• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1989년도 춘계학술대회
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• pp.29-34
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• 1989

300 MHz proton NMR spectra of human blood plasma were analyzed by deconvolution of spectrum, and we compared its results with Fossel's test in normal (15 cases), liver cancer (14 cases) , and other cancer (14 cases) groups. This analysis had enabled us to obtain dynamic characteristics of each individual lipoprotein. As a result of deconvolution method, the VLDL and chylomicron intensity level were found to be elevated in the patients with liver cancer. Moment ratio values of $CH_2$ resonance in the raw spectrum were found to be higher than the normal group for patients with, malignant tumors other than liver cancer. These differences between the three groups could not be found in the conventional Fossel's test. We could simulate plasma spectra by addition of spectra of individual lipoproteins through deconvolution method. Further clinical trials in larger populations and additional biochemical method may shed new light on many of clinical and biochemical interests for knowing characteristics about lipoprotein not separated from blood and the background of Fossel test.

• 한방재활의학과학회지
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• 제26권1호
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• pp.49-61
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• 2016

Objectives To analyze the latest trend through the domestic studies of obesity and support further studies and clinical trials. Methods The theses were searched through KIOM OASIS (http://oasis.kiom.re.kr), The Journal of Society of Korean Medicine for Obesity Research, The Journal of Korean Medicine Rehabilitation. Then, domestic studies were gathered and classified by year, journal and type. Results 92 theses were included. The theses were constantly published every year. According to the analyzing by journals, the most published journal was The Journal of Society of Korean Medicine for Obesity Research. Through the analysis of type, 19 clinical studies and 11 case reports were found. The result of 43 theses analyzing survey and examination numerical value, the study of relationship between obesity and body index is the most published. Among 19 literary studies, 11 studies were searched domestic thesis, 2 studies were searched foreign thesis, 5 studies were searched domestic and foreign thesis simultaneously. Conclusions We need to effort to supplement the statistical error and the topic needs to be diverse. The sustainable research of The human body-oriented obesity studies is required.

• Archives of Pharmacal Research
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• 제18권6호
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• pp.396-401
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• 1995

As a part of trials to develop the antitumor agent from tannins isolated from plants, the antitumor activity of peduculagin, an ellagitannin, isolated from Alnus hirsuta var. microphylla was examined in vitro and in vivo. In vitro, the cytotoxicity was determined by 0.4% typanblue dye exclusion method. peduculagin showed the dose-dependent cytotoxicity against human chronic myelogenous leukemia (K-562), human promyelocytic leukemia (HL-60), mouse lymphoid neoplasm (P388), mouse lymphocytic leukemia (L1210) and mouse sarcoma 180(S180) cell lines. $ED_{50}\; values\; (ED_{50})$ of each cell line were 5.30, 0.92, 2.78, 9.35 and $1.38 \mug/ml$ respectively. The most sensitive cell line was HL-60. In vivo, pedunculagin was administered to ICR mouse with the doses of 50 and $100{\;}{\mu}g/ml$intraperitoneally once at 20 days before S180 inoculation. peduculagin showed the antitumor activity and its T/C ratio (%) was 120.82% in the group of both concentrations.

• IMMUNE NETWORK
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• 제13권6호
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• pp.235-239
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• 2013

Encapsulation of tissue has been an area of intense research with a myriad number of therapeutic applications as diverse as cancer, tissue regeneration, and diabetes. In the case of diabetes, transplantation of pancreatic islets of Langerhans containing insulin-producing beta cells has shown promise toward a cure. However, anti-rejection therapy that is needed to sustain the transplanted tissue has numerous adverse effects, and the islets might still be damaged by immune processes. Furthermore, the profound scarcity of healthy human donor organs restricts the availability of islets for transplant. Islet encapsulation allows the protection of this tissue without the use of toxic medications, while also expanding the donor pool to include animal sources. Before the widespread application of this therapy, there are still issues that need to be resolved. There are many materials that can be used, differing shapes and sizes of capsules, and varied sources of islets to name a few variables that need to be considered. In this review, the current options for capsule generation, past animal and human studies, and future directions in this area of research are discussed.

• 대한인간공학회:학술대회논문집
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• 대한인간공학회 1993년도 춘계학술대회논문집
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• pp.1-9
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• 1993

A human gait study is required for the biomechanical design of running shoes. A tow-dimensional dynamic model was developed in order to analyze lower extremity kinematics and loadings at the right ankle, knee, and hip joints. The dynamic model consists of three segments, the upper leg, the lower leg, and the foot. Each segment was assumed to be a rigid body with one or two frictionless hinge joints. The lower extremity motion was assumed to be planar in the sagittal plane. A young male subject was involved in the gait test and his anthropometric data were measured for the calculation of segement mass and moment of inertia. The experimental data were obtained from three trials of walking at 1.2m/s. The foot-floor reaction data were measured from a Kistler force plate. The kinematic data were acquired using a three-dimensional motion measurement system (Expert Vision) with six markers, five of which were placed on the right lower extremity segments and the rest one was attached to the force plate. Based on the model and experimental data for the stance phase of the right foot, the calculated vertical forces reached up to 492, 540, and 561 N at the hip, knee, ankle joints, respectively. The flexion-extension moments reached up to 155, 119, and 33 Nm in magnitude at the corresponding joints.

