• Journal of Electrical Engineering and Technology
• /
• v.3 no.4
• /
• pp.593-598
• /
• 2008

The power distribution is proposed to determine the target operating points of the system components as the basis for maximal the efficiency of the overall system for a power split dual electric machine hybrid electric bus. The coordinated control is constructed on the basis of the power distribution. The basic coordinated control is implemented to satisfy the driver's power demand, in which both the dynamic characteristics of the engine and the dual electric machine are explicitly taken into account. Moreover, the improved coordinated control is suggested to suppress engine dynamic operation and rich fuel injection.

• Journal of Microbiology and Biotechnology
• /
• v.15 no.3
• /
• pp.660-664
• /
• 2005

We have previously isolated specific RNA aptamers with high affinity against the helicase domain of hepatitis C virus (HCV) nonstructural protein 3 (NS3). The RNA aptamers competitively and efficiently inhibited the helicase activity, partially impeding HCV replicon replication in human hepatocarcinoma cells. In this study, the RNA aptamers were tested for binding to the HCV NS3 proteins in eukaryotic cells, using a yeast three-hybrid system. The aptamers were then recognized by the HCV NS3 proteins when expressed in the cells, while the antisense sequences of the aptamers were not. These results suggest that the in vitro selected RNA aptamers can also specifically bind to the target proteins in vivo. Consequently, they could be potentially utilized as anti-HCV lead compounds.

• Journal of Korea Multimedia Society
• /
• v.19 no.9
• /
• pp.1669-1678
• /
• 2016

There are many kinds of military images used in naval combat system because various sensors are operated. The military images are displayed, analysed and stored with analysed informations according to the tactical purpose on combat system. These images are used to target detection, analysis and classification. Thus the analysed information and images must be secured, the information hiding methods are the most eligible solutions to get secured informations and images. In this paper, the hybrid information hiding method based on the characteristics of the military images is proposed and the effectiveness is shown by experiments.

• Progress in Medical Physics
• /
• v.32 no.1
• /
• pp.18-24
• /
• 2021

Purpose: Kilovoltage computed tomography (kV-CT) is essential for radiation treatment planning. However, kV-CT images are significantly distorted by artifacts when a metallic prosthesis is present in the patient's body. Thus, the accuracies of target delineation and treatment dose calculation are inevitably lowered. We evaluated the accuracy of the calculated doses using an image restoration method with hybrid CT, which was introduced in our previous study. Methods: A cylindrical phantom containing four metals, namely, silver, copper, tin, and tungsten, was scanned using kV-CT and megavoltage CT to produce hybrid CT images. We created six verification plans for three head and neck patients on kV-CT and hybrid CT images of the phantom and calculated their doses. The actual doses were measured with film patches during beam delivery using tomotherapy. We used the gamma evaluation method to compare dose distribution between kV-CT and hybrid CT with three gamma criteria, namely, 3%/3 mm, 2%/2 mm, and 1%/1 mm. Results: The gamma pass rates decreased as the gamma criteria were strengthened, and the pass rate of hybrid CT was higher than that of kV-CT in all cases. When the 1%/1 mm criterion was used, the difference in gamma pass rates between them was up to 13%p. Conclusions: According to our findings, we expect that the use of hybrid CT can be a suitable approach to avoid the effect of severe metal artifacts on the accuracy of dose calculation and contouring.

• BMB Reports
• /
• v.34 no.1
• /
• pp.59-65
• /
• 2001

In subtractive hybridization, target sequences in the tester are enriched by hybridizing with an excess amount of driver, followed by removing the tester hybridized with the driver. All of existing subtractive cloning methods are designed to remove the tester/driver hybrid. The removal of hybrid, however, is often unsatisfactory For various reasons. In this study we developed a subtractive enrichment protocol in which the tester/driver can be completely removed by selecting only the tester/tester after hybridization. In this protocol both the tester and driver DNAs are ligated with same linker DNAs and amplified by polymerase chain reaction (PCR). The tester DNA is then digested with two different enzymes and used in subsequent hybridization with an excess driver. After hybridization, the DNA is ligated with the adaptor that is only compatible with the tester/tester. Since only the tester/tester can have the new adaptor, no tester/driver can be amplified by PCR in this protocol. Unlike other methods, a 100% subtraction efficiency can be achieved even though the enzymatic treatments used in the enrichment procedure are incomplete. Furthermore, only the hybridized tester DNA can have the new adaptor and be amplified by PCR, resulting in 100% denaturation in effect. The efficacy of this novel method was verified with the model system in which a known amount of the target sequence is included.

