• 한국패류학회지
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• 제29권3호
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• pp.181-187
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• 2013

In order to investigate environmental stress inducible genes in abalone, we analyzed differentially expressed transcripts from a Pacific abalone, Haliotis discus hannai, after exposure to heat-, cold- or hyposalinity-shock by suppression subtractive hybridization (SSH) method. 1,074 unique sequences from SSH libraries were composed to 115 clusters and 986 singletons, the overall redundancy of the library was 16.3%. From the BLAST search, of the 1,316 ESTs, 998 ESTs (75.8%) were identified as known genes, but 318 clones (24.2%) did not match to any previously described genes. From the comparison results of ESTs pattern of three SSH cDNA libraries, the most abundant EST was different in each SSH library: small heat shock protein p26 (sHSP26) in heat-shock, trypsinogen 2 in cold-shock, and actin in hyposalinity SSH cDNA library. Based on sequence similarities, several response-to-stress genes such as heat shock proteins (HSPs) were identified commonly from the abalone SSH libraries. HSP70 gene was induced by environmental stress regardless of temperature-shock or salinity-stress, while the increase of sHSP26 mRNA expression was not detected in cold-shock but in heat-shock condition. These results suggest that the suppression subtractive hybridization method is an efficient way to isolate differentially expressed gene from the invertebrate environmental stress-response transcriptome.

• 한국양식학회지
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• 제15권1호
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• pp.69-75
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• 2002

사육수의 저염분에 따른 급성 스트레스 반응 (코티졸, 글루코스, Na$^{+}$, K$^{+}$, Cl￣, 총단백질, ALT, AST, 헤마토크리트, 적혈구수, 헤모글로빈 농도, MCV, MCH 및 MCHC) 조사하였다. 예비사육 수조에서 사육하던 어류를 해수(35$\textperthousand$)가 공급되는 실험수조에서 30분이내에 담수 (0 $\textperthousand$)를 흘려주면서 l5 $\textperthousand$구와 0 $\textperthousand$구로 사육수를 교환하였다. 실험개시시 코티졸 농도는 1.6${\pm}$0.2 ng/$m\ell$로부터 15 $\textperthousand$구는 1시간째 3.5${\pm}$1.8 ng/$m\ell$, 3시간째와 24시간째에는 각각 1.7${\pm}$0.2 ng/$m\ell$와 3.0${\pm}$2.4 ng/$m\ell$로 유의한 차이를 보이지 않았다. 그러나 0 $\textperthousand$구는 1시간째 22.8${\pm}$3.8 ng/$m\ell$, 3시간째 9.3${\pm}$1.8 ng/$m\ell$, 24시간째 9.5${\pm}$0.2 ng/$m\ell$로 높아진 값을 보였다. 글루코스 함량은 실험개시시 17.7${\pm}$0.6 mg/dl로부터 15$\textperthousand$구와 0$\textperthousand$구는 1시간째 각각 96.2${\pm}$1.6 mg/dl, 155.8${\pm}$16.0 mg/dl로 높아졌다. 그러나 24시간째에는 각각 13.0${\pm}$3.2 mg/dl, 15.2${\pm}$1.4 mg/dl로 실험개시시 값으로 회복되었다. Na$^{+}$과 K$^{+}$은 15 $\textperthousand$구에서 차이를 보이지 않았으나, 0 $\textperthousand$구에서는 감소하는 경향을 보였다. 생존율은 48시간째부터 폐사하기 시작하여 144시간째에는 전량폐사하였다. 삼투압 조절 능력을 위한 여러가지 파라메타에서 15 $\textperthousand$구는 이상이 없는 것으로 추측되나, 0 $\textperthousand$구에서는 코티졸, Na$^{+}$, K$^{+}$, Cl￣, 총단백질 및 AST에서 시간경과에 따른 삼투압 조절 능력에 문제가 있는 것으로 보여진다.