• Title/Summary/Keyword: Hydrolysis degree

Search Result 280, Processing Time 0.024 seconds

Preparation and Characterization of the Hydrolyzed Protein from Shaving Scraps of Leather Waste Containing Chromium by the Combination Treatment with Alkaline Inducing Agent and Alkaline Proteolytic Enzyme (Alkaline Inducing Agent 및 Alkaline Proteolytic Enzyme 혼용처리에 의한 Shaving Scraps 가수분해 단백질의 제조 및 특성)

  • Kim, Won-Ju;Cho, Ju-Sik;Lee, Hong-Jae;Heo, Jong-Soo
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.6 no.1
    • /
    • pp.1-12
    • /
    • 1998
  • To examine the possibility of protein recycling of shaving scraps containing chromium generated from manufacturing process of leather, the optimum hydrolysis conditions and the withdrawal methods of low molecular weight protein for using the liquid fertilizer sources by investigation of solubilities of hydrolyzed protein, inorganic nutrients contents and molecular weight distributions of hydrolyzed protein from shaving scraps treated with mixed alkaline inducing agents and mixed alkaline proteolytic enzymes including MgO were investigated. In hydrolysis of shaving scraps treated with mixed alkaline inducing agents, the solubility of shaving scraps were clearly different with 65~85% according to the sorts of the inducing agents, and the degree of hydrolysis was high in the order of NaOH, $Ca(OH)_2$ and KOH. The average molecular weights of withdrawal hydrolyzed protein were 10, 40 and 80 KD treated with NaOH, $Ca(OH)_2$ and KOH, respectively. And the chromium contents was about 15 ppm. In hydrolysis of shaving scraps treated with mixed alkaline proteolytic enzymes, the bility of shaving scraps were high in the order of alcalase, esperase and savinase. In c of treating 0.5% alcalase, the low molecular weight of hydrolyzed protein could be withdrawn. The solubility of the hydrolyzed protein was about 85%, the average molecular weight of the protein was below 1 KD and chrome content of the protein was below 10 ppm.

  • PDF

Recovery of Protein Hydrolysate from Hoki (Johnius belengeri) Frame with Tuna Pyloric Caeca Crude Enzyme and Its Functionalities (참치 유래 조효소를 이용한 민태(Johnius belengeri) Frame으로부터 단백질 가수분해물의 회수 및 그 기능성)

  • Jeon, You-Jin;Lee, Byoung-Jo;Byun, Hee-Guk;Kim, Jong-Bae;Kim, Se-Kwon
    • Applied Biological Chemistry
    • /
    • v.42 no.1
    • /
    • pp.49-57
    • /
    • 1999
  • Enzymatic hydrolysis with tuna pyloric caeca crude enzyme(TPCCE) was performed to recover a protein hydrolysate from hoki frame, fish processing by-product. Optimum hydrolytic conditions were pH 10.0, temperature $50^{\circ}C$, and incubation time 12 hrs, and then the degree of hydrolysis was about 60%. The yield of the hydrolysate from hoki frame by enzymatic hydrolysis was approximately 77% on a dry weight basis. The prepared protein hydrolysates were also fractionated through a series of 30, 10, 5 and 1 kDa molecular weight cut-off (MWCO) membranes in order to investigate the effect of their functionalities according to the difference of their molecular size. As the result of studying functionalities of the hydrolysates, 1 K hydrolysate showed the highest solubility over all pHs, and 30 and 10 K hydrolysate showed more excellent emulsifying property and whippability than the other hydrolysates.

  • PDF

Mechanical and Surface Properties for Akaline Hydrolyzed Polyester Fabrics (알칼리 감량가공 된 폴리에스테르 직물의 역학적 특성과 표면특성에 관한 연구)

  • Kim, Kyung-Ae
    • Journal of the Korean Home Economics Association
    • /
    • v.36 no.8
    • /
    • pp.51-61
    • /
    • 1998
  • This paper discussed the assessment of hand of polyester fabrics changed by alkaline hydrolysis. This study analyzed the relations between the change of mechanical property and the hand value according to the weight loss of polyester fabrics. The results are as follows; Objective hand by KES-FB system, revealed that for tensile properties, as the weight loss increased, WT, RT increased and LT decreased. For bending properties, as the weight loss increased, B and 2HB showed smaller values. For shear properties, as the weight loss increased, G, 2HG and 2HG5 decreased. For surface properties, as the weight loss increased, MIU increased, but MMD and SMD did not show any trend. For compression properties, LC, WC and RC did not show significant differences according to the degree of weight loss. In case of hand value, Koshi(stiffness), Hari(anti-drape stiffness), Kisimi(scrooping feeling) and Shinayakasa(flexibility with soft feeling) showed a meaningful results depending on the degree of weight loss. However, Shari(crispness) and Fukurami(fullness and softness) did not show meaningful result. Koshi, Hari and Shinayakasa are high correlation with tensile property, bendibg property shear properties, thickness and weight. Kisimi is high correlation with tensile property, bending property, thickness and weight. Shari, Fukurami and THV did not show any meaningful difference whth any mechanical properties.