• BMB Reports
• /
• v.36 no.4
• /
• pp.344-348
• /
• 2003

Protein kinase CKII is composed of two catalytic ($\alpha$ or $\alpha$') subunits and two regulatory ($\beta$) subunits. The $CKII{\beta}$ subunit is thought to mediate the tetramer formation and interact with other target proteins. However, its physiological function remains obscure. In this study, point mutants of $CKII{\beta}$ that are defective for the L41 binding were isolated by using the reverse two-hybrid system. A sequence analysis of the point mutants revealed that Asp-26, Met-52, and Met-78 of $CKII{\beta}$ are critical for L41 binding; Asn-67 (and/or Lys-139) and Met-52 are important for $CKII{\beta}$ homodimerization. Two point mutants, R75 and R83, of $CKII{\beta}$ interacted with L5, topoisomerase $II{\beta}$, and CKBBP1/SAG, but not with the wild-type $CKII{\beta}$. This indicates that $CKII{\beta}$ homodimerization is not a prerequisite for its binding to target proteins. These $CKII{\beta}$ point mutants may be useful in exploring the biochemical physiological functions of $CKII{\beta}$.

• Journal of the Korean institute of surface engineering
• /
• v.44 no.6
• /
• pp.269-276
• /
• 2011

Phase evolution and tribological behavior of Cr-Mo-N multi compositional films with different interlayer were investigated. The films were deposited by hybrid PVD (Physical Vapor Deposition) system consisted of dc unbalanced magnetron (UBM) sputtering and arc ion plating (AIP) sources. A pure molybdenum (Mo) was used as sputtering target and also a pure Cr was used as AIP target to form the Cr-Mo-N films. Various growth planes were found, no textured surface, in all of the multi composition films. Maximum value of microhardness was measured in Cr-Mo-N film with Mo interlayer as 29 GPa. Composition film was mainly showed the aspect of the adhesive wear than CrN film. The friction coefficient was decreased from 0.6 for pure CrN coating to 0.35 for Cr-Mo-N film with Mo interlayer. This result may come from the formation of metal oxide tribo-layer which is known as solid lubricant during the wear test.

• Proceedings of the KSRS Conference
• /
• 2005.10a
• /
• pp.571-574
• /
• 2005

In this paper, we propose an efficient image segmentation technique for large-sized monochrome images using a hybrid approach which combines threshold and region-based techniques. First, an image is partitioned into fixed-size blocks and for each block the representative intensity is determined by averaging pixel intensities within the block. Next, the neighborhood blocks that have similar characteristics with respect to a specific threshold are merged in order to form candidate regions. Finally, those candidate regions are refined to get final target object regions by merging regions considering the spatial locality and certain criteria. We have performed experiments on images selected from various domains and showed that our technique was able to extract target object regions appropriately from most images.

• Smart Structures and Systems
• /
• v.25 no.3
• /
• pp.323-335
• /
• 2020

Magnetorheological elastomer (MRE), a smart material, is an innovative material for base isolation system. It has magnetorheological (MR) effect that can control the stiffness in real-time. In this paper, a new hybrid base isolation system combining two electromagnetic closed circuits and the roller bearing is proposed. In the proposed system, the roller part can support the vertical load. Thus, the MRE part is free from the vertical load and can exhibit the maximum MR effect. The MRE magnetic loop is constructed in the free space of the roller bearing and forms a strong magnetic field. To demonstrate the performance of the proposed hybrid base isolation system, dynamic characteristic tests and performance evaluation were carried out. Dynamic characteristic tests were performed under the extensive range of strain of the MRE and the change of the applied current. Performance evaluation was carried out using the hybrid simulation under five earthquakes (i.e., El Centro, Kobe, Hachinohe, Northridge, and Loma Prieta). Especially, semi-active fuzzy control algorithm was applied and compared with passive type. From the performance evaluation, the comparison shows that the new hybrid base isolation system using fuzzy control algorithm is superior to passive type in reducing the acceleration and displacement responses of a target structure.

• Journal of the Korean Recycled Construction Resources Institute
• /
• v.9 no.3
• /
• pp.311-321
• /
• 2021

Many studies have been performed on hybrid fiber reinforced concrete for years, which is to improve some of the weak material properties of concrete. Studies on characteristics of hybrid fiber reinforced concrete using amorphous steel fiber and organic fiber, however, yet remain to be done. The purpose of this research is to evaluate the compressive and tensile behaviors and then propose a material model of high performance hybrid fiber reinforced concrete using amorphous steel fiber and polyamide fiber. For this purpose, the high performance hybrid fiber reinforced concretes were made according to their total volume fraction of 1.0% for target compressive strength of 40MPa and 60MPa, respectively, and then the compressive and tensile behaviors of those were evaluated. Also, based on the experimental results of the high performance hybrid fiber reinforced concrete and mortar, each material model for the compressive and tensile behavior was suggested. It was found that the experimental results and the proposed models corresponded relatively well.