• Journal of Biosystems Engineering
• /
• v.21 no.4
• /
• pp.414-421
• /
• 1996

한국과 일본의 경우 건표고를 외관의 품질상태 에 따라 12등급에서 16등급으로 구분하고 있다. 그리고 등급판정 작업은 임의로 추출한 샘플을 대상으로 전문 감정가에 의해 수작업으로 수행되고 있다. 건표고의 품질을 결정짓는 외관의 품질인자들은 갓과 내피에 고루 분포하고 있다. 본 논문에서는 컴퓨터 영상처리 시스템에 의거하여 개발한 건표고 자동 등급판정 및 선별 시작시스템의 구조와 기능 그리고 성능에 대하여 설명하였다. 개발한 시작시스템은 표고의 이송과 취급자동화를 위한 진동이송기, 반전장치, 컨베이어 이송장치와 두 세트의 컴퓨터 영상처리 시스템, 그리고 시스템 통괄제어를 위한 IBM PC AT호환 컴퓨터, 디지털 입출력 보드, 전공압실린더 구동제어를 위한 PLC등으로 구성하였다. 등급판정의 효율성 및 실시간 작업시스템을 고려하여 건표고의 등급판정은 두 세트의 컴퓨터 영상처리 시스템을 이용하여 이송되는 건표고의 갓 또는 내피 중 어디가 위를 향하는 지에 따라 두 단계에 걸쳐 독립적으로 판정을 수행하도록 하였다. 첫 번째 영상처리부에서는 갓표면 영상으로부터 4등급의 고품질 표고를 분류하며 두 번째 영상처리부에서는 내피표면 영상으로부터 중간 및 저품질 표고를 8개의 등급으로 분류한다. 실시간 영상정보처리를 목적으로 기존에 개발한 신경회로망을 이용한 등급판정 알고리즘을 시작시스템에 적용하였다. 개발한 시작기는 88% 이상의 등급판정 정확도를 보여 주었으며, 전공압시스템의 구동제약으로 인하여 표고 1개당 약0.7초의 선별시간이 소요되었다. 일조 선별라인의 경우 본 연구에서 제안한 시작기의 선별능력은 표고가 일차 처리부로 갓이 위로 올라와 있는 상태로 계속 공급된다면 시간당 대략 5,000여 개의 표고를 처리할 수 있을 것으로 기대된다.보강하여 가능하면 B-Pillar의 Middle이 Bending type collapse을 방지하여 Pelvis와 Door가 먼저 접촉하는 방법 등이 적용가능하다. 제작하기 이전에 설계된 부품에 대한 스프링 상수 및 내구특성을 체계적으로 규명하여 제품 시험의 횟수를 줄이고, 보다 정밀한 제품을 제작할 수 있도록 하기 위한 것이다.세포수는 초기 배반포기배에서 팽윤 배반포기배로 진행됨에 따라 두배에서 세배 정도 증가되었음을 알 수 있었다. 또한, differential labelling과 bisbenzimide기법에서 얻어진 각각의 총세포수를 비교하였을 때 총세포수는 발달의 진행 정도에 따라 증가되며 그와 동시에 동일한 군 간의 세포수도 거의 유사함을 알 수 있었다. 따라서, ICM과 TE를 differential labelling하는 기법은 수정란의 quality를 평가하는데 매우 유용한 기법으로서 착상전 embryo 발달을 연구하는데 효과적으로 이용될 수 있다는 것을 시사한다. 고도의 유의차를 나타낸 반면 비수구, 초생수로구 및 Bromegrass 목초구 간에는 아무런 유의차가 인정되지 않았다. 7. 농지보전 처리구인 배수구와 초생수로구는 비처리구에 비해 낮은 침두 유출량과 낮은 토양유실량을 나타내었다.구보다 14% 절감되는 것으로 나타났다.작용하는 것으로 사료된다.된다.정량 분석한 결과이다. 시편의 조성은 33.6 at% U, 66.4 at% O의 결과를 얻었다. 산화물 핵연료의 표면 관찰 및 정량 분석 시험시 시편 표면을 전도성 물질로 증착시키지 않고, Silver Paint 에 시편을 접착하는 방법으로도 만족한 시험 결과를 얻을 수 있었다.째, 회복기 중에 일어나는 입자들의 유입은 자기폭풍의 지속시간을 연장시키는 경향을 보이며 큰

