• International Journal of Industrial Entomology and Biomaterials
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• 제22권2호
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• pp.75-82
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• 2011

To elucidate the novelty of Hyphantria cunea nucleopolyhedrovirus (HcNPV) isolated in Korea, polyhedrin and inhibitor of apoptosis (iap) gene structures were determined and analyzed. The analysis of HcNPV polyhedrin showed a little difference with 97.6% at the nucleotide level but no difference at the amino acid level when compared with that of previously reported H. cunea NPV (HycuNPV). On the other hand, iap genes showed variable differences with 89.0-99.6% nucleotide and 90.0-99.6% amino acid sequence identities. Especially, the 5' and 3' non-coding flanking sequences of iap1 gene had lower degree of identity with those of HycuNPV. Although the phylogenetic analyses using polyhedrin and iap genes showed that HcNPV is closely related with HycuNPV, we could provide that HcNPV is a novel isolate having novel gene structures.

• 한국유기농업학회지
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• 제31권4호
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• pp.395-412
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• 2023

광식성 난방제 해충인 미국흰불나방(Hyphantria cunea)의 친환경 방제를 위한 바이러스 살충제의 소재 활용을 위해서 국내에서 분리된 미국흰불나방 핵다각체병바이러스(H. cunea nucleopolyhedrovirus W1: HycuNPV-W1)의 다각체 형태 및 전장 유전체 서열을 결정하고 분석하였다. HycuNPV-W1의 다각체는 1.5-2.2 um 크기의 사면체에 가까운 부정형으로 확인되었다. 전장 유전체의 염기서열을 결정한 결과, 기존에 보고된 HycuNPV와 비교할 때 1,606 bp 더 짧은 131,353 bp로 확인되었다. 그러나 G+C 함량은 45%였으며 상동반복영역은 6개로 기존에 보고된 HycuNPV와 차이는 확인할 수 없었다. ORF 분석에서는 HycuNPV-W1은 기존의 HycuNPV와 비교할 때 3개의 ORF가 더 적은 145개를 가졌으나, HycuNPV-W1에만 존재하는 2개의 ORF가 확인되었다. 이들 ORF의 기능은 현재까지 밝혀지지 않았으나 바이러스의 생물학적 특성에 큰 영향을 주지 않을 것으로 추정되었다. 유전체의 vista 분석 결과에서는 HycuNPV-W1과 기존 HycuNPV의 전체 염기서열 유사도가 매우 높은 것으로 확인되었다. 국내에서 처음으로 분석한 HycuNPV-W1의 전장 유전체는 기존의 HycuNPV와 매우 유사한 것으로 나타났으나, 독자적인 특성을 가진 서로 다른 분리주로 국내 고유자원임을 확인할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1485-1490
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• 2006

In this study, comparative replicational properties of Hyphantria cunea nucleopolyhedrovirus (HycuNPV) in Lymantria dispar (IPLB-LdElta) and Spodoptera frugiperda (IPLB-Sf21) cell lines were investigated. Our microscopic observations showed that cytopathic effects (CPEs) in LdElta cells appeared 12 h later than those in Sf21 cells. Whereas polyhedral inclusion bodies (PIBs) formed at 48 h postinfection (p.i.) in LdElta cells, it formed at 36 h p.i. in Sf21 cells. Extracellular virus production determined according to the 50% tissue culture infective dose ($TCID_{50}$) method in LdElta cells started about 12 h later when compared with Sf21 cells. Titers of extracellular virus in LdElta and Sf21 cells were calculated as $1.77{\times}10^9$ plaque forming units (PFU)/ml and $5.6{\times}10^9PFU/ml$, respectively, at 72 h p.i. We also showed that viral DNA replication began at 12 h p.i. in both cell lines. Viral protein synthesis was determined by SDS-polyacrylamide gel electrophoresis (PAGE) and polyhedrin synthesis was observed at 12 h p.i. in both cell lines. The results indicate that while the synthesis of macromolecules is 12 h later and production of extracellular virus is almost 3-fold lower in LdElta cells compared with those in Sf21 cells, the LdElta cell line is still a productive cell line for infection of HycuNPV.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2002년도 Join Meetings of Korean Society of Sericultural Science and Japanse Society of Sericultural Science
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• pp.119-119
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• 2002

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