• Journal of the Institute of Electronics Engineers of Korea SP
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• v.43 no.5 s.311
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• pp.37-44
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• 2006

In this paper, a new version of the ICM method(MICM, modified ICM) in which the contextual information is modelled by Markov random fields (MRF) is introduced. To extract the feature, a new local MRF model with a fitting block neighbourhood is proposed. This model selects contextual information not only from the relative intensity levels but also from the geometrically directional position of neighbouring cliques. Feature extraction depends on each block's contribution to the local variance. They discriminates it into several regions, for example context and background. Boundaries between these regions are also distinctive. The proposed algerian performs segmentation using directional block fitting procedure which confines merging to spatially adjacent elements and generates a partition such that pixels in unified cluster have a homogeneous intensity level. From experiment with ink rubbed copy images(Takbon, 拓本), this method is determined to be quite effective for feature identification. In particular, the new algorithm preserves the details of the images well without over- and under-smoothing problem occurring in general iterated conditional modes (ICM). And also, it may be noted that this method is applicable to the handwriting recognition.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.1
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• pp.62.1-62.1
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• 2018

Galaxies in the cluster environment interact with the intracluster medium (ICM), losing the interstellar medium (ISM) and alternating their evolution. Observational evidences of the extraplanar ISM stripped by the ICM's ram pressure are prevalent in HI imaging studies of cluster galaxies. However, current theoretical understanding of the ram pressure stripping (or ICM-ISM interaction in general) is still limited mainly due to the lack of numerical resolution at ISM scales in large-scale simulations. Especially, self-consistent modeling of the turbulent, multiphase ISM is critical to understand star formation in galaxies interacting with the ICM. To achieve this goal, we utilize the TIGRESS simulation suite, simulating a local patch of galactic disks with high resolution to resolve key physical processes in the ISM, including cooling/heating, self-gravity, MHD, star formation, and supernova feedback. We then expose the ISM disk to ICM flows and investigate the evolution of star formation rate and the properties of the ISM. By exploring ICM parameter space, we discuss an implication of the simple ram pressure stripping condition (so called the Gunn-Gott condition) to the realistic ISM.

• Korean Journal of Animal Reproduction
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• v.20 no.1
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• pp.27-34
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• 1996

The objective of this study was to examine the cell number of Total, ICM and TE cells of bovine blastocysts according to development progression cultured in CR1 medium, which was reported as successfully supporting medium for preimplantaion bovine embryo development to the blastocyst stage, by differential labelling of the nuclei with immunosurgery and polynucleot-ide-specific fluorochromes. Blastocysts were obtained at day 8 after in vitro fertilization and classified to early, middle, expanded stage according to the developmental morphology; blastocoel expansion and zona thickness. Also, bias tocysts in the same category were divided into two parts to check the Total cell number by using bisbenzimide only and ICM, TE and Total cell number by using immunosurgery and two polynucleotide-specific fluorochromes. 1) The development rate of blastocysts at day 8 after in vitro fertilization was 29.3% and classified bIas tocysts to early, middle, expanded and hatching stage were 8.7, 9.9, 7.6 and 3.1%, respectively. 2) The numbers of total blastomere using bisbenzimide in the classified blastocysts to early, middie and expanded were 46.9${\pm}$8.6, 66.2${\pm}$12.5 and 122.8 ${\pm}$ 14.4, respectively. This indicated that CR1 is a appropriate culture medium for bovine embryo development. 3) The count of ICM and TE cell number by using differential labelling with immunosurgery and polynucleotide-specific fluorochromes in the classified blastocysts to early, middle and expanded; ICM cell numbers of were 12.8${\pm}$5.9, 26.3${\pm}$8.4 and 35.5${\pm}$15.0, respectively and TE cell numbers were 30.5${\pm}$5.0, 4 41.3${\pm}$8.2 and 81.1${\pm}$13.4, respectively. These results presented that the increase of ICM and TE cell numbers averaged two and three doublings between early and expanded blastocyst stage and also total cell number counted from ICM nuclei and TE nuclei by using differential label-ling showed the increase pattern with development advance level and the results were similar to total cell number obtained from bisbenzimide treatment only. Therefore, the differential labelling of ICM and TE nuclei in situ is a very useful technique to evaluate embryo qualities and can be used as an indicator on study of preim-plantation embryo development.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.1
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• pp.43-49
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• 1997

