• Food Science of Animal Resources
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• v.40 no.1
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• pp.132-144
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• 2020

The aim of this study was to optimize staining procedures for muscle fiber typing efficiently and rapidly in bovine and porcine skeletal muscles, such as longissimus thoracis, psoas major, semimembranosus, and semitendinosus muscles. The commercially available monoclonal anti-myosin heavy chain (MHC) antibodies and fluorescent dye-conjugated secondary antibodies were applied to immunofluorescence histology. Two different procedures, such as cocktail and serial staining, were adopted to immunofluorescence analysis. In bovine muscles, three pure types (I, IIA, and IIX) and one hybrid type, IIA+IIX, were identified by the cocktail procedure with a combination of BA-F8, SC-71, BF-35, and 6H1 anti-MHC antibodies. Porcine muscle fibers were typed into four pure types (I, IIA, IIX, and IIB) and two hybrid types (IIA+IIX and IIX+IIB) by a serial procedure with a combination of BA-F8, SC-71, BF-35, and BF-F3. Unlike for bovine muscle, the cocktail procedure was not recommended in porcine muscle fiber typing because of the abnormal reactivity of SC-71 antibody under cocktail procedure. Within the four antibodies, combinations of two or more anti-MHC antibodies allowed us to distinguish pure fiber types or all fiber types including hybrid types. Application of other secondary antibodies conjugated with different fluorescent dyes allowed us to get improved image resolution that clearly distinguished hybrid fibers. Muscle fiber characteristics differed depending on species and muscle types.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.7A
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• pp.477-484
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• 2003

In this paper, we compared and analyzed the influence of the fading index and the number of branch of diversity in the Nakagami-m fading channel environment when the MRC diversity and the hybrid SC/MRC are applied to the DS/CDMA system. We considered that the number of branches were 2,3, and 4, respectively and applied an individual diversity type according to the number of branches, when nakagami fading index(m) were 1 and 2. We found that when the Nakagami fading index m is small, that is to say, when fading is inferior, the application of a hybrid SC/MRC diversity Rake receiver is efficient; however, when the fading environment is good, the application of the MRC diversity Rake receiver is efficient. As a result, we verified that when the actual communication environment is inferior, the hybrid SC/MRC diversity that selects a strong signal to be received on an individual number of branches could replace the MRC diversity, as a method to achieve a similar performance by reducing the complexity of the system.