• Reproductive and Developmental Biology
• /
• v.34 no.3
• /
• pp.127-134
• /
• 2010

This study was to investigate the effect of flavonoid treatment on in vitro development of bovine somatic cell nuclear transfer (SCNT) embryos, and their pregnancy and delivery rate after embryo transfer into recipient. In experiment 1, to optimize the flavonoid concentration, parthenogenetic day 2 ($\geq$ 2-cell) embryos were cultured in 0 (control), 1, 10 and $20\;{\mu}M$ flavonoid for 6 days. In the results, in vitro development rate was the highest in $10\;{\mu}M$ flavonoid group (57.1%) among treatment groups (control, 49.5%; $1\;{\mu}M$, 54.2%; $20\;{\mu}M$, 37.5%), and numbers of total and ICM cells were significantly (p<0.05) higher in $10\;{\mu}M$ flavonoid group than other groups. We found that $10\;{\mu}M$ flavonoid treatment can significantly (p<0.05) decrease the apoptotic index and derive high expression of anti-oxidant, anti-apoptotic, cell growth and development marker genes such as Mn-SOD, Survivin, Bax inhibitor, Glut-5, In-tau, compared to control group. In experiment 2, to produce the cloned Jeju Black Cattle, beef quality index grade 1 bull somatic cells were transferred into enucleated bovine MII oocytes and reconstructed embryos were cultured in $10\;{\mu}M$ flavonoid added medium. When the in vitro produced day 7 or 8 SCNT blastocysts were transferred into a number of recipients, $10\;{\mu}M$ flavonoid treatment group presented higher pregnancy rate (10.2%, 6/59) than control group (5.9%, 2/34). Total three cloned Jeju Black calves were born. Also, two cloned calves in $10\;{\mu}M$ flavonoid group were born and both were all healthy at present, while the one cloned calf born in control group was dead one month after birth. In addition, when the result of short tandem repeat marker analysis of each cloned calf was investigated, microsatellite loci of 11 numbers matched genotype between donor cell and cloned calf tissue. These results demonstrated that the flavonoid addition in culture medium may have beneficial effects on in vitro and in vivo developmental capacity of SCNT embryos and pregnancy rate.

• Proceedings of the KSAR Conference
• /
• 2003.06a
• /
• pp.56-56
• /
• 2003

Beta-catenin is very important in early development including involvement in cell adhesion, cell signaling, and developmental fate specification. Cell-cell interaction is an important process during mammalian embryonic development. In preimplantation embryos, embryonic compaction is the process of increased cellular flattening and adhesion of junctional complexes and results in a polarized distribution. Beta-catenin is associated with embryonic compaction in mammals. Here, we examined the relationship between beta-catenin expression and compaction in porcine embryos derived from in vitro fertilization. First of all, we investigated beta-catenin expression in each embryonic developmental stage and also focused on expression pattern according to full, partial and non-compaction at morula stage. We used the immunocyto-chemical method in this research. To confirm compaction affects on the embryonic development, we compared between compaction and developmental rates to the blastocyst. The result showed that compaction and non-compaction rates were 14.6% and 63.8% at 4 days after IVF, respectively The developmental rates to the blastocyst and their total cell number were 50.9% vs 36.4% and 41.4$\pm$11.5 vs 26.8$\pm$12.7 in compaction and non-compaction groups. Although no difference was detected in the ratio of ICM to total cells between two groups, total cell number of the blastocysts in compaction group was superior to that of the blastocysts in non-compaction group (P<0.05). Expression of beta-catenin appeared in the boundary of membrane surface between blastomeres in 2- and 4-cell stage, and observed irregular pattern from 8-cell to blastocyst stage. We also investigated beta-catenin expression pattern according to the degree of compaction in the 3 groups; full, partial (>50%) and non-compaction. The expression signal in fully compacted embryos was stronger than those of partial and non-compacted embryos. Especially, beta-catenin expression appeared various patterns in morula stage suggesting the aberrant distribution of beta-catenin is affected by compaction patterns. Our results suggest that abnormal beta-catenin expression was affected by embryo quality and further development in porcine embryos in vitro.

