• 한국수산과학회지
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• 제50권5호
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• pp.520-526
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• 2017

Carnosine was recently reported to protect against the DNA damage induced by oxidative stress. In this study, we investigated the protective effect of eel Anguilla japonica carnosine extracts prepared using different methods (heat treatment extracts, HTEs; ion exchange chromatography, IEC; ultrafiltration permeation, UFP) on leukocyte DNA damage using the comet assay. Human leukocytes were incubated with extracts of eel carnosine at concentrations (of 10, 50, $100{\mu}g/mL$), and then subjected to an oxidative stimulus [$200{\mu}M$ hydrogen peroxide ($H_2O_2$)]. Pretreatment of the cells for 30 min with carnosine significantly reduced the genotoxicity of $H_2O_2$ measured as DNA strand breaks. The protective effects of the three types of extract (HTE, IEC, and UFP) increased with concentration. At the highest concentration (100 g/mL). there were no statistical differences in oxidative damage between each extract treatment and PBS-treated negative controls. When leukocytes were incubated with carnosine for 30 min after exposure to $H_2O_2$. the protective ability of each extract changed. Therefore, eel carnosine inhibits the $H_2O_2$ induced damage to cellular DNA in human leukocytes, supporting the protective effect of this compound against oxidative damage.

• 대한원격탐사학회지
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• 제37권3호
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• pp.591-601
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• 2021

국내의 화학 산업이 지속적으로 발전이 이루어짐에 따라 화학물질의 취급량과 운송량은 매년 증가하고 있다. 우리나라 도로 화물운송은 90%이상을 차지하고 있으며, 화학물질 운송도 대부분 도로를 통해 이루어지고 있다. 이러한 화학물질 운송차량들은 사고가 발생하게 되면 대형사고로 이어질 수 있다. 운송차량은 1차 피해인 교통사고뿐만 아니라 2차 피해인 환경 피해 요인들인 수질오염, 토양오염 등을 발생시킬 가능성이 높다. 본 연구는 반포IC와 서초IC 구간을 연구지역으로 설정하여 염화수소 가스 누출에 대한 시나리오를 작성하여, ALOHA 프로그램을 사용하여 예측거리를 측정하고 거리에 따라 염화수소 가스가 도달한 시간을 분석하였다. 또한 GIS를 이용해 시간별로 발생한 피해 영역에 대해서 인구밀도를 이용한 리스크 평가를 수행하였다. 이를 통해 피해 영역에 대해서 예방·대응 방안의 필요성을 제시하였다.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2000년도 추계학술대회 논문집
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• pp.476-479
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• 2000

In this study, mesh-type PECVD system was suggested to minimize the hydrogen concentration. The main structural difference between the triode system and a conventional system is that a mesh was attached to the substrate holding electrode. We investigated several conditions to compare with conventional PECVD. The main effect of mesh was to minimize the substrate damage by ion bombardment and to enhance the surface reaction to induce hydrogen desorption. It was also found that hydrogen concentration decreased but deposition rate increased as increasing applied dias. Applied DC bias enhanced sputtering process. Intense ion bombardment causes the weakly bonded hydrogen or hydrogen-containing species to leave the growing film and increased adatom mobility. Furthermore, addition of hydrogen gas enhance the surface diffusion of adatom. The structural properties of poly-Si films were analyzed by scanning electron microscopy(SEM).

• 한국압력기기공학회 논문집
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• 제19권2호
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• pp.117-129
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• 2023

Hydrogen embrittlement of a pipe is an important factor in hydrogen transport. To characterize hydrogen embrittlement, tensile and fracture toughness tests should be conducted. However, in the case of hydrogen-embrittled materials, it is difficult to perform tests in hydrogen environment, particularly at low temperatures. It would be useful to develop a methodology to predict the fracture toughness of hydrogen-embrittled materials at low temperatures using more efficient tests. In this study, the fracture toughness of API X52 steels in hydrogen at low temperatures is predicted from numerical simulation using coupled finite element (FE) damage analyses with FE diffusion analysis, calibrated by analyzing small punch test data.

• 전자공학회논문지A
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• 제31A권4호
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• pp.63-69
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• 1994

The Ultrahigh Vacuum Electron Cyclotron Resonance Chemical Vapor Deposition(UHV-ECRCVD) system whose base pressure is 1${\times}10^{9}$ torr has been constructed. In-situ cleaning prior to the epitaxial growth was carried out at 56$0^{\circ}C$ by ECR generated uniform hydrogen plasma whose density is $10^{10}/cm{3}$. The natural oxide was effectively removed without damage by applying positive DC bias(+10V) to the substrate. RHEED(Reflection High Energy Electron Diffraction) analysis has been used to confirm the removal of the surgace oxide and the streaky 2$\times$1 reconstruction of the Si surface, and the suppression of the substrate damage is anaylized by X-TEM(cross-sectional Transmission Electron Microscopy). Surface cleaning technique by ECR hydrogen plasma confirmed good quality epitaxial growth at low temperature.