The objective of this study is to determine developmental pattern and cell allocation to the ICM and TE in haploid and diploid of embryos following parthenogenetic activation and in vitro fertilization. The incidence of development to blastocyst was lower in the combined treatments of ethanol stimulation and cytochalasin B as compared to the control. However, the combined ethanol stimulation and cytochalasin B treatment (diploid) accelerated development to the blastocyst as compared to the ethanol treatment alone (haploid). Significantly reduction in the average number of total cells and ICM was observed in the parthenotes alone as compared to fetilized embryos, but those of combined ethanol stimulation and cytochalasin B treatment embryos were significantly increased as compared to ethanol alone embryos. These results suggested that the ploidy affects preimplantation developmental patters and cell allocation to the ICM and TE in the porcine.

• Reproductive and Developmental Biology
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• v.28 no.4
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• pp.227-233
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• 2004

This study was conducted to investigate the effect of various addition and exclusion time of glucose (Control: no addition, A: 24～72 h, B: 24～48 h, C: 48～72 h, D: 0～72 h, E: 0～48 h, F: 0～24 h and 48～72 h, G: 0～24 h) on embryonic developmental capacity of 2-cell embryos in mice. Developed blastocysts were assessed for mean cell number by differential staining. The zona-intact blastocyst (ZiB) rates were higher (p<0.05) in group B than control. However, the zona-escape blastocyst (ZeB) rates were not significantly different in all groups. At 72 h, total blastocyst (ZiB + ZeB) formation rates were not significantly different in all groups. The mean cell number was not significantly different among all groups. The inner cell mass (ICM) cell number was higher (p<0.05) in group F than control, group A, B and G. The trophectoderm (TE) cell number was higher (p<0.05) in control than group A and D. The %ICM was higher (p<0.05) in group C, D and F than control. The ICM : TE ratio was not significantly different in all groups. Between control and glucose group, no significant difference was observed in the total blastocysts (ZiB + ZeB) formation rates. Also, no significant difference was observed in the mean cell number, ICM cell number and ICM : TE ratio. However the TE cell number was higher (p<0.05) in control than glucose group and %ICM was higher (p<0.05) in glucose group than control. In conclusion, glucose added in culture medium was not inhibitory on blastocyst formation but glucose added for 48 ～72 h in culture medium increases %ICM of blastocysts in mice.

• The KIPS Transactions:PartB
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• v.13B no.5 s.108
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• pp.525-532
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• 2006

This paper proposes a new class of image restoration on the Ising modeled binary 'Takbon' image by the flexible line-fitting ICM(Iterated conditional modes) method. Basically 'Takbon' image need be divided into two extreme regions, information and background one due to its stroke combinations. The main idea is the line process, comparing with the conventional ICM approaches which were based on partially rectangular structured point process. For calculating geometrical mechanism, we have defined line-fitting functions at each current pixel array which form the set of linear lines with gradients and lengths. By applying the Bayes' decision to this set, the region of the current pixel is decided as one of the binary levels. In this case, their statistical reiteration for distinct tracking between intra and extra region offers a criterion to decide the attachment at each step. Finally simulations using the binary 'Takbon' image are provided to demonstrate the effectiveness of our new algorithm

• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.193-198
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• 2007

The present study was examined the expression patterns of cdx2 gone, n lineage marker, in the mouse and bovine developmental stage embryos and whether one blastomere of two- and/or four-cell bovine embryos develop to specific lineage (ICM or TE) of blastocyst by injection of Texas red conjugated dextran as a lineage tracer. It was also investigated the allocation of ICM and n cells in bovine blastocysts derived from one blastomere of two-and/or four-cell stage embryos. Firstly, it was observed that expression of cdx2 appeared symmetric and asymmetric distribution at the two-cell stage mouse embryos. from four-cell to morula stage mouse embryos, the expression of cdx2 gene was observed in almost all blastomeres. In case of bovine embryos, localization of cdx2 was similar to pattern of mouse embryos. The Dextran-labeled blastomere of two- and/or four-cell embryos contributed to both ICM and TE cells in bovine blastocysts. And also, it was confirmed that a single blastomere derived from two-cell stage bovine embryos could develop to the normal blastocyst with both ICM and TE cells. These results show that two-and/or four-cell stage is not the specific stage to determine the cell rate for ICM and TE, and which is not correlated with the expression of cdx2 gene.