  • PDF

Studies on the Effect of Korean Ginseng Components on Acetic acid Fermentation. [II] (인삼성분이 초산발효에 미치는 영향에 관한 연구(제2보))

  • 남성희;유태종
    • Journal of Ginseng Research
    • /
    • v.4 no.2
    • /
    • pp.133-145
    • /
    • 1980
  • In order to find out the inhibitors of acetic acid fermentation in Korean ginseng (Panax Sin son C. A. Meyer), total aglycone, panaxadiol, panaxadiol, oleanolic acid and ${\beta}$ -sitosterol were added to the basal medium, respectively, and a surface culture was carried out at 30$^{\circ}C$. The results were as follows: 1 . Saponins lost their activity to inhibit the acetic acid fermentation by hydrolysis. 2 Panaxadiol inhibited slightly, and the degree of inhibition was about 1/300 of that of free saponins. 3. Panaxadiol and oleanolic acid inhibited silighly similar to total aglycone. 4. Acetic acid fermentation was stimulated at the early stage when ${\beta}$-sitosterol was added to the media below the level of 0.000815%. But the fermentation was inhibited when media contained it more than that media 5. An over-oxidation of acetic acid was observed when the media contained total aglycone. panaxadiol, panaxatriol, oleanolic acid and ${\beta}$-sitosterol, respectively, while the media which contained sucrose, ginseng extracts ginseng saponins was shown not to be over-oxidized.

  • PDF

A Study on the Decomposition of Amygdalin Using an In Vitro Assay (Amygdalin의 in Vitro 분해에 관한 연구)

  • Kwon, Hoon-Jeong;Jo, Yong-Jin
    • Toxicological Research
    • /
    • v.23 no.1
    • /
    • pp.47-53
    • /
    • 2007
  • Amygdalin is a cyanogenic glycoside which is commonly found in almonds, bamboo shoots, and apri-cot kernels, and peach kernels. Amygdalin was first hydrolysed into prunasin, then degraded into cyanohydrin by sequential two-stage mechanism. The objective of this study was to examine the amygdalin decomposition and cyanide formation at various in vitro conditions, including acid, enzyme and anaerobic microbes (AM) in human feces (HF). In acid hydrolysis mimicking gastric environment, amygdalin was degraded to cyanide up to 0.2% in specific pH. In contrast, enzyme assay showed higher cyanide generation either by ${\beta}$-glucosidase, or by incubation with microbe. In conclusion, we are convinced of cyanide generation are occurred mainly by microbiological activities of the gut flora up to 41.53%. After ingestion with some staff, the degree and site of degradation in an organism is a key parst of regulatory decision making of that staff.

Effects of ${\alpha}$-Chymotrypsin Modification on the Functional Properties of Soy Protein Isolates

  • Ahn Tae-Hyun;Lee Sook-Young
    • KOREAN JOURNAL OF CROP SCIENCE
    • /
    • v.51 no.2
    • /
    • pp.148-153
    • /
    • 2006
  • Effects of ${\alpha}$-chymotrypsin modification on degree of hydrolysis (DH), solubility, emulsifying capacity and thermal aggregation of laboratory-purified soy protein isolate (SPI) using a lipoxygenase-defected soybean (Jinpum-kong) and commercial soy protein isolate (Supro 500E) were compared. SPIs were hydrolyzed by ${\alpha}$-chymotrypsin at pH 7.8 and $37^{\circ}C$ for 30 min. DHs of Supro 500E and Jinpum-kong SPI were increased by ${\alpha}$-chymotrypsin modification, and DH of Supro 500E was higher than that of Jinpum-kong SPI. DH of ${\alpha}$-chymotrypsin treated Jinpum-kong SPI was similar with untreated Supro 500E and DH of treated Supro 500E was the highest. Solubility, emulsifying capacity and thermal aggregation of SPIs were increased by ${\alpha}$-chymotrypsin modification, and these changes were highly related to changes in DH. Functional properties of Supro 500E were higher than Jinpum-kong SPI in both of untreated and ${\alpha}$-chymotrypsin treated SPIs.