• 한국수소및신에너지학회논문집
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• 제34권2호
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• pp.205-211
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• 2023

Hydrogen gas is light and diffuses very quickly. Therefore, when a leakage accident occurs, the damage is great, so a technology that can quickly measure the leakage in the air at a long distance is needed. In order to develop hydrogen gas leaked in the atmosphere in a non-contact manner, an experiment was performed to measure hydrogen gas using a lidar technology using the Raman effect. Hydrogen Raman signals were detected using a UV LED light source, which is a Raman light source, and a spectrometer in the ultraviolet region including an optical filter in the 400-430 nm band. To develop this, a Raman lidar optical structure was designed to measure the hydrogen Raman signal at a certain distance, and the hydrogen Raman spectrum was confirmed using a standard gas to evaluate the performance of this optical structure. The linearity was found to be 0.99 using hydrogen standard gas (10, 50, 100, 500, 1,000 ppm). Accordingly, a Raman lidar capable of measuring hydrogen gas rapidly diffusing in the air in an open state was developed to improve the limitations of existing hydrogen sensors.

• 공업화학
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• 제22권5호
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• pp.579-581
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• 2011

과산화수소에 의한 모발의 화학적 손상을 SEM/EDS, CLSM, FT-IR을 통해 평가하였다. SEM/EDS상에서 과산화수소 처리된 모발은 미처리 모발에 비해 표면의 비늘구조 사이의 간격이 벌어졌고, 구성원소를 분석한 결과 황의 비율이 감소하였으며 산소의 비율은 상대적으로 증가하였다. CLSM 관찰 결과 미처리 모발은 강한 형광을 나타내었지만, 탈색모발은 낮은 형광을 보여주었다. 이는 미처리 모발의 경우 자동형광을 나타낼 수 있는 방향족 아미노산이 많이 존재하는데 비해, 탈색모발은 방향족 아미노산이 파괴되어 형광이 낮게 나온 것으로 볼 수 있다. 또한 FT-IR 분석결과 탈색의 증가에 따라 시스테인산(cysteic acid)이 증가되었으며, 이는 폴리펩타이드 사슬사이에 다리결합을 하고 있는 시스틴의 산화에 의한 것으로 볼 수 있다. 이러한 결과들은 산화적 손상이 S-S 결합을 파괴하고 모발 섬유의 인장력을 낮추어 주는 것을 의미한다.

• 한국가스학회지
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• 제25권2호
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• pp.13-21
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• 2021

최근 수소자동차 보급의 확대로 수소충전소의 설치가 점차 확대될 예정이다. 본 연구에서는 수소충전소의 최악의 상황을 가정한 시나리오를 기반으로 피해규모를 예측하고 보다 안전한 설계형태를 제안하고자 한다. 피해규모 예측 방법은 전산유체역학(CFD)을 이용한 Flacs solver를 사용하였으며 이전 연구자의 실험 결과와 비교하여 정확성을 검증하였다. 피해규모 예측은 수소누출과 폭발에 대해서 실시하였으며, 예측 대상은 실측치를 기반으로 한 KR model로 하였다. 그리고 비교검토 모델로는 천정이 없는 형태인 Roofless model을 선정하였다. 두 모델에 대하여 분석한 결과 KR model에서는 내부가 60 vol% 이상까지 수소가스가 누적·체류되는 현상을 확인 할 수 있었던 반면, Roofless model의 경우에는 누출 후 벽면을 타고 충전소 외부로 방출·확산되는 현상을 확인 할 수 있었다. 결론적으로 국내에 표준모델로 보급·확산되고 있는 수소충전소 모델보다는 천정이 없는 수소 충전소 형태가 안전상 유리한 것으로 검토되었다.

• 한국재료학회지
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• 제3권5호
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• pp.482-496
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• 1993

Radiation damage and contamination of silicons etched in the $CF_4+H_2$ and $CHF_3$ magnetron discharges have been characterized using Schottky diode characteristics, TEM, AES, and SIMS as a function of applied magnetic field strength. It turned out that, as the magnetic field strength increased, the radiation damage measured by cross sectional TEM and by leakage current of Schottky diodes decreased colse to that of wet dtched samples especially for $CF_4$ plasma etched samples, For $CF_4+H_2$and $CHF_3$ etched samples, hydrogen from the plasmas introduced extended defects to the silicon and this caused increased leakage current to the samples etched at low magnetic field strength conditions by hydrogen passivation. The thickness of polymer with the increasing magnetic field strength and showed the minimum polymer residue thickness near the 100Gauss where the silicon etch rate was maximum. Also, other contaminants such as target material were found to be minimum on the etched silicon surface near the highest etch rate condition.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.365-371
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• 2017

Hydrogen has potential for preventive and therapeutic applications as an antioxidant. However, micro- and macroparticles of hydrogen in water disappear easily over time. In order to eliminate reactive oxygen species (ROS) related with the aging process, we used functional water containing nanoparticle hydrogen. Nanoparticle hydrogen does not disappear easily and collapse under water after long periods of time. We used murine embryonic fibroblasts that were isolated from 12.5-day embryos of C57BL/6 mice. We investigated the ability of nanoparticle hydrogen in water to suppress hydroxyurea-induced ROS production, cytotoxicity, and the accumulation of ${\beta}-galactosidase$ (an indicator of aging), and promote cell proliferation. The accumulation of ${\beta}-galactosidase$ in the cytoplasm and the appearance of abnormal nuclei were inhibited by daily treatment of cells with hydrogen water. When the aging process was accelerated by hydroxyurea-induced oxidative stress, the effect of hydrogen water was even more remarkable. Thus, this study showed the antioxidant and anti-senescence effects of hydrogen water. Nanoparticle hydrogen water is potentially a potent anti-aging agent.