• International Journal of Oral Biology
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• 제42권2호
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• pp.71-78
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• 2017

BMP-2 is a well-known TGF-beta related growth factor, having a significant role in bone and cartilage formation. It has been employed to promote bone formation in some clinical trials, and to differentiate mesenchymal stem cells into osteoblasts. However, it is difficult to obtain this protein in its soluble and active form. hBMP-2 is expressed as an inclusion body in the bacterial system. To continuously supply hBMP-2 for research, we optimized the refolding of recombinant hBMP-2 expressed in E. coli, and established an efficient method by using detergent and alkali. Using a heparin column, the recombinant hBMP-2 was purified with the correct refolding. Although combinatorial refolding remarkably enhanced the solubility of the inclusion body, a higher yield of active dimer form of hBMP-2 was obtained from one-step refolding with detergent. The refolded recombinant hBMP-2 induced alkaline phosphatase activity in mouse myoblasts, at $ED_{50}$ of 300-480ng/ml. Furthermore, the expressions of osteogenic markers were upregulated in hPDLSCs and hDPSCs. Therefore, using the process described in this study, the refolded hBMP-2 might be cost-effectively useful for various differentiation experiments in a laboratory.

• Journal of Ginseng Research
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• 제44권2호
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• pp.179-193
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• 2020

Ginseng products on the market show high variability in their composition and overall quality. This becomes a challenge for both consumers and health-care professionals who are in search of high-quality, reliable ginseng products that have a proven safety and efficacy profile. The botanical extract standardization is of crucial importance in this context as it determines the reproducibility of the quality of the product that is essential for the evaluation of effectiveness and safety. This review focuses on the well-characterized and standardized ginseng extract, G115, which represents an excellent example of an herbal drug preparation with constant safety and efficacy within the herbal medicinal products. Over the many decades, extensive preclinical and clinical research has been conducted to evaluate the efficacy and safety of G115. In vitro and in vivo studies of G115 have shown pharmacological effects on physical performance, cognitive function, metabolism, and the immune system. Furthermore, a significant number of G115 clinical studies, most of them double-blind placebo-controlled, have reinforced the findings of preclinical evidence and proved the efficacy of this extract on blood glucose and lipid regulation, chronic obstructive pulmonary disease, energy, physical performance, and immune and cognitive functions. Clinical trials and 50 years of presence on the market are proof of a good safety profile of G115.

• 약학회지
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• 제57권6호
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• pp.412-419
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• 2013

Drug discovery and development processes are time consuming and costly endeavors. It has been reported that on average it takes 10 to 15 years and costs more than $ 1billion to bring a molecule from discovery to market. Compounds fail for various reasons but one of the significant reasons that accounts for failures in clinical trials is poor prediction/understanding of pharmacokinetics and drug metabolism in human. In an effort to improve the number of compounds that exhibit optimal absorption, distribution, metabolism, elimination (ADME), and pharmacokinetic properties in human, drug metabolism, pharmacokinetic scientists have been continually developing new technologies and compound screening strategies. Over the last few years, accelerator mass spectrometry (AMS) and its applications to preclinical/clinical pharmacokinetics and ADME studies have significantly increased, particularly for new chemical/biological entities that are difficult to support with conventional radiolabel studies. In this review, the application of AMS for micro-dosing, micro-tracer absolute bioavailability, mass balance and metabolite profiling studies will be discussed.

• 대한가정학회지
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• 제30권4호
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• pp.15-26
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• 1992

To investigate the seasonal effects on physiological responses of human body, clothing micro-climate, and subjective sensation, selected the cloths the most frequently dressed by men in spring and fall, and completed wearing trials in the climatic chamber. The results are as follows: 1. Rectal temp. ranged 36.8-37.1$^{\circ}C$ in either spring or fall, and no seasonal effect was found. 2. In skin temp., there was no seasonal effect in forehead, abdomen, and forearm. Skin temp. of chest was higher in spring than in fall. On the contrary, reverse was true in high and leg. Average skin temp. ranged 32.2-33.2$^{\circ}C$ in spring and 32.9-34.$0^{\circ}C$ in fall. 3. Average total sweat rate of spring, 79.4g/hr, was smaller than that of fall, 110.9g/hr. 4. Clothing temp. ranged 28.1-32.8$^{\circ}C$ in spring and 27.6-31.$0^{\circ}C$ in fall. Clothing humidity ranged 36.9-48.9% in spring and 38.2-51.1% in fall. Therefore, clothing microclimate was higher during fall than during spring. As results, skin temp. of the body core except chest did not show seasonal variation, but there was obvious seasonal variation in skin temp. of the extremities. Therefore, seasonal variation should be take into consideration in the experiments related to the cloth. In addition, standard for each season and the degree of work performance should be re-established in clothing micro-climate.

• 로봇학회논문지
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• 제10권2호
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• pp.61-69
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• 2015

In this paper, we propose and examine the feasibility of the robot-assisted behavioral intervention system so as to strengthen positive response of the children with autism spectrum disorder (ASD) for learning social skills. Based on well-known behavioral treatment protocols, the robot offers therapeutic training elements of eye contact and emotion reading respectively in child-robot interaction, and it subsequently accomplishes pre-allocated meaningful acts by estimating the level of children's reactivity from reliable recognition modules, as a coping strategy. Furthermore, for the purpose of labor saving and attracting children's interest, we implemented the robotic stimulation configuration with semi-autonomous actions capable of inducing intimacy and tension to children in instructional trials. From these configurations, by evaluating the ability of recognizing human activity as well as by showing improved reactivity for social training, we verified that the proposed system has some positive effects on social development, targeted for preschoolers who have a high functioning level.