplementing Information Process Model(IPM) in DNS. This ICM is used to simulate some combat situations that could not be considered with the existing DNS. We showed that this improved ICM can be applied to simulation and analysis of a variety of interests compared with IPM in DNS. We expect this study could be a basic research for further development of C4ISR effectiveness analysis in our national defense community.

Dual effects of ram pressure on star formation in multiphase disk galaxies with strong stellar feedback

  • Lee, Jaehyun;Kimm, Taysun;Katz, Harley;Rosdahl, Joakim;Devriendt, Julien;Slyz, Andrianne
    • The Bulletin of The Korean Astronomical Society
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    • v.46 no.1
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    • pp.28.2-28.2
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    • 2021
  • We investigate the impact of ram pressure stripping due to the intracluster medium (ICM) on star-forming disk galaxies with a multiphase interstellar medium maintained by strong stellar feedback. We carry out radiation-hydrodynamic simulations of an isolated disk galaxy embedded in a 1011 M⦿ dark matter halo with various ICM winds mimicking the cluster outskirts (moderate) and the central environment (strong). We find that both star formation quenching and triggering occur in ram pressure-stripped galaxies, depending on the strength of the winds. HI and H2 in the outer galactic disk are significantly stripped in the presence of moderate winds, whereas turbulent pressure provides support against ram pressure in the central region, where star formation is active. Moderate ICM winds facilitate gas collapse, increasing the total star formation rates by ~40% when the wind is oriented face-on or by ~80% when it is edge-on. In contrast, strong winds rapidly blow away neutral and molecular hydrogen gas from the galaxy, suppressing star formation by a factor of 2 within ~200 Myr. Dense gas clumps with nH≳10 M⦿ pc-2 are easily identified in extraplanar regions, but no significant young stellar populations are found in such clumps. In our attempts to enhance radiative cooling by adopting a colder ICM of T=106K only a few additional stars are formed in the tail region, even if the amount of newly cooled gas increases by an order of magnitude.

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