• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2005년도 추계종합학술대회
• /
• pp.755-758
• /
• 2005

This paper describes the novel immunoassay sensing system for a portable clinical diagnosis system. It consists of a bead cage reactor and a CMOS integrated biosensor. It showed the simple and easy antibody coating method on beads by flow-through avidin biotin complex technology in a microfluidic device. It showed just 90 nL sample consumption and good result for the application of alpha feto protein. The bead cage reactor has the role of the antibody coating, antigen binding and enzyme linking for the electrochemical sensing method. The CMOS biosensor consists of ISFET (ion selective field effect transistor) biosensor and temperature sensor for detecting pH that is the byproduct of enzyme reaction. The sensitivity is 8 $kHz/^{\circ}C$ in a temperature sensor and 33 mV/pH in a pH sensor. After filling the 15 um polystyrene beads in bead cage, antibody flowed and reacted to beads. Subsequently, the biotinylated antigen flowed and bound to the antibody and GOD (glucose oxidase)-avidin conjugate flowed and reacted to the biotin of the biotinylated antigen. After this reaction process, glucose solution flowed and reacted to the GOD on beads. The hydrogen was generated by glucose-GOD reaction. And it was detected by the pH sensor.

• 센서학회지
• /
• 제10권4호
• /
• pp.259-265
• /
• 2001

FET형 용존산소 센서는 pH-ISFET를 기본 소자로하여 pH 감지 게이트 근처에 백금으로 된 작업 전극을 형성한 구조를 가지고 있다. 작업전극에 특정한 전위를 인가하면 전기분해로 인하여 용존산소의 농도에 비례하는 수소 이온이 pH 감지 게이트 주변에 발생된다. 따라서 pH-ISFET를 통하여 수소 이온 농도의 변화량을 검출하면 용존산소의 농도를 측정할 수 있게된다. 본 논문에서는 이러한 FET형 용존산소 센서를 어레이 형태로 제작하여 적용하고 측정의 신뢰성을 높이기 위한 알고리즘을 도입한 FET형 용존산소 센서 어레이 측정 시스템을 개발하였다. 또한 상용 용존산소 측정기와 그 성능을 비교 분석하였다.

• 대한화학회지
• /
• 제35권1호
• /
• pp.51-58
• /
• 1991

새로운 管形 PVC膜 질산이온 選擇性 電極을 제작하여 그 性能을 조사하였다. 電極膜의 組成을 5${\%}$ aliquat-NO$_3$ 용액+ 32${\%}$ poly(vinyl chloride) + 63${\%}$ dibutyl sebecate로 하였을 때 전극의 感應特性이 최적이었으며, 이 때의 감응기울기는 58.5 ${\pm}$ 0.1 mV/decade, 檢出限界는 0.02mM, 感應時間 (t$_{99}$)은 25초이었다. 이 전극을 써서 아질산이온을 連續${\cdot}$自動化法으로 정량하였다. Recipient 용액은 pH 7.6의 10$^{-2}$ M 燐酸鹽 緩衝溶液을 쓰고, 여기에 酸化劑로서 過酸化水素를 0.3${\%}$가 되도록 포함시켰다. 전극계의 最適條件에서 아질산이온 標集溶液 8.0 ${\times}$ 10$^{-5}$ M ∼ 5.0 ${\times}$ 10$^{-2}$ M의 범위에서 직선적인 감응을 나타내었으며, 이 때의 감응기울기는 56.8 ${\pm}$ 0.2 mV/decade이었다.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2012년도 제43회 하계 정기 학술대회 초록집
• /
• pp.222-222
• /
• 2012

Metal oxide gas sensors based on semiconductor type have attracted a great deal of attention due to their low cost, flexible production and simple usability. However, most works have been focused on n-type oxides, while the characteristics of p-type oxide gas sensors have been barely studied. An investigation on p-type oxides is very important in that the use of them makes possible the novel sensors such as p-n diode and tandem devices. Monoclinic cupric oxide (CuO) is p-type semiconductor with narrow band gap (~1.2 eV). This is composed of abundant, nontoxic elements on earth, and thus low-cost, environment-friendly devices can be realized. However, gas sensing properties of neat CuO were rarely explored and the mechanism still remains unclear. In this work, the neat CuO layers with highly ordered mesoporous structures were prepared by a template-free, one-pot solution-based method using novel ink solutions, formulated with copper formate tetrahydrate, hexylamine and ethyl cellulose. The shear viscosity of the formulated solutions was 5.79 Pa s at a shear rate of 1 s-1. The solutions were coated on SiO2/Si substrates by spin-coating (ink) and calcined for 1 h at the temperature of $200{\sim}600^{\circ}C$ in air. The surface and cross-sectional morphologies of the formed CuO layers were observed by a focused ion beam scanning electron microscopy (FIB-SEM) and porosity was determined by image analysis using simple computer-programming. XRD analysis showed phase evolutions of the layers, depending on the calcination temperature, and thermal decompositions of the neat precursor and the formulated ink were investigated by TGA and DSC. As a result, the formation of the porous structures was attributed to the vaporization of ethyl cellulose contained in the solutions. Mesoporous CuO, formed with the ink solution, consisted of grains and pores with nano-meter size. All of them were strongly dependent on calcination temperature. Sensing properties toward H2 and C2H5OH gases were examined as a function of operating temperature. High and fast responses toward H2 and C2H5OH gases were discussed in terms of crystallinity, nonstoichiometry and morphological factors such as porosity, grain size and surface-to-volume ratio. To our knowledge, the responses toward H2 and C2H5OH gases of these CuO gas sensors are comparable to previously reported values.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2023년도 춘계학술대회
• /
• pp.105-105
• /
• 2023

Heavy metals, including lead (Pb) toxicity, are increasing in soil and are considered toxic in small amounts. Pb contamination is mainly caused by industrialization - smelting, mining. Agricultural practices - sewage sludge, pests and urban practices - lead paint. It can seriously damage and threaten crop growth. Pb can adversely affect plant growth and development by affecting the photosystem, cell membrane integrity, and excessive production of reactive oxygen species (ROS) such as hydrogen peroxide (H₂O₂)andsuperoxide(O₂.-). NO is produced via enzymatic and non-enzymatic antioxidants to scavenge ROS and lipid peroxidation substrates in terms of protecting cells from oxidative damage. Thus, NO improves ion homeostasis and confers resistance to metal stress. Our results here suggest that exogenous NO may aid in better growth under lead stress. These enhancements may be aided by NO's ability in sensing, signaling and stress tolerance in plants under heavy metal stress in combination with lead stress. Our results show that GSNO has a positive effect on soybean seedling growth in response to axillary pressure and that NO supplementation helps to reduce chlorophyll maturation and relative water content in leaves and roots following strong burst under lead stress. GSNO supplementation (200 µM and 100 µM) reduced compaction and approximated oxidative damage of MDA, proline and H₂O₂. Under plant tension, a distorted appearance was found in the relief of oxidative damage by ROS scavenging by GSNO application. In summary, modulation of these NO, PCS and prolongation of metal past reversing GSNO application confirms the detoxification of ROS induced by toxic metal rates in soybean. In summary, these NO, PCS and metal traditionally sustained rates of reverse GSNO application confirm the detoxification of ROS induced by toxic metal rates in soybean.