• Reproductive and Developmental Biology
• /
• v.35 no.3
• /
• pp.307-311
• /
• 2011

Heat shock protein 90 (Hsp90) is ATPase-directed molecular chaperon and affects survival of several cells. In our previous study, inhibitory effect of Hsp90 by inducing cell cycle arrest and apoptosis in the pig embryonic and primary cells was reported. However, its role during early bovine embryonic development is not sufficient. In this study, we traced the effects of Hsp90 inhibitor, 17-allylamino-17-demethoxygeldanamycin (17-AAG), on early bovine embryonic development. We also investigated several indicators of developmental potential, including structural integrity, gene expression (apoptosis-related genes), and apoptosis, which are affected by 17-AAG. Bovine embryos were cultured in the CR1-aa medium with or without 17-AAG for 7 days. In result, significant differences in developmental potential were detected between the embryos that were cultured with or without 17-AAG ($33.1{\pm}9.6$ vs $21.7{\pm}8.3%$). The structural integrity of the blastocysts was examined by differential staining. Blastocysts from the dbcAMP-treated group had higher numbers of ICM, TE, and total cells than those from the untreated group. Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) showed that the number of containing fragmented DNA at the blastocyst stage increased in the 17-AAG treated group compared with control (11.2 vs 3.9, respectively). Blastocysts that developed in the 17-AAG treated group had low structural integrity and high apoptotic nuclei than those of the untreated control, resulting in decrease the embryonic qualities of preimplantation bovine blastocysts. The mRNA expression of the pro-apoptotic gene (Bax) increased in 17-AAG treated group, whereas expression of the antiapoptotic gene (Bcl-XL) decreased. In conclusion, Hsp90 also appears to play a direct role in bovine early embryo developmental competence including structural integrity of blastocysts. Also, these results indicate that Hsp90 is closely associated with apoptosis-related genes expression in developing bovine embryos.

• Journal of Embryo Transfer
• /
• v.29 no.2
• /
• pp.101-109
• /
• 2014

Matrix Metalloproteinases (MMP)-2 and -9 are participated in embryo development, implantation, remodeling of epithelial cell and ovulation. The objective of this study is to evaluate an impact of MMP2 and MMP9 on embryonic developmental competence as well as gene expression profiles of in vitro-produced bovine embryos. After in vitro fertilization, embryos of all groups were transferred into IVC-2 medium treated with MMP2 and MMP9 to check the optimum concentration on the basis of embryo development competence and cell numbers. The optimum concentrations for MMP2 and 9 were 1,200 ng/ml and 300 ng/ml. The blastocyst development competence was not different among 1,200 ng/ml of MMP2 vs. 300 ng/ml of MMP9 vs. combined MMP2 + 9 vs. control groups ($41.46{\pm}10.66$ vs. $37.73{\pm}8.92$ vs. $45.11{\pm}11.41%$ vs. $41.59{\pm}11.88$, respectively). Furthermore, the developmental competences to hatching and hatched blastocysts were not also different among the same groups ($79.84{\pm}12.63$ vs. $83.3{\pm}17.46$ vs. $78.55{\pm}14.48%$ vs. $72.02{\pm}14.09$). In addition, total cell number was significantly (p<0.05) greater in blastocyst treated with MMP9 300 ng/ml among all treatment groups. On the other hand, there was no significant difference of ICM vs. TE ratio in all groups. The expression of five out of six genes (i.e., MMP2, MMP9, IFNt, SSLP1 and HNRNPA2B1) was different among the groups. The expression of IFNt and HNRNPA2B1 genes was significantly greater in MMP9 (p<0.05), but there was no difference of MMP9 expression between MMP2 and MMP9 group (p>0.05). The normalized expression of MMP2 and SSLP1 was greater in MMP2 than other groups (p<0.05). In conclusion, MMPs treatment during IVC-2 medium was remarkably effected on blastocyst developmental competence and gene expression profiles that are related to embryo quality and implantation.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.10
• /
• pp.1510-1516
• /
• 2007

