• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.167-167
• /
• 2017

Our previous study for developing seeds of Iksan 526 (I.526), an inbred line of resveratrol-producing transgenic rice line, showed that, in 20 days after heading (DAH) seeds, resveratrol was almost saturated and accumulation of piceid was highest though the expression of Arachis hypogaea resveratrol synthase 3 (AhRS3, GenBank DQ124938) was highest in 31 DAH seeds. In this study, it was investigated how the overexpression of AhRS3 affects phenylpropanoid pathway genes. p-Coumaroyl-CoA is derived from phenylpropanoid pathway and used as a substrate of AhRS3 reaction for resveratrol production. In 6, 13, 20, 31 and 41 (45 for Dongjin) DAH seeds of I526 and Dongjin, a wild type of I.526, respectively, the expression pattern of phenylpropanoid pathway genes, including phenylalanine ammonia-lyase (PAL: LOC_Os02g41630.2, LOC_Os04g43760.1), cinnamate 4-hydroxylase (C4H: LOC_Os05g25640.1), 4-coumarate-CoA ligase (4CL: LOC_Os02g08100.1), cinnamoyl-CoA reductase (CCR: LOC_ Os09g25150.1, LOC_Os08g34280.1), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT: LOC_Os04g42250.2, LOC_Os02g39850.1) and cinnamyl alcohol dehydrogenase (CAD: LOC_Os02g09490.1), was examined using real time (RT)-PCR. Compared to developing seeds of Dongjin, RT-PCR results showed that the expression pattern of phenylpropanoid pathway genes was modified in developing seeds of I.526. In most genes, except for CAD, of I.526 developing seeds, the gene expression was highest in 20 DAH corresponding to biosynthesis of resveratrol and piceid, i.e. the expression of phenylpropanoid pathway genes was gradually increased by 20 DAH and decreased as seeds develop. Especially, in Dongjin, the highest expression of PALs and 4CL was in 6 DAH and their expression was gradually decreased as seeds develop. These genes expression data also exhibited that, in developing seeds of I.526, phenylpropanoid pathway genes were slightly or significantly (in some genes) upregulated compared to Dongjin. Therefore, the overexpression of AhRS3 changed the expression pattern of phenylpropanoid pathway genes in I.526 developing seeds and this modification for gene expression is closely related to biosynthesis of resveratrol and piceid.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.105-105
• /
• 2017

Our previous study for developing seeds of Iksan 526 (I.526), an inbred line of resveratrol-producing transgenic rice line, showed that, in 20 days after heading (DAH) seeds, resveratrol was almost saturated and accumulation of piceid was highest though the expression of Arachis hypogaea resveratrol synthase 3 (AhRS3, GenBank DQ124938) was highest in 31 DAH seeds. In this study, it was investigated how the overexpression of AhRS3 affects phenylpropanoid pathway genes. p-Coumaroyl-CoA is derived from phenylpropanoid pathway and used as a substrate of AhRS3 reaction for resveratrol production. In 6, 13, 20, 31 and 41 (45 for Dongjin) DAH seeds of I526 and Dongjin, a wild type of I.526, respectively, the expression pattern of phenylpropanoid pathway genes, including phenylalanine ammonia-lyase (PAL: LOC_Os02g41630.2, LOC_Os04g43760.1), cinnamate 4-hydroxylase (C4H: LOC_Os05g25640.1), 4-coumarate-CoA ligase (4CL: LOC_Os02g08100.1), cinnamoyl-CoA reductase (CCR: LOC_Os09g25150.1, LOC_Os08g34280.1), hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyl transferase (HCT: LOC_Os04g42250.2, LOC_Os02g39850.1) and cinnamyl alcohol dehydrogenase (CAD: LOC_Os02g09490.1), was examined using real time (RT)-PCR. Compared to developing seeds of Dongjin, RT-PCR results showed that the expression pattern of phenylpropanoid pathway genes was modified in developing seeds of I.526. In most genes, except for CAD, of I.526 developing seeds, the gene expression was highest in 20 DAH corresponding to biosynthesis of resveratrol and piceid, i.e. the expression of phenylpropanoid pathway genes was gradually increased by 20 DAH and decreased as seeds develop. Especially, in Dongjin, the highest expression of PALs and 4CL was in 6 DAH and their expression was gradually decreased as seeds develop. These genes expression data also exhibited that, in developing seeds of I.526, phenylpropanoid pathway genes were slightly or significantly (in some genes) upregulated compared to Dongjin. Therefore, the overexpression of AhRS3 changed the expression pattern of phenylpropanoid pathway genes in I.526 developing seeds and this modification for gene expression is closely related to biosynthesis of resveratrol and piceid.

