• 대한화장품학회지
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• 제23권3호
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• pp.9-23
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• 1997

Retinoids are essential regulators of spithelial cell growth and celluar differentiation. They are also known to be effective in photoaging. It was reported that topical application of retinoic acid improves facial wrinkle carsed by collagen synthesis reduction in photodamaged skin. Collagen synthesis by retinoic acid may contribute to the wrinkle effacement. Since celluar retinoic acid binding protein II is slsctively induced in human skin and dermal fibroblasts in vitro by retinoic acid, this response can be used to mesure retinoids potency and activity. In order to know the activity of retinoids and their relations with collagen synthesis, we treated dermal fibroblasts with retinoids for 48 hours at 10-6-10-7M and measured CRABPII mRNA level by quantitative Nortern blotting. We also measured the rate of collagen systhesis by retinoids using 3-dimensional dermal equivalent. CRABPII mRNA level was increased 3-fold by retinoic acid, 2.1-fold by retinol and 1.4-fold by retinaldehyde. Collagen systhesis was increased 34% by all-trans retinioc acid, 26% by retinol, 17% by retinaldehyde and 7% by retinyl palmitate. From the above results, retinoids were found to be a potent indecers of CRABPII mRNA and collagen synthesis. Though retinoic acid was the most effective, its use has been restricted because of the side effects. Instead, retinol can be a best candidate in cosmetics for the treatment of photodamaged skin in terms of efficacy and safety.

• 통합자연과학논문집
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• 제12권2호
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• pp.47-57
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• 2019

Staphylococcus epidermidis is well-known not only as an innocuous normal flora species commonly isolated from human skin, but also as an important bacterial species to keep skin healthy, because this species can protect the human skin from pathogenic microorganisms. However, S. epidermidis turns into a potential pathogen in damaged skin, because these bacteria can easily form a biofilm on the wound area and provide antimicrobial resistance to other microorganisms embedded in the biofilm. Thus, it is important to kill S. epidermidis in the early stage of wound treatment and block the formation of biofilms in advance. In the present study, hydrogel wound dressings were fabricated using polyvinyl alcohol/polyethylene glycol containing silver citrate nanorods, which have been proven to have strong antimicrobial activity, especially against S. epidermidis, and their wound-healing efficacy was investigated in vivo using a rat experiment.

• Journal of Microbiology and Biotechnology
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• 제33권1호
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• pp.135-141
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• 2023

Protection of skin cells from chronic infrared-A (IRA) irradiation is crucial for anti-photoaging of the skin. In this study, we investigated the protective activity of Rg3(S) and Rg3(S)-incorporated anionic soybean lecithin liposomes (Rg3/Lipo) with a size of approximately 150 nm against IRA-induced photodamage in human fibroblasts. The formulated Rg3/Lipo showed increased solubility in aqueous solution up to a concentration of 200 ㎍/ml, compared to free Rg3(S). In addition, Rg3/Lipo exhibited superior colloidal stability in aqueous solutions and biocompatibility for normal human dermal fibroblasts (NHDFs). After repeated IRA irradiation on NHDFs, elevated levels of cellular and mitochondrial reactive oxygen species (ROS) were greatly reduced by Rg3(S) and Rg3/Lipo. In addition, cells treated with Rg3/Lipo exhibited noticeably reduced apoptotic signals following IRA irradiation compared to untreated cells. Thus, considering aqueous solubility and cellular responses, Rg3/Lipo could serve as a promising infrared protector for healthy aging of skin cells.

• 로봇학회논문지
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• 제18권1호
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• pp.18-28
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• 2023

This study introduces a smart wrist band system with pressure measurements using wrist skin curvature variation due to finger motion. It is easy to wear and take off without pre-adaptation or surgery to use. By analyzing the depth variation of wrist skin curvature during each finger motion, we elaborated the most suitable location of each Force Sensitive Resistor (FSR) to be attached in the wristband with anatomical consideration. A 3D depth camera was used to investigate distinctive wrist locations, responsible for the anatomically de-coupled thumb, index, and middle finger, where the variations of wrist skin curvature appear independently. Then sensors within the wristband were attached correspondingly to measure the pressure change of those points and eventually the finger motion. The smart wrist band was validated for its practicality through two demonstrative applications, i.e., one for a real-time control of prosthetic robot hands and the other for natural human-computer interfacing. And hopefully other futuristic human-related applications would be benefited from the proposed smart wrist band system.

• 한국의학물리학회지:의학물리
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• 제17권2호
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• pp.83-88
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• 2006

목적: 초고자장 MRI/MRS 장비를 이용하여 인체 피부조직의 고해상도 자기공명영상과 자기공명 스펙트럼을 얻고자 하였다. 또한 수분억제가 제외된 자기공명 스펙트럼을 이용하여 스킨로션(피부보습제)의 피부 보습효과가 측정가능한지 알아 보고자 하였다. 대상 및 방법: Bruker 14.1 T MRI/MRS 장비를 사용하였다. 자기공명영상을 얻기 위하여 Spin Echo 및 Fast Spin Echo 펄스시퀀스를 사용하였고 자기공명 스팩트럼을 얻기 위하여 PRESS 펄스시퀀스를 사용하였다. 결과: 초고자장 MRI/MRS 장비를 사용하여 인체 피부에 대한 고해상도의 자기공명영상과 자기공명 스펙트럼을 획득하였다. 수분 신호가 포함된 스펙트럼 분석을 통하여 스킨로션을 바르기 전에 비하여 스킨로션을 바른 후에 인체 피부조직의 수분 함유량이 상당히 증가함이 나타났다. 결론: 본 연구는 14.1 T 고자장 MRI/WRS 장비에서 얻은 피부 조직에 대한 고해상도 자기공명영상과 자기공명 스펙트럼이 보습효과를 측정하는 데 사용될 수 있음을 시사해 주고 있다.