These experiments were carried out to optimize the parameters of electrical activation, methods of parthenogenetic activation and embryo culture in vitro and meanwhile to isolate embryonic stem cells-like (ESCs) derived from porcine parthenogenetic blastocysts (pPBs). These results showed that, as the electric field strength increased from 1.0 to 2.7 kV/cm, the cleavage rate of parthenogenetic embryos increased gradually but the rate of oocyte lysis was significantly increased when using 2.7 kV/cm field strength. The rate of cleavage in 2.2 and 2.7 kV/cm groups was significantly increased in comparison with that of the 1.0 kV/cm group. A voltage field strength of 2.2 kV/cm DC was used to investigate blastocyst development following activation with a single pulse of 30 or $60-{\mu}sec$ pulse duration. The optimum pulse duration was 30-${\mu}sec$, with a blastocyst rate of 20.7%. Multiple pulses were inferior to a single pulse for blastocyst yield (8.0% vs. 29.9) (p<0.05). For porcine oocyte parthenogenetic activation methods, the rates of cleavage (79.0% vs. 59.8%) and blastocysts (19.4% vs. 3.4%) were significantly increased in electrical activation in contrast to chemical activation with ionomycin/6-DMAP (p<0.05). Rates of cleavage and blastocyst formation in NCSU-23 and PZM-3 embryo media were higher than those of G1.3/G2.3 serial culture media, but there was no significant difference among the three groups. The total cell number of blastocysts in PZM-3 embryo culture media containing $5{\mu}g/ml$ insulin was significantly higher than that of the control (no insulin) ($44.3{\pm}9.1$ vs. $33.9{\pm}11.7$). For isolation of PESCs-like, the rates of porcine blastocysts attached to feeder layers and ICM colony formation in Method B (nude embryo culture) were better than those in Method A (intact embryo culture).

• Korean Parent-Child Health Journal
• /
• v.5 no.2
• /
• pp.145-160
• /
• 2002

The primary purpose of this study was to suggest midwife education programs which could be recognized and exchanged internationally by examining and analyzing both domestic and foreign midwife education programs. The secondary purpose of this study was to offer a plan to raise the level of national examination. Specific aims of this study were as follows: 1) to identify the international standard of the education and practices of midwives 2) to analyze both domestic and foreign midwife education programs 3) to offer a new curriculum for educating midwives 4) to suggest a prerequisite to raise the standards of the national examination 5) to suggest subjects for the national examination The results of this research were as follows: 1. The concept of midwife and midwifery practices recognized internationally by WHO and ICM(International Confederation of Midwives) was identified. In addition, Core Competencies for Basic Midwifery Practice suggested by ACNM(American College of Nurse-Midwives) of the U.S.A. were examined. 2. Midwife education programs of the U.S.A., Sweden, Australia, and Japan were investigated and analyzed. In addition, the midwife education program stated in the public health related law of this country as well as curriculums of institutions for midwife education were also investigated and analyzed. 3. As for the midwife education system, both a graduate program for midwife education in the college of nursing sciences and a postgraduate professional midwife education program centered medical institutions were suggested. 4. A new curriculum that could promote more international exchanges and extend the role of midwives was suggested after studying both domestic and foreign midwife education programs. 5. A prerequisite to raise the level of national examination for midwives was suggested. In addition, subjects for the examination which could evaluate the applicant's comprehensive thinking ability were presented with its respective range and ratio. A midwife is a medical professional who has a nursing license and is licensed nationally as a midwife with an additional year of education. An effort to extend a midwife' role and to improve its service is imperative. The laws related midwives should be revised in regard to education, service, and the national examination to the level of developed countries so that international recognition can take place. In addition, midwife curriculum and its service should be evaluated periodically. A system must be established to renew midwife licences.