• Korean Journal of Breeding Science
• /
• v.41 no.4
• /
• pp.635-639
• /
• 2009

A new peanut variety "Pungan"(Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Functional Crop, NICS(National Institute of Crop Science), in Milyang in 2008. It was developed from the cross between the high-yielding cultivar "Saedl" and the very short stem cultivar "Satonoka". "Pungan", a Virginia palnt type, has 20 branches with long ellipseshaped large grains. The 100 grain weight was 88 g, 7 g heavier than the check variety Daekwang. This variety is more resistant to late leaf spot and web blotch compared to Daekwang, a check variety. Moreover, it is lodging resistant owing to its short stem. "Pungsan" outyielded the check variety by 16% with 4.67 MT/ha in regional yield trials.

• Korean Journal of Breeding Science
• /
• v.40 no.2
• /
• pp.173-177
• /
• 2008

A new peanut variety "Pungsan" (Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2007. It was derived from a cross between a short stem cultivar "Satonoka" and a high-yielding local cultivar "Yecheon". "Pungsan" is the Virginia plant type. It has 18 branches per plant and 40 cm of main stem height. Each pod with long-ellipse shaped large kernel has two grains with light-brown testa and 100 seed weight was 95g in the regional yield trials (RYT). "Pungsan" showed more resistant to web blotch compared with check variety. In the regional yield trials "Pungsan" was outyielded in grain yield to the check variety by 16% with 4.45 MT/ha for grain.

• Korean Journal of Breeding Science
• /
• v.40 no.1
• /
• pp.63-67
• /
• 2008

A new peanut cultivar "Jopyeong" (Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2006. It was developed from the cross between the very short stem cultivar "CUP brittle" and the high-yielding cultivar "Daekwang". "Jopyeong" which is Virginia plant type has 23 branch number per plant with early maturing and ellipse-shaped large kernel. Each pod has two grains with brown testa and 100 seed weight was 87g in the regional yield trials (RYT). This variety also showed more resistant to late leaf spots compared with check one. Especially it has resistance to lodging owing to short stem and erect plant type. In the regional yield trials "Jopyeong" was out-yielded than check variety by 11% with 8.37 ton/ha for fresh pod and by 4% with 3.95 ton/ha for grain.

• Korean Journal of Breeding Science
• /
• v.40 no.2
• /
• pp.178-183
• /
• 2008

A new peanut variety "Seonan" (Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2007. It was developed from the cross between the erect short stem cultivar "SP9128" and the high-yielding cultivar "Daekwang". "Seanan" which is Virginia plant type has 18 branch number per plant with early maturing and long-ellipse shaped large kernel. Each pod has two grains with brown testa and 100 seed weight was 87 g in the regional yield trials (RYT). Especially this variety has resistance to lodging owing to short stem. The free sugar and tannin content of fresh peanut are 3.7% and 0.48%, respectively, similar to check variety. In the regional yield trials "Seonan" was outyielded than check variety by 14% with 8.59 MT/ha for fresh pod and by 7% with 3.75 MT/ha for grain.

• Korean Journal of Breeding Science
• /
• v.40 no.2
• /
• pp.168-172
• /
• 2008

A new peanut variety "Charmpyeong" (Arachis hypogaea ssp. hypogaea L.) was developed at the Yeongnam Agricultural Research Institute, NICS, in Milyang in 2007. It was developed from the cross between the high-yielding cultivar "Shindaekwang" and the very short stem cultivar "ICGV94216". This Virginia plant typed "Charmpyeong" has 33 cm stem height and 18 branch number Each pod with ellipse-shaped large kernel has two grains with brown testa and 100 seed weight was 88 g in the regional yield trials (RYT). This variety also showed more resistant to web blotch compared with check one. Especially it has resistance to lodging until harvesting owing to short stem. In the regional yield trials "Charmpyeong" was outyielded than check variety by 13% with 4.35 MT/ha for grain.

• Korean Journal of Breeding Science
• /
• v.49 no.3
• /
• pp.224-229
• /
• 2017

A new peanut variety 'Daan'(Arachis hypogaea ssp. hypogaea L.) was developed at the Department of Southern Area Crop Science, National Institute of Crop Science (NICS) in Milyang 2014. This was developed from the cross between 'Iksan 31' with Virginia typed short stem and 'Dakwang' with Shinpung-typed larger grain. 'Daan' which is a Shinpung plant type had 13 branches per plant and its length of main stem was 44cm. Each pod has two grains with brown testa and long ellipse-shaped large kernel. Its yield components showed 34 pods per plant, 127 g of 100-seed-weight and 75% of pod shelling ratio in the regional yield trials (RYT). Seed quality showed 47.8% of crude oil and 28.3% of protein content. This variety showed resistant to early leaf spot and had more resistant to late leaf spot, stem rot and lodging, compared to reference variety. In the regional yield trials for 3 years 'Daan' was more productive than reference variety by 16% with 5.00 MT/ha for grain production.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 1986.06a
• /
• pp.90-91
• /
• 1986

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2003.10a
• /
• pp.170-170
• /
• 2003