• 한국생활과학회지
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• 제7권1호
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• pp.139-146
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• 1998

The actual clothing conditions of male collegian were surveyed to analyse clothing contents and the rate of wearing underwear. Then, clothing microclimate, physiological responses, and subjective sensations were investigated through wearing trials on human body in climatic chamber based on the results from the survey. The results were follows: 1. Male collegian wore T-shirts, jeans, and socks in summer, and total clothing weight per body surface area was $561g/m^2$. The number of clothes for upper body were 1 layer, but the number of clothes for lower body were 2 layers. Subjective sensations have no significant difference with wearing underwear. 2. Most physiological responses including temperature inside clothing, mean skin temperature, skin temperature of chest, abdomen, thigh, and lower leg, and sweat rate, were higher in with-underwear than in without-underwear. But pulse rates were not significantly different between with-and without-underwear.

• Molecular & Cellular Toxicology
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• 제4권2호
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• pp.150-156
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• 2008

The photoprotective effects of minerals from Korean indigenous ores, consisting mainly of sericite, on UVA-irradiated human dermal fibroblast (HDF) were examined. Zymographic analysis showed that the treatment of the minerals significantly reduced the UVA-enhanced MMP-1 activity and mRNA level. The minerals also showed strong inhibitory effect on MMP-2 activity and mRNA expression. Moreover, the minerals were better than polyphenol in reducing MMP-1 and MMP-2 expressions. Notably, the minerals significantly enhanced collagen biosynthesis in the HDF. Inhibition of the elastase activity and protection against the oxidatively damaged HDF cell were also found in the presence of the minerals. Taken together, the ore minerals may be used as the potent photo-protective and anti-skin-aging ingredients which can prevent skin cell damage by UVA.

• Fibers and Polymers
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• 제4권4호
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• pp.215-221
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• 2003

A vertical skin model with two detachable environmental chambers was developed to simulate a Human-Clothing-Environment system and to evaluate heat and moisture transport properties of textile materials under severe conditions and during transient states. The construction of the system was described and data reproducibility and accuracy of the instrument were verified by using PEG treated nonwovens. Also advantages over a traditional static type experiment were demonstrated based on a series of experiments.

• Reproductive and Developmental Biology
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• 제30권4호
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• pp.229-234
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• 2006

We have generated transgenic mice that expressed mouse extracellular superoxide dismutase (EC-SOD) in their skin. In particular, the expression plasmid DNA containing human keratin K14 promoter was used to direct the keratinocyte-specific transcription of the transgene. To compare intron-dependent and intron-independent gene expression, we constructed two vectors. The vector B, which contains the rabbit -globin intron 2, was not effective for mouse EC-SOD overexpression. The EC-SOD transcript was detected in the skin, as determined by Northern blot analysis. Furthermore, EC-SOD protein was detected in the skin tissue, as demonstrated by Western blot analysis. To evaluate the expression levels of EC-SOD in various tissues, we purified EC-SOD from the skin, lungs, brain, kidneys, livers, and spleen of transgenic mice and measured its activities. EC-SOD activities in the transgenic mice skin were approximately 7 fold higher than in wild-type mice. These results suggest that the mouse overexpressing vector not only induces keratinocyte-specific expression of EC-SOD, but also expresses successfully functional EC-SOD. Thus, these transgenic mice appeared to be useful for the expression of the EC-SOD gene and subsequent analysis of various skin changes, such as erythema, inflamation, photoaging, and skin tumors.

• 한국축산식품학회지
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• 제36권2호
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• pp.237-243
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• 2016

Many researchers revealed that collagen contribute to maintaining the skin’s elasticity and inhibit wrinkling of skin. Korean native cattle (Hanwoo) bone (leg bone, foot and tail) infusion contains the various inorganic materials, collagen and chondroitin sulfate. All of this, a large quantity of collagen is included in Hanwoo infusion. Therefore, this study emphasized on the effects of collagen in the Hanwoo bone infusion. For the first time, Hanwoo bone infusions were directly added to the media of Human Dermal Fibroblast (NHDF-c) to test anti-aging effects. First, it was identified that growth rate of skin fibroblast was increased. Furthermore, the Hanwoo bone infusion increased a 50% of fibroblast collagen synthesis. Also, suppression of skin fibroblast aging was confirmed by treatment Hanwoo bone infusion. In conclusion, this study demonstrates the effects of infusion made from Hanwoo leg bone, foot and tail on anti-aging, wrinkle inhibiting and skin fibroblast elasticity maintaining. Therefore, this study identified that traditional infusion has effects that are good for skin elasticity.