• Journal for History of Mathematics
• /
• v.20 no.3
• /
• pp.127-146
• /
• 2007

From 1876 to 1883, British mathematician James Joseph Sylvester worked as the founding head of Mathematics Department at the Johns Hopkins University which has been known as America's first school of mathematical research. Sylvester established the American Journal of Mathematics, the first sustained mathematics research journal in the United States. It is natural that we think this is the most exciting and important period in American mathematics. But we found out that the International Congress of Mathematicians held at the World's Columbian Exposition in Chicago, August 21-26, 1893 was the real turning point in American's dedication to mathematical research. The University of Chicago was founded in 1890 by the American Baptist Education Society and John D. Rockefeller. The founding head of mathematics department Eliakim Hastings Moore was the one who produced many excellent American mathematics Ph.D's in early stage. Many of Moore's students contributed to build up real American mathematics research power in early 20 century The University also has a well-deserved reputation as the "teacher of teachers". Beginning with Sylvester, we analyze what E.H. Moore had done as a teacher and a head of the new department that produced many mathematical talents such as L.E. Dickson(1896), H. Slaught(1898), O. Veblen(1903), R.L. Moore(1905), G.D. Birkhoff(1907), T.H. Hilderbrants(1910), E.W. Chittenden(1912) who made the history of American mathematics. In this article, we study how Moore's vision, new system and new way of teaching influenced American mathematical society at early stage of the top class mathematical research. and the meaning that early University of Chicago case gave.

• Journal of Korean Society of Rural Planning
• /
• v.17 no.4
• /
• pp.25-38
• /
• 2011

본 연구는 방글라데시의 친환경농업 실태와 농업인들의 인식을 바탕으로 친환경농업 개발에 대한 정부의 역할과 정책적 대안을 모색하고자 하였다. 연구 자료는 방글라데시 탕가일 지역의 농업인을 대상으로 유기농가 100가구와 일반농가 100가구를 무작위로 추출하여 설문조사를 통하여 수집되었다. 인구 과밀의 방글라데시 정부는 식량안보를 위하여 지력 저하 및 환경악화를 방지하고자 많은 노력을 기울이고 있다. 즉 지속 가능한 농업개발을 위하여 통합해충관리(IPM), 통합작물 관리(ICM)를 비롯하여 토양의 비옥도 개선, 페로몬트랩이나 자연 천적을 이용한 해충방제 등 다양한 프로그램을 운영하고 있다. 농업인들의 의견 조사에 따르면 친환경농업에 대한 정부의 역할은 긍정적으로 평가하고 있으나 정책이나 관련 프로그램은 아직 부족한 것으로 나타났다. 일반농가인 경우 극히 소수 농가만이 친환경농업을 실행하고 있는데, 이는 관련기술에 대한 지식의 부족과 유기농 비료 등의 자재 부족이 친환경농업을 실행하는데 가장 큰 어려움이라 지적하고 있다. 일반농가의 39%는 유기농업 의사를 보이고 있지만 마케팅시설의 부족과 농사 실패에 대한 두려움 등을 유기농으로 전환하지 못하는 주요 이유로 들고 있다. 일반농가의 일부(17%)만이 정부의 농업교육 프로그램임인 통합해충관리(IPM)과정을 이수하고 있다. 정부의 유기농 정책은 거의 전무하며 NGO들의 지원에 의하여 꾸준하게 진행되고 있을 뿐이다. 따라서 농업인들은 통합해충관리(IPM), 유기농 퇴비, 페로몬트랩이나 자연 천적 등 매우 다양한 농민교육프로그램들을 정부 주도적으로 실시하여야 하며 친환경농업정책을 보다 명확하게 규정하기를 요구하고 있다. 비교적 젊고 교육수준이 높은 소규모 농업인들이 유기농에 적극적임을 알 수 있다. 유기농의 평균 농지규모는 0.246ha, 유기농 평균 경력은 7.63년, 일반농가대비 농가수입은 11.4% 높게 나타났고 비교적 소농들이 높은 수익률을 보이고 있다. 소비자의 신뢰, 마케팅시설, 생산자단체나 조직의 부족 등이 유기농가들의 가장 큰 애로점이 되고 있고, 유기농산품의 표준화를 위하여 정부의 인증제도와 인증기관 설립이 필요함을 지적하고 있다. 유기농을 보다 활성화하기 위해서는 정부 주도하에 마케팅을 비롯하여 교육, 수출 및 각종 장려책 등이 마련되어야 할 것이다.

• Proceedings of the Korean Society for Bio-Environment Control Conference
• /
• 1998.05a
• /
• pp.153-157
• /
• 1998

무등산 수박의 유묘 생산에 있어 적정 배양액 농도를 구명하기 위하여 일본원시균형배양액을 기준으로 하여 NO$_3$-N, K 및 Ca의 농도별 첨가량에 따른 유묘생장반응을 조사하였다. 1. 배양액내 질소농도을 증가시킬수록 초장, 엽면적, 엽수, 엽장, 엽폭이 증가하였으며 생체중과 건물중도 증가하였다. 2. 배양액내 칼륨의 농도를 증가시켰을 경우 수박 유묘의 초장은 200ppm까지는 약간 증가하지만 엽면적, 엽수, 엽장, 생체중 및 건물중은 200ppm이상으로 증가시키면 감소하는 경향이었다. 3. 칼슘처리의 경우 농도의 증가가 생장의 증가를 나타내지는 않았으며 원시균형 배양액의 표준이라 할 수 있는 80ppm처리구의 생장이 양호한 것으로 나타났다. 4. 배양액(일본원시균형배양액 1/2농도)에 N의 농도를 200ppm으로 증가시킬 경우 수박유묘의 엽병내 N, K 및 Mg의 함량이 증가하였지만 P 및 Ca의 증가나 감소의 경향은 보이지 않았다. 5. K의 농도를 100ppm으로 증가시킬 경우 수박유묘의 엽병내 N, K 및 Mg의 함량이 증가하였지만 P 및 Ca의 증가나 감소의 경향은 보이지 않았다. 5. K의 농도를 150ppm으로 증가시킬 경우 수박유묘의 엽병내 N, K 및 Mg의 함량이 증가하였지만 P 및 Ca의 증가나 감소의 경향은 보이지 않았다. 그러나 200ppm이상으로 증가시킬 경우 N과 Mg의 감소가 나타났다. 6. 양액내 Ca의 농도를 증가시킬수록 엽병내 N, K Ca 및 Mg의 농도가 증가하는 반면 P의 경우 거의 변화가 없었으며,N의 경우는 200ppm 이상의 농도에서 감소하는 경향을 나타내었다. Mg의 경우는 150ppm까지는 함량의 변화가 없었지만 200ppm 이상의 처리구에서 급격한 함량증가를 나타내었다.~21.2ml가 흘러나와 배지의 공극이 일정하지 않아 시간당 통과하는 양이 일정하지 않았다고 생각되었다. $\bigcirc$ 펄라이트 : 합섬A(비스코스+레이온)급액천의 유입은 소(1$\times$60cm)에서 21.8ml, 중(2$\times$60cm) 33.5ml, 대(3$\times$60cm) 43.4ml가 통과되었고 합섬(폴리에스텔)에서는 19.0~30.7ml로서 급액천의 규격에 따라 통과되는 차이가 있었다. 배지가 규격화되어 있어 급액천의 규격별로 일정하게 유입되었으며 급액천의 재질이 유입에 영향을 미친 것으로 사료되었다. (2) 급액관과 베드상과의 높이에 따른 유출양 : 급액과 베드상과의 낙차가 클수록 유출이 증가함을 알수 있었으나 합섬C(인견)실험구에서는 낙차가 유출에 영향을 미치지 않았다. (4) 급액된 양액의 EC 및 pH조사 : 급액된 양액의 EC 및 pH에 전혀 변화가 없어 재배 적응에 문제가 없을것으로 사료되었다.이가 가장 이상적인 것으로 생각된다.세포수에 대한 내부세포괴세포(ICM/total cells)가 20~40% 범주에 드는 비율은 처리구가 대조구보다 낮은 결과를 나타냈다. 결론적으로 돼지난포란을 이용하여 체외성숙을 유기할 때 효과적인 cysteamine의 농도는 50$\mu$M이 적당하며, 초기배발달을 유기할 때의 효과적인 cysteamine의 농도는 25~50$\mu$M인 것으로 판단된다.N)A(N)/N을 제시하였다(A(N)=N에 대한 A값). 위의 실험식을 사용하여 헝가리산 Zempleni 시료(15%$S_{XRD}$)의 기본입자분포로부터 %$S_{XRD}$를 계산한