• 미생물학회지
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• 제46권3호
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• pp.255-261
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• 2010

남태평양에 서식하는 두 종의 해면 Hyrtios sp. 604와 Callyspongia sp. 612로부터 16S rDNA DGGE 방법을 이용하여 공생세균 군집의 다양성을 분석하였다. DGGE band로부터 염기서열 분석 결과, Hytrios sp. 604의 공생세균은 Alphaproteobacteria, Gammaproteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, Cyanobacteria, Firmicutes로 나타났으며, Callyspongia sp. 612의 공생세균 그룹은 Alphaproteobacteria, Gammaproteobacteria, Chloroflexi, Cyanobacteria로 나타났다. 풍부한 이차대사산물의 생산자로 보고된 Hyrtios 해면 속의 Hyrtios sp. 604는 Callyspongia sp. 612에 비해 더 다양한 공생세균 군집을 나타내었으며 주요 공생세균 군집으로 Actionobacteria가 포함되었다. 동일 지역에 서식하나 화학적 특성이 다른 두 해면 종의 세균 군집은 서로 다른 것으로 나타났다. 발견된 공생세균의 염기서열은 90% 이상이 uncultured clone들과 높은 상동성을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.2006-2011
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• 2016

The marine sponges Hyrtios and Haliclona species, both of which are known to produce secondary bioactive metabolites, were used to extract 1304KO-327 and 1304KO-328. Such secondary metabolites are potentially antibacterial, antiviral, anti-inflammatory, antitumoral, antifungal, and antiplasmodial. In the present study, the effects of 1304KO-327 and 1304KO-328 were studied for their clinical and pathological importance. The cytotoxicity of 1304KO-327 and 1304KO-328 was assessed via MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay on HT-29, Caco-2, and Raw 264.7 cells. Rotavirus-infected Caco-2 cells were used to prove the antiviral effects of the marine sponge extracts. The test results cogently proved that the virus-inhibiting effects of the sponge extracts improved with extract concentration. Anti-inflammatory effects of the marine sponge extracts were tested on Lipopolysaccharide-treated Raw 264.7 cells. Nitric oxide and cytokine were produced by treatment of the cells with LPS and the inhibiting effects of the sponge extracts on $IL-1{\beta}$ formation were investigated. This study found that the NO production was decreased dose dependently, and $IL-1{\beta}$ formation was significantly reduced by the marine sponge extracts.