• 한국수산과학회지
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• 제40권4호
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• pp.187-192
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• 2007

To prepare oligouronic acids from high-molecular-weight alginates prepared from sea tangle (Laminaria japonica), the alginates were hydrolyzed at various temperatures for 1 hr at pH 5.0. The effects of hydrolysis temperature $(H_{Temp})$ on the average molecular weight (AMW) and distribution ratio of MW (DR) in the hydrolyzed alginates were investigated. As $(H_{Temp})$ increased, the AMW of the alginates decreased exponentially; in addition the DR of the alginates with MW>500 kDa decreased exponentially, while the DR of those with MW=50-100 and MW<50 kDa increased exponentially. For the alginates with MW=300-500 and MW=100-300 kDa the DR increased exponentially until $H_{Temp}$ reached $80^{\circ}C$, and then decreased exponentially at above $80^{\circ}C$. AS $H_{Temp}$ increased, the MW cutoff size and AMW Of the alginates fraction With the highest DR both decreased. For $H_{Temp}<60^{\circ}C$, the MW cutoff size with the highest DR was MW>500 kDa; the DR was 39-67% and the AMW 1,000-1,300 kDa. For $H_{Temp}$ $80^{\circ}C$, the MW cutoff size with the highest DR was MW=300-500 kDa and the DR was about 33% and the AMW about 400 kDa. For $H_{Temp}\;100-121^{\circ}C$, the MW cutoff size with the highest DRs was MW=50-100 kDa, with a DR of 39-44% and an AMW of 70-80 kDa.

• Applied Biological Chemistry
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• 제18권3호
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• pp.177-182
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• 1975

돼지감자를 분석(分析)하여 탄수화합물(炭水化合物)로써 존재(存在)하고있는 Inulin성분량(量)이 12.09% 함유(含有)하고 있음을 확인(確認)하였다. pencillium sp 1을 돼지감자배지(培地)에 배양(培養)하여 과당생산조건(果糖生産條件)을 검토(檢討)한 결과(結果)는 다음과 같다. 1. 돼지감자에 대(對)한 최적가수량(最適加水量)은 신선돼지감자 1kg에 대(對)하여 증류수(蒸溜水) 2.5$\ell$이다. 이때의 Inulin 농도(濃度)는 4%(W/V)이였다. 2. opt. temp는 $30^{\circ}C$, opt pH는 Initial pH로 5.0, opt cultcoral period 는 72 hrs 이였다. 3. 배양방법(培養方法)은 500ml shaking flask 에 배지량(培地量)을 50ml로 하고 120oseills/min 으로 shaking culture 가 가장 효과적(?果的)이었다. 4. 질소원(窒素源)으로써는 $NH_4H_2PO_40.1%$ 금속염(金屬鹽)으로써는 $FeSO_47H_2O$ 0.001%, $MgSO_47H_2O$ 0.001%가 가장 효과적(效果的)이었다. 5. 최적배양조건하(最適培養條件下)에서 과당생성(果糖生成)은 72 hrs 까지 계속증가(增加)되었고 최고(最高)의 생산량(生産量)은 95.25%이었다.