Preparation of Polystyrene particles based on interfacial stability of suspension polymerization (현탁중합의 계면안정에 따른 폴리스티렌 입자 제조)

  • 이진호;이상남;박문수;김은경;문명준
    • Journal of the Korean Graphic Arts Communication Society
    • /
    • v.20 no.1
    • /
    • pp.65-78
    • /
    • 2002
  • The suspension polymerization of styrene was carried out to obtain the narrow-size distribution of particle by using poly(vinyl alcohol) (PVA) as suspension stabilizer according to the degree of hydrolysis and the molecular weight. The stabilizing properties of suspension are also dependent on the interfacial tension of aqueous solution when PVA is added. When the polymerization process was carried out with low hydrolyzed PVA, it gave single, well-defined particles, while high hydrolyzed PVA gave clusters. The size of particle produced in this study ranged between 5${\mu}{\textrm}{m}$ and 10${\mu}{\textrm}{m}$. The suspending agent, PVA, influences on the drop size and drop stability, When the molecular weight of PVA is increased, the drop size decreases and the drops become more stable toward coalescence. An increase in the PVA concentration decreases the mean drop size and narrows the drop size distribution.

  • PDF

A Study on the Early Hydration-Retarding Mechanism of Polymer Modified Cement (Polymer Modified Cement의 초기 수화 지연 mechanism에 관한연구)

  • Kang, Seung-Min;Kang, Hyun-Ju;Song, Myong-Shin;Park, Phil-Hwan
    • Proceedings of the Korea Concrete Institute Conference
    • /
    • 2009.05a
    • /
    • pp.221-222
    • /
    • 2009
  • The results showed that the addition of VAE polymer strongly reduces the $Ca(OH)_2$ formation, being this result attributed to reduce degree of cement hydration caused by different ion elution amount of polymer modified cement pastes and interaction between acetate anion from the partial hydrolysis of co-polymer and Ca$^{2+}$ion from OPC hydration.

  • PDF

Production and Characteristics of Enzymatically Hydrolyzed Soy Sauce by the Treatment Using Proteases (단백질 분해효소로 원료 처리하여 제조한 효소분해 간장의 특성)

  • 채희정;인만진;김민홍
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.26 no.5
    • /
    • pp.784-787
    • /
    • 1997
  • Enzymatically hydrolyzed soy sauce(eHSS) was prepared by the treatment of defatted soy flake using two types of proteases, followed by maillard reaction and formulation with some ingredients. The eHSS was mixed with fermented soy sauce(FSS) to make enzymatically hydrolyzed mixed soy sauce(eHMSS). The properties and sensory characteristics were evaluated and compared with commercially available soy sauces. The control of salt and total nitrogen contents in eHSS and eHMSS was easy, and the production of soy sauce of low salt and high protein was possible. However, the free amino acid content of eHSS was lower than FSS. due to lower degree of hydrolysis. In sensory evaluation, the eHSS have no loss taste and overall acceptance than FSS. Consequently, the eHSS and eHMSS have the potential for use with FSS to produce high quality soy sauce of low salt and high protein contents.

  • PDF

해양에서 분리한 Bacillus subtilis SH-1이 분비하는 용균효소의 정제 및 특성

  • 진성현;정영기;류병호
    • Microbiology and Biotechnology Letters
    • /
    • v.24 no.2
    • /
    • pp.191-196
    • /
    • 1996
  • The bacteriolytic enzyme produced from Bacillus subtilis SH-1 was purified and characterized, and its molecular weight was determined. The bacteriolytic enzyme activity was increased about 66.5 times via purification with recovery yield of 18.5%. The optimum pH and temperature of this enzyme were 9.0 and 50$\circ$C. The enzyme was stable within a pH range of 6.0-10.0 and unstable above 60 . The molecular weight of the enzyme was estimated to be 23,000 dalton in a form of monomer with no other subunits. Effect of the enzyme on the lysis of bacteria engaged in food posion was tested. The lysis degree was below 31% against Gram negative bacteria and above 48% in Gram positive bacteria. The values higher than 73% were obtained against Vibrio sp. and Listeria sp. As the turbidity of dissolved peptidoglycan clecreases, the free amino group levels were increased. And, based on hydrolysis of casein, this enzyme was thought to be an endopeptidase.

  • PDF