• Clinical and Experimental Reproductive Medicine
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• v.29 no.4
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• pp.237-243
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• 2002

Objective : The aim of this study was to evaluate the influence of different media on blastulation, mean cell number, percentage of inner cell mass (ICM) of total cells and ICM : trophectoderm (TE) ratio in mice. Materials and methods: A total 552 two cell embryos were retrieved from ICR female mice (4 weeks old) at 48 hr after hCG injection (mated just after hCG injection) and cultured in MEM (n=276) or TCM (n=276) supplemented with 20% hFF. The grading of blastocysts from zona-intact (ZiB) to -escape (hatching and hatched, ZeB) was performed at 72 hours after culture. Total, TE and ICM cell numbers were analyzed by differential staining of blastocyst. Statistical analysis was performed using t-test with SigmaPlot-2001. P-values < 0.05 were accepted as statistically significant. Results: The blastulation rate in MEM ($64.9{\pm}4.95%$) was significantly higher (p=0.0031) than that in TCM ($57.2{\pm}5.22%$). No differences were found in the number of ZiB and ZeB between MEM ($31.9{\pm}2.62$, $33.0{\pm}4.58%$), and TCM ($27.2{\pm}4.28$, $30.1{\pm}4.58%$). A total 314 blastocysts (MEM=166; TCM=148) were stained differentially. Mean cell number of blastocysts was significantly higher (p=0.0002) in TCM ($73.1{\pm}3.3$) than in MEM ($61.7{\pm}2.5$). Differential staining was successfully performed in 155 blastocysts (MEM=77; TCM=78). The percentage of ICM was significantly higher in MEM than in TCM ($20.9{\pm}1.3$ vs. $17.1{\pm}1.2%$, p=0.0281). The ICM : TE ratio was higher in TCM than in MEM (1 : $4.85{\pm}0.68$ vs. 1 : $3.78{\pm}0.78$, NS). Conclusion: These results show that MEM increase the blastocyst formation and percentage of ICM of total cells comparing with TCM in mice.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.49.2-49.2
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• 2015

IntraCluster Medium (ICM) located at the galaxy cluster is in the state of very hot, tenuous, magnetized, and highly ionized X-ray emitting plasmas. High temperature and low density make ICM very viscous and conductive. In addition to the high conductivity, fluctuating random plasma motions in ICM, occurring at all evolution stages, generate and amplify the magnetic fields in such viscous ionized gas. The amplified magnetic fields in reverse drive and constrain the plasma motions beyond the viscous scale through the magnetic tension. Moreover, without the influence of resistivity viscous damping effect gets balanced only with the magnetic tension in the extended viscous scale leading to peculiar ICM energy spectra. This overall collisionless magnetohydrodynamic (MHD) turbulence in ICM was simulated using a hyper diffusivity method. The results show the plasma motions and frozen magnetic fields have power law of $E_V^k{\sim}k^{-3}$, $E_M^k{\sim}k^{-1}$. To explain these abnormal power spectra we set up two simultaneous differential equations for the kinetic and magnetic energy using an Eddy Damped Quasi Normal Markovianized (EDQNM) approximation. The solutions and dimensions of leading terms in the coupled equations derive the power spectra and tell us how the spectra are formed. We also derived the same results with a more intuitive balance relation and stationary energy transport rate.

• Clinical and Experimental Reproductive Medicine
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• v.43 no.3
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• pp.152-156
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• 2016

Objective: We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. Methods: Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona opening performed at the site of the ICM, n=125), the trophectoderm (TE) group (zona opening performed opposite to the ICM, n=125) and the control group (no zona opening, n=125). Results: The rate of complete hatching of the blastocysts was not significantly different in the ICM and the TE group (84.8% vs 80.8%, respectively; p=0.402), but was significantly lower in the control group (51.2%, p<0.001). The cell numbers in the completely hatched blastocysts were comparable in the control group, the ICM group, and the TE group ($69{\pm}19.3$, $74{\pm}15.7$, and $71{\pm}16.8$, respectively; p=0.680). Conclusion: These findings indicate that the site of laser zona opening did not influence the rate of complete hatching of mouse blastocysts or